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MSK 2024 International Meeting of the Microbiological Society of Korea PDF Free Download

MSK 2024 International Meeting of the Microbiological Society of Korea PDF free Download. Think more deeply and widely.

www.msk.or.kr | 3

∙2024 MSK Organizing Committee ··························································································· 4

∙General Information ··················································································································· 5

∙Information for Poster Session ································································································ 5

∙Timetable ······································································································································ 6

∙Floor Plans ···································································································································· 7

∙Scientific Program ······················································································································ 8

∙Plenary Lectures ······················································································································· 35

PL1 ··········································································································································· 36

PL2 ··········································································································································· 37

PL3 ··········································································································································· 38

PL4 ··········································································································································· 39

∙MSK Award Lecture ················································································································· 41

AL ············································································································································· 42

∙Luncheon Symposium ··············································································································43

LS1 ··········································································································································· 44

LS2 ··········································································································································· 45

LS3 ··········································································································································· 46

∙Symposia ···································································································································· 47

S1 ············································································································································· 47

S2 ············································································································································· 53

S3 ············································································································································· 59

S4 ············································································································································· 67

S5 ············································································································································· 73

S6 ············································································································································· 79

S7 ············································································································································· 87

S8 ············································································································································· 93

S9 ············································································································································· 99

S10 ········································································································································· 105

S11 ········································································································································· 111

S12 ········································································································································· 117

S13 ········································································································································· 123

S14 ········································································································································· 129

S15 ········································································································································· 135

S16 ········································································································································· 141

S17 ········································································································································· 147

S18 ········································································································································· 153

S19 ········································································································································· 159

S20 ········································································································································· 165

S21 ········································································································································· 171

∙Graduate Students’ Forum ··································································································· 177

GS1 ········································································································································ 178

GS2 ········································································································································ 189

GS3 ········································································································································ 201

GS4 ········································································································································ 213

∙Poster ······································································································································· 225

∙Exhibition ································································································································· 277

∙Author Index ··························································································································· 291

The Microbiological Society of Korea

Rm. 810, The Korea Science Technology Center The first building 22, Teheran-ro 7-gil,

Gangnam-gu, Seoul 06130, Republic of Korea

E-mail: msk@msk.or.kr, Tel: +82-2-3453-3321/86, Fax: +82-2-3453-3322

This work was supported by the Korean Federation of Science and Technology Societies

(KOFST) Grant funded by the Korean Government.

4 | www.msk.or.kr

2024 MSK Organizing Committee

President

Jung-Hyun Lee (KIOST)

General Affairs Committee Finance Committee

Chair Jin-Won Lee (Hanyang Univ.) Chair Sung Ho Yoon (Konkuk Univ.)

Secretary Sang Jun Lee (Chung-Ang Univ.) Secretaries Sang-Wook Han (Chung-Ang Univ.)

Taegun Seo (Dongguk Univ.)

Scientific Program Committee

Chair Ju-Hoon Lee (Seoul Nat’l Univ.)

Secretaries Donghyuk Kim (UNIST)

Keun Pil Kim (Chung-Ang Univ.)

Min-Kyu Kim (KAERI)

Minsik Kim (Yonsei Univ.)

Jae-Ouk Kim (IVI)

Jeong Yoon Lee (Jeonbuk Nat’l Univ.)

Ju Huck Lee (KRIBB)

Bong Hyun Sung (KRIBB)

Tae Woong Whon (WIKIM)

Hyunjin Yoon (Ajou Univ.)

Publication Committee

Chair Yoon-E Choi (Korea Univ.)

Secretaries Yoonjee Chang (Kookmin Univ.)

Yong-Joon Cho (Kangwon Nat’l Univ.)

Bong-Soo Kim (Hallym Univ.)

Jeong-Mok Kim (Hanyang Univ.)

Sangmi Lee (Chungbuk Nat’l Univ.)

Tae Kwon Lee (Yonsei Univ.)

Editorial Committee of Journal of Microbiology

Chair You-Hee Cho (CHA Univ.)

Secretaries Jang-Cheon Cho (Inha Univ.)

Ki Jun Jeong (KAIST)

Keun Pil Kim (Chung-Ang Univ.)

Jin-Won Lee (Hanyang Univ.)

Joon-Hee Lee (Pusan Nat’l Univ.)

Tae Kwon Lee (Yonsei Univ.)

Jinjong Myoung (Jeonbuk Nat’l Univ.)

Woojun Park (Korea Univ.)

Yoonkyung Park (Chosun Univ.)

Woo Jun Sul (Chung-Ang Univ.)

Editorial Committee of Korean Journal of Microbiology

Chair Joon-Hee Lee (Pusan Nat’l Univ.)

Secretaries Jeong-Mok Kim (Hanyang Univ.)

Kyoung-Ho Kim (Pukyong Nat’l Univ.)

Jung Eun Park (Chungnam Nat’l Univ.)

Eun Ju Yun (Jeonbuk Nat’l Univ.)

Policy and Planning Committee External Relations Committee

Chair Hyeun Bum Kim (Dankook Univ.) Chair Hana Yi (Korea Univ.)

Secretaries Changhan Lee (Ajou Univ.)

Moo-seung Lee (KRIBB)

Nam Su Oh (Korea Univ.)

Secretaries Chang-Ro Lee (Myongji Univ.)

Bum Soo Park (Hanyang University)

Hakdong Shin (Sejong Univ.)

Industrial-Educational-Institutional Cooperation Committee

Chair Byung-Yong Kim (CKD Healthcare)

Secretaries Guihwan Han (CIALM)

Jongbum Jeon (KRIBB)

Ok-Sun Kim (KOPRI)

Baekrock Oh (KRIBB)

Kyoung Jin Oh (CJ Bioscience)

Seung-Hwan Park (KRIBB)

Misun Yun (WIKIM)

Public Affairs Committee Committee for Future MSK

Chair Jang Won Yoon (Kangwon Nat’l Univ.) Chair Hak-Jong Choi (WIKIM)

Secretaries Dae Wi Kim (Jeonbuk Nat’l Univ.)

Jae-Kyung Kim (KAERI)

Yoon-Mo Yang (Hanyang University)

Secretaries Jeonghwan Jang (Jeonbuk Nat’l Univ.)

Kyoung-Dong Kim (Chung-Ang Univ.)

Soon-Kyeong Kwon (Gyeongsang Nat’l Univ.)

Awarding Committee Next Term Preparing Committee

Chair Jeong-Yoon Kim (Chungnam Nat’l Univ.) Chair Jin-Woo Bae (Kyung Hee Univ.)

Secretary Yoonkyung Park (Chosun Univ.) Secretary Jung-Shin Lee (Kangwon Nat’l Univ.)

* CIALM : Center for Industrialization of Agricultural and Livestock Microorganisms

IVI : International Vaccine Institute KAERI : Korea Atomic Energy Research Institute

KAIST : Korea Advanced Institute of Science and Technology KOPRI : Korea Polar Research Institute

KRIBB : Korea Research Institute of Bioscience and Biotechnology UNIST : Ulsan National Institute of Science and Technology

WIKIM : World Institute of Kimchi

www.msk.or.kr | 5

General Information

Registration

Place: ICCJEJU 3rd Floor Lobby

Registration fee:

(KRW)

Member Non-Member

Regular Student Regular Student

Pre-Registration 220,000 130,000 300,000 150,000

On-Site Registration 290,000 160,000 370,000 180,000

Paid-

Workshop

R Course – Basic

40,000 (each)

Literature and Sequence Databases

Beginner’s Guide to the Bacterial Genome Analysis

Modeling and Simulation of Metabolic Networks

Utilization of Protein Structure Prediction

Information for Poster Session

Poster Session

Schedule Topics Presentation

Session 1 Set-up : October 30, 13:00

Removal: October 31, 11:00 B, C, G Oct 30 (Wed) 18:00-19:30

Session 2 Set-up : October 31, 13:00

Removal: November 1, 11:00 A, D, E, F, H Oct 31 (Thu) 18:00-19:30

Poster Topics

A. Systematics /

미생물분류

B. Ecology and Environmental Microbiology /

생태

･

환경미생물

C. Applied Microbiology /

응용미생물

D. Immunology and Microbial Pathogenesis /

면역

･

병원미생물

E. Physiology and Biochemistry /

생리

･

생화학

F. Genetics /

미생물유전학

G. Biotechnology /

생물공학

H. Others /

기타

Poster Session Layout

Zone Poster Session 1 Poster Session 2

1 B001-B052 A001-A052

2 B053-B104 A053-A069, D001-D035

3 B105-B143, C001-C013 D036-D084, E001-E003

4 C014-C065 E004-E037, F001-F018

5 C066-C084, G001-G056 F019-F041, H001-H051

6 | www.msk.or.kr

Timetable

Oct 30 (Wed)

Time Halla Hall Samda Hall Rm 303 Rm 302 Rm 301 Lobby

12:00-14:00

GS1

Graduate Students’

Forum 1

GS2

Graduate Students’

Forum 2

GS3

Graduate Students’

Forum 3

[W1_Paid

Workshop]

R Course - Basic

[W2_Paid

Workshop]

Literature and

Sequence Databases

Registration

11:30~

Exhibition

14:00~

14:00-14:10 Break time

14:10-14:50 PL1 - Prof. Michael J. Sadowsky (Halla Hall)

14:50-15:00 Break time

15:00-17:00

Microbiome

Interactions and

Health Impacts:

From Gut to Skin

Host-pathogen

Crosstalk: From

Molecule to

Physiology

Microbial Survival

Systems

Present and Future

of Bacteriophage

Research

Probiotics 2.0:

Exploring the Future

of Next Generation

Probiotics

17:00-17:10 Break time

17:10-17:50 PL2 - Prof. Nobuhiko Kamada (Halla Hall)

18:00-19:30 POSTER Presentation 1 with Standing reception

Oct 31 (Thu)

Time Halla Hall Samda Hall Rm 303 Rm 302 Rm 301 Lobby

09:00-11:00

Engineering Biology:

The Dominant

Approach to

Engineer Biosystems

for Bioeconomy

From Shadow to

Petri Dish:

Breakthroughs in

Culturing Elusive

Microorganisms

Development of

Vaccines against

New and Variant

Infectious Diseases

Agricultural and

Livestock

Industrialization

Based Microbiome

[W3_Paid

Workshop]

Beginner’s Guide to

the Bacterial

Genome Analysis

Registration

08:30~

Exhibition

09:00~

11:00-11:10 Break time

11:10-11:50 PL3 - Dr. John Glass (Halla Hall)

12:00-13:00 Luncheon 1 Luncheon 2 Luncheon 3 LUNCH GM of MSK @

5F Ocean View

13:00-15:00

S10

Microbiome

S11

Engineering of

Biocatalysts:

Enzymes and Cells

S12

Exploring Basic

Mechanisms and

Applications in Viral

Immunology,

Vaccines, and

Therapeutics

S13

Fungal Genetics and

Biology

S14

Unravelling the

Relationship

between the Gut

Microbiome and Skin

Health:

Gut-Skin-Immune

Axis

15:00-15:10 Break time

15:10-15:50 PL4 - Prof. Natalie Strynadka (Halla Hall)

15:50-16:00 Break time

16:00-18:00

S15

Environmental

Microbiology

S16

Structure and

Function of Microbial

Proteins

S17

Innovative

Approaches to

Microbiome-Human

Interactions Using

Next Generation

Sequencing

S18

IVI Session -

Advances in Vaccine

Technology

S19

Bio-Biz Festival

18:00-19:30 POSTER Presentation 2 with Standing reception

Nov 1 (Fri)

Time Halla Hall Samda Hall Rm 303 Rm 302 Rm 301 Lobby

09:00-11:00

S20

Microbial Ecology

S21

Recent Advances in

the Molecular

Biology of Microbes

in Host

Environments

GS4

Graduate Students’

Forum 4

[W4_Paid

Workshop]

Modeling and

Simulation of

Metabolic Networks

[W5_Paid

Workshop]

Utilization of Protein

Structure Prediction

Exhibition

09:00~

11:00-11:10 Break time

11:10-11:50 MSK Award Lecture - Prof. Jang-Cheon Cho (Halla Hall)

12:00-12:30 CLOSING CEREMONY

www.msk.or.kr | 7

Floor Plans

Halla Hall GS1, Plenary Lectures, MSK Award Lecture, Symposia, Closing Ceremony,

LS–CJ Bioscience

Samda Hall GS2, Symposia, LS-Illumina

Room 303 GS3, Symposia, LS-Clinical Microbiomics

Room 302 W1, W4, Symposia

Room 301 W2, W3, W5, Symposia

3rd Floor Lobby Registration Desk, Exhibition, Poster

5th Floor Ocean View General Meeting of MSK

8 | www.msk.or.kr

Scientific Program

Plenary Lectures

PL1 Plenary Lecture 1 Oct 30 (Wed), Halla Hall

Chair: Jeonghwan Jang (Jeonbuk National University)

14:10-14:50

Treating Human Diseases with Bugs and Not Drugs

Michael J. Sadowsky (University of Minnesota, USA)

PL2 Plenary Lecture 2 Oct 30 (Wed), Halla Hall

Chair: Han Young Chung (Chungnam National University)

17:10-17:50

The Mouth and the Gut - Together in Sickness and in Health

Nobuhiko Kamada (University of Michigan, USA)

PL3 Plenary Lecture 3 Oct 31 (Thu), Halla Hall

Chair: Bong Hyun Sung (KRIBB)

Supported by Korea Research Institute of Bioscience and Biotechnology (KRIBB)

11:10-11:50

Investigating the First Principles of Cell Biology Using a Minimal Bacterial Cell

John I. Glass (J. Craig Venter Institute, USA)

Plenary Lecture 4 Oct 31 (Thu), Halla Hall

Chair: Min-Kyu Kim (Korea Atomic Energy Research Institute)

15:10-15:50

Structure-guided Drug Discovery Targeting Cell Wall Antibiotic Resistance Mechanisms in

Staphylococcus aureus

Natalie C.J. Strynadka (University of British Columbia, Canada)

www.msk.or.kr | 9

MSK Award Lecture

2023 Academic Research Awardee Nov 1 (Fri), Halla Hall

Chair: Che Ok Jeon (Chung-Ang University)

11:10-11:50

Oh My Goodness Microbe! - My Journey to Cultivate the Uncultured

Jang-Cheon Cho (Inha University)

Workshops

W1 R Course Workshop (Basic) Oct 30 (Wed), Rm 302

12:00-14:00

R을 활용한 미생물 분석 기초: 자료 가공, ggplot, t-test, ANOVA , 상관관계

Tae Kwon Lee (Yonsei University)

W2 Literature and Sequence Databases Oct 30 (Wed), Rm 301

12:00-14:00

문헌과 서열 정보 데이터베이스 활용

Yoonsoo Hahn (Chung-Ang University)

W3 Beginner’s Guide to the Bacterial Genome Analysis Oct 31 (Thu), Rm 301

09:00-11:00

초보자를 위한 미생물유전체 분석법

Sang-Cheol Park (Korea University)

Modeling and Simulation of Metabolic Networks Nov 1 (Fri), Rm 302

09:00-11:00

대사네트워크 모델링과 시뮬레이션

Sung Ho Yoon (Konkuk University)

W5 Utilization of Protein Structure Prediction Nov 1 (Fri), Rm 301

09:00-11:00

단백질 구조 예측 활용

Kwang-Hyun Park (KRIBB)

10 | www.msk.or.kr

Luncheon Symposium

LS1 Luncheon Symposium 1 Oct 31 (Thu), Halla Hall

12:00-13:00

Introduction to Shotgun Metagenome Analysis and Reporting

Taewook Kim (CJ Bioscience, Inc.)

LS2 Luncheon Symposium 2 Oct 31 (Thu), Samda Hall

12:00-13:00

The Latest Next-generation Sequencing Innovations

Kyoungwoo Jang (Illumina Korea)

LS3 Luncheon Symposium 3 Oct 31 (Thu), Rm 303

12:00-13:00

Introduction of Advanced Microbiome Analysis Software Platform CosmosID-HUB

Seong Min Baek (Clinical Microbiomics)

www.msk.or.kr | 11

Symposia

S1 Microbiome Interactions and Health Impacts: From Gut to Skin

Oct 30 (Wed), Halla Hall

Chair: Doo-Sang Park (KRIBB)

S1-1 15:00-15:30

Akkermansia muciniphila-Derived Amuc_1409 Protein Improves Gut Health through

Intestinal Stem Cell Regulation

Chul-Ho Lee (KRIBB)

S1-2 15:30-16:00

Applications of the Human Gut Microbiome Information in Koreans: Towards Precision

Nutrition and Healthcare

Eun-Ji Song (Korea Food Research Institute)

Chair: Min-Soo Kim (Chungnam National University)

S1-3 16:00-16:30

Skin Microbe-dependent TSLP-ILC2 Priming Axis in Early Life is Co-opted in Allergic

Inflammation

Jimin Cha (Yonsei University College of Medicine)

S1-4 16:30-17:00

Gut Commensal Microbe Regulated by Host Imd Signaling Pathway Accelerates Adult

Emergence in Female Fruit Fly

Hyun Sik Kim (Kyung Hee University)

S2 Host-pathogen Crosstalk: From Molecule to Physiology

Oct 30 (Wed), Samda Hall

Chair: Wonsik Lee (Sungkyunkwan University) & Jinki Yeom (Seoul National University)

S2-1 15:00-15:25

Deciphering the Bacterial-Fungal Crosstalk in the Gut Microbiota with Chemical Biology

Tools

Yuan Qiao (Nanyang Technological University, Singapore)

12 | www.msk.or.kr

S2-2 15:25-15:50

Exploring Host-pathogen Interactions: Unmasking a Salmonella Genotoxin’s Assault on

Mitochondria

Shu-Jung Chang (National Taiwan University, Taiwan)

S2-3 15:50-16:15

Unravelling of Complex Poxvirus Structures Using Cryo-electron Microscopy

Jaekyung Hyun (Sungkyunkwan University)

S2-4 16:15-16:40

Multi-omics Approaches to Enhance Metabolic Activities of Microorganisms

Jungyeon Kim (Seoul National University)

S2-5 16:40-16:55

Intracellular and Intra-organ Host Environments Differentially Shape the Survival Fitness of

Staphylococcus aureus

JuOae Chang (Sungkyunkwan University)

S3 Microbial Survival Systems

Oct 30 (Wed), Rm 303

Co-organized by Science Research Center for Microbial Survival Systems

Chair: Hyun Ah Kang (Chung-Ang University)

S3-1 15:00-15:20

AmiC Modulates RNase E-Mediated Decay of the Small RNA SgrS during Glucose Phosphate

Stress in Escherichia coli

Eunkyoung Shin (Daegu Catholic University)

S3-2 15:20-15:40

An Extracellular Lipoprotein Released from OMV Enhances Biofilm Maturation via Specific

Interaction with Exopolysaccharides

Bo-Ram Jang (Sogang University)

www.msk.or.kr | 13

S3-3 15:40-16:00

Evolutionary Unique N-Glycan-dependent Glycoprotein Quality Control System Is Critical

for Cellular Fitness and Extracellular Vesicle Transport in Cryptococcus neoformans

Catia Mota (Chung-Ang University)

Chair: Che Ok Jeon (Chung-Ang University)

S3-4 16:00-16:20

Direct Interaction of RPA with Rad52 Regulate Meiotic Recombination

Jeong Hwan Joo (Chung-Ang University)

S3-5 16:20-16:40

Elucidation of Core Bacterial Communities and Their Metabolic Characteristics in the

Phycosphere of Marine Algae

Jeong Min Kim (Chung-Ang University)

S3-6 16:40-17:00

The Role of KIF18A in Antiviral Immune Responses

YongHyun Lim (Chung-Ang University)

S4 Present and Future of Bacteriophage Research

Oct 30 (Wed), Rm 302

Sponsored by the National Research Foundation of Korea (NRF) grant,

funded by the Korean government (MSIT) (RS-2024-00342735)

Chair: Minsik Kim (Yonsei University)

S4-1_Keynote Speaker 15:00-15:30

Grand Challenges in Phage and Endolysin Therapy

Sangryeol Ryu (Seoul National University)

S4-2 15:30-15:55

Defense Mechanisms against RNA Phages in Pseudomonas aeruginosa

You-Hee Cho (CHA University)

14 | www.msk.or.kr

S4-3 15:55-16:25

Phage-based Strategies for Targeting Refractory Bacterial Infections

Longzhu Cui (Jichi Medical University, Japan)

S4-4 16:25-16:45

CRISPR-Cas System: From Genome Editing to Bacterial Attack

Sang Jun Lee (Chung-Ang University)

S4-5 16:45-17:05

Innovative Strategies for Enhancing the Efficacy of Bacteriophage and Endolysin

Jaewoo Bai (Seoul Women’s University)

S5 Probiotics 2.0: Exploring the Future of Next Generation Probiotics

Oct 30 (Wed), Rm 301

Sponsored by BioBankHealing Corp.

Chair: Nam Su Oh (Korea University)

S5-1 15:00-15:30

Advancing Microbiome Therapies: Innovations in Fecal Microbiota Transplantation Beyond

Clostridioides difficile Infection

Jung-Hwan Lee (Inha University Hospital)

S5-2 15:30-16:00

Development of a Synbiotic Agent Using a Novel High-throughput Screening System

Tae-Gyu Lim (Sejong University)

Chair: Hakdong Shin (Sejong University)

S5-3 16:00-16:30

From Probiotics to Postbiotics: Key to Functional Foods and Health

Sung Wook Hong (World Institute of Kimchi)

www.msk.or.kr | 15

S5-4 16:30-17:00

From FMT to Next Generation Probiotics: Current Strategies and Progress in Future NGP

Drug Development

Wonsuk Lee (BioBankHealing Corp.)

S6 Engineering Biology: The Dominant Approach to Engineer Biosystems for

Bioeconomy Oct 31 (Thu), Halla Hall

Chair: Byung-Kwan Cho (KAIST)

S6-1 09:00-09:20

Microbial Enzyme Application for RNA Engineering

Eung-Soo Kim (Inha University)

S6-2 09:20-09:40

Multiplex Transcriptional Characterizations across Diverse Bacterial Species Using Cell-free

Systems

Sung Sun Yim (KAIST)

S6-3 09:40-10:00

Systems and Synthetic Biology for Efficient Microbial Cell Factory Engineering

Hyun Gyu Lim (Inha University)

Chair: Dong-Woo Lee (Yonsei University)

S6-4 10:00-10:20

Development of Highly Efficient C1 Gas-utilizing Microorganism Based on Synthetic Biology

Sangrak Jin (Yeungnam University)

S6-5 10:20-10:35

Systems and Synthetic Biology Approaches to Engineer Gut Commensal for Potential

Therapeutic Applications

Kangsan Kim (KAIST)

16 | www.msk.or.kr

S6-6 10:35-10:50

A Strategic Blueprint for the Domestication of Geobacillus stearothermophilus as a

Thermophilic Platform Cell Using the DNMB Suite

Jae-Yoon Sung (Yonsei University)

S7 From Shadow to Petri Dish: Breakthroughs in Culturing Elusive Microorganisms

Oct 31 (Thu), Samda Hall

Co-organized by Korean Collection for Type Cultures

Chair: Song-Gun Kim (KRIBB)

S7-1 09:00-09:30

Culturing Novel Microbes Relevant to Environment: Nitrifiers and Methanotrophs

Sung-Keun Rhee (Chungbuk National University)

S7-2 09:30-10:00

Microbial Dark Matter Mining: Challenges and Opportunities

Wen-Jun Li (Sun Yat-Sen University, P. R. China)

S7-3 10:00-10:30

How Culture Collections Diversify the Microbial Resources

Takashi Itoh (RIKEN BioResource Research Center, Japan)

S7-4 10:30-11:00

Exploration of Chemolithotrophs and Thermophiles from Marine Environments; An Omnibus

Story

Kae Kyoung Kwon (KIOST)

www.msk.or.kr | 17

S8 Development of Vaccines against New and Variant Infectious Diseases

Oct 31 (Thu), Rm 303

Co-organized by Professional Training Program for Infectious Disease Research (MIST)

Chair: In Seop Kim (Hannam University)

S8-1 09:00-09:05

Introducing the “Open Industrial Workforce Retraining Program for Infectious Disease

Response”

In Seop Kim (Hannam University)

S8-2 09:05-09:35

Korea Advanced Vaccine Development Center (KAVAD) Roles in Pandemic Preparedness

Jae-Hwan Lim (Korea Advanced Vaccine Development Center / Andong National University)

S8-3 09:35-10:05

Development of Innovative Vaccine and Safe Therapeutic by mRNA Technology

Woo Ghil Lee (RNAGENE Inc.)

S8-4 10:05-10:30

Success Factors for Rapid Development of mRNA Vaccine against Diverse Infectious Disease

within 100 Days

Joo-Sung Yang (ST PHARM)

S8-5 10:30-11:00

Strategy to Develop Updated COVID-19 Vaccines Based on Ad5/35 Platform for Effective

Response to Emerging Variants

Chang-Yuil Kang (Cellid Co., Ltd.)

18 | www.msk.or.kr

S9 Agricultural and Livestock Industrialization Based Microbiome

Oct 31 (Thu), Rm 302

Sponsored by 농림축산식품부/농림식품기술기획평가원/농생명마이크로바이옴혁신기술기반구축사업 &

과학기술정보통신부/한국연구재단/다부처 국가생명연구자원 선진화사업

Chair: Pyoung Il Kim (CIALM)

S9-1 09:00-09:30

Changes in Microbiota Composition Due to Crop Rotation of Tomatoes and Rice

Hyun Gi Kong (Chungbuk National University)

S9-2 09:30-10:00

Unraveling the Role of Seed Endophytes in Shaping Tomato Health

Kihyuck Choi (Dong-A University)

S9-3 10:00-10:30

Animal Gut Microbiome Bank (AGMB)

Ju Huck Lee (KRIBB)

S9-4 10:30-11:00

The Effects of Selected Synbiotics on Gut Microbiota, and Short-chain Fatty Acid Production

of Weaned Pigs

Yangseon Kim (Center for Industrialization of Agriculture and Livestock Microorganisms)

S10 Microbiome

Oct 31 (Thu), Halla Hall

Chair: Hana Yi (Korea University)

S10-1 13:00-13:15

Effect of Enterotype-dependent Stability of the Gut Microbiome on Post-gastrectomy

Malnutrition in Gastric Cancer Patients

Han Na Oh (Ajou University Hospital)

www.msk.or.kr | 19

S10-2 13:15-13:50

Discovery and Annotation of Proteins from Microbiome

Baolei Jia (Xianghu Laboratory, P. R. China)

S10-3 13:50-14:25

Multipartite Parasitic Interactions in the Aquatic Food Web: Modulating Gene Exchange and

Carbon Flow

Maliheh Mershad (Swedish University of Agricultural Sciences, Sweden)

S10-4_Keynote Speaker 14:25-15:00

Gut Microbial Strain Resources and Potential Beneficials to Host Health

Shuang-Jiang Liu (Shandong University, P. R. China)

S11 Engineering of Biocatalysts: Enzymes and Cells _ Keynote Session

Oct 31 (Thu), Samda Hall

Sponsored by the Next-generation Biorefinery Platform Technologies Development Program

(NRF, MSIT)

Chair: Bong Hyun Sung (KRIBB)

S11-1 13:00-13:30

Simulations to Understand Enzyme Evolution and Drug Resistance

Adrian J. Mulholland (University of Bristol, UK)

S11-2 13:30-14:00

Protein Design of the Stevioside Converting Enzyme for Activity and Stability

Eui-Jeon Woo (KRIBB)

S11-3 14:00-14:30

Engineering Perspectives on Methanotrophs for Methane Bioconversion

Eun Yeol Lee (Kyung Hee University)

20 | www.msk.or.kr

S11-4 14:30-15:00

Discovery of New Biocatalyst for Hydrocarbon-based Plastics Biodegradation

Soo-Jin Yeom (Chonnam National University)

S12 Exploring Basic Mechanisms and Applications in Viral Immunology, Vaccines, and

Therapeutics Oct 31 (Thu), Rm 303

Chair: Yong Seok Jeong (Kyung Hee University) & Jeong Yoon Lee (Jeonbuk National University)

S12-1 13:00-13:30

Physiological Functions of RIG-I Like Receptors

Takashi Fujita (Kyoto University, Japan)

S12-2 13:30-13:55

A Rationally Designed Live Attenuated Vaccine Induces Strong Mucosal Responses and

Complete Protection against SARS-CoV-2

Jinjong Myoung (Jeonbuk National University)

S12-3 13:55-14:20

A Novel Approach for Controlling Pulmonary Diseases by Targeting the Newly Identified

Lung-specific Immunoreceptor

Ji-Seung Yoo (Kyungpook National University)

S12-4 14:20-14:45

High-dimensional Analysis of Virus-specific T Cell Responses by Mass Cytometry

Heeju Ryu (Sungkyunkwan University)

S12-5 14:45-15:00

A Humanized ACE2 Mouse Model Recapitulating Age- and Sex-dependent

Immunopathogenesis of COVID-19

Uni Park (Seoul National University College of Medicine)

www.msk.or.kr | 21

S13 Fungal Genetics and Biology

Oct 31 (Thu), Rm 302

Chair: Kyung-Tae Lee (Jeonbuk National University)

S13-1 13:00-13:30

Molecular Features and Intelligent Modeling for Classification of Fungal Lifestyles

Jaeyoung Choi (Kyung Hee University)

S13-2 13:30-14:00

Discovery of Intrinsic and Extrinsic Drug Resistance Principles through Clinical Fungal

Isolates

Kyung-Tae Lee (Jeonbuk National University)

S13-3 14:00-14:30

Factors Influencing the Nitrogen-source Dependent Flucytosine Resistance in Cryptococcus

Species

Dong-Hoon Yang (Yonsei University MIRAE Campus)

S13-4 14:30-14:50

Identification of Essential Genes for the Establishment of Spray-induced Gene Silencing

(SIGS)-based Disease Control in Fusarium graminearum

Sieun Kim (National Institute of Horticultural & Herbal Science)

S14 Unravelling the Relationship between the Gut Microbiome and Skin Health:

Gut-Skin-Immune Axis Oct 31 (Thu), Rm 301

Sponsored by Research Collaboration Center for K-Beauty

Chair: Ju-Hoon Lee (Seoul National University) & Hyeun Bum Kim (Dankook University)

S14-1 13:00-13:30

Extracellular Vesicles of Limosilactobacillus fermentum SLAM216 Ameliorate Skin

Symptoms of Atopic Dermatitis by Regulating Gut Microbiome on Serotonin Metabolism

Younghoon Kim (Seoul National University)

22 | www.msk.or.kr

S14-2 13:30-14:00

Probiotics as Supplemental Preventive and Therapeutic Agents against Primary

Dysmenorrhea

Hyeun Bum Kim (Dankook University)

S14-3 14:00-14:30

Characterization of Exopolysaccharide Produced by Probiotics and Its Protective Effects on

Skin Damage

Nam Su Oh (Korea University)

S14-4 14:30-15:00

Integrating Metabolomics Analysis in Synbiotics Research: Unveiling Postbiotics’ Role in Skin

Wrinkle Reduction

Hakdong Shin (Sejong University)

S15 Environmental Microbiology

Oct 31 (Thu), Halla Hall

Chair: Woojun Park (Korea University)

S15-1_Keynote Speaker 16:00-16:30

Unexpected Implications and Applications of Methanotrophy

Jeremy D. Semrau (University of Michigan, USA)

S15-2 16:30-17:00

Raman-based Identification and Isolation of Microorganisms of Interest in Complex Microbial

Communities

Kang Soo Lee (UNIST)

S15-3_Keynote Speaker 17:00-17:30

Bacterial Quantal Secretion through Membrane Vesicles

Toyofuku Masanori (University of Tsukuba, Japan)

www.msk.or.kr | 23

S15-4 17:30-18:00

Polymyxin B-Induced Alteration in Bacterial Cell Membranes

Woojun Park (Korea University)

S16 Structure and Function of Microbial Proteins

Oct 31 (Thu), Samda Hall

Chair: Jinki Yeom (Seoul National University)

S16-1_Keynote Speaker 16:00-16:30

Do Plasmids Pick a Bacterial Partner before Committing to Conjugation?

Gad Frankel (Imperial College London, UK)

S16-2 16:30-17:00

Managing the Number of Specialized Ribosomes

Yoon-Mo Yang (Hanyang University)

S16-3 17:00-17:30

Activation of Zinc Uptake Regulator by Zinc Binding to Three Regulatory Sites

Junseock Koh (Seoul National University)

S16-4 17:30-17:45

An Archaeal Transcription Factor EnfR with a Novel ‘Eighth Note’ Fold Controls Hydrogen

Production of a Hyperthermophilic Archaeon Thermococcus onnurineus NA1

Da-Woon Bae (Ewha Womans University)

S16-5 17:45-18:00

PhoPQ-Mediated Modification of the Lipopolysaccharide Inner Core Affects Intrinsic

Resistance to Tetracycline Antibiotics and Bile Salts in Escherichia coli

Umji Choi (Myoungji University)

24 | www.msk.or.kr

S17 Innovative Approaches to Microbiome-Human Interactions Using Next Generation

Sequencing Oct 31 (Thu), Rm 303

Sponsored by Sanigen Co., Ltd

Chair: Hakdong Shin (Sejong University)

S17-1 16:00-16:30

Inflammation-mediated Microbiota Killing Drives Aspartate-dependent Salmonella

Typhimurium Expansion in the Inflamed Gut

Woongjae Yoo (POSTECH)

S17-2 16:30-17:00

RBC Interplay in Pathogen-induced Thrombosis

Han Young Chung (Chungnam National University)

Chair: Bokyung Son (Dong-A University)

S17-3 17:00-17:30

Molecular Mechanisms of the Intestinal Epithelium in Regulating

Nutrient-Microbiota-Immune Interactions

Ye-Ji Bang (Seoul National University)

S17-4 17:30-18:00

DATA Driven Foodborne Pathogen Detection Using NGS Technology and Its Modification

Jinho Choi (Sanigen Co., Ltd)

S18 IVI session - Advances in Vaccine Technology

Oct 31 (Thu), Rm 302

Sponsored by International Vaccine Institute

Chair: Manki Song (IVI)

S18-1 16:00-16:30

Salmonella Typhi Vi-Specific Serological Profiling among Children, Adolescent, and Adults

after Typhoid Vaccination

Jae Seung Yang (International Vaccine Institute)

www.msk.or.kr | 25

S18-2 16:30-17:00

Development of Immune Modulating Ionizable Lipids and Their LNP Formulation for mRNA

Vaccines and Therapeutics

Hyukjin Lee (Ewha Womans University)

Chair: Jae-Ouk Kim (IVI)

S18-3 17:00-17:30

Advancing Vaccine Delivery: Microneedle Array Patches for Enhanced mRNA Vaccine

Stability and Efficacy

Jooyoung Kim (QuadMedicine, Inc.)

S18-4 17:30-18:00

Development of 4-Valent Vaccine Conferring Optimal Protection against Pore-forming

Toxins of Staphylococcus aureus

Gi-Sub Choi (CLIPS BnC Co.)

S19 Bio-Biz Festival

Oct 31 (Thu), Rm 301

Chair: Seung-Hwan Park (KRIBB)

S19-1 16:00-16:30

Microbiome Therapeutics: The “Molecule-First” Strategy for Targeted Interventions

Sang Sun Yoon (Yonsei University/BioMe Inc.)

S19-2 16:30-17:00

Resolving Biology with Scalable, Single Cell Multiomic Solutions

YuJeong Gho (DAON BioSciences Inc.)

S19-3 17:00-17:30

Comprehensive Bioinformatics Solution for Microbial Genomics, Metagenomics

Kyung-yun Kim (Insilicogen, Inc.)

26 | www.msk.or.kr

S19-4 17:30-18:00

The Career Path of Microbiologists to Being a Corporate Researcher

Byung-Yong Kim (CKD Healthcare)

S20 Microbial Ecology

Nov 1 (Fri), Halla Hall

Chair: Kae Kyoung Kwon (KIOST)

S20-1 09:00-09:30

Biofilm Control by Metazoa in Membrane Systems for Wastewater Treatment

Seonki Lee (National Korea Maritime & Ocean University)

S20-2 09:30-10:00

Assessment of Ecological Disturbance through Phenotypic Dynamics of Groundwater

Microbiome

Jin-Kyung Hong (Chonnam National University)

S20-3 10:00-10:30

Isolation of Novel Archaeal Virus Infecting Marine Ammonia-oxidizing Thaumarchaea

Jong-Geol Kim (Wonkwang University)

S20-4 10:30-11:00

Application of WGS for Source Tracking for Antibiotic Resistance (STAR)

Tatsuya Unno (Chungbuk National University)

www.msk.or.kr | 27

S21 Recent Advances in the Molecular Biology of Microbes in Host Environments

Nov 1 (Fri), Samda Hall

Chair: Eun-Jin Lee (Korea University)

S21-1 09:00-09:25

The Role of Histone Modifications in Regulating Responses to External Signals in Candida

albicans

Jung-Shin Lee (Kangwon National University)

S21-2 09:25-09:50

Dynamic 3D Genome Reorganization in Response to Environmental Stress

Kyoung-Dong Kim (Chung-Ang University)

S21-3 09:50-10:15

The Formylmethionine Ribosome Quality Control Pathway in Saccharomyces cerevisiae for

Cold Adaptation

Cheol-Sang Hwang (Korea University)

S21-4 10:15-10:40

MARTX Toxin, the Primary Virulence Factor Produced in Vibrio vulnificus

Byoung Sik Kim (Ewha Womans University)

S21-5 10:40-10:55

A Salmonella Type II TA Module Leads to Formation of Persister Cells inside Host

Soomin Choi (Korea University)

28 | www.msk.or.kr

Graduate Students’ Forum

GS1 Graduate Students’ Forum 1 Oct 30 (Wed), Halla Hall

Chair: Minsik Kim (Yonsei University) & Ju Huck Lee (KRIBB)

GS1-1 12:00-12:08

Enhancing Gut Health: The Impact of Seed Mucilage Prebiotics on Infant and Adult

Microbiomes

Kanika Mahra (Kyungpook National University)

(C082)

GS1-2 12:08-12:16

Gut Microbiome Modulation by Cellulose-degrading Bacteria for Enhancing Gut Health and

Growth Performance in Mouse Model

Gi Beom Keum (Dankook University)

GS1-3 12:16-12:24

Polyhydroxyalkanoate Production in Halophilic Marine Bacteria and Assessing Bioplastics

Biodegradability

Sondor Ganbat (Silla University)

(G056)

GS1-4 12:24-12:32

Metagenomic Profiling of Age-related Changes in Facial Skin Microbiome

Ikwhan Kim (Kyungpook National University)

(C081)

GS1-5 12:32-12:40

Gut Microbiome in Patients with Parkinson’s Disease Varies by Premotor Rapid-eye

Movement Sleep Behavior Disorders Status

Jae-Yun Lee (Kyung Hee University)

GS1-6 12:40-12:48

Epigallocatechin-3-gallate Ameliorates Inflammatory Bowel Disease through Gut

Microbiota-dependent Modulation of NLRP6 Inflammasome Signaling

Soo-Jeong Lee (Seoul National University)

www.msk.or.kr | 29

GS1-7 12:48-12:56

CRISPR-guided Platforms for Functional Research and Engineering of Pseudomonas

aeruginosa Phages

Shin-Yae Choi (CHA University)

GS1-8 12:56-13:04

Evaluation of Potential Probiotics Candidates with Anti-allergic Effect to Alleviate Mast Cell

Degranulation and Food Allergy Symptoms in Balb/c Mouse Model

So-Yeon Jin (Seoul National University)

GS1-9 13:04-13:12

Halotolerant Beneficial Bacteria Alleviate Salinity Stress in Maize by Modulating Soil

Microbiome

Tino Bashizi (Kyungpook National University)

(B143)

GS1-10 13:12-13:20

Understanding the Influence of a Foliicolous Lichen on the Endophytic Mycobiome Using a

Metabarcoding Approach

YunHyeok Jang (Changwon National University)

GS1-11 13:20-13:28

Gut Microbiome–Creatine Synergistic Effects against Obesity

Su-Won Jeong (Kyung Hee University)

(C080)

GS2 Graduate Students’ Forum 2 Oct 30 (Wed), Samda Hall

Chair: Hyunjin Yoon (Ajou University) & Jeong Yoon Lee (Jeonbuk National University)

GS2-1 12:00-12:08

Identification and Validation of Small Molecule Inhibitor Targeting Biofilm Formation

Regulator CsgD

Yunjeong Kim (Ewha Womans University)

(D083)

GS2-2 12:08-12:16

Reassessing the Diversity of Penicillium in Marine Environments with the Proposal of Twelve

New Species

Ji Seon Kim (Seoul National University)

30 | www.msk.or.kr

GS2-3 12:16-12:24

Accumulated Peptidoglycan Turnover Products Lead to Excessive Outer Membrane Vesicle

Production in Salmonella Typhimurium 14028S

Jihye Yang (Korea University)

(D082)

GS2-4 12:24-12:32

The Potential of C-di-AMP as a Druggable Target for Reversing β-Lactam Resistance in

MRSA

Ji-Hoon Kim (Sungkyunkwan University)

GS2-5 12:32-12:40

The Impact of Gut Microbiome on Type 2 Diabetes Improvement: Insights from Roux-en-Y

Reconstruction after Gastrectomy

GyuDae Lee (Kyungpook National University)

(C084)

GS2-6 12:40-12:48

Modulation of Gut Microbiota and Metabolites by Laminarin during In Vitro Human Fecal

Fermentation: Bacteroides as a Potential Key Decomposer

Juhui Jang (Sejong University)

GS2-7 12:48-12:56

Sweet Food, Bitter Life: Glucose-mediated Acidic Stress Compromises the Membrane

Integrity in Clinical Acinetobacter baumannii

Jihyeon Min (Korea University)

(B142)

GS2-8 12:56-13:04

A Periplasmic Protein Modulates the Proteolysis of Peptidoglycan Hydrolases to Maintain

Cell Wall Homeostasis in Escherichia coli

Sohee Park (Sungkyunkwan University)

GS2-9 13:04-13:12

Optimal Selection of Bacteria-mediated Delivery to Enhance Therapeutic Efficacy in Breast

Cancer Models

Jam-Eon Park (KRIBB)

(C079)

www.msk.or.kr | 31

GS2-10 13:12-13:20

Characterization of a Plasmid-dependent Phage for Controlling Antibiotic Resistance

Gene-carrying Bacteria in the Gut Microbiota

Geunsoo Jung (Sejong University)

GS2-11 13:20-13:28

Interplay Between Vaginal Microbiome and High-risk HPV in Cervical Dysplasia

Da-Ryung Jung (Kyungpook National University)

(C083)

GS2-12 13:28-13:36

Fructose-responsive Regulation by FruR in Faecalibacterium prausnitzii for Its Intestinal

Colonization

HyeLim Choi (Seoul National University)

GS3 Graduate Students’ Forum 3 Oct 30 (Wed), Rm 303

Chair: Donghyuk Kim (UNIST) & Yoonjee Chang (Kookmin University)

GS3-1 12:00-12:08

Therapeutic Potential of Engineered Endolysin CD27L_EAD against Clostridioides difficile

Infection

Youngjin Cho (Ajou University)

GS3-2 12:08-12:16

Investigation of Inflammation Alleviation in an IBD Mouse Model Using the

Butyrate-producing Strain and Tributyrin

Hyuna Sung (Yonsei University)

GS3-3 12:16-12:24

Metagenomic, Untargeted Metabolomic, and Physicochemical Insights of Korean Fermented

Foods

Amani Sliti (Kyungpook National University)

32 | www.msk.or.kr

GS3-4 12:24-12:32

Developing Oral Vaccines against Foot-and-Mouth Disease Virus Using Saccharomyces

cerevisiae

Le My Tieu Ngoc (Jeonbuk National University)

(G054)

GS3-5 12:32-12:40

Potential Role of malR-malABC Regulon in Inducing Virulence of Staphylococcus

pseudintermedius during Canine Otitis Externa

Ji Hyun Lim (Seoul National University)

GS3-6 12:40-12:48

Computational Analysis of E4 Gene Evolution in Human Adenovirus Species D

Chanhee Lee (Jeonbuk National University)

GS3-7 12:48-12:56

Endolysin PHICD111_20024_EAD as a Potent Therapeutic Agent against Clostridioides

difficile Infection

Jeongseok Park (Ajou University)

GS3-8 12:56-13:04

Genome Editing with Small Prime Editors in Bacteria

Seong Hyeok Ma (Yonsei University)

GS3-9 13:04-13:12

Induced Dysbiosis to Resident Soil Microbial Communities Improves Plant Health by Enabling

Exotic Microbial Agents to Prevail in the Rhizosphere

Yohannes Ebaubye Andargie (Kyungpook National University)

GS3-10 13:12-13:20

The Diversity and Functional Characterization of Indigenous Bradyrhizobium Isolates in

Korea

Hyorim Choi (Rural Development Administration)

www.msk.or.kr | 33

GS3-11 13:20-13:28

Structural Investigation of the Docking Domain Assembly from trans-AT Polyketide

Synthases

Se-Young Son (Ewha Womans University)

GS3-12 13:28-13:36

Replicating the Human Gut: An In Vitro Co-culture System for Host and Anaerobic Microbial

Interaction

Han Eol Yang (KRIBB)

(D014)

Graduate Students’ Forum 4 Nov 1 (Fri), Rm 303

Co-organized by BET Research Institute, Chung-Ang University & Institute of

Microbiomics, Chung-Ang University

Chair: Keun Pil Kim (Chung-Ang University) & Jae-Ouk Kim (IVI)

GS4-1 09:00-09:08

Control of Lysogeny Formation in Escherichia coli Using CRISPR-Cas12f1-Armed Synthetic

λ Bacteriophages

Ho Joung Lee (Chung-Ang University)

(G055)

GS4-2 09:08-09:16

Anti-prion System in Saccharomyces cerevisiae: Innate Immunity to Inside-the-Cell Risk

Sia Han (Pusan National University)

GS4-3 09:16-09:24

Comparison of Microbial Diversity Applied to Amplicon Sequencing Variant and

High-throughput Cultivation in Lakes of Maritime Antarctica

Jiyoung Yoon (Korea Polar Research Institute)

GS4-4 09:24-09:32

Mannitol Metabolism as a Key Factor in the Pathogenicity of Vibrio vulnificus Biotype 1C

Joon-gi Kwon (Seoul National University)

34 | www.msk.or.kr

GS4-5 09:32-09:40

Selenium Nanoparticles Synthesized by Lactobacillus plantarum as a Gram-positive

Selective Bactericidal Agent

Gyeong-Min Kim (Pusan National University)

(D084)

GS4-6 09:40-09:48

Microbial Precise Genome Editing Using Truncated sgRNA and Paired Cas9-NG Nickase

Song Hee Jeong (Chung-Ang University)

(G053)

GS4-7 09:48-09:56

Pangenome Analysis-based Real-Time PCR for Rapid Quantification of 19 Live Probiotics in

Real Food

Yunjeong Kim (Seoul National University)

GS4-8 09:56-10:04

Three Ribosomal Subunit Proteins as Candidates for Anti-prion Components in

Saccharomyces cerevisiae

Min Suk Jang (Pusan National University)

GS4-9 10:04-10:12

A Method Evaluation for Analyzing Microbial Communities in Ice Cores of Styx Glacier,

Antarctica

Subin Lee (Korea Polar Research Institute)

GS4-10 10:12-10:20

Gain-of-Function Mutation in the Galactitol-specific GatC Transporter Facilitates Glucose

Uptake in E. coli

Suon Jeong (Chung-Ang University)

(G052)

GS4-11 10:20-10:28

Molecular Identification of Cryptococcus Isolated from Korean Patients and Discovery of

Drug Resistance Mechanism

Eui-Seong Kim (Jeonbuk National University)

Plenary Lectures

PL3

Supported by Korea Research Institute of Bioscience and Biotechnology (KRIBB)

2024 International Meeting of the Microbiological Society of Korea

36 | www.msk.or.kr

PL1

Treating Human Diseases with Bugs and Not Drugs

Michael J. Sadowsky

1Department of Medicine, 2BioTechnology Institute,

3Department of Soil, Water & Climate, University of Minnesota, USA

Over the last 15 years, microbial transplantation therapeutics (MTT) have been applied to

treat a variety of human diseases. Perhaps the best example of this is with recurrent Clostridium

difficile infection (rCDI) causing failure of the distal gut organ. Thus far, we have treated 1000’s

of patients and we have used 16S rRNA and PacBio gene sequencing, metagenomics, and

metabolomics to understand the microbial ecology and bacterial composition of colonic

microflora before and after treatment by MTT. By 14 days post-transplantation, the fecal

bacterial composition of the recipients is highly similar to that of the donor and results in

patient cure, suggesting that the donor’s bacteria quickly occupied their requisite niches and

established functioning microbial communities. To overcome barriers in our clinical MMT

program we developed frozen, freeze-dried, and drinkable MTT preparations without loss of

apparent efficacy in clearing recurrent CDI – the overall success rate was 89%. We now better

understand the mechanism(s) allowing engraftment of fecal microbiota and curing of C. difficile

disease. More recently, this technology has proven to be useful to treat a variety of diseases

affecting gut microbiota, including autism spectrum disorder, Pitt Hopkins disease, and irritable

bowel syndrome. This innovative technology holds great promise for treating even a greater

number of patients presenting a variety of diseases – without drugs!

www.msk.or.kr | 37

Plenary Lectures

PL2

The Mouth and the Gut - Together in Sickness and in Health

Nobuhiko Kamada1,2,3

1Division of Gastroenterology and Hepatology, Department of Internal Medicine,

2Department of Pathology, University of Michigan, USA

3Laboratory of Microbiology and Immunology, Immunology Frontier Research Center (IFReC),

Osaka University, Japan

Mucosal tissues in various anatomic locations develop site-specific microbial and immune

ecosystems. Despite the high specialization of both the mucosal immune system and the

resident microbial community in each organ, recent findings have revealed communication

between different mucosal organs. We recently demonstrated microbial and immunological

connections between the oral and gut mucosae. During oral mucosal inflammation, specific oral

pathobionts abnormally expand in the oral cavity. Certain oral pathobionts can produce

antimicrobial molecules that inhibit the growth of competing oral symbionts, leading to their

dominance. The accumulated oral pathobionts are ingested and translocated to the lower

digestive organs. Under healthy gut conditions, the resident gut microbiota resists ectopic

colonization by oral microbes. However, in disease states such as gut dysbiosis or colitis,

translocated oral microbes can colonize the gut mucosa and contribute to the pathogenesis of

gastrointestinal diseases, including inflammatory bowel disease and colorectal cancer. Notably,

oral pathobionts utilize distinct adaptation mechanisms for gut colonization compared to their

native niche, the oral mucosa. Therefore, oral pathobionts employ sophisticated strategies to

maximize fitness in different mucosal sites, collectively contributing to the pathogenic

connections between the oral and gut mucosae.

[This work was supported by the National Institute of Health, USA]

2024 International Meeting of the Microbiological Society of Korea

38 | www.msk.or.kr

PL3

Investigating the First Principles of Cell Biology Using a

Minimal Bacterial Cell

John Glass

J. Craig Venter Institute, USA

The minimal cell is the hydrogen atom of cellular biology. Such a cell, because of its simplicity

and absence of redundancy would be a platform for investigating just what biological

components are required for life, and how those parts work together to make a living cell. Since

the late 1990s, our team at the Venter Institute has been developing a suite of synthetic

biology tools that enabled us to build what previously has only been imagined, a minimal cell.

Specifically, a bacterial cell with a genome that expresses only the minimum set of genes

needed for the cell to divide every two hours that can be grown in pure culture. That minimal

cell has about half of the genes that are in the bacterium on which it was based, Mycoplasma

mycoides JCVI syn1.0, the so-called synthetic bacteria we reported on in 2010. We used

transposon bombardment to identify non-essential genes, and genes needed to maintain rapid

growth in M. mycoides. Those findings required re-design and re-synthesis of some reduced

genome segments. Three cycles of design, synthesis, and testing, with retention of quasi‐

essential genes, produced synthetic bacterium JCVI‐Syn3.0 (531 kb, 474 genes), which has a

genome smaller than that of any autonomously replicating cell found in nature. Synthetic

bacterium JCVI-Syn3.0 retains almost all genes involved in synthesis and processing of

macromolecules. Surprisingly, it also contained 149 genes with unknown biological functions,

suggesting the presence of undiscovered functions essential for life. This minimal cell is a

versatile platform for investigating the core functions of life, and for exploring whole‐genome

design. Since it was initially reported in 2016, we have identified functions for about 70 of the

original 149 genes of unknown function. These findings have been used to create flux balance

analysis and kinetic whole cell computational models of our minimal cell that replicate

laboratory observations about our minimal cell. Additionally, we have used JCVI-syn3.0, which

has an abnormal cell division and cell morphology phenotype, and a JCVI-syn3.0 mutant

containing an additional seven non-essential genes that has divides normally and looks like wild

type Mycoplasma mycoides to investigate how modern cell division and cell size control might

have evolved.

www.msk.or.kr | 39

Plenary Lectures

PL4

Structure-guided Drug Discovery Targeting Cell Wall

Antibiotic Resistance Mechanisms in Staphylococcus aureus

Natalie C.J. Strynadka

Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada

Broad-spectrum β-lactam antimicrobial (AMR) resistance in Staphylococcus aureus is a

significant global healthcare and economic burden. In clinical strains, resistance is largely

controlled by BlaR1, a receptor that senses β-lactams through the acylation of its sensor

domain, inducing transmembrane signalling and activation of the cytoplasmic-facing

metalloprotease domain. The BlaR1 metalloprotease domain has a role in BlaI derepression,

inducing expression of blaZ (serine β-lactamase PC1) and mecA (β-lactam-resistant cell-wall

transpeptidase PBP2a). Both these latter enzymes are major AMR resistance mechanisms which

have been intensive targets for the structure-guided development of inhibitors that act in

concert with the classical β-lactam antibiotics. As the master controller of their expression, we

show that BlaR1 cleaves BlaI repressor directly, as necessary for inactivation. Cryo-electron

microscopy structures of BlaR1, wild type and autocleavage-deficient mutants, suggest that

allosteric signalling emanates from β-lactam-induced exclusion of the prominent extracellular

loop bound competitively in the sensor-domain active site, driving subsequent dynamic

motions, including a shift in the sensor towards the membrane and accompanying changes in

the zinc metalloprotease domain. We propose that this enhances expulsion of autocleaved

products from the active site, shifting the equilibrium to a state permissive of efficient BlaI

cleavage and ensuing expression of PC1 and PBP2a. Collectively, this analysis provides atomic

insight into a novel two-component signalling receptor that mediates action—in this case, multi

pronged broad spectrum β-lactam antibiotic resistance—through direct cleavage of a repressor

as well as for structure-guided design of therapeutics that block its action.

[This work was supported by the Canadian Institutes of Health Research]

MSK Award Lecture

2024 International Meeting of the Microbiological Society of Korea

42 | www.msk.or.kr

Oh My Goodness Microbe! -

My Journey to Cultivate the Uncultured

Jang-Cheon Cho

Department of Biological Sciences and Bioengineering, Inha University

We are in the midst of a remarkable era for microbiology. The rapid expansion of genomic

diversity studies on Earth’s microbes, including the human microbiome, has illuminated the

immense significance of microbial life. However, despite the wealth of genomic data, we often

find ourselves in a “data-rich but information-poor” phase, where unlocking the full potential of

multi-omics research requires more than just bioinformatics. It demands deep insights into the

microbes themselves and the ability to link their genetic makeup to their physiological roles. A

key to this understanding lies in microbial cultivation. Since 2000, I have embarked on a mission

to cultivate the uncultured, employing high-throughput culturing methods based on

dilution-to-extinction techniques to uncover abundant yet uncharacterized microorganisms,

particularly in oligotrophic aquatic environments. This journey has led to the discovery and

formal naming of at least one new phylum, two classes, two orders, eight families, 32 genera,

and 92 species. Many more organisms, some representing new phyla and classes, are under

study in my lab, opening exciting avenues for genomic and physiological exploration. In our lab,

we have successfully isolated and characterized dominant microorganisms from marine

environments, including SAR11, SAR202, SAR86, SAR116, SAR92, OM60, OM43, and OMG, as

well as prominent freshwater bacteria such as acI, acIV, and LD28. Our recent work has focused

on understanding metabolic dependencies, like heme auxotrophy, to explain why many

microorganisms remain uncultured. Despite these breakthroughs, many crucial clades from

deep-sea environments, including SAR11 IIb and SAR324, remain elusive and await further

study. In this presentation, I will share my ongoing journey to “cultivate the uncultured,”

highlighting the joys and challenges these enigmatic microbes continue to offer. I will reflect on

how these discoveries, along with our work on “Oh My Goodness Microbe,” have been a source

of inspiration and scientific excitement.

Luncheon Symposium

2024 International Meeting of the Microbiological Society of Korea

44 | www.msk.or.kr

LS1

Introduction to Shotgun Metagenome Analysis and

Reporting

Taewook Kim

CJ Bioscience, Inc. (NGS Sales team, BI Analysis)

This lecture provides a comprehensive overview of microbiome analysis and shotgun

metagenomics, focusing on fundamental concepts and the latest research trends. We will

explore why shotgun metagenomics is used for microbiome analysis, how it differs from

amplicon metagenomic sequencing, its advantages, and the amount of data needed to obtain

significant results. We will delve into shotgun metagenomics as a powerful method for analyzing

microbial communities.

Additionally, we will introduce the UBCG-based shotgun metagenomic analysis approach

developed by CJ Bioscience. We will examine how it differs from known methods, its accuracy

through comparisons with actual data, and showcase methodologies for metagenomic data

extraction and interpretation. These insights will be illustrated through real-world case studies,

demonstrating how shotgun metagenomics is applied to discover new microbial functions,

study disease-related microbial communities, and develop new therapies.

Finally, the lecture will cover the key steps in the downstream analysis of shotgun

metagenomic data, including taxonomic classification, functional annotation, and pathway

reconstruction. We will discuss the importance of these analyses in extracting biological

meaning and interpreting complex microbial interactions.

www.msk.or.kr | 45

Luncheon Symposium

LS2

The Latest Next-generation Sequencing Innovations

Kyoungwoo Jang

Illumina Korea

In this seminar you will hear from Illumina experts focusing on MiSeq i100 with smaller scale

applications, XLEAP-SBS™ chemistry and DRAGEN™ analysis. You will learn about fast and

simple sequencing workflows available to you with new, innovative Illumina solutions.

2024 International Meeting of the Microbiological Society of Korea

46 | www.msk.or.kr

LS3

Introduction of Advanced Microbiome Analysis Software

Platform CosmosID-HUB

Seong Min Baek

Clinical Microbiomics

CosmosID-HUB is an advanced microbiome analysis software platform designed to streamline

and enhance the process of genetic sequencing data evaluation through various bioinformatics

pipelines.

This sophisticated software supports the analysis of 16S, ITS, shotgun metagenomic, and

shotgun metatranscriptomic data, allowing users to upload sequencing data to the cloud which

is then rapidly profiled. Leveraging AWS infrastructure, the processing can be completed within

a few minutes to several hours, depending on the number of samples.

The software platform offers a highly intuitive interface where clients can effortlessly create

virtual cohorts using study metadata, which is both uploadable and editable directly from the

HUB dashboard.

These cohorts can be used for comprehensive comparative microbiome analyses, initiated

through a user-friendly process of defining the comparative analysis, selecting metadata for

comparison, and organizing cohorts by order and selecting a specific colour.

This process is further enhanced with customizable workflows tailored to specific research

needs.

Upon completion of the comparative analysis, CosmosID-HUB provides an array of

interactive, dynamic charts, and visualizations, alongside detailed statistical analyses including:

∙Dynamic tabular results

∙Configurable heatmap and stacked bar charts with high-resolution PDF exports

∙Abundance Distribution Analysis with Wilcoxon Rank-Sum Testing

∙2D & 3D PCA

∙Alpha Diversity with Wilcoxon Rank-Sum Testing

∙Beta Diversity with PERMANOVA testing

∙Read Statistics

∙LEfSe Analysis

Moreover, the results can be exported in various formats, suitable for inclusion in

publications, presentations, and reports, thus facilitating scientific communication and

documentation. This makes CosmosID-HUB an indispensable tool for researchers aiming to

conduct detailed and efficient microbiome studies.

Symposia [S1]

2024 International Meeting of the Microbiological Society of Korea

48 | www.msk.or.kr

S1-1

Akkermansia muciniphila-Derived Amuc_1409 Protein

Improves Gut Health through Intestinal Stem Cell

Regulation

Eun-Jung Kang1,2, Young-Eun Kim2, Yong-Hoon Kim2, Dukjin Kang3, and Chul-Ho Lee1*

1Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology

2Department of Veterinary Pathology, College of Veterinary Medicine, Chungnam University

3Group of Biometrology, Korea Research Institute of Standards and Science

Akkermansia muciniphila, one of the most abundant members of the gut microbiota, is a

promising next-generation probiotic agent owing to its role in regulating host health. A.

muciniphila has been reported to have its beneficial roles in gut health by regulating gut

immunity, promoting intestinal epithelial development, and improving barrier integrity.

However, A. muciniphila-derived functional molecules regulating gut health are not well

understood. Recently, we have identified and characterized the biological function of

Amuc_1409, a previously uncharacterized A. muciniphila-secreted protein. Amuc_1409 have

shown the increased intestinal stem cell (ISC) proliferation and regeneration in ex vivo intestinal

organoids and in vivo models of radiation- or chemotherapeutic drug-induced intestinal injury

and natural aging mice. Mechanistically, Amuc_1409 promoted E-cadherin/β-catenin complex

dissociation via interaction with E-cadherin, resulting in the activation of Wnt/β-catenin

signaling. Our findings suggest that A. muciniphila secreted Amuc_1409 acts as a novel

regulatory mediator of ISC homeostasis and is a promising biomolecule for improving and

maintaining gut health.

www.msk.or.kr | 49

Symposia [S1] : Microbiome Interactions and Health Impacts: From Gut to Skin

S1-2

Applications of the Human Gut Microbiome Information in

Koreans: Towards Precision Nutrition and Healthcare

Eun-Ji Song, Ji-Hee Shin, and Young-Do Nam*

Personalized Diet Research Group, Korea Food Research Institute

Understanding the human gut microbiome is crucial for precision nutrition and healthcare.

Traditional amplicon analysis has limitations in resolving complex microbial interactions and

functional potential. To address this, we used advanced applications with Korean gut

microbiome data. First, we used a hybrid assembly approach, combining short and long read

sequencing, to construct Metagenome-Assembled Genomes (MAGs). We linked 16S rRNA gene

information from these MAGs with the Amplicon Sequence Variants (ASVs) from Korean gut

microbiome amplicon data. This provides a detailed understanding of microbial functions and

their contributions to host health. Second, we integrated the Plant-based Diet Index (PDI) and

gut microbiome data into a machine learning model to predict metabolic syndrome. This model

reveals the interplay between diet and gut microbiota, suggesting precise diet modulation

informed by microbiome profiles can manage and prevent metabolic disorders in Koreans. These

strategies highlight the potential of microbiome-based precision nutrition and healthcare for

tailored interventions to improve health outcomes.

2024 International Meeting of the Microbiological Society of Korea

50 | www.msk.or.kr

S1-3

Skin Microbe-dependent TSLP-ILC2 Priming Axis in

Early Life is Co-opted in Allergic Inflammation

Jimin Cha1†, Tae-Gyun Kim1,2†, and Ji-Hwan Ryu1*

1Department of Biomedical Sciences, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei

University College of Medicine

2Department of Dermatology, Severance Hospital, Cutaneous Biology Research Institute, Yonsei

University College of Medicine

†These authors contributed equally to this work

Although early life colonization of commensal microbes contributes to long-lasting immune

imprinting in host tissues, little is known regarding the pathophysiological consequences of

postnatal microbial tuning of cutaneous immunity. Here, we show that postnatal exposure to

specific skin commensal Staphylococcus lentus (S. lentus) promotes the extent of atopic

dermatitis (AD)-like inflammation in adults through priming of group 2 innate lymphoid cells

(ILC2s). Early postnatal skin is dynamically populated by discrete subset of primed ILC2s driven

by microbiota-dependent induction of thymic stromal lymphopoietin (TSLP) in keratinocytes.

Specifically, the indole-3-aldehyde-producing tryptophan metabolic pathway, shared across

Staphylococcus species, is involved in TSLP-mediated ILC2 priming. Furthermore, we

demonstrate a critical contribution of the early postnatal S. lentus-TSLP-ILC2 priming axis in

facilitating AD-like inflammation that is not replicated by later microbial exposure. Thus, our

findings highlight the fundamental role of time-dependent neonatal microbial-skin crosstalk in

shaping the threshold of innate type 2 immunity co-opted in adulthood.

www.msk.or.kr | 51

Symposia [S1] : Microbiome Interactions and Health Impacts: From Gut to Skin

S1-4

Gut Commensal Microbe Regulated by Host Imd Signaling

Pathway Accelerates Adult Emergence in Female Fruit Fly

Hyun Sik Kim1,2, Tae Woong Whon3, Jae-Yun Lee2, and Jin-Woo Bae1,2*

1Department of Biology, Kyung Hee University

2Department of Life and Nanopharmaceutical Sciences, Kyung Hee University

3Kimchi Functionality Research Group, World Institute of Kimchi

One example of sexual dimorphism in animals is protogyny, where female development

occurs earlier than in males. Protogyny is common phenomenon observed in a wide range of

mammals, including humans. However, the role of the gut microbiome in the rapid sexual

development in females remains poorly understood. The present study aims to investigate the

inter-relationships between flies and their resident gut microbiota. We show that Acetobacter

oryzifermentans, a commensal bacterium of Drosophila, induces protogyny by shortening the

developmental time from pupa to adult in females. We identify that the abundance of A.

oryzifermentans is sex-specific and controlled by the Imd signaling pathway. A.

oryzifermentans, which is abundant in the female gut, interacts with the host to metabolize

dietary phytol to pristanic acid, and pristanic acid ultimately promotes host development.

These observations provide compelling evidence that the accelerated development commonly

observed in female animals is the result of differences in the gut microbiome.

Symposia [S2]

2024 International Meeting of the Microbiological Society of Korea

54 | www.msk.or.kr

S2-1

Deciphering the Bacterial-Fungal Crosstalk in the

Gut Microbiota with Chemical Biology Tools

Lanxin Li and Yuan Qiao*

School of Chemistry, Chemical Engineering and Biotechnology (CCEB),

Nanyang Technological University (NTU), Singapore

Candida albicans is the major opportunistic fungal pathogen in humans. Normally residing in

the host gut niche as a benign yeast state, C. albicans can transform into long filamentous

hyphae that facilitate its mucosal penetration and bloodstream dissemination, causing deadly

infections in the host. Among the numerous signals that trigger C. albicans hyphal growth,

bacterial peptidoglycan fragments (PGNs) represent the most potent inducers. Thus,

addressing the mechanistic details of PGN-induced C. albicans invasive growth may lead to

novel insights to combat this deadly fungus.

In this talk, I will present our recent series of studies on gut microbiota-derived PGNs in

triggering C. albicans hyphal growth. We developed versatile chemoenzymatic PGN probes with

photoaffinity, bio-orthogonal, or fluorescent functionality to enable studies of PGN recognition

in C. albicans. Recently, we uncovered that the surface sensing and cellular uptake of PGNs

represent two distinct cellular processes in C. albicans, addressing long-sought-after questions

in the PGN-induced C. albicans hyphal growth. These insights provide new opportunities to

thwart C. albicans hyphal growth.

www.msk.or.kr | 55

Symposia [S2] : Host-pathogen Crosstalk: From Molecule to Physiology

S2-2

Exploring Host-pathogen Interactions: Unmasking a

Salmonella Genotoxin’s Assault on Mitochondria

Shu-Jung Chang

Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan

Bacterial virulence factors are potent tools that manipulate host physiology to facilitate

bacterial invasion. Among these, bacterial genotoxins stand out by directly attacking

chromosomal DNA, leading to cell damage and death. However, the underlying molecular

mechanisms of their virulence remain unclear. In this symposium, we will unveil a novel function

of typhoid toxin, a genotoxin produced by Salmonella Typhi, which also targets host

mitochondria, a critical signaling hub during infection. This targeting strategy reveals the

physiological impact and molecular mechanisms through which bacterial pathogens exploit

mitochondria to enhance their virulence.

[This work was supported by Taiwan National Science and Technology Council and a Taiwan

Ministry of Education]

2024 International Meeting of the Microbiological Society of Korea

56 | www.msk.or.kr

S2-3

Unravelling of Complex Poxvirus Structures Using

Cryo-electron Microscopy

Yeontae Jang, Seungmi Kim, and Jaekyung Hyun*

School of Pharmacy, Sungkyunkwan University

Assembly of mature and infectious poxvirus requires multi-step process that involves

coordination of viral proteins. Morphological descriptions of poxvirus particles at various stages

of virion assembly largely owed to transmission electron microscopy of isolated virions or

ultra-thin sections of cells infected with viruses. However, detailed molecular mechanism

behind maturation process and the exact morphology of the virions remained enigmatic.

Thanks to recent technical breakthroughs in cryo-electron microscopy (cryo-EM), complex

poxvirus structure is being studied in molecular detail. Using single particle cryo-EM and

electron tomography, we show that scaffold protein of vaccinia virus (VACV), prototypal

poxvirus, self-assemble to govern the initiation and formation of immature poxvirus particles.

The VACV scaffold protein D13 was recombinantly expressed and purified and assembled in

vitro into particles that mimic authentic scaffold layer of immature virus particles. Detailed

cryo-EM structures of assembly products reveal that the N-terminal α-helix of D13 plays a

pivotal role in the regulation and initiation of scaffold assembly. Given the critical role in viral

replication cycle, and highly level of conservation across poxvirus genera, the scaffold protein is

proposed to be a novel antiviral target of poxvirus diseases.

www.msk.or.kr | 57

Symposia [S2] : Host-pathogen Crosstalk: From Molecule to Physiology

S2-4

Multi-omics Approaches to Enhance Metabolic Activities of

Microorganisms

Jungyeon Kim

Graduate School of International Agricultural Technology, Seoul National University

As the use of genome-edited microorganisms rapidly expands, enhancing health benefits

through genetically manipulated probiotics is receiving great attention. Among the various

strategies to build probiotics with enhanced health benefits, an approach that enhances the

intestinal metabolic activity of probiotics can be the answer. Saccharomyces boulardii and

Escherichia coli strain Nissle 1917 are probiotic microorganisms that exhibits therapeutic

effects on common gastrointestinal diseases. Although they have been clinically used for

decades to treat gastrointestinal diseases, several studies have reported weak or no beneficial

effects in some cases. Multi-omics approaches have revealed metabolic bottlenecks in their

metabolism of mucosal glycan components, suggesting insufficient intestinal metabolic activity

of probiotics. To enhance metabolic activity in the intestine, the probiotic strains were

genetically engineered to efficiently metabolize L-fucose or D-galactose, the main components

of mucosal glycans. Additionally, the engineered probiotic strains were orally administered to

mice to validate their enhanced intestinal metabolic activity. The engineered probiotic strains

secreted more recombinant proteins in the cecum and colon, validating enhanced intestinal

metabolic activity.

2024 International Meeting of the Microbiological Society of Korea

58 | www.msk.or.kr

S2-5

Intracellular and Intra-organ Host Environments Differentially

Shape the Survival Fitness of Staphylococcus aureus

JuOae Chang and Wonsik Lee*

School of Pharmacy, Sungkyunkwan University

Ubiquitous pathogen methicillin-resistant Staphylococcus aureus (MRSA) harbors ability to

adapt and thrive in various host environments adding to the challenge in combating this

pathogen. However, a comprehensive analysis of genetic mechanisms involved in such ability

remains unexplored. In this study, we employed transposon sequencing (Tn-seq) in in vitro

intracellular infection and in vivo murine sepsis models to analyze adaptive strategies of MRSA

in different host environments. We have identified the unique and common genetic

determinants essential for MRSA survival in these different host niches. Moreover, with the

murine sepsis model, we have shown that each organ imposes unique growth constraints on

bacteria. As a result, different organs foster heterogeneity within the mutant population able to

enter and survive in each organ. In sum, with these results we not only provide better and

comprehensive understanding of bacterial physiology in the host environments but also

suggest possibly effective therapeutic targets for treatment of S. aureus infection.

Symposia [S3]

Co-organized by Science Research Center

for Microbial Survival Systems

2024 International Meeting of the Microbiological Society of Korea

60 | www.msk.or.kr

S3-1

AmiC Modulates RNase E-Mediated Decay of the Small RNA

SgrS during Glucose Phosphate Stress in Escherichia coli

Eunkyoung Shin1, Yubin Yang2, Jungyeop Kim2, Hana Hyeon2,

Yuhyun Jang2, Minju Joo2, and Kangseok Lee2*

1Department of Microbiology, Catholic University of Daegu School of Medicine

2Department of Life Science, Chung-Ang University

A previous study has suggested that AmiC, which is known as the N-acetylmuramoyl-L-

alanine amidase, can modulate the endoribonucleolytic activity of RNase E in Escherichia coli.

AmiC enhanced the multimerization levels of RNase E, resulting in increased cleavage activity on

long RNA substrates exceeding approximately 100 nucleotides. In this study, we identified SgrS,

a small regulatory RNA involved in stress response to cellular accumulation of phosphorylated

sugars, as an specific in vivo target of AmiC-mediated RNase E activity. Our findings suggest

that AmiC-mediated modulation of RNase E cleavage activity adds another layer of gene

expression regulation during the recovery from sugar phosphate stress in E. coli.

www.msk.or.kr | 61

Symposia [S3] : Microbial Survival Systems

S3-2

An Extracellular Lipoprotein Released from OMV Enhances

Biofilm Maturation via Specific Interaction with

Exopolysaccharides

Bo-Ram Jang, Kyung-Jo Lee, Hui-wan Yang, and Kyu-Ho Lee*

Molecular Microbiology Laboratory, Department Life Science, Sogang University

IlpA, a lipoprotein anchoring to the outer membrane (OM) of Vibrio vulnificus is a multifaceted

virulence factor that facilitates bacterial cytoadherence to host cells and induces cytokine

production in the host. In the present study, an additional role of IlpA has been investigated in

the developmental process for biofilm maturation. An ilpA-deletion mutant was not able to

reach a stage constructing the mature biofilms. This defect of the mutant was relieved by

exogenous supplementation of the recombinant polypeptides including the central

OM-anchoring domain of IlpA. Since the N- and C-terminal regions of the cellular IlpA are facing

toward the periplasmic space, a secretion system is required for the OM-anchoring domain of

IlpA to be exposed to the extracellular milieu. IlpA secretion was achieved through the process

for the outer membrane vesicle (OMV) production. IlpA embedded in OMVs was freed by the

activity of at least two distinct phospholipases either within the OMVs or in the OMV-free

culture supernatants. To understand the mechanism for IlpA to enhance biofilm maturation, its

interaction abilities with the major components of biofilm matrix were further investigated. The

two types of exopolysaccharides (EPS) constituting the most abundant polysaccharides in

biofilm matrix, which are known as essential factors for biofilm maturation by V. vulnificus, were

specifically bound by the central OM-anchoring domain of IlpA. Therefore, it is proposed that

the extracellular IlpA, of which secretion and exposure are mediated by OMVs and

phospholipases, respectively, plays an important role in biofilm maturation via specific

interaction with EPS.

2024 International Meeting of the Microbiological Society of Korea

62 | www.msk.or.kr

S3-3

Evolutionary Unique N-Glycan-dependent Glycoprotein

Quality Control System Is Critical for Cellular Fitness and

Extracellular Vesicle Transport in Cryptococcus neoformans

Catia Mota1, Ye Ji Son1, Kiseung Kim1, Eun Jung Thak1, Su-Bin Lee1,

Jeong-Kee Yoon2, Min-Ho Kang3, Heeyoun Hwang4, Yong-Sun Bahn5,

J. Andrew Alspaugh6, and Hyun Ah Kang1*

1Department of Life Science, College of Natural Science, Chung-Ang University

2Department of Systems Biotechnology, Chung-Ang University

3Department of Biomedical-Chemical Engineering, The Catholic University of Korea

4Korea Basic Science Institute

5Department of Biotechnology, College of Life Science of Biotechnology, Yonsei University

6Department of Medicine, Duke University, Durham, North Carolina, USA

Eukaryotes evolved a conserved N-glycan-dependent endoplasmic reticulum protein quality

control (ERQC) system to ensure the accurate folding of glycoproteins. The human pathogen

Cryptococcus neoformans has a unique N-glycosylation pathway lacking glucose addition step

to the core N-glycan precursors in the ER but carrying additional basidiomycetes-specific

mannosidases. To investigate the molecular features and functions of the ERQC system in C.

neoformans, we characterized a set of mutants with deletion of genes coding for the ERQC

sensor UDP-glucose: glycoprotein glucosyltransferase (UGGT), and putative α1,2-mannose

trimming enzymes (MNS1A and MNS1B). The uggtΔ, mns1AΔ, mns1BΔ, and mns1AΔmns1BΔ

mutants showed alterations in the N-glycan profiles, defective cell surface organization,

decreased survival within host cells, and loss of in vivo virulence with different extents. The

uggtΔ strain exhibited severe impairment particularly in extracellular secretion of capsule and

virulence-related enzymes. Comparative transcriptome revealed the upregulation of genes

related to protein folding, proteolysis, and cell wall remodeling, indicating induced ER stress in

uggtΔ, whereas no apparent changes in the expression of genes involved in protein secretion

or capsule biosynthesis. The analysis of extracellular vesicles (EVs), combined with proteome

analysis, revealed notable alterations in the cargo loading, number and size distribution of EVs

in uggtΔ. Our findings emphasize the requirement of functional ERQC for cellular fitness under

adverse conditions and for proper EV-mediated transport of virulence bags, which are crucial

for full fungal pathogenicity.

www.msk.or.kr | 63

Symposia [S3] : Microbial Survival Systems

S3-4

Direct Interaction of RPA with Rad52 Regulate

Meiotic Recombination

Jeong Hwan Joo1#, Soogil Hong1#, Mika T Higashide2,3, Eui-Hwan Choi1,4, Seobin Yoon1,

Min-Su Lee1, Hyun Ah Kang1, Akira Shinohara2, Nancy Kleckner5, and Keun Pil Kim1*

1Department of Life Sciences, Ching-Ang University

2Institute of Protein Research, Graduate School of Science, Osaka University, Japan

3Current address: Graduate School of Engineering, Fukui University, Japan

4New Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation

5Department of Molecular and Cellular Biology, Harvard University, USA

#The first two authors should be regarded as Joint First Authors

During meiosis, programmed double-strand breaks (DSBs) are generated by Spo11, and

resection of these DSBs were generated 3’ single stranded DNA (ssDNA) tails by exonuclease.

One DSB end engages a homolog partner chromatid and is extended by DNA synthesis, whereas

the other end remains associated with its sister. Then after regulated differentiation into

crossover- and noncrossover-fated types, the second DSB end participates in the reaction by

strand annealing with the extended first end in both cases. This process, known as second-end

capture, depends on Rad52 which presumably facilitates this through its known ability to

anneal two ssDNA. Our physical analysis of DNA recombination reveals that second-end capture

depend on direct interaction of Rad52 with the ssDNA binding protein, replication protein A

(RPA). We found that the absence of Rad52-RPA joint activity leads to a

cytologically-prominent RPA spike, which emerges from the homolog axes at crossover sites

during the pachytene stage of meiotic prophase. This spike likely represents the DSB end of a

broken chromatid, either due to the displaced leading DSB end or the second DSB end which has

unable to engage with the homolog-associated ssDNA. These findings suggest a close

correspondence between the roles of Rad52 and RPA in meiotic recombination and mitotic DSB

repair.

2024 International Meeting of the Microbiological Society of Korea

64 | www.msk.or.kr

S3-5

Elucidation of Core Bacterial Communities and Their Metabolic

Characteristics in the Phycosphere of Marine Algae

Jeong Min Kim, Byeong Jun Choi, Hülya Bayburt, and Che Ok Jeon*

Department of Life Science, Chung-Ang University

Marine algae along the Korean Peninsula harbor diverse symbiotic microbial communities that

play crucial roles in the health and functioning of their algal hosts. Despite their importance, the

core bacterial members and their symbiotic functions remain underexplored. This study aims to

elucidate the core bacteria associated with marine algae through comprehensive community

analysis and to highlight their novel symbiotic functions. A total of 43 samples of Rhodophyta,

Phaeophyta, and Chlorophyta were collected from the southern, western, and eastern coasts of

Korea. The microbiome of the algal sphere was categorized into loosely attached environment

(LAE) and tightly attached environment (TAE), and the bacterial communities in the surrounding

seawater were also analyzed. Beta diversity analysis revealed distinct bacterial communities

based on sampling regions and attachment levels (LAE and TAE), suggesting significant

environmental differentiation driven by specific bacterial species. Genes associated with

potential symbiotic interactions were significantly more abundant in LAE and TAE compared to

seawater, indicating that bacterial taxa in these environments are better equipped for

symbiotic interactions necessary for survival in the high-density cell environment of the algal

sphere. Indicator value (IndVal) analysis identified Psychromonas and Marinomonas as core taxa

in LAE, Litorimonas and Vibrio in TAE, and Pseudoalteromonas in both LAE and TAE.

Bioinformatic analysis revealed that these key taxa formed hub nodes in the network, showing

multiple positive or negative correlations with other bacterial taxa. Additionally, key taxa

distinguishing LAE and/or TAE communities from seawater communities were associated with

metabolic features related to marine algal interactions, such as bacterial chemotaxis, flagellar

assembly, two-component systems, biofilm formation, and siderophore biosynthesis,

supporting their role as keystone species in algal sphere interactions. This study highlights the

dynamic symbiotic functions and interactions of core bacteria with marine algae, contributing

to our understanding of the role of symbionts in the adaptability and resilience of marine algae

in changing environments.

www.msk.or.kr | 65

Symposia [S3] : Microbial Survival Systems

S3-6

The Role of KIF18A in Antiviral Immune Responses

Younghyun Lim, Yong-Bin Cho, and Young-Jin Seo*

Department of Life Science, Chung-Ang University

Kinesin family member 18A (KIF18A) plays a crucial role in various cellular processes, including

motility, cell division, and microtubule dynamics. However, its role in antiviral immune responses

remains largely unexplored. In this study, we identified KIF18A as a key regulator of antiviral

immunity. Specifically, inhibition of KIF18A significantly enhanced the ability of dendritic cells

(DCs) to activate CD8+ T cells. RNA sequencing analysis revealed that KIF18A inhibition leads to

significant alterations in genes associated with antigen degradation in DCs. Consistent with

this, KIF18A inhibition reduced the antigen degradation capacity of DCs, resulting in increased

surface expression of antigen-MHC-I complexes. Furthermore, KIF18A directly influences CD8+

T cell function. The KIF18A inhibitor treatment or KIF18A knockdown significantly enhanced

CD8+ T cell function. Notably, administration of a KIF18A inhibitor markedly reduced morbidity

and mortality of influenza virus-infected mice. In conclusion, our findings suggest that targeting

KIF18A inhibition may offer a promising therapeutic strategy to enhance antiviral immune

responses.

Symposia [S4]

Sponsored by the National Research Foundation of

Korea (NRF) grant, funded by the Korean government (MSIT)

(RS-2024-00342735)

2024 International Meeting of the Microbiological Society of Korea

68 | www.msk.or.kr

S4-1

Grand Challenges in Phage and Endolysin Therapy

Sangryeol Ryu

Department of Food and Animal Biotechnology, Seoul National University

The use of bacteriophages and their lytic enzymes to control various pathogenic bacteria has

emerged as a promising new tool to solve the antibiotic resistance crisis. Bacteriophages are

viruses that specifically infect and lyse bacterial cells by taking over metabolic machineries of

the host in lytic cycle. Phages are ubiquitous in environments and have a long history of safe

use. In contrast to antibiotics, phages do not disturb normal microbial flora because of its highly

specific host range. Two different approaches such as phage cocktail and phage engineering to

overcome bacterial resistance to phage infection and narrow host range of the phage will be

discussed as a base to maximize the efficiency of phage therapy. Endolysins, or lysins, are

phage-encoded muralytic enzymes that directly cleavage bonds in the peptidoglycan of the

bacterial cell wall. As endolysins can quickly lyse the Gram-positive bacteria when they are

applied exogenously, they are considered as a promising enzyme-based antibacterial agent.

However, most endolysins are not active against the Gram-negative bacteria because they

cannot penetrate the outer membrane barrier of the Gram-negative bacteria. Numerous

antimicrobial peptides derived from phage genomes and their fusion proteins with endolysins

were tested for their antimicrobial activity against the Gram-negative bacteria. Several

promising peptides were found but their in vivo activity is inconsistent, suggesting that new

approaches are required for developing phage-derived antimicrobial peptides active against the

Gram-negative bacteria.

www.msk.or.kr | 69

Symposia [S4] : Present and Future of Bacteriophage Research

S4-2

Defense Mechanisms against RNA Phages in

Pseudomonas aeruginosa

Hee-Won Bae, Shin-Yae Choi, Hyeong-Jun Ki, and You-Hee Cho*

Program of Biopharmaceutical Science, College of Pharmacy and Institute of Pharmaceutical Sciences,

CHA University

Bacterial mechanisms that antagonize the phage lifecycle stages have been characterized as

the components in antiphage defense systems that have been evolved during the arms race

between phages and bacteria. The antiphage defense systems work basically at the five steps of

the phage lifecycle, which include phage adsorption, genome penetration, nucleic acid and

protein synthesis, virion assembly, and phage release. One such example is adsorption

prevention, which includes loss of phage receptors from hosts and the acquisition of physical

barriers hiding receptors. Another example can be seen during host restriction occurring after

phage adsorption. This includes blockage in genome penetration, superinfection immunity,

restriction modification, and CRISPR systems. The arsenal of host systems to resist phages may

explain the host tropism of phages, but most of the research has been done for DNA phages,

rather than RNA phages. Considering the potential significance and ecological diversity of RNA

phages as comparable as those of RNA viruses in higher eukaryotes, we first analyzed the host

tropism of the small RNA phage, PP7 that infects Pseudomonas aeruginosa PAO1, but neither

PAK nor PA14, among the most widely used laboratory strains. We found that a set of group II

pilins are required for PP7 infection and suggested the molecular details of the phage-receptor

interaction. We also revealed that other host factors might govern the host tropism of PP7 in

P. aeruginosa clinical strains. Topics discussed will include the rational and principle to identify

the defense system genes against RNA phages. This is based on our reverse genetic system of

the RNA phages that can bypass their genome entry limited by receptor specificity. Genetic

identification of the host factors that restrict the PP7 lifecycle stages such as genome

penetration and phage release will be covered as well.

2024 International Meeting of the Microbiological Society of Korea

70 | www.msk.or.kr

S4-3

Phage-based Strategies for Targeting Refractory

Bacterial Infections

Longzhu Cui

Division of Bacteriology, Department of Infection and Immunology, School of Medicine,

Jichi Medical University, Tochigi, Japan

The rise of antibiotic-resistant bacteria has created an urgent need for innovative

antibacterial agents. Phage therapy, which utilizes bacteriophages to target bacterial

infections, is emerging as a promising alternative. In our previous work, we developed a

phage-based approach for sequence-specific bacterial killing, capable of selectively eliminating

drug-resistant bacteria. By engineering non-replicative phages and incorporating specific

genes, we have made progress in enhancing phage safety, targeting, and stability.

In this presentation, I will share insights from our ongoing study, which aims to contribute to

the advancement of antibacterial therapy through phage engineering. Our current focus is on

generating phage-based drugs and refining gene incorporation techniques to tailor phages for

specific therapeutic applications. For drug-resistant bacteria, we are working on phages that

may counteract resistance mechanisms, potentially offering a targeted and effective

alternative to conventional antibiotics. In the case of biofilm-forming bacteria, our research is

exploring phages with improved biofilm-disruption capabilities, addressing one of the major

challenges in treating persistent infections. Additionally, we are investigating synthetic phages

designed to access and control intracellular bacteria, with the hope of providing solutions for

infections that have traditionally been difficult to treat.

Moreover, our research extends into phage-based vaccine development, where we are

exploring the use of phages as delivery vehicles for immunogenic components, which could

represent a novel approach to preventing bacterial infections. Through these multifaceted

strategies, we aspire to contribute to the establishment of phages as a versatile tool in the fight

against refractory bacterial infections, offering new possibilities for conditions that have

resisted existing treatments.

[This research was funded by AMED (JP24ae0121045, JP24gm1610002), JSPS KAKENHI

(JP24H00662), and partially by Moonshot R&D Program for Agriculture, Forestry, Fisheries

(JPJ009237).]

www.msk.or.kr | 71

Symposia [S4] : Present and Future of Bacteriophage Research

S4-4

CRISPR-Cas System:

From Genome Editing to Bacterial Attack

Ho Joung Lee, Song Hee Jeong, and Sang Jun Lee*

Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University

The CRISPR-Cas system was first identified as a microbial immune defense mechanism,

comprising two core components. The first is the guide RNA, which recognizes specific

nucleotide sequences. The second is the Cas nuclease, which cleaves the phosphodiester bonds

of the recognized target nucleic acid. Due to its modularity, the CRISPR-Cas system has been

adapted into a highly versatile genome-editing tool. By altering the sequence of the guide RNA,

this system can target and cleave any desired nucleotide sequence with precision.

Our research team has modified guide RNAs in various CRISPR-Cas systems, including

CRISPR-Cas9 and Cas12a, to edit bacterial genomes and bacteriophage genomes at the

single-nucleotide level. We have also constructed synthetic bacteriophages embedded with

multiple CRISPR-Cas systems. These CRISPR-loaded synthetic phages successfully infect host

bacteria, recognizing and cleaving specific genotypes within host cells. This system also

effectively prevents the emergence of lysogenic cells harboring the targeted genotypes. As a

result, we have developed a sequence-specific microbial control system.

In this presentation, the evolution of the CRISPR-Cas system will be discussed, tracing its

journey from its initial identification as a microbial immune mechanism, to its development as a

precise genome-editing tool, and finally, to its application in controlling target microorganisms.

2024 International Meeting of the Microbiological Society of Korea

72 | www.msk.or.kr

S4-5

Innovative Strategies for Enhancing the Efficacy of

Bacteriophage and Endolysin

Jaewoo Bai

Department of Food Science and Technology, Seoul Women’s University

Bacteriophages (phages) and endolysins present promising potential as natural antibacterial

agents for controlling foodborne pathogens. However, the practical application of phages is

hindered by the rapid development of bacterial resistance to phage infection. In case of

endolysin, the effectiveness of exogenously applied recombinant endolysins is limited to

Gram-positive bacteria due to their inability to penetrate the outer membrane of

Gram-negative bacteria. In addition, the challenges of endolysin purification and stability during

storage further complicate their application. To address these issues, novel strategies have

been developed and integrated into food systems. First, a phage cocktail comprising three

distinct phages targeting different host receptors was formulated. Second, a liposome-

mediated endolysin encapsulation system was designed and evaluated for its ability to

penetrate the outer membrane of Gram-negative bacteria. Finally, a yeast-based expression

system was employed for continuous endolysin production. These strategies demonstrate the

potential of phages and endolysins as novel antimicrobial agents for controlling foodborne

pathogens within food systems.

Symposia [S5]

Sponsored by BioBankHealing Corp.

2024 International Meeting of the Microbiological Society of Korea

74 | www.msk.or.kr

S5-1

Advancing Microbiome Therapies: Innovations in

Fecal Microbiota Transplantation Beyond

Clostridioides difficile Infection

Jung-Hwan Lee

Microbiome Center, Department of Hospital Medicine, Inha University Hospital, Inha University School of

Medicine

Fecal Microbiota Transplantation (FMT) has long been recognized as an effective treatment

for Clostridioides difficile (C. difficile) infection, but recent advancements are expanding its

potential to treat a wide array of health conditions. This presentation explores the

cutting-edge innovations in FMT and microbiome therapies that go beyond the traditional C.

difficile applications. Highlighting the pioneering research conducted at Inha University

Hospital’s Microbiome Center, we will delve into the evolving role of FMT in treating

inflammatory bowel disease, multi-drug resistant organisms, and even neurological conditions

such as cognitive dysfunction.

We will also discuss the ongoing research aimed at enhancing FMT’s efficacy, which promises

to streamline and scale the therapy. Through a review of clinical trials and published studies, this

session will provide a comprehensive understanding of how FMT is being integrated into

next-generation probiotics and its potential to revolutionize healthcare. We will gain insights

into the broader applications of microbiome-based therapies and the future of precision

medicine in managing complex diseases.

www.msk.or.kr | 75

Symposia [S5] : Probiotics 2.0: Exploring the Future of Next Generation Probiotics

S5-2

Development of a Synbiotic Agent Using a

Novel High-throughput Screening System

Heejin Ko, Ju-Hoon Lee, Hakdong Shin*, and Tae-Gyu Lim*

Department of Food Science & Biotechnology, Sejong University

Recent research has highlighted the intricate connection between the gut microbiome and

skin health, referred to as the gut-skin axis. Our research has focused on this link, leveraging a

novel high-throughput screening (HTS) system to identify potential microbial strains and

candidate materials with skin condition improving properties such as wrinkle reduction and skin

whitening. In this study, we selected two specific microbial strains and two natural extracts with

promising wrinkle reduction/skin whitening effects through HTS. To validate these findings, we

performed efficacy analysis using human keratinocyte HaCaT cells. The results demonstrated a

clear connection between the initial HTS screening outcomes and in vitro efficacy. Furthermore,

we examined the effects on 3D human skin tissue models, where we observed that the

identified strain Lacticaseibacillus rhamnosus SLrh014 and the extract 38A1 exhibited

outstanding efficacy in promoting skin whitening and reducing wrinkles. These findings suggest

that these microbial strains and extract could be promising candidates for future cosmetic

applications aimed at skin beauty enhancement. This ongoing research marks a significant step

toward understanding the gut-skin axis and opens new possibilities for the development of

nutricosmetic products.

2024 International Meeting of the Microbiological Society of Korea

76 | www.msk.or.kr

S5-3

From Probiotics to Postbiotics:

Key to Functional Foods and Health

Sung Wook Hong

Kimchi Functionality Research Group, World Institute of Kimchi

Kimchi, a traditional Korean fermented vegetable food, has also become popular in the

western world because of its unique taste and beneficial properties, which are derived from the

various raw materials and secondary metabolites of the fermentative microorganisms used

during production. The representative kimchi lactic acid bacteria (LAB) reported so far are

Lactobacillus sp., Lactococcus sp., Pediococcus sp., and Leuconostoc sp. LAB have several

health benefits by promoting a healthy balance of beneficial bacteria and modulating gut

microbiome; however, techno-functional limitations such as viability controls have hampered

their full potential applications in the functional foods. Therefore, the focus is gradually shifting

from probiotic bacteria towards metabolites derived probiotics, so-called postbiotics. Recently,

postbiotics have emerged as another group of beneficial compounds that can help improve a

health. They have been linked to a number of health benefits for the gut, the immune system,

and various other aspects of health. The probiotics market is continuously growing and

changing, and new probiotics are constantly being developed. Postbiotics are an alternative to

probiotics that can be used by high-risk people for whom probiotic use can be dangerous.

Postbiotic preparations and applications are diverse, and offer a safety advantage over

probiotics in that the composition of the functional material is clearly defined, allowing

determination of safe dosage. In the future, Korea will witness a market transformation from

probiotics to postbiotics. Postbiotics can quickly restore the intestinal microbiome balance,

which not only improves intestinal health but also may help solve the challenges of our aging

society, as people expect to live in good health for 100 years. Postbiotics are a promising

technology with the potential to lead the next-generation bio-industry in preparation for the

fourth industrial revolution.

www.msk.or.kr | 77

Symposia [S5] : Probiotics 2.0: Exploring the Future of Next Generation Probiotics

S5-4

From FMT to Next Generation Probiotics: Current

Strategies and Progress in Future NGP Drug Development

Wonsuk Lee

R&D Center, BioBankHealing Inc

The therapeutic potential of the human microbiome is driving innovations in fecal microbiota

transplantation (FMT) and Next Generation Probiotics (NGPs). Stool banks are essential for the

safe and effective use of FMT, maintaining stringent standards for donor selection, microbial

screening, and sample processing. Currently FDA-approved for recurrent Clostridioides difficile

infection, FMT is being investigated for various conditions, including inflammatory bowel

disease (IBD), irritable bowel syndrome (IBS), and metabolic disorders. Its manufacturing follows

strict protocols, including comprehensive donor screening to minimize pathogen transmission,

precise sample preparation, and preservation techniques like cryopreservation to retain

microbial diversity and ensure safety and efficacy. Meanwhile, NGPs are advancing precision

microbiome therapeutics by harnessing specific microbial strains for targeted functional

benefits, presenting promising advancements in treating complex diseases. Ongoing attention

to these developments is crucial for realizing their transformative potential in future

microbiome therapies.

Symposia [S6]

2024 International Meeting of the Microbiological Society of Korea

80 | www.msk.or.kr

S6-1

Microbial Enzyme Application for RNA Engineering

Su-Been Yang, Yeong-Chan Kim, Si-Sun Choi, and Eung-Soo Kim*

Department of of Biological Sciences and Bioengineering, Inha University

Nucleoside deoxyribosyltransferase (NDT) catalyzes the transglycosylation reaction of the 2′

-deoxyribonucleoside between purine and/or pyrimidine bases and has been widely used in the

synthesis of nucleoside analogs for various biotechnological purposes. While NDT demonstrates

high activity towards natural nucleoside as substrate, it exhibits low activity towards

nucleoside analogs such as 2’-fluoro-2’-deoxyuridine (2FDU). Engineering of NDT becomes

essential for its industrial application. In this study, we generated a mutant NDT library by

error-prone PCR mutagenesis. Through screening of the mutant libraries, we identified two

mutant genes with higher conversion rates from 2FDU to 2’-fluoro-2’-deoxyadenosine (2FDA)

in E. coli whole-cell conversions.

Circular RNA is an RNA molecule with a closed structure where the single strand’s 5’ and 3’

ends are connected. Circular RNA can be generated through the process of RNA circularization,

which connects the ends of linear RNA. Typically, a ribozyme method known as permuted

introns and exons (PIE) is used for RNA circularization in vitro. However, circular RNAs produced

using the PIE method may contain foreign exon sequences, and the efficiency of RNA

circularization is not high. In this study, we explored an innovative approach that leverages the

secondary structure of linear RNA and the application of specific enzymes to synthesize circular

RNA in vitro for targeted gene expression.

www.msk.or.kr | 81

Symposia [S6] : Engineering Biology: The Dominant Approach to Engineer Biosystems for Bioeconomy

S6-2

Multiplex Transcriptional Characterizations across Diverse

Bacterial Species Using Cell-free Systems

Sung Sun Yim1,2

1Department of Biological Sciences, KAIST

2Graduate School of Engineering Biology, KAIST

Precise tuning of gene expression levels is crucial for engineering predictably behaving

genetic circuits. Our current understanding of how regulatory sequences control gene

expression levels remains limited for most bacterial species. Cell-free expression systems

greatly simplify prototyping of genetic designs in vitro. However, the small number of

simultaneous measurements that can be made using fluorescence or color as readouts limits

the scale at which biological parts can be characterized. Here we devised a method to measure

expression levels from thousands of regulatory sequences in single cell-free reactions using

oligo library DNA synthesis and targeted deep sequencing of RNA and DNA. This multiplexed

approach was highly robust and corresponded well with in vivo measurements in E. coli. We

further applied this approach in active cell-free transcription systems developed from ten

diverse bacterial species, enabling comparison of sequence-function relationships across hosts

and predictive modeling of transcriptional activation. We anticipate that this multiplexed

approach using cell-free expression systems will expand the capacity for genetic circuit

prototyping in new bacterial chassis.

2024 International Meeting of the Microbiological Society of Korea

82 | www.msk.or.kr

S6-3

Systems and Synthetic Biology for Efficient Microbial Cell

Factory Engineering

Hyun Gyu Lim

Department of Biological Engineering, Inha University

Biorefineries aim to produce a broad spectrum of chemicals from abundant biomass. To

achieve this goal, it is crucial to be capable of engineering microorganisms based on

understanding. In this talk, I will introduce several approaches to redesign microbial hosts based

on systems biology and synthetic biology. In the first part, large-scale genomic and

transcriptomic analyses of the wildtype or evolved strains will be introduced.

Bioprocess-relevant phenotypes were improved by Adaptive Laboratory Evolution (ALE) and

mutational mechanisms were investigated via whole-genome sequencing. Furthermore, its

transcriptional regulatory network was unveiled by analyzing hundreds of its transcriptome

profiles collected under various culture conditions. In the second part, I will introduce

synthetic-biology-driven engineering of bacterial hosts and a few examples of developing cell

factories converting low-cost biomass into value-added compounds. As a conclusion, the

combined synthetic biology and systems biology approaches can effectively facilitate host

engineering, which is critical for developing efficient bioprocesses.

www.msk.or.kr | 83

Symposia [S6] : Engineering Biology: The Dominant Approach to Engineer Biosystems for Bioeconomy

S6-4

Development of Highly Efficient C1 Gas-utilizing

Microorganism Based on Synthetic Biology

Sangrak Jin1,2,3 and Byung-Kwan Cho1,2,4*

1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

2KI for the BioCentury, Korea Advanced Institute of Science and Technology

3Department of Biotechnology, Yeungnam University

4Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology

Acetogenic bacteria, also known as acetogens, have garnered attention as promising

biocatalysts due to their Wood-Ljungdahl (WL) pathway, which enables them to utilize both

CO2 and CO for carbon fixation. I employed synthetic biology strategies to engineer acetogenic

bacteria to develop a highly efficient C1-to-C4 bioconversion system. First, to overcome the

growth inhibition about high concentrations of CO gas, I obtained CO-resistant strains through

adaptive evolution. These strains exhibited enhanced tolerance to high levels of CO gas and

improved the fixation efficiency of C1 gases. The platform strains (ECO2) obtained showed

more than 8-fold faster growth rates under high CO gas conditions than wild-type strains.

Second, I constructed a synthetic metabolic pathway using synthetic bio-parts and CO-sensing

module to produce 2,3-butanediol (2,3-BDO) by the CO-tolerant microorganisms under C1 gas

conditions. However, in the fermentation of C1 gases, the engineered strain exhibited lactate as

the primary product under a CO mixotrophic condition, although the yield of 2,3-BDO was only

around 30%. Finally, to solve this problem, I used CRISPR/Cas technology to delete the lactate

pathway in the ECO2 strain to address this. As a result, the ECO2 ldh KO strain no longer

produced lactate under mixotrophic conditions. When introducing the 2,3-BDO pathway into

the engineered strain, I observed a significant increase in the yield of 2,3-BDO, approximately

2.3 times higher compared to the results obtained with the wild-type strain expressing the

same 2,3-BDO pathway. These research findings are expected to contribute to a better

understanding of acetogenic microorganisms in biotechnology and enhance their industrial

applications.

2024 International Meeting of the Microbiological Society of Korea

84 | www.msk.or.kr

S6-5

Systems and Synthetic Biology Approaches to Engineer Gut

Commensal for Potential Therapeutic Applications

Kangsan Kim1,2, Yongjae Lee1,2, and Byung-Kwan Cho1,2,3*

1Department of Biological Sciences, Korea Advanced Institute of Science and Technology

2KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology

3Graduate School of Engineering Biology, Korea Advanced Institute of Science and Technology

Paralleled with the advances in systems and synthetic biology, the past decade has witnessed

a growing interest in engineering microbes as live bacterial therapeutics. In this endeavor, native

gut residential species are emergingly considered as host strains, owing to their innate capacity

to survive and stably propagate in the intestinal milieu for an extended duration. In this regard,

expanding our comprehension on the biology of gut commensals and the development of

advanced genetic toolbox will enable us to effective harness native gut commensals for

therapeutic and diagnostic applications. In this study, I employed systems and synthetic biology

approaches to design and engineer Bacteroides thetaiotaomicron, a prominent resident of the

human intestine, for potential therapeutic applications. First, the primary transcriptome

landscape of B. thetaiotaomicron was analyzed to elucidate regulatory elements involved in

gene transcription, discovering novel transcription start sites and cognate promoters in the

process. A functional nitrate-inducible promoter was also developed by pairing transcription

regulation of a native promoter under the control of a nitrate-sensing transcription factor,

allowing B. thetaiotaomicron to sense and respond to the biomarker of gut inflammation. Next,

a strain-specific genome-scale metabolic model (GEM) was reconstructed to better understand

its metabolic properties. Subsequently, GEM was consulted to guide metabolic engineering

design aimed at rewiring B. thetaiotaomicron to produce non-native butyric acid, a compound

with therapeutic relevance in gut inflammation. Lastly, through machine learning-based

analysis of transcriptomic data obtained under various culture conditions, we elucidated novel

transcriptional regulatory networks associated with stress response and in vivo survival. Taken

together, the comprehensive systems and synthetic biology approaches facilitated

understanding of metabolic and regulatory properties of B. thetaiotaomicron, and

demonstrated its potential utility as a candidate strain for future biotherapeutic applications.

www.msk.or.kr | 85

Symposia [S6] : Engineering Biology: The Dominant Approach to Engineer Biosystems for Bioeconomy

S6-6

A Strategic Blueprint for the Domestication of Geobacillus

stearothermophilus as a Thermophilic Platform Cell Using

the DNMB Suite

Jae-Yoon Sung1, Munhoe Lee1, Sang-Jae Lee2, Seong-Bo Kim3, and Dong-Woo Lee1*

1Department of Biotechnology, Yonsei University

2Department of Food Biotechnology and Research Center for Extremophiles & Marine Microbiology, Silla

University

3Bio-Living Engineering Major, Global Leaders College, Yonsei University

Geobacillus strains from the Bacillales order are facultative anaerobic thermophiles with

significant industrial applications, particularly in producing thermostable enzymes for sugar

utilization and degrading complex carbohydrates. Using the DNMB Suite and an expanded

genetic toolbox, we developed a plasmid artificial modification-based conjugation system and

effectively engineered the genetically challenging Geobacillus stearothermophilus with

GeoCas9EF. This engineered strain demonstrates optimized metabolic pathways, showing

great promise for biotechnological applications. The domestication strategies presented here

provide a framework for developing robust microbial platforms and encourage exploring diverse

species for industrial uses. Particularly, the utility of G. stearothermophilus in high-temperature

processes, such as producing D-tagatose and other rare sugars, highlights its potential in

food-grade applications and directed evolution. This research advances the field of thermophilic

microbiology, highlighting the vast potential of thermophiles for both theoretical and practical

applications. The future perspectives outlined suggest exciting opportunities for innovation

and further advancements in thermophilic applications.

Symposia [S7]

Co-organized by Korean Collection for Type Cultures

2024 International Meeting of the Microbiological Society of Korea

88 | www.msk.or.kr

S7-1

Culturing Novel Microbes Relevant to Environment:

Nitrifiers and Methanotrophs

Samuel Imisi Awala, Joo-Han Gwak, Christiana Abiola, Yongman Kim,

Ui-Ju Lee, and Sung-Keun Rhee*

Department of Microbiology, Chungbuk National University

Anthropogenic emissions of greenhouse gases (GHGs)—primarily carbon dioxide (CO2),

methane (CH4), and nitrous oxide (N2O)—are responsible for a historically rapid increase in

Earth’s average annual temperature by more than 0.2°C per decade. At the oxic–anoxic

interface of environments, reduced carbon and nitrogen compounds, such as methane and

ammonium, are produced. Methane emissions from wetlands are mostly mitigated by

methanotrophic bacteria. Aerobic methanotrophic bacteria were long assumed to have a

limited substrate spectrum, including methane, methanol, and occasionally other C1

compounds, but no other substrates. Ammonia-oxidizing microorganisms (AOM) play a crucial

role in the global nitrogen cycle in terrestrial environments and are responsible for the

production and emission of N

2O. The isolated AOM are obligate aerobes with

chemolithoautotrophic metabolism through NH3 oxidation and CO2 fixation. They have some

cultivated representatives and various subclusters that have not been cultured. In this talk, we

will share experiences of isolating fastidious novel microbes involved in aerobic methane and

ammonia oxidation.

www.msk.or.kr | 89

Symposia [S7] : From Shadow to Petri Dish: Breakthroughs in Culturing Elusive Microorganisms

S7-2

Microbial Dark Matter Mining: Challenges and Opportunities

Wen-Jun Li1,2

1State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou,

510275, P.R. China

2State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese

Academy of Sciences, Urumqi 830011, P.R. China

Microbes are the most abundant and diverse cellular life forms on Earth and colonize a wide

range of environmental niches. However, more than 99% of bacterial and archaeal species have

not been obtained in pure culture and we have only glimpsed the surface of this mysterious

microbial world. This is so-called Microbial Dark Matter (MDM): the enormous diversity of

yet-uncultivated microbes that microbiologists can only study by using cultivation-

independent techniques. This presentation will address why MDM deserves scientific efforts

and illustrates challenges and opportunities in the future study of these enigmas. I will also

introduce the research experiences on extremophiles which my team has gained, including

designing of new selective isolation media, in situ enrichment culture, simulation of

environmental culture system, and establishment of molecular ecology research technology

system.

2024 International Meeting of the Microbiological Society of Korea

90 | www.msk.or.kr

S7-3

How Culture Collections Diversify the Microbial Resources

Takashi Itoh*, Shigo Kato, and Moriya Ohkuma

Japan Collection of Microorganisms, RIKEN BioResource Research Center, Ibaraki, Japan

Japan Collection of Microorganisms (JCM) was originally founded in 1981, and joined in RIKEN

BioResource Center (currently, BioResource Research Center) in 2004. Since then, JCM has

collected preserved, quality-controlled and distributed diverse species of bacteria, archaea,

fungi useful for environmental and health sciences to contribute to scientific community. One

of its uniqueness would be that JCM has tried to hold phylogenetically and physiologically

diverse microorganisms. JCM has started collection of archaea and bacterial extremophiles from

early 1990’s, which include strictly anaerobic microorganisms such as methanogens. In addition,

JCM has accepted accessions of various fastidious microorganisms, such as a marine

microaerophic magnetotactic bacterium Magnetococcus marinus (JCM 17883T), an

ammonium-oxidizing archaeon Nitrososphaera gargensis (JCM 31473T), an obligately

piezophilic, anaerobic hyperthermophilic archaeon Pyrococcus yayanosii (JCM 16577T), and an

anerobic and syntrophic archaeon in the ‘Asgard’ lineage Promethearchaeum syntrophicum

(JCM 39240T), to name a few. The last two species names have been published validly by

depositing the type strains in JCM only as a publicly accessible culture collection. In the

meantime, we have also developed new microbial resources by ourselves. For example, we

isolated ectosymbiotic archaea, which grew on aerobic host archaeal cells, and deposited them

in culture collections to make available in the research communities (i.e., Nanobdella

aerobiophila JCM 33616T and Microcaldus variisymbioticus JCM 33787T).

Our measures to diversify and to promote utilization of the microbial resources will be

discussed.

www.msk.or.kr | 91

Symposia [S7] : From Shadow to Petri Dish: Breakthroughs in Culturing Elusive Microorganisms

S7-4

Exploration of Chemolithotrophs and Thermophiles from

Marine Environments; An Omnibus Story

Kae Kyoung Kwon1,2*, Yun Jae Kim1,2, Mi-Jeong Park1, Jae Kyu Lim1,2, Yeonng-Jun Park1,2,

Sung Hyun Yang1, Jhung-Ahn Yang1, and Jung-Hyun Lee1,2

1Marine Bioresources and Biotechnology Research Division, Korea Institute of Ocean Science &

Technology

2Major of Marine Convergence Technology, KIOST School, University of Science & Technology

Among the various application directions related to the industrial use of marine

microorganisms, autotrophic microorganisms linked to carbon neutrality and thermophilic

microorganisms capable of utilizing various enzymes were explored from hydrothermal vent

area as well as coastal areas. Thermococcus indicus IOT1

T isolated from the hydrothermal

environment of the central Indian Ocean ridge and related strains were confirmed to be involved

in the iron reduction, not well-known process in the genus Thermococcus. A number of

microorganisms, including Salidesulfovibrio onnuriiensis IOR2

T, a sulfur-reducing bacterium

isolated from the Indian Ocean hydrothermal environment, and Tissierellales bacterium

MEBiC14396T, isolated from rotting seaweed off the coast of Yeongdo Island, adopt a

mixotrophic system. In particular, in the case of strain MEBiC14396T, it seems closely related to

the growth and death of seaweed as it had low temperature tolerance and a large number of

glycosylation related genes (678 from 3344 CDSs). Additional microaerophilic and mesotrophic

sulfur oxidizing bacterium IOR34T affiliated with the order Chromatiales in Gammaoriteobacteria

was isolated from Onnuri vent Field in central Indian Ocean ridge and this strain use thiosulfate,

sulfur and sulfide as electron donors and use oxygen or nitrate as electron acceptors. In the

case of Onnuri Vent Field, strains belonging to Campylobacterales, a traditional sulfur-oxidizing

bacterial group, showed a relatively low proportion, and their role is possibly replaced by some

Gammaproteobacteria such as IOR34T, or members of the uncultivated phylum KSB1. By

cultivating new strains and studying their physiology, we can better understand what is

happening in the environment. Management of these strains is also very important, and KIOST

manages them through the Marine & Extreme Bioresource Collection and provides them to

related researchers.

[Supported by EA0212 of KIOST, PG54110 from MABIK & MOF 20170411]

Symposia [S8]

Co-organized by Professional Training Program

for Infectious Disease Research (MIST)

2024 International Meeting of the Microbiological Society of Korea

94 | www.msk.or.kr

S8-2

Korea Advanced Vaccine Development Center (KAVAD)

Roles in Pandemic Preparedness

Jae-Hwan Lim1,2

1Korea Advanced Vaccine Development Center

2Department of Vaccine Biotechnology, Andong National University

Here, we present the strategic initiatives undertaken by the Korea Advanced Vaccine

Development Center (KAVAD) established under KDCA to enhance pandemic preparedness

through the integration of advanced biotechnological and computational methods. Central to

our approach is the development of an artificial intelligence–powered antigen design platform

that employs machine learning algorithms to predict, design, and optimize potent vaccine

antigens with high immunogenicity and safety profiles.

This platform is seamlessly integrated with high-throughput screening systems and

supported by a comprehensive library of pre-evaluated vaccine candidates. Such integration

facilitates rapid antigen expression and validation across various vaccine modalities, including

mRNA, protein subunit, and viral vector platforms. Furthermore, KAVAD's repository of

designed and tested vaccine candidates serves as a crucial resource for immediate mobilization

against emerging threats. By maintaining a dynamic and scalable library of vaccine components,

we can quickly adapt to novel pathogens with minimal lead time, thereby enhancing our

capacity to tailor vaccine responses to specific antigens and variants. This integrated approach

not only reduces the time from antigen identification to clinical application but also strengthens

global health security by contributing to a more resilient and responsive vaccine development

ecosystem.

Our efforts position KAVAD at the forefront of global initiatives aiming to develop agile and

effective vaccine strategies. By combining artificial intelligence, high-throughput screening,

and advanced vaccine technologies, we demonstrate a viable pathway to rapidly counteract

future pandemics, ultimately contributing to improved public health outcomes worldwide.

www.msk.or.kr | 95

Symposia [S8] : Development of Vaccines against New and Variant Infectious Diseases

S8-3

Development of Innovative Vaccine and Safe Therapeutic

by mRNA Technology

Woo Ghil Lee

RNAGENE Inc.

RNAGENE is a Bio start-up company that has been conducting research and development on

mRNA-based vaccines and bio-drug since 2018. Currently, we are developing virus vaccines,

anti-cancer drug and rare disease drugs for humans and animals. At a technical point of view,

now, we have established the OUSPERA mRNA design platform for the efficient discovery and

development of various mRNA drugs and could maximize the expression of the gene of interest

in cell. This platform includes RPiC (RNA Panning in Cell) technology that allows screening of

mRNA constructs with optimized ORF sequences that can be stabilized within specific cells and

produce proteins efficiently. In this technology, the ORF sequence is highly randomized, leaving

the amino acid sequence unchanged. When mRNA vaccine and therapeutic are administered into

the body after LNP encapsulation, they are mostly delivered to the liver to produce proteins.

However, in specific applications such as gene editing, anti-cancer treatment and cell therapy,

the mRNA-LNP is required to specifically transport to cells or tissues of interest. For this

purpose, specific LNP components are used, or peptide and antibodies are chemically bound to

the LNP. However, we developed a differentiated delivery system that solves this problem in a

more general, easy, and non-chemical way. As a result, TARSPED technology were developed

for efficient delivery of mRNA-LNP to specific cell and tissues.

2024 International Meeting of the Microbiological Society of Korea

96 | www.msk.or.kr

S8-4

Success Factors for Rapid Development of mRNA Vaccine

against Diverse Infectious Disease within 100 Days

Kanghyun Choi, Joo Young Lee, Tae Gi Uhm, Minju Kwon, Sunhee Lee, Jisu Kim,

Dayeon Woo, Jieun Lee, Ye-Jin Yun, Hyemi Jo, Gyeongguk Park, Uk-Il Kim, Suyeon Song,

EunJi Kwon, Hojoon Lee, Suhyeon Kim, Yeonjin Yoo, Heejene Kim, Young Hwan Cho,

Hoon Woo Lee, Byungkyun Kim, Rachel Kim, Sungwon Kim, Jun Young Choi, Mooje Sung,

Kyungjin Kim, and Joo-Sung Yang*

ST PHARM

Messenger RNA (mRNA) vaccine represents a critical avenue for COVID-19 prevention. We

have developed a COVID-19 mRNA vaccine STP2104 encoding codon optimized full-length

S-type spike protein, which adopts our novel co-transcriptional 5’-cap reagent, SmartCap®

SC101. STP2104 has displayed potent neutralizing activity in immunized mice from PRNT50

assay. The protection efficacy of STP2104 in human ACE2 transgenic mice immunized and

challenged with SARS-CoV-2 represented good survival rate, virus titration and histopathology

study data. STP2104 results in human clinical trial phase I study demonstrated safety, and both

strong humoral and cellular immune responses. These safety and neutralizing antibody (NAb)

titer results support the efficiency and safety of SC101 in human, in addition to COVID-19

mRNA vaccine platform, we have patented novel SmartCap® library screening system and novel

lipid nanoparticle (LNP) STLNP®, capacity for mass production in GMP grade, and GMP

manufacturing facility for clinical trial vaccine production. Herein, we are presenting in-house

experience and know-hows of SmartCap® and STLNP

®, and their applications to COVID-19

mRNA vaccine programs, and Expedite 100-day project for rapid preparedness for

new/emerging infectious diseases.

www.msk.or.kr | 97

Symposia [S8] : Development of Vaccines against New and Variant Infectious Diseases

S8-5

Strategy to Develop Updated COVID-19 Vaccines Based on

Ad5/35 Platform for Effective Response to

Emerging Variants

Soojeong Chang, Kwang-Soo Shin, Bongju Park, Boyeong Song, Seowoo Park, Jieun Shin,

Hyemin Park, In Kyung Jung, Jong Heon Kim, Tae Wan Kim, and Chang-Yuil Kang*

Research & Development Center, Cellid Co., Ltd.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve,

with JN.1 and its subvariants including KP.2, KP.3, and LB.1, now emerging as predominant

variants globally. These newly emerging variants threaten the efficacy of available COVID-19

vaccines. To reduce the occurrence of breakthrough infections, re-infections, and disease

burden, it is essential to develop a strategy for producing updated vaccines that can provide

broad neutralization against both currently circulating and emerging variants. Using

phylogenetic trees based on spike protein sequences and antigenic cartography based on

neutralization, we selected variants that are antigenically distant from previously circulating

variants and developed updated variant-specific vaccines using an Ad5/35 platform-based

non-replicating recombinant adenoviral vector. We found that updated vaccines tailored by our

strategy exhibited improved neutralization ability compared to existing vaccines. Additionally,

we confirmed that vaccines targeting the JN.1, KP.2, KP.3, and LB.1 variants demonstrated

superior neutralizing activity against currently circulating variants compared to the

XBB.1.5-containing vaccine. These results highlight the importance of vaccine development to

keep pace with the evolution of SARS-CoV-2 variants and the need for updated vaccines.

Symposia [S9]

Sponsored by 농림축산식품부/농림식품기술기획평가원/

농생명마이크로바이옴혁신기술기반구축사업 & 과학기술정보통신부/

한국연구재단/다부처 국가생명연구자원 선진화사업

2024 International Meeting of the Microbiological Society of Korea

100 | www.msk.or.kr

S9-1

Changes in Microbiota Composition Due to Crop Rotation of

Tomatoes and Rice

Ismaila Yakubu1,2, Ji Ho Song1, Min A Son1, Yun Ju Lee1, Su Hyeon Han1, and Hyun Gi Kong1*

1Department of Plant Medicine, College of Agriculture, Life and Environment Science,

Chungbuk National University

2Department of Crop Protection, Faculty of Agriculture/ Institute for Agricultural Research,

Ahmadu Bello University, Zaria 810107, Nigeria

Microbial communities are known to have important functions in the health and growth of

plants. In particular, the rhizosphere microbial community is composed of various

microorganisms depending on the plant species and variety and has a close correlation with the

soil environment. In the case of tomatoes, the relationship between the microbial community

and soil-borne pathogenic bacterial wilt disease is known, but the presence or absence of

disease and comparison between varieties have been mainly studied. However, in the case of

soil diseases, research on soil environments as well as plants needs to be conducted in a

complex manner. In this study, we analyzed the correlation between the rhizosphere microbial

community and disease occurrence suppression according to the specific soil and crop

transformation of paddy-upland rotation, one of the tomato cultivation methods. The microbial

community was sequenced using the Miseq, and the microbiome analysis was performed using

Qiime2. As a result of the study, it was confirmed that Desulfobacterota increased and

Actinobacteriota decreased in the tomato rhizosphere rotation field compared to the

continuous cropping field. In addition, it was confirmed that the diversity and abundance of

microbial communities in the rhizosphere were higher than in the continuous cropping soil.

Furthermore, when soil chemical analysis was performed in the paddy-upland rotation soil and

the continuous cropping soil, it was confirmed that N source was higher in the continuous

cropping area, and S source and moisture were higher in the paddy-upland rotation soil. This

suggests the possibility that it is caused by the reorganization of microbial communities in the

anaerobic environment during rice cultivation. Therefore, the reorganization of microbial

communities in the soil infected with disease by paddy-upland rotation restores soil health and

shows the possibility of suppressing soil diseases.

www.msk.or.kr | 101

Symposia [S9] : Agricultural and Livestock Industrialization Based Microbiome

S9-2

Unraveling the Role of Seed Endophytes in

Shaping Tomato Health

Seongeun Yang, Heeyun Choi, and Kihyuck Choi*

Department of Applied Bioscience, Dong-A University

Seed endophytes play a crucial role in influencing seedling germination and growth in the

early stages of plant development, yet our understanding of the bacterial communities within

seeds, particularly those transmitted from parent to offspring, remains limited. Here, we

present a comprehensive study integrating in silico analysis and experimental validation to

explore the impact of seed endophytes on plant health, focusing on resistance to bacterial wilt

disease in tomato cultivars. We utilized in silico analysis to discern the OTUs shaping the

microbiota within seeds of both resistant (Hawaii 7996; H7996) and susceptible (Moneymaker;

MM) tomato cultivars. Leveraging this knowledge, we isolated seed endophytes from

surface-sterilized H7996 seeds and evaluated their dual capabilities in terms of antifungal

activity and plant growth promotion. Drawing from these insights, we engineered a synthetic

community (SynCom) exclusively composed of seed endophytes from H7996. This SynCom was

subsequently introduced into commercial seeds and monitored under gnotobiotic conditions

via taxonomic profiling utilizing 16S rRNA sequencing. Our results highlight the successful

establishment of SynCom within various plant compartments, showcasing its adaptability and

efficacy in different environmental contexts. Notably, the dominance of SynCom members was

observed to positively correlate with enhanced plant growth and resistance against Fusarium

oxysporum, the causative agent of bacterial wilt disease. This study underscores the potential

of harnessing seed endophytes, particularly through SynCom engineering, as a sustainable

approach towards bolstering plant health and combating prevalent agricultural diseases.

2024 International Meeting of the Microbiological Society of Korea

102 | www.msk.or.kr

S9-3

Animal Gut Microbiome Bank (AGMB)

Ju Huck Lee1*, Seung-Hwan Park1, Se Won Kang1, Yo Han Choi2,

Hyeun Bum Kim3, Jae-Kyung Kim4, and Ju-Hoon Lee5

1Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience

and Biotechnology

2National Institute of Animal Science

3Department of Animal Resources Science, Dankook University

4Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute

5Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology,

Center for Food and Bioconvergence, Seoul National University

As the gut microbiota have been shown to play a fundamental role in human health, the

population and diversity of gut microbiota in animals are also crucial for their health, including

nutritional, physiological, and immunological functions. Several studies have demonstrated that

the gut microbiota of livestock, such as pigs and cows, are strongly correlated with host fitness

and productivity across different growth stages. However, perturbations in gut microbiota,

known as dysbiosis, can lead to a loss of gut barrier function and increase the risk of pathogenic

infections.

While metagenomics, a culture-independent approach, has significantly contributed to

understanding the importance of gut microbiota in both human and animal health, there is a

growing recognition of the value of culture-dependent studies for isolating gut microbes.

Isolated gut microbes are essential for further research, such as verifying and characterizing the

functions of gut microbiota, and for the development of probiotics and pharmabiotics.

In light of this, we initiated the Animal Gut Microbiome Bank (AGMB) project in 2019 to

collect large-scale samples of animal gut microbiota from pigs and cows. To date, we have

isolated and preserved 11,111 strains comprising 586 species from pigs (4,788 strains), Korean

cows (4,152 strains), and dairy cows (2,171 strains). Starting last year, we have begun

distributing our isolated gut microbiota. Information on these microbes is available on the

AGMB homepage (https://www.kobic.re.kr/agmb/), and requests for distribution can be made by

contacting juhuck@kribb.re.kr

This talk will introduce and discuss the AGMB. Additionally, the functional characterization of

animal microbiota involved in pig growth will also be discussed.

www.msk.or.kr | 103

Symposia [S9] : Agricultural and Livestock Industrialization Based Microbiome

S9-4

The Effects of Selected Synbiotics on Gut Microbiota, and

Short-chain Fatty Acid Production of Weaned Pigs

Mirieom Park1,2, Soyeon Park1, Da Jung Lim1, Dae-Hyuk Kim1,3, and Yangseon Kim1*

1Department of Research and Development, Center for Industrialization of Agricultural and Livestock

Microorganisms

2Department of Animal Biotechnology, Jeonbuk National University

3Department of Molecular Biology and Bioactive Material Sciences, Jeonbuk National University

Prebiotic, probiotic, and symbiotic supplementation can be employted to influence both the

composition and function of gut microbiota, potentially leading to positive effects on the host’s

health. A total of 76 weaned pigs (Duroc × [Yorkshire × Landrace], average weight 7.36 ± 1.16

kg) were randomly allotted to one of four dietary treatments: a control group, a group fed with

the probiotic (Lactobacillus reuteri CACC607 1 × 108 CFU/g, and a prebiotic-fed group

(containing 3.3% Fructooligosaccharides, including levan and over 35% activated carbon) and a

synbiotic (pre-and probiotic) treated group. Microbiota analysis showed that dietary

supplementation with probiotics enhanced production and health-related genera compared to

the control group, while prebiotics showed a non-selective effect. Moreover, synbiotics a

combination of pre-and probiotics have significantly enriched the gut microbiota with

Psychrobacter, Prevotellaceae-NK3B31 group, Blautia, Alloprevotella, and Prevotella 7.

Importantly, increasing responses to fecal short-chain fatty acids were observed in the

synbiotic group (p < 0.05). In addition, as a result of hematological parameters, all treatment

groups showed values within the normal range.

Symposia [S10]

2024 International Meeting of the Microbiological Society of Korea

106 | www.msk.or.kr

S10-1

Effect of Enterotype-dependent Stability of the

Gut Microbiome on Post-gastrectomy Malnutrition in

Gastric Cancer Patients

Han Na Oh1,2,3, Woo Jun Sul3, Sang-Yong Son1, Sang-Uk Han1,

Hoon Hur1,4*, and Sun Gyo Lim5*

1Department of Surgery, Ajou University School of Medicine

2Inflamm-Aging Translational Research Center, Ajou University Medical Center

3Department of Systems Biotechnology, Chung-Ang University

4Department of Biomedical Science, Graduate School of Ajou University

5Department of Gastroenterology, Ajou University School of Medicine

Patients undergoing gastrectomy for gastric cancer (GC) are at risk of postoperative

malnutrition; however, the clinical presentation remains heterogeneous. Gastrectomy

contributes gut microbiome dysbiosis owing to the reduced gastric acid secretion, which

consequently leads to postoperative malnutrition. Here, we examined gut microbiome changes

in patients with GC who underwent gastrectomy and the impact of dysbiosis on

gastrectomy-induced malnutrition. From 82 patients who underwent curative gastrectomy for

GC, we collected fecal samples pre- and 1-year post-surgery while monitoring body weight and

nutritional parameters. Metagenome-assembled genomes and enterotype-specific methods

classified patients’ gut microbiota into Prevotella and Bacteroides enterotypes perioperatively.

Postoperatively, the patients in the Prevotella-rich group experienced more weight loss and

significant microbiota changes than those in the Bacteroides-rich group. Multivariate analysis

and AUC values supported that the pre-gastrectomy enterotype could predict the malnutrition,

and the gut microbial networks based on ASVs revealed the more fragile network in the

Prevotella-rich group. The preoperative gut microbiome status predicts postoperative

malnutrition in patients with GC who underwent gastrectomy. Thus, modifying the gut

microbiome perioperatively could mitigate post-gastrectomy malnutrition in patients with GC.

www.msk.or.kr | 107

Symposia [S10] : Microbiome

S10-2

Discovery and Annotation of Proteins from Microbiome

Baolei Jia1* and Che Ok Jeon2

1Xianghu Laboratory, Hangzhou 311231, P. R. China

2Department of Life Science, Chung-Ang University

Advances in technological and bioinformatics approaches have led to the generation of a

plethora of metagenomic datasets. Comparatively, the microbial enzymes mediating the

transformation of metabolites have not been intensively investigated. Here, we present the

recent efforts and technologies used for discovering and mining enzymes from the microbiome

data using three samples: bile salt hydrolases (BSHs) and lantibiotics. In BSHs study, 5,790 BSH

homologs were collected and classified into seven clusters based on a sequence similarity

network. The analysis also identified a significant association between BSH gene abundance and

10 human diseases, including gastrointestinal diseases, obesity, type 2 diabetes, liver diseases,

cardiovascular diseases, and neurological diseases. In lantibiotics study, we reported that

Streptococcus sp. C150 and Streptococcus vestibularis, which encode lantibiotics, are

potential risk factors for nonalcoholic fatty liver disease (NAFLD) and liver cirrhosis (LC), as

revealed by a comparison of the gut microbiome of healthy subjects (n= 2042) and patients

with diseases. Although many challenges lie ahead in this nascent and promising field,

deciphering the catalytic mechanisms and leveraging this knowledge to improve human health

are essential.

2024 International Meeting of the Microbiological Society of Korea

108 | www.msk.or.kr

S10-3

Multipartite Parasitic Interactions in the Aquatic Food Web:

Modulating Gene Exchange and Carbon Flow

Maliheh Mehrshad

Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Sweden

Parasitism is defined by an imbalanced profit margin where one partner benefits at the

expense of the other partner. Detailed studies highlight the importance of parasitic interactions

in gene and nutrient flow in the ecosystem and consequently challenge this binary status,

suggesting a continuum of interaction dynamics along mutualism and parasitism extremes.

Parasites are widespread in aquatic ecosystems with a notable diversity including viruses and

zoosporic eukaryotes. These parasites fine-tune their host’s dynamics and abundance, and

contribute to gene flow in the ecosystem. Their top-down trophic interactions with their host

facilitates carbon flow and modulate biogeochemical cycles however its functional basis

remains unknown. Majority of our existing knowledge is focused on two-partite parasitic

systems that is far from the complex natural interaction web. Studying interactions in a

multipartite perspective is challenged by lack of an operational framework. My research uses

multipartite parasitic model systems in culture and multi-omics analyses to understand the

functional basis of their interactions and its ecoevolutionary conseqeunces. I apply a framework

where in addition to tight co-evolutionary dynamics between pairs in a multipartite system,

system-level diffuse co-evolution is also considered. One of our multipartite model systems

contains two phages (genome sizes of 166 and 57 kb) co-infecting a single Limnohabitans

population. The larger phage represents lysogenic lifestyle and has the opposite codon usage

pattern compared to its host and carries all tRNAs needed to compensate for its different codon

usage pattern. The transcription profile of the whole pathosystem along the infection cycle

showed active transcription of all three constituents. Comparative transcriptomics suggests

that the small lytic phage uses addictive toxin-antitoxin systems to ensure its persistence in

the system.

[This work was supported by ERC starting grant (MULTIPHAGE), Swedish Research Council for

sustainable development (FORMAS), and Swedish Research Council (VR)]

www.msk.or.kr | 109

Symposia [S10] : Microbiome

S10-4

Gut Microbial Strain Resources and Potential Beneficials to

Host Health

Shuang-Jiang (SJ) Liu1,2* and his colleagues1,2

1State Key Laboratory of Microbial Technology, Qingdao, Shandong University, P. R. China

2Institute of Microbiology, Chinese Academy of Sciences, Beijing, P. R. China

There are millions of microbes living in human gut. Those microbes interact to each other and

further to human body, and they effect on host health and diseases. Gut microbial resources are

important for probiotics and living bacterial drugs, as well as for understanding the interactions

of gut microbiome and human body. This lecture will talk about the isolation, cultivation and

collection of gut microbial strains and evaluation their functions. Particularly,

Christensenellaceae has been detected in many human cohort studies, and was frequently

reported to be associated with host health. This presentation will discuss the cultivation and

collection of Christensenellaceae strains (ChrisGMB) and that how Christensenellaceae strains

are beneficial to host health. Two members of Christensenellaceae, Christensenella minuta, and

Luoshenia tenuis, will be presented in details.

[This work was supported by National Nature Science Foundation of China, Ministry of Science

and Technology, and Chinese Academy of Sciences.]

Symposia [S11]

Sponsored by the Next-generation Biorefinery Platform

Technologies Development Program (NRF, MSIT)

2024 International Meeting of the Microbiological Society of Korea

112 | www.msk.or.kr

S11-1

Simulations to Understand Enzyme Evolution and Drug

Resistance

Adrian Mulholland

Centre for Computational Chemistry, School of Chemistry, University of Bristol, UK

Molecular simulations can reveal biomolecular mechanisms and contribute to protein and

inhibitor design. Simulations can be computational ‘assays’ of biological activity, e.g. to predict

drug resistance or effects of mutation. Different methods are useful for different types of

problems. Combined quantum mechanics/molecular mechanics (QM/MM) identify mechanisms

of reaction and determinants of catalytic activity in enzymes, e.g. showing the role of the active

site electric field in determining carbapenemase activity of class A beta-lactamases.

Dynamical-nonequilibrium molecular dynamics (D-NEMD) simulations identify allosteric effects.

D-NEMD simulations of the SARS-CoV-2 spike protein show that the fatty acid binding site is

coupled to distant, functionally important regions. For the SARS-CoV-2 main protease,

D-NEMD simulations identify positions associated with drug resistance and an allosteric binding

site. In class A beta-lactamases, D-NEMD reveals allosteric networks and hotspots associated

with the spectrum of activity against antibiotics. D-NEMD simulations can identify sites for

mutation as a guide for enzyme engineering. Increasingly, simulations are contributing to

protein design, and to engineering and directed evolution of natural enzymes and de novo

biocatalysts. Simulations are also contributing to emerging evidence that activation heat

capacity is important in enzyme evolution and adaptation to specific temperatures.

[This work was supported by the European Research Council, BBSRC and EPSRC (UKRI)]

www.msk.or.kr | 113

Symposia [S11] : Engineering of Biocatalysts: Enzymes and Cells

S11-2

Protein Design of the Stevioside Converting Enzyme for

Activity and Stability

Seong-Ryung Go1,2, Gwang-Hyun Park1,2*, and Eui-Jeon Woo1,2*

1Department of Bioscience, KRIBB School, UST

2Disease Target Structure Research Center, KRIBB

Protein design is the rational construction of biomolecules that makes a ‘custom-built’

polypeptide for a particular function. The computational protein design seeks new structures

by searching sequences while exploring the folding space for the designed architecture with

minimum free energy. Enzymes play key catalytic roles in biological system and manufacturing

industries. UGT76 is a plant enzyme that converts the sweetner Stevioside to Rebaudiosides.

Herein, we utilized Rosetta, a computational molecular design program that calculates the

energy of a folded protein, to identify important regions related to the enzyme stability. We

introduced computational mutation scanning with position specific substitution matrix and

stabilizing factors such as core packing, helix capping, surface polarity and loop rigidity. The

resultant redesigned enzymes of transglycosylase UGT exhibits a significant increase of the

apparent Tm and the half-life as well as enhanced conversion of the product with a reduced

by-product. These results provide insights into computational redesign strategy of industrially

important enzymes such as the transglycosylase for improved stability and activity.

2024 International Meeting of the Microbiological Society of Korea

114 | www.msk.or.kr

S11-3

Engineering Perspectives on Methanotrophs for

Methane Bioconversion

Eun Yeol Lee

Department of Chemical Engineering, Kyung Hee University

Methane gas is considered a next-generation carbon substrate for the biosynthesis of

value-added products. Methanotrophs can be employed as the biocatalysts for methane

bioconversion. Methanotrophs are microorganisms that can use methane as a sole carbon and

energy source. In this presentation, engineering perspectives on methanotrophs will be

reviewed to evaluate the feasibility of methane bioconversion. Development of genetic tools

and genome-scale models for the efficient metabolic engineering of methanotrophs will be

presented. Some examples of applications including biodegradable plastics production from

methane gas will also discussed.

www.msk.or.kr | 115

Symposia [S11] : Engineering of Biocatalysts: Enzymes and Cells

S11-4

Discovery of New Biocatalyst for Hydrocarbon-based

Plastics Biodegradation

Soo-Jin Yeom

School of Biological Sciences and Technology, Chonnam National University

Plastic contamination currently threatens a wide variety of ecosystems and presents

damaging repercussions and negative consequences for many wildlife species. Sustainable

plastic waste management is an important approach to environmental protection and a

necessity in the current life cycle of plastics. In this regard, plastic biodegradation by

microorganism is most notable. This presentation includes current plastic pollution trends,

screening of plastic biodegradable new bacteria and the research hypothesis is that our

common area is already surrounded with a bunch of plastics, so it could be possible to screen

plastic-degrading bacteria, although a trash-contaminated uncommon environment might have

many plastic-degrading bacteria. In addition, we provide new plastic biodegradable enzymes

that revealed by NGS and transcriptome analysis to biodegrade polyethylene (PE). Furthermore,

we propose into plastic-eating synthetic bacteria for PE biodegradation. This strategy can be

applicable to other hydrocarbon-based plastics, such as polypropylene and polystyrene.

Symposia [S12]

2024 International Meeting of the Microbiological Society of Korea

118 | www.msk.or.kr

S12-1

Physiological Functions of RIG-I Like Receptors

Takashi Fujita

Laboratory of Regulatory Information, Institute for Life and Medical Sciences, Kyoto University, Japan

RIG-I like receptors (RLRs) are crucial for pathogen detection and triggering immune

responses and have immense physiological importance. I first summarize the interferon system

and innate immunity, which constitute primary and secondary responses. Next, the molecular

structure of RLR and the mechanism of sensing non-self RNA are described. Usually, self-RNA is

refractory to RLR; however, there are underlying host mechanisms that prevent immune

reactions. Studies have revealed that the regulatory mechanisms of RLR involve covalent

molecular modifications, association with regulatory factors, and subcellular localization.

Viruses have evolved to acquire antagonistic RLR functions to escape the host immune

reactions. Finally, the pathologies caused by the malfunction of RLR signaling will be described.

www.msk.or.kr | 119

Symposia [S12] : Exploring Basic Mechanisms and Applications in Viral Immunology, Vaccines, and Therapeutics

S12-2

A Rationally Designed Live Attenuated Vaccine Induces

Strong Mucosal Responses and Complete Protection

against SARS-CoV-2

Jinjong Myoung

Korea Zoonosis Research Institute, Jeonbuk National University

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), a zoonotic virus that

emerged from bats in 2019, has sparked a global pandemic, leading to billions of infections and

millions of deaths. Although the virus has transitioned to an endemic status, it continues to

present significant challenges. Existing mRNA vaccines face limitations, including reduced

efficacy against new variants like Omicron, inadequate mucosal IgA response, and reliance on

cold-chain storage. To address these issues, our study focused on developing a more effective

vaccine by targeting the virus’s entry point. We created a live attenuated vaccine using a

full-length infectious clone of SARS-CoV-2, incorporating 3-4 mutations to reduce its

virulence. After confirming attenuation in vitro and in vivo, we ensured the vaccine’s safety

through extensive passaging in Vero E6 cells. Immunogenicity was assessed in hACE2-

transgenic models with two intranasal doses, which induced strong levels of neutralizing

mucosal IgA and systemic IgG. Challenge tests with the wild-type virus showed no body weight

loss or mortality in vaccinated subjects, highlighting the vaccine’s potential efficacy and safety.

This innovative ‘Trojan Horse’ vaccine offers promise for protecting high-risk populations and

could help prevent the emergence of highly pathogenic SARS-CoV-2 variants.

2024 International Meeting of the Microbiological Society of Korea

120 | www.msk.or.kr

S12-3

A Novel Approach for Controlling Pulmonary Diseases by

Targeting the Newly Identified Lung-specific

Immunoreceptor

Ji-Seung Yoo

School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University

The innate immune system is the first line of the host defense program against pathogen

infection. Antiviral innate immune responses can be triggered by multiple cellular receptors that

recognize the viral components. The activated innate immune system produces interferons and

cytokines that perform antiviral functions to eliminate invading viruses by regulating the

activation of adaptive immunity. However, uncontrolled host immune responses may result in

pathological outcomes. Excessive production of interferons and cytokines due to aberrant

immune activation can inflict damage on tissues, eventually leading to the development of

multiple organ failure. Consequently, maintaining cellular homeostasis in immune responses is

critical in preserving overall health.

In this talk, I will present my recent research findings about a newly identified immunoreceptor

specific to the lungs. My study revealed that activation of the newly discovered immunoreceptor

can trigger severe pulmonary inflammation and tissue damage. Thus, targeting this immunoreceptor

can be a potential therapeutic strategy for controlling severe airway inflammatory diseases,

including COVID-19 and asthma.

www.msk.or.kr | 121

Symposia [S12] : Exploring Basic Mechanisms and Applications in Viral Immunology, Vaccines, and Therapeutics

S12-4

High-dimensional Analysis of Virus-specific T Cell

Responses by Mass Cytometry

Heeju Ryu

Department of Immunology, School of Medicine, Sungkyunkwan University

Understanding the complexity of T cell responses to viral infections and vaccinations is

critical for developing effective immunotherapies and vaccines. Mass cytometry (CyTOF), a

powerful single-cell analysis technology, enables high-dimensional characterization of antigen-

specific T cells, offering insights into their functional heterogeneity.

This presentation will highlight two studies utilizing mass cytometry to dissect virus-specific

T cell responses. In the first part of my presentation, I will discuss our work on tumor-specific T

cells in the blood of Merkel cell carcinoma patients, exploring their potential as predictive

biomarkers for checkpoint blockade immunotherapy. In the latter part, I will delve into how

pre-existing immunity from SARS-CoV-2 infection shapes T cell responses to mRNA

vaccination.

Together, these studies showcase the power of mass cytometry for in-depth analysis of

virus-specific T cell responses. This knowledge will contribute to the development of improved

vaccines and immunotherapies, ultimately leading to better control of viral infections and

cancers.

2024 International Meeting of the Microbiological Society of Korea

122 | www.msk.or.kr

S12-5

A Humanized ACE2 Mouse Model Recapitulating Age- and

Sex-dependent Immunopathogenesis of COVID-19

Uni Park1,2, Jae Hoon Lee3,4, Uijin Kim5, Kyeongseok Jeon1, Ga-Yeon Yoon5,

Hyun-Soo Cho5*, Han-Woong Lee3,4*, and Nam-Hyuk Cho1*

1Department of Microbiology and Immunology, Seoul National University College of Medicine

2Institute of Endemic Disease, Seoul National University Medical Research Center

3Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University

4GEMCRO Inc.

5Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University

In the ongoing battle against COVID-19, understanding its pathogenesis and developing

effective treatments remain critical challenges. The creation of animal models that closely

replicate human infection stands as a critical step forward in this research. Here, we present a

genetically engineered mouse model with specifically-humanized knock-in ACE2 (hiACE2)

receptors. This model, featuring nine specific amino acid substitutions for enhanced interaction

with the viral spike protein, enables efficient SARS-CoV-2 replication in respiratory organs

without detectable infection in the central nervous system. Moreover, it mirrors the age- and

sex-specific patterns of morbidity and mortality, as well as the immunopathological features

observed in human COVID-19 cases. Our findings further demonstrate that the depletion of

eosinophils significantly reduces morbidity and mortality, depending on the infecting viral dose

and sex of the host. This reduction is potentially achieved by decreasing the pathogenic

contribution of eosinophil-mediated inflammation, which is strongly correlated with neutrophil

activity in human patients. This underscores the model’s utility is studying the

immunopathological aspects of COVID-19 and represents a significant advancement in

COVID-19 modeling. It offers a valuable tool for testing vaccines and therapeutics, enhancing

our understanding of the disease mechanisms and potentially guiding more targeted and

effective treatments.

Symposia [S13]

2024 International Meeting of the Microbiological Society of Korea

124 | www.msk.or.kr

S13-1

Molecular Features and Intelligent Modeling for

Classification of Fungal Lifestyles

Jaeyoung Choi

Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University

Fungi exhibit a wide range of lifestyles, including roles as animal pathogens, plant pathogens,

and saprobes. These lifestyles may have shaped by adapting diverse habitats and co-evolving

through host-microbe interactions. Understanding the molecular basis of these lifestyles is

crucial, particularly for plant pathogens, which are critical to biosurveillance and risk

management. Recent studies have identified genomic signatures, including repetitive elements,

carbohydrate-active enzymes, biosynthetic gene clusters for secondary metabolites, and

oxidative enzymes, that are associated with specific fungal lifestyles. Leveraging these insights,

supervised machine learning models were developed to classify fungal lifestyles based on

genome annotation matrices. Dimensionality reduction techniques visualized distinctions for

taxonomic groups and fungal lifestyles therein. The identification of key features pivotal in

model performance might provide insights into the molecular determinants of specific

lifestyles. This analytical framework not only enhances our understanding of fungal biology but

also holds promises for broader applications in classifying other biological categories and

uncovering critical genomic features.

www.msk.or.kr | 125

Symposia [S13] : Fungal Genetics and Biology

S13-2

Discovery of Intrinsic and Extrinsic Drug Resistance

Principles through Clinical Fungal Isolates

Kyung-Tae Lee

Korea Zoonosis Research Institute, Jeonbuk National University

In the One-Health concept, wild animals serve as a crucial link between our environment and

the natural world, facilitating the transmission of numerous zoonotic diseases. Particularly

noteworthy is the reported phenomenon of widespread antibacterial agent application in

agricultural fields, which has been implicated in the development of microbial drug resistance

and subsequent dissemination via wildlife. The emergence of drug-resistant microorganisms in

urban areas, carried by wild animals, may lead to an increase in opportunistic infections. This

study aimed to isolate and identify diverse fungi from environmental, animal, and human

sources, examining their respective adaptive traits. Specifically, high-temperature-resistant

fungal strains were collected from hospital patients or environmental samples, and their drug

resistance profiles were compared and analyzed. Fungi were selectively cultured under acidic

and high-temperature conditions, inhibiting bacterial growth, and species identification was

achieved through ITS1 region sequencing. The isolated strains exhibited distinct phenotypic

characteristics from previously documented strains, with some demonstrating drug resistance.

The observed drug resistance was attributed to variations in gene expression, including genome

duplication, ergosterol biosynthesis, and glycerolipid metabolism. Additionally, increased

expression of proton antiporters was noted in certain cases. This study contributes to the

identification of novel control strategies by elucidating the presence and mechanisms of drug

resistance across various isolated fungal strains.

2024 International Meeting of the Microbiological Society of Korea

126 | www.msk.or.kr

S13-3

Factors Influencing the Nitrogen-source Dependent

Flucytosine Resistance in Cryptococcus Species

Dong-Hoon Yang1,2, Ami Khanal Lamichhane2, Kyung J. Kwon-Chung2, and Yun C. Chang2*

1Division of Biological Science and Technology, Yonsei University MIRAE Campus

2Molecular Microbiology Section, Laboratory of Clinical Immunology and Microbiology, National Institute

of Allergy and Infectious Diseases, Bethesda, Maryland, USA

Flucytosine (5-FC) is an antifungal agent commonly used for treatment of cryptococcosis

and several other systemic mycoses. In fungi, cytosine permease and cytosine deaminase are

known major players in flucytosine resistance by regulating uptake and deamination of 5-FC,

respectively. Cryptococcus species have three paralogs each of cytosine permease (FCY2,

FCY3, and FCY4) and cytosine deaminase (FCY1, FCY5 and FCY6). As in other fungi, we found

FCY1 and FCY2 to be the primary cytosine deaminase and permease gene, respectively, in C.

neoformans H99 (VNI), C. gattii R265 (VGIIa) and WM276 (VGI). However, when various amino

acids were used as the sole nitrogen source, C. neoformans and C. gattii diverged in the function

of FCY3 and FCY6. Though there was some lineage-dependent variability, the two genes

functioned as the secondary permease and deaminase, respectively, only in C. gattii when the

nitrogen source was arginine, asparagine, or proline. Additionally, the expression of FCY genes,

excluding FCY1, was under nitrogen catabolic repression in the presence of NH4. Functional

analysis of GAT1 and CIR1 gene deletion constructs demonstrated that these two genes

regulate the expression of each permease and deaminase genes individually. Furthermore, the

expression levels of FCY3 and FCY6 under different amino acids corroborated the 5-FC

susceptibility in fcy2Δ or fcy1Δ background. Thus, the mechanism of 5-FC resistance in C.

gattii under diverse nitrogen conditions is orchestrated by two transcription factors of GATA

family, cytosine permease and deaminase genes.

www.msk.or.kr | 127

Symposia [S13] : Fungal Genetics and Biology

S13-4

Identification of Essential Genes for the Establishment of

Spray-induced Gene Silencing (SIGS)-based Disease Control

in Fusarium graminearum

Sieun Kim1, Rowoon Lee2, Hosung Jeon2, Nahyun Lee2, Jiyeun Park2,

Heeji Moon2, Jiyoung Shin3, Kyunghun Min2, Jung-Eun Kim4,

Jung-Wook Yang5, and Hokyoung Son1,6*

1Horticultural and Herbal Crop Environment Division, National Institute of Horticultural & Herbal Science,

Rural Development Administration

2Department of Agricultural Biotechnology, Seoul National University

3Division of Bioresources Bank, Honam National Institute of Biological Resources

4Research Institute of Climate Change and Agriculture, National Institute of Horticultural and Herbal

Science

5Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural

Development Administration

6Research Institute of Agriculture and Life Sciences, Seoul National University

The plant pathogenic fungus Fusarium graminearum causes Fusarium head blight (FHB) in

major cereal crops such as wheat, barley, and rice, leading to considerable economic losses. As

resistance to chemical fungicides in F. graminearum continues to rise, there is an increasing

demand for the development of novel disease control strategies. To discover essential genes

that could serve as disease control targets, we focused on genes that had proven difficult to

delete in previous studies. We confirmed the essentiality of thirteen genes using either

conditional promoter replacement (CPR) mutants or a CRISPR/Cas9-mediated editing approach.

We synthesized double-stranded RNAs (dsRNAs) targeting these essential genes and analyzed

their protective effects in plants using a spray-induced gene silencing (SIGS) method. Notably,

when dsRNAs targeting Fg10360, Fg13150, and Fg06123 were applied to detached barley

leaves prior to fungal inoculation, there was a marked reduction in disease lesions. Our findings

provide evidence of the potential of essential genes identified by a SIGS method to be effective

targets for controlling fungal diseases.

Symposia [S14]

Sponsored by Research Collaboration Center for K-Beauty

2024 International Meeting of the Microbiological Society of Korea

130 | www.msk.or.kr

S14-1

Extracellular Vesicles of Limosilactobacillus fermentum

SLAM216 Ameliorate Skin Symptoms of Atopic Dermatitis

by Regulating Gut Microbiome on Serotonin Metabolism

Younghoon Kim

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul

National University

In this study, we investigated the effects of probiotic-derived extracellular vesicles on atopic

dermatitis (AD). Initially, we isolated and characterized extracellular vesicles from Limosilactobacillus

fermentum SLAM 216 (LF216EV) and characterized their composition through multi-omics

analysis. Importantly, in an atopic dermatitis-like cell model induced by TNFα/IFNγ, LF216EV

significantly modulated the expression of immune regulatory genes, indicating its potential

functionality in atopic dermatitis. LF216EV alleviated AD-like phenotypes, such as redness,

scaling/dryness, and excoriation, induced by DNCB. Histopathological analysis revealed that

LF216EV decreased epidermal thickness and mast cell infiltration in the dermis. Furthermore,

LF216EV administration reduced mouse scratching and depression-related behaviors, with a

faster onset than the classical treatment with dexamethasone. In the quantitative real-time

polymerase chain reaction (qRT-PCR) analysis, we observed a significant increase in the

expression levels of htrb2c, sert, and tph-1, genes associated with serotonin, in the skin and gut

of the LF216EV-treated group, along with a significant increase in the total serum serotonin

levels. Gut microbiome analysis of the LF216EV-treated group revealed an altered gut

microbiota profile. Correlation analysis revealed that the genera Limosilactobacillus and

Desulfovibrio were associated with differences in the intestinal metabolites, including

serotonin. Our findings demonstrate that LF216EV mitigates AD-like symptoms by promoting

serotonin synthesis through the modulation of gut microbiota and metabolome composition.

www.msk.or.kr | 131

Symposia [S14] : Unravelling the Relationship between the Gut Microbiome and Skin Health: Gut-Skin-Immune Axis

S14-2

Probiotics as Supplemental Preventive and Therapeutic

Agents against Primary Dysmenorrhea

Jinok Kwak1, Hyunok Doo1, Gi Beom Keum1, Yejin Choi1, Juyoun Kang1, Haram Kim1,

Yeongjae Chae1, Sheena Kim1, Ju-Hoon Lee2, and Hyeun Bum Kim1*

1Department of Animal Biotechnology, Dankook University

2Department of Food and Animal Biotechnology, Seoul National University

Primary dysmenorrhea (PD) is defined as periodic cramps and pain originating from the uterus

during the menstrual period, and it is the most common gynecological condition among women

of reproductive age. This study aimed to evaluate probiotics as preventive and therapeutic

agents against PD using a dysmenorrhea mouse model. We isolated and selected three

candidate beneficial microbes (Lactiplantibacillus plantarum, Lactobacillus gasseri, and

Bifidobacterium animalis subsp. lactis) through in vitro experiments, then evaluated these three

bacteria using an in vivo model. The results of this study showed that writhing scores (an

indicator of pain severity) and PGF2α (one of the most representative pathological factors

causing dysmenorrhea) levels in serum decreased with probiotic treatment. In addition,

histological examination of the uterus revealed that endometrial thickness and the number of

glands decreased with probiotics treatment compared to the positive control. Overall, our data

indicate that the three probiotics used in this study have the potential to be used as preventive

and therapeutic agents against PD.

2024 International Meeting of the Microbiological Society of Korea

132 | www.msk.or.kr

S14-3

Characterization of Exopolysaccharide Produced by

Probiotics and Its Protective Effects on Skin Damage

Jin Hwan Kim and Nam Su Oh*

Department of Food and Biotechnology, Korea University, Sejong

Exopolysaccharides (EPSs) are extracellular macromolecules produced by the growth and

metabolism of bacteria, yeast, fungi, and other microorganisms. Recently, EPSs derived from

probiotic bacteria have received increasing attention because of their combined probiotic

activities, including antioxidant, antitumor, and anti-inflammatory properties that improve

human health. The skin serves as the primary barrier in human body and is constantly exposed

to various risk factor. Ultraviolet-B radiation (UVB) induces the generation of reactive oxygen

species (ROS), which can directly or indirectly damage DNA, protein, and other molecules,

leading to cell apoptosis, mutation, and inflammation. In this study, we investigated the effects

of EPS isolated from fermented product with Lacticaseibacillus rhamnosus IR06(EPS-IR06) in

UVB-irradiated human skin cells. Initially, we evaluated the potential protective effects of six

EPS isolated from Lactobacillus species against skin damage in in vitro assays. EPS-IR06 showed

high inhibitory activity of enzyme related skin aging, particularly collagenase. Furthermore,

EPS-IR06 effectively suppressed ROS production and decreased the mRNA expression of matrix

metalloproteinases (MMPs) and pro-inflammatory cytokines in UVB-irradiated HaCaT cells.

EPS-IR06 alleviated UVB-induced photoaging and inflammation by regulating the

mitogen-activated protein kinase (MAPK) and nuclear factor kappa beta (NF-κB) signaling

pathway. We identified EPS-IR06 is glucose-branched with a galactomannan backbone

consisting of nine monosaccharides repeating units, including α-D-Manp, α-D-Galp, and β

-D-Glcp. The average molecular weight of EPS-IR06 was estimated to be 4.8 × 105 Da and

EPS-IR06 was composed of 41.1% of mannopyranose, 34.3% of galactopyranose, and 24.6% of

glucopyranose.

www.msk.or.kr | 133

Symposia [S14] : Unravelling the Relationship between the Gut Microbiome and Skin Health: Gut-Skin-Immune Axis

S14-4

Integrating Metabolomics Analysis in Synbiotics Research:

Unveiling Postbiotics’ Role in Skin Wrinkle Reduction

Hakdong Shin

Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong

University

Postbiotics, the bioactive compounds generated by beneficial microorganisms during

fermentation, have emerged as key players in health research due to their potential benefits,

driving growing interest in understanding their metabolic interactions within synbiotic

combinations. Furthermore, the variations in individual gut microbiome structures suggest the

necessity of developing precision probiotics. In this study, we constructed an in vitro model

using Korean gut microbiome incubation to screen for synbiotic combinations and related

metabolite profiling. The microbiota and metabolomic data obtained in this study provide

insights into the effects of potential prebiotic materials on changes in gut microbiome and

metabolites. Additionally, metabolomics technology was applied to analyze metabolites

produced by candidate probiotic strains, and compounds significantly increased by prebiotic

treatment were identified. The study further investigates the signaling pathways involved in

collagen production or degradation regulation within skin cells, screening for postbiotics that

show wrinkle improvement effects. Overall, this study aims to offer effective strategies for

utilizing metabolomics analysis in exploring synbiotic combinations based on individual gut

microbiome information, focusing on their role in skin health, particularly in enhancing collagen

production and wrinkle reduction.

Symposia [S15]

2024 International Meeting of the Microbiological Society of Korea

136 | www.msk.or.kr

S15-1

Unexpected Implications and Applications of

Methanotrophy

Jeremy D. Semrau

Department of Civil & Environmental Engineering, The University of Michigan, USA

Methanotrophs are an intriguing group of microbes with the remarkable ability to consume

methane as their sole source of carbon and energy. As such, methanotrophs are receiving

increased attention to control methane emissions to limit future climate change.

Methanotrophs, however, also have a wide range of other applications, including pollutant

remediation and methane valorization (e.g., conversion of methane to protein as well as

precursors of plastics and diesel amongst other products), and a great deal of effort is being put

forward to capitalize on various aspects of methanotrophy. Any attempt to do so, however,

must consider that methanotrophic activity is affected by a wide range of metals, especially

copper. Indeed, methanotrophs have been shown to produce unique secondary metabolites for

the collection of copper, and this may be perhaps the most significant application of

methanotrophy. That is, some methanotrophs produce a compound called methanobactin for

the collection of copper and we have shown that methanobactin has substantial promise for

the treatment of copper-related human ailments. In this presentation I will describe: (1) how

methanotrophic activity and physiology is affected by copper, (2) the diversity of

methanobactins produced by methanotrophs, and (3) the development of innovative strategies

to not only increase methanobactin production, but also to create novel forms of

methanobactin that can enhance its use as a therapeutic compound.

[This work was supported by grants from the United States National Science Foundation

(#1912482 and #2418164) and the United States Department of Energy (#DE-SC0020174)]

www.msk.or.kr | 137

Symposia [S15] : Environmental Microbiology

S15-2

Raman-based Identification and Isolation of

Microorganisms of Interest in Complex Microbial

Communities

Kang Soo Lee

Department of Mechanical Engineering, UNIST

Raman microspectroscopy offers an efficient, non-invasive, and often label-free approach to

investigate the molecular composition of samples. Recent advancements in technology and

data analysis in Raman microspectroscopy have significantly improved the sensitivity and

flexibility of measuring microorganisms as small as a few hundred nanometers and identifying

dilute macromolecules of interest (e.g., carbohydrates, proteins, lipids, nucleic acids, and

pigments) within aqueous cell environments. In this talk, I will discuss how Raman

microspectroscopy has been utilized to explore key physiological aspects (e.g., metabolic

activity and cellular processes) of microorganisms within complex microbial communities. This

identification process is integrated into an automated system for the targeted sorting of cells

of interest, known as Raman-activated cell sorting (RACS). This system combines optical

tweezers, microfluidics, stable isotope probing, and software programming, followed by

downstream -omics analysis and cultivation for further ecological evaluations. As an example, I

will showcase the application of this platform in sorting mucin-degrading bacteria from

mammalian intestines and nitrite-oxidizing bacteria from ocean environments. This platform

establishes a direct link between the phenotypes and genotypes of cells of interest within

complex microbial communities, opening new avenues for microbial ecology research.

2024 International Meeting of the Microbiological Society of Korea

138 | www.msk.or.kr

S15-3

Bacterial Quantal Secretion through Membrane Vesicles

Masanori Toyofuku

Faculty of Life and Environmental Sciences, Microbiology Research Center for Sustainability,

University of Tsukuba, Tsukuba, Japan

Most bacteria release membrane vesicles (MVs) that play crucial roles in inter-bacterial and

bacterial-host interactions by delivering cargo molecules.

Several studies have shown that these MVs are essential for the release and transport of

hydrophobic substances and enable packaged deliver of different molecules. Given the feature

of MVs in packaging the cargo and maintaining their concentration, such MV mediated secretion

system can be considered as quantal secretion. Quantal secretion is distinct from canonical

secretion systems in bacteria, where the molecules are diluted upon secretion from the cells.

So far, we have been studying the formation, function and delivery of MVs and have found

that MVs can be formed through cell death. This process requires degradation of the

peptidoglycan in both Gram-negative and Gram-positive bacteria, which process were named

explosive cell lysis and bubbling cell death, respectively. In addition to cell death mediated MV

formation, the blebbing of the outer membrane is well studied as a MV formation route in

Gram-negative bacteria. We have also found that MVs are involved in delivering hydrophobic

signaling used in bacterial communications and involved in iron acquisition. In this talk, I would

like to mainly introduce our studies on formation and functions of MVs.

www.msk.or.kr | 139

Symposia [S15] : Environmental Microbiology

S15-4

Polymyxin B-Induced Alteration in Bacterial Cell

Membranes

Yongjun Son1†, Bitnara Kim1†, Pureun Kim1†, Jihyeon Min1, Yerim Park1,

Jihye Yang1, Masanori Toyofuku2, and Woojun Park1*

1Department of Environmental Science and Ecological Engineering, Korea University

2Department of Life and Environmental Sciences, University of Tsukuba, Japan

Gram-positive Enterococcus faecium exhibited higher susceptibility to polymyxin B (PMB),

the canonical antimicrobial peptide against Gram-negative bacteria, under anaerobic conditions

than aerobic conditions. Anaerobically grown E. faecium exhibited high vulnerability to PMB,

leading to significant cell surface damage, as observed based on their high dansyl-PMB affinity,

fluorescence-activated cell sorting results, and scanning electron microscopy findings.

Interestingly, our transcriptomic and chemical analyses revealed that enterocin B, produced

anaerobically, imposes a burden on the cellular envelope when cells are exposed to PMB. This

scenario was also supported by PMB susceptibility tests and killing curves, which showed that

ΔentB knockout mutant cells were more resistant to PMB (32 µg/ml) compared to wild-type

cells (4 µg/ml) under anaerobic condition. Fluorescent D-amino acid and BOCILLIN™

-fluorescent profiling of transpeptidase activities in ΔentB mutant cells under anaerobic

condition revealed similar levels of activity to those observed in WT cells under aerobic

condition. The high levels of secreted bacteriocins in WT under anaerobic condition likely lead to

significant loosening of the peptidoglycan layer, making it more susceptible to PMB. Overall, our

findings suggest that anaerobically produced bacteriocins, in conjunction with PMB, contribute

to the killing of E. faecium by destabilizing its cell envelope.

Symposia [S16]

2024 International Meeting of the Microbiological Society of Korea

142 | www.msk.or.kr

S16-1

Do Plasmids Pick a Bacterial Partner before Committing to

Conjugation?

Wen Wen Low1, Sophia David2, Chloe Seddon1, Joshua Wong1,

Konstantinos Beis1, and Gad Franke1*

1Department of Life Sciences, Imperial College London, London, UK

2Big Data Institute, University of Oxford, Oxford, UK

Horizontal gene transfer plays a key role in bacterial evolution and the spread of antibiotic

resistance genes (ARGs), among which conjugation is the major driver. During conjugation

plasmid DNA is transferred unidirectionally from one bacterium (donor) to another (recipient) in

a contact-dependent manner. The conjugation process is divided into 3 phases: phase 1 occurs

exclusively within the donor, involving the assembly of a type IV secretion system (T4SS) and

the conjugation pilus. Phase 2 encompasses both the donor and recipient, where initial contact

is mediated by the pilus (known as mating pair formation - MPF) leading to inefficient DNA

transfer. This is followed by TraN-mediated mating pair stabilisation (MPS) and efficient plasmid

transfer. Phase 3 occurs within the recipient, where following expression of plasmid genes it

becomes refectory to a second wave of conjugation by the same plasmid. We have recently

described the mechanism by which TraN not only mediates MPS but also plasmid host range. We

have also solved the cyroEM structure of TraT which mediates surface exclusion in phase 3. In

this seminar I will focus of structure/function data of TraN and TraT during conjugation of IncF

(as well as IncH and IncA/C) plasmids.

[This work was supported by the Wellcome Trust]

www.msk.or.kr | 143

Symposia [S16] :

Structure and Function of Microbial Proteins

S16-2

Managing the Number of Specialized Ribosomes

Yoon-Mo Yang

Graduate School for Biomedical Science & Engineering, Hanyang University

Rps26-deficient ribosomes are a physiologically relevant ribosome population which arises

during osmotic stress to support the translation of mRNAs involved in the response to high salt

in yeast. They are formed by binding of the chaperone Tsr2 to fully assembled ribosomes to

release Rps26 when intracellular Na+ concentrations rise. Tsr2-mediated Rps26 release is

reversible, enabling a rapid response that conserves ribosomes. However, because the

concentration of Tsr2 relative to ribosomes is low, how the released Rps26•Tsr2 complex is

managed to allow for accumulation of Rps26-deficient ribosomes to nearly 50% of all

ribosomes remains unclear. Here we show that released Rps26 is degraded via the

Pro/N-degron pathway, enabling the accumulation of Rps26-deficient ribosomes. Substitution

of the N-terminal proline of Rps26 to serine increases the stability of free Rps26, limits the

accumulation of Rps26-deficient ribosomes and renders yeast sensitive to high salt. The

GID-complex, an E3 ubiquitin ligase, and its adaptor Gid4, mediate polyubiquitination of Rps26

at Lys66 and Lys70. Moreover, this ubiquitination event is required for Rps26 degradation, the

accumulation of Rps26-deficient ribosomes and the high salt stress resistance. Together, the

data show that targeted degradation of released Rps26 from the Rps26•Tsr2 complex allows

Tsr2 to be recycled, thus facilitating multiple rounds of Rps26 release.

2024 International Meeting of the Microbiological Society of Korea

144 | www.msk.or.kr

S16-3

Activation of Zinc Uptake Regulator by Zinc Binding to

Three Regulatory Sites

Yunchan Choi1, Junseock Koh2*, Sun-Shin Cha3, and Jung-Hye Roe1*

1Laboratory of Molecular Microbiology, School of Biological Sciences, Seoul National University

2Laboratory of Molecular Microbiology, School of Biological Sciences, Seoul National University

3Protein Research Laboratory, Department of Chemistry and Nanoscience, Ewha Womans University

Zur is a Fur-family metalloregulator that is widely used to control zinc homeostasis in

bacteria. In Streptomyces coelicolor, Zur (ScZur) acts as both a repressor for zinc uptake (znuA)

gene and an activator for zinc exporter (zitB) gene. Previous structural studies revealed three

zinc ions specifically bound per ScZur monomer; a structural one to allow dimeric architecture

and two regulatory ones for DNA-binding activity. In this study, we present evidence that Zur

contains a fourth specific zinc-binding site with a key histidine residue (H36), widely conserved

among actinobacteria, for regulatory function. Biochemical, genetic, and calorimetric data

revealed that H36 is critical for hexameric binding of Zur to the zitB zurbox and further binding

to its upstream region required for full activation. A comprehensive thermodynamic model

demonstrated that the DNA-binding affinity of Zur to both znuA and zitB zurboxes is

remarkably enhanced upon saturation of all three regulatory zinc sites. The model also predicts

that the strong coupling between zinc binding and DNA binding equilibria of Zur drives a

biphasic activation of the zitB gene in response to a wide concentration change of zinc. Similar

mechanisms may be pertinent to other metalloproteins, expanding their response spectrum

through binding multiple regulatory metals.

www.msk.or.kr | 145

Symposia [S16] :

Structure and Function of Microbial Proteins

S16-4

An Archaeal Transcription Factor EnfR with a

Novel ‘Eighth Note’ Fold Controls Hydrogen Production

of a Hyperthermophilic Archaeon

Thermococcus onnurineus NA1

Da-Woon Bae1†, Seong Hyuk Lee2†, Ji Hye Park3, Se-Young Son1, Yuxi Lin5,

Jung Hyen Lee3, Bo-Ram Jang4, Kyu-Ho Lee4, Young-Ho Lee5, Hyun Sook Lee2,

Sung Gyun Kang2, Byoung Sik Kim3, and Sun-Shin Cha1*

1Department of Chemistry & Nanoscience, Ewha Womans University

2Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology

3Department of Food Science and Biotechnology, Ewha Womans University

4Department of Life Science, Sogang University

5Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI)

Thermococcus onnurineus NA1, a hyperthermophilic carboxydotrophic archaeon, produces

H2 through CO oxidation catalyzed by proteins encoded in a carbon monoxide dehydrogenase

(CODH) gene cluster. TON_1525 with a DNA-binding helix-turn-helix (HTH) motif is a putative

repressor regulating the transcriptional expression of the codh gene cluster. The T55I mutation

in TON_1525 led to enhanced H2 production accompanied by the increased expression of genes

in the codh cluster. In this study, TON_1525 was demonstrated to be a dimer. Monomeric

TON_1525 adopts a novel ‘eighth note’ symbol-like fold (referred to as ‘eighth note’ fold

regulator, EnfR), and the dimerization mode of EnfR is unique in that it has no resemblance to

structures in the Protein Data Bank. According to footprinting and gel shift assays, dimeric EnfR

binds to a 36-bp pseudo-palindromic inverted repeat in the promoter region of the codh gene

cluster, which is supported by an in silico EnfR/DNA complex model and mutational studies

revealing the implication of N-terminal loops as well as HTH motifs in DNA recognition. The

DNA-binding affinity of the T55I mutant was lowered by ~15-fold, for which the conformational

change of N-terminal loops is responsible.

2024 International Meeting of the Microbiological Society of Korea

146 | www.msk.or.kr

S16-5

PhoPQ-Mediated Modification of the Lipopolysaccharide

Inner Core Affects Intrinsic Resistance to Tetracycline

Antibiotics and Bile Salts in Escherichia coli

Umji Choi and Chang-Ro Lee*

Department of Biological Sciences, Myongji University

Lipopolysaccharide (LPS) modification is important for adaptation to extracellular stresses

and antibiotic resistance, such as colistin resistance. Despite the physiological and clinical

importance of LPS modification, physiological roles of modifications of the LPS core region

remain unclear. In this study, we elucidated the role of the modification of the LPS inner core in

intrinsic resistance to tetracycline and glycylcycline antibiotics and bile salts with a fused

tetracyclic nucleus in Escherichia coli. Inactivation of the PhoPQ two-component system, which

governs the transcription of various genes involved in magnesium transport and LPS

modification, induces increased susceptibility to tetracycline, doxycycline, tigecycline,

minocycline, and bile salts. These phenotypes are caused by enhanced expression of

phosphoethanolamine transferase EptB, which catalyzes the modification of the inner core

sugar of LPS. Disruption of EptB increases resistance to tetracycline and glycylcycline

antibiotics, whereas the other two phosphoethanolamine transferases, EptA and EptC, that

participate in the modification of other LPS residues, are not associated with resistance to

tetracyclines and glycylcycline. Overall, our results demonstrated that PhoPQ-mediated

modification of a specific residue of LPS by phosphoethanolamine transferase EptB regulates

intrinsic resistance to tetracycline and glycylcycline antibiotics and bile salts with a fused

tetracyclic nucleus.

[This work was supported by a research grant from Basic Science Research Program through

the National Research Foundation of Korea funded by the Ministry of Education (NRF-RS-

2023-00246684)]

Symposia [S17]

Sponsored by Sanigen Co., Ltd

2024 International Meeting of the Microbiological Society of Korea

148 | www.msk.or.kr

S17-1

Inflammation-mediated Microbiota Killing Drives

Aspartate-dependent Salmonella Typhimurium Expansion

in the Inflamed Gut

Woongjae Yoo

Department of Life Sciences, Pohang University of Science and Technology (POSTECH)

Inflammation boosts availability of electron acceptors in the intestinal lumen creating a

favorable niche for pathogenic Enterobacteriaceae. However, the mechanisms linking intestinal

inflammation-mediated changes in luminal metabolites and pathogen expansion remain unclear.

Here, we show that mucosal inflammation induced by Salmonella enterica serovar Typhimurium

(S. Tm) infection increases intestinal levels of the amino acid aspartate. S. Tm used aspartate

ammonia-lyase (aspA)-dependent fumarate respiration for growth in the murine gut only during

inflammation. AspA-dependent growth advantage was abolished in the gut of germ-free mice

and restored in gnotobiotic mice colonized with members of the classes Bacteroidia and

Clostridia. Reactive oxygen species produced by the host response caused lysis of commensal

microbes, resulting in the release of microbiota-derived aspartate that was used by S. Tm, in

concert with nitrate-dependent anaerobic respiration, to outcompete commensal

Enterobacteriaceae. Our findings demonstrate the role of microbiota-derived amino acids in

driving respiration-dependent S. Tm expansion during colitis.

www.msk.or.kr | 149

Symposia [S17] : Innovative Approaches to Microbiome-Human Interactions Using Next Generation Sequencing

S17-2

RBC Interplay in Pathogen-induced Thrombosis

Han Young Chung

College of Pharmacy, Chungnam National University

Patients infected with Vibrio vulnificus frequently experience hemolytic anemia and vascular

complications, including venous thrombosis. Despite this, the underlying mechanisms linking V.

vulnificus infection to venous thrombosis remain poorly understood. In this study, we observed

that subhemolytic levels of V. vulnificus infection prompted significant morphological

alterations in human red blood cells (RBCs), leading to phosphatidylserine exposure and

microvesicle formation. These changes facilitated the procoagulant activation of RBCs, thereby

enhancing their thrombogenic potential. Importantly, when RBCs were exposed to V. vulnificus,

there was a notable upregulation of the rtxA gene, which encodes the multifunctional

autoprocessing repeats-in-toxin (MARTX). Mutational analyses revealed that the procoagulant

activity induced in RBCs by V. vulnificus is attributable to the pore-forming domain of the

MARTX toxin, which induces intracellular calcium influx in RBCs. Using a rat model of venous

thrombosis induced by tissue factor and stasis, we demonstrated that wild-type V. vulnificus

significantly promoted thrombosis, whereas a ΔrtxA mutant strain did not, confirming that V.

vulnificus promotes thrombosis through the MARTX toxin-mediated activation of RBCs.

2024 International Meeting of the Microbiological Society of Korea

150 | www.msk.or.kr

S17-3

Molecular Mechanisms of the Intestinal Epithelium in

Regulating Nutrient-Microbiota-Immune Interactions

Ye-Ji Bang1,2

1Department of Biomedical Sciences, Seoul National University College of Medicine

2Department of Microbiology and Immunology, Seoul National University College of Medicine

The intricate interplay between the intestinal epithelium and microbiota critically regulates

immune responses, profoundly impacting health and disease. This study explores the pivotal

functions of intestinal epithelial cells in sensing and responding to microbial and nutritional

signals, elucidating how these interactions shape intestinal immunity. We investigate the

mechanisms by which dietary nutrients, with a focus on vitamin A, modulate epithelial-immune

crosstalk. Our findings reveal that vitamin A enhances mucosal immunity through the regulation

of serum amyloid A and retinol transportation. Additionally, we uncover a novel pathway

through which the intestinal epithelium orchestrates nutrient utilization and antimicrobial

defense. These discoveries illuminate the complex network of epithelial-microbial-immune

interactions, underscoring the crucial role of nutritional and microbial environments in

maintaining immune homeostasis. Our research not only advances the understanding of

intestinal barrier function but also opens new avenues for targeted interventions in the

prevention and treatment of immune-mediated diseases.

www.msk.or.kr | 151

Symposia [S17] : Innovative Approaches to Microbiome-Human Interactions Using Next Generation Sequencing

S17-4

DATA Driven Foodborne Pathogen Detection Using NGS

Technology and Its Modification

Eun Kyoung Shin and Jinho Choi*

R&D Center, Sanigen Co., Ltd.

Including Salmonella there are at least 25 harmful microorganisms in food and its

manufacturing condition and the risk should be well controlled for the safe food consumption.

According to World Health Organization, global burden of foodborne illness is estimated as 600

million cases and 420 thousand deaths. Among the victims, 30% of the death occur under the

age 5. Therefore fast, efficient, and accurate detection method is needed for the monitoring the

foodborne and zoonotic pathogens. Until now, multiplex PCR can detect 4 to 5 pathogens at

one tube reaction, because of the limitation of the fluorescence dye interference. Also, it has

NTC (no template control) contamination problem when performing the detection test.

Moreover, if there are many samples at one time, the complexity of the experiment is doubled.

However, next generation sequencing (NGS) panel can detect multiple samples and dozens of

microorganisms with high accuracy. In this research, 16 famous bacteria detection panel was

developed and confirmed by qPCR method. Also, it was confirmed that different food matrix had

little adverse effect to the test results. Finally, by developing modified library preparation

method, the total sample preparation time was decreased without loss of accuracy. Also, the

target marker genes were curated to decreased false positives.

Symposia [S18]

Sponsored by International Vaccine Institute

2024 International Meeting of the Microbiological Society of Korea

154 | www.msk.or.kr

S18-1

Salmonella Typhi Vi-Specific Serological Profiling among

Children, Adolescent, and Adults after Typhoid Vaccination

Gippeum Joy Lim1, Ju Yeon Park1, Deok Ryun Kim1, Sushant Sahastrabuddhe1,

Hun Kim2, Manki Song1*, and Jae Seung Yang1*

1International Vaccine Institute

2SK Bioscience

Typhoid fever is still a major public health concern in low and middle-income countries. Vi

polysaccharide vaccines were developed to prevent and control typhoid fever in endemic region

but showed poor immunogenicity in children under 2 years. To overcome the limitations and to

generate a robust immune response against S. Typhi, typhoid conjugate vaccines (TCVs) have

been developed and three TCVs (Typbar TCV®, TYPHBEV®, SKY Typhoid) have been

pre-qualified by World Health Organization. Although anti-Vi IgG has been widely used for

surrogate marker of protection against typhoid fever and primary endpoint of immunogenicity

of TCV for licensure of vaccine, antibody-mediated protective mechanisms are not fully

understood. Previously, in the phase 1 study of Vi-DT conjugate vaccine, a total 144

participants in three different age strata (2-5, 6-17, and 18-45 years) were recruited to assess

safety and immunogenicity of Vi-DT TCV (SKY Typhoid). Participants were immunized with

Vi-DT TCV twice at one-month interval or Vi polysaccharide vaccine with single injection. In this

study, we analyzed samples to seek distinct immune responses among children, adolescent, and

adults after vaccination with Vi-DT TCV or Vi-PS vaccine using systems serology tool, which

includes not only Vi-specific IgG but also antibody function including serum bactericidal

antibody, antibody-dependent (AD) cell phagocytosis, AD complement deposition, Fc receptor

binding, etc. Biophysical profiling of antibodies (isotypes, subclasses, and FcR) were statistically

significant increased after Vi-DT vaccination compared to Vi-PS. Interestingly, anti-Vi total IgG

was not statistically different between children and adult at one month after Vi-DT TCV

vaccination. However, anti-Vi IgG1 and IgG3 were significantly higher in children compared to

adults (P<0.05). Functional activities (ADCP, ADCD, ADNP) were increased after 2nd dose of

TCV, especially in children although comparable anti-Vi IgG were observed post vaccination

between first and second dose. Distinct biomarkers between children and adults would be

helpful to understand immune responses and antibody-mediated protection against typhoid

infection.

www.msk.or.kr | 155

Symposia [S18] : IVI session - Advances in Vaccine Technology

S18-2

Development of Immune Modulating Ionizable Lipids and

Their LNP Formulation for mRNA Vaccines and Therapeutics

Hyukjin Lee

College of Pharmacy, Ewha Womans University

The mRNA-based vaccines and therapeutics have opened a new era of immunization and

disease treatment, with notable successes in combatting infectious diseases and even cancer.

However, optimizing the delivery of mRNA payloads to target cells and modulating the immune

response remains a critical challenge. In this study, we present our research on the development

of immune modulating ionizable lipids and their incorporation into lipid nanoparticles (LNPs) for

enhancing the efficacy and safety of mRNA vaccines and therapeutics. Our research focuses on

designing and synthesizing a novel class of ionizable lipids, characterized by their pH-responsive

charge-switching properties. Combinatorial synthesis of degradable ionizable lipids has been

carried out using amine head groups with different lipid tail structures. These lipids enable

efficient encapsulation of mRNA payloads, provide superior stability, and facilitate endosomal

escape, leading to improved delivery of mRNA to the cytoplasm. Moreover, we have

systematically tuned the physicochemical properties of these lipids to optimize their

immune-modulating effects, tailoring the balance between pro-inflammatory and tolerogenic

responses. The immune modulating ionizable lipids and their LNP formulation described in this

study have the potential to revolutionize the delivery and immunogenicity of mRNA-based

therapies, with broad implications for the prevention and treatment of a wide range of diseases.

2024 International Meeting of the Microbiological Society of Korea

156 | www.msk.or.kr

S18-3

Advancing Vaccine Delivery: Microneedle Array Patches for

Enhanced mRNA Vaccine Stability and Efficacy

Jooyoung Kim

QuadMedicine, Inc.

Vaccination via intramuscular injection with a syringe is accurate and cost-effective but has

significant drawbacks, such as the need for trained medical staff, a cold chain, and large storage

facilities. Microneedle array patches (MAPs) offer a promising alternative, enhancing storage

stability, extending shelf life, and improving user convenience. QuadMedicine has developed

various MAP technologies, including coated, separable, and powder-attached MAPs (P-MAPs),

targeting vaccines for hepatitis B, influenza, and COVID-19. P-MAPs, which attach freeze-dried

vaccine particles to microneedle tips, preserve mRNA integrity and enable faster skin delivery.

This innovative platform is well-suited for rapid responses to public health emergencies,

including future pandemics.

www.msk.or.kr | 157

Symposia [S18] : IVI session - Advances in Vaccine Technology

S18-4

Development of 4-Valent Vaccine Conferring Optimal

Protection against Pore-forming Toxins of

Staphylococcus aureus

Gi-Sub Choi1, Joon-Hwan Ji1, Bok Luel Lee3, and Jin-Han Kang1,2*

1Research Center, CLIPS BnC

2The Vaccine Bio Research Institute, College of Medicine, The Catholic University

3Host Defense Protein Laboratory, College of Pharmacy, Pusan National University

S. aureus is a Gram-positive bacterium that can spread through hospital-acquired or

community-acquired infections. As it is a bacterium that frequently develops antibiotic

resistance, there is a high likelihood of pathogenicity when infecting immunocompromised

individuals or patients with underlying diseases. It can cause diseases such as pneumonia,

bacteremia, and myocarditis. Although many researchers have attempted to develop MRSA

(methicillin resistant S. aureus) vaccines, those that showed promising results in preclinical

trials have failed to demonstrate efficacy in clinical trials. In this study, we developed a vaccine

by selecting toxins that destroy immune cells and blood cells in the human body as antigen

candidates. Among various toxins, we chose Hla, HlgA, LukS, and LukAB as antigen proteins and

used them to create the vaccine. Antigens were obtained using an E. coli expression system and

Ni-NTA chromatography, and humoral immunity was analyzed using various adjuvants. In the

rabbit septic challenge model, survival rate and abscesses in the kidneys were analyzed. The

group that received the vaccine showed a 100% survival rate, and the protective effects of the

antibodies were confirmed using bacterial culture supernatants. In experiments to select the

optimal adjuvant, it was confirmed that the adjuvant combining liposome and MPL increased

total IgG and IgG2a, which is associated with cellular immunity. This multivalent vaccine

provides a novel approach that enables the human immune system to effectively cope with

MRSA by preventing the destruction of immune cells.

Symposia [S19]

2024 International Meeting of the Microbiological Society of Korea

160 | www.msk.or.kr

S19-1

Microbiome Therapeutics: The “Molecule-First” Strategy

for Targeted Interventions

Sang Sun Yoon

1Department of Microbiology and Immunology, Yonsei University College of Medicine

2BioMe Inc.

Recent FDA approvals of Rebyota (2022) and Vowst (2023) mark a pivotal moment in

microbiome therapeutics. However, challenges persist in developing microbial therapeutics

with well-defined Modes of Action (MOA) and moving away from reliance on spores or whole

feces as Drug Substances (DS). To overcome these obstacles, we introduce the “Molecule-First”

strategy. By selecting target molecules for degradation or overproduction, we identify

microbial strains with desired functional capabilities. Our focus lies on trimethylamine N-oxide

(TMAO), a known risk factor for cardiovascular diseases, and butyrate, a beneficial short-chain

fatty acid (SCFA). Through our screening approach, we discovered the strain BM109, a

bacterium of human fecal origin that completely degrades TMAO without generating harmful

byproducts. Oral administration of BM109 significantly reduces plasma TMAO levels, indicating

its potential as a live biotherapeutic for individuals at high-risk cardiovascular disorders.

Additionally, the strain BM107 effectively breaks down Tributyrin, producing three molecules of

butyrate through an active esterase function. Our “Molecule-First” approach enables the

identification and comprehension of MOAs in specific microbes, propelling the development of

targeted and effective therapeutic interventions.

www.msk.or.kr | 161

Symposia [S19] : Bio-Biz Festival

S19-2

Resolving Biology with Scalable, Single Cell

Multiomic Solutions

YuJeong Gho

DAON BioSciences Inc.

Single-cell RNA sequencing (scRNA-seq) offers unparalleled insights into the complexity of

cellular populations by allowing the analysis of gene expression at the individual cell level,

revealing cellular heterogeneity that is often masked in bulk analysis. The 10x Genomics

Chromium System is transforming biological research by enabling high-throughput single-cell

transcriptomics. This innovative platform allows researchers to explore cellular heterogeneity

and gene expression with unprecedented resolution, driving breakthroughs in diverse fields,

including microbiology, oncology, immunology, and developmental biology. This presentation

will highlight the significant impact of the Chromium System on advancing our understanding of

microbial communities, host-pathogen interactions, and the complexity of cellular ecosystems

across various research domains.

2024 International Meeting of the Microbiological Society of Korea

162 | www.msk.or.kr

S19-3

Comprehensive Bioinformatics Solution for Microbial

Genomics, Metagenomics

Kyung-yun Kim

Insilicogen, Inc.

The vast majority of microbes inhabiting our planet remain uncultivated. It is estimated that

less than 1% of all microbes have been characterized in culture. Microbial genomics and

metagenomics approaches are the key to unlocking this treasure of hidden biological

information.

Ever-growing sample volumes demand efficient bioinformatics. Yet the lack of

well-integrated analytics for microbial genomics and metagenome analysis leaves researchers

and organizations with the burden of integrating and maintaining all the required tools for

bioinformatics, statistics, and visualizations to power their research.

Whether one focuses on public health epidemiology, clinical microbiology research, or basic

microbial genomics research, CLC Genomics Workbench Premium provides state-of-the-art

tools for metagenomics and microbiome analysis, and strain typing of bacterial, fungal, and viral

genomes.

CLC Genomics Workbench efficiently handles complex metagenomics data and allows

researchers to reveal important relationships between the microbiome, metagenome and host

by providing streamlined workflows for gene discovery, annotation, taxonomy, and functional

microbiome analysis.

For advanced bioinformatics and genomics analysis of antimicrobial resistance (AMR) genes

and markers, Insilicogen provides epidemiological metagenome analysis tools and microbiome

analysis tools, as well as the necessary databases, to conveniently perform analysis.

www.msk.or.kr | 163

Symposia [S19] : Bio-Biz Festival

S19-4

The Career Path of Microbiologists to

Being a Corporate Researcher

Byung-Yong Kim

R&D Center, Chong Kun Dang (CKD) Healthcare

Microbiologists play a pivotal role in advancing scientific knowledge and driving innovation

across various industries. As aspiring professionals, understanding the career trajectory from

academia to corporate research is crucial. In this presentation, we explore the multifaceted

journey that transforms microbiologists into impactful corporate researchers.

1. Transitioning to Industry: Corporate research environments differ significantly from

academia. Adaptability is key. Microbiologists must learn to navigate interdisciplinary teams,

project management, and industry-specific challenges. Networking plays a vital role. Attend

conferences, engage with industry professionals, and explore internships or collaborative

projects with companies.

2. Skills Beyond the Lab: Beyond technical expertise, corporate researchers need business

acumen. Understanding market trends, regulatory frameworks, and intellectual property

rights is crucial. Communication skills—both written and verbal—are essential. Researchers

must convey complex findings to diverse audiences, including executives, marketers, and

investors.

3. Embracing Interdisciplinary Collaboration: Corporate research often involves cross-

functional teams. Microbiologists collaborate with chemists, engineers, marketers, and legal

experts. Learning to appreciate diverse perspectives and contribute effectively to team

goals is essential.

4. Navigating Career Growth: Corporate researchers can specialize in areas like drug

development, food safety, or biotechnology. Continuous learning and staying updated on

industry trends are vital. Seek mentorship from experienced colleagues and explore

professional development opportunities.

5. Balancing Passion and Practicality: Passion for science drives microbiologists, but practical

considerations matter too. Evaluate company culture, work-life balance, and growth

prospects. Some choose to pursue advanced degrees (e.g., MBA) to enhance their managerial

skills.

In summary, the path from microbiology labs to corporate boardrooms is dynamic and

rewarding. By embracing lifelong learning, networking, and adaptability, microbiologists can

thrive as valuable contributors to corporate research endeavors.

Symposia [S20]

2024 International Meeting of the Microbiological Society of Korea

166 | www.msk.or.kr

S20-1

Biofilm Control by Metazoa in Membrane Systems for

Wastewater Treatment

Jaewoong Jeong, Heewon Byeon, Suyeon Lee, and Seonki Lee*

Department of Environmental Engineering, National Korea Maritime and Ocean University

Biofouling is a natural phenomenon where microorganisms adhere to surfaces and form

biofilms, occurring on most surfaces. However, when biofilms form on membrane surfaces, they

cause decreased permeability, increased chemical cleaning frequency, and higher operational

costs. To date, various physical and chemical methods, as well as bacterial-based strategies

such as quorum sensing inhibition, have been developed to control biofouling in membrane

systems for wastewater treatment. However, in addition to bacteria, diverse eukaryotes are

present in wastewater environments and coexist with bacteria in biofilm layer. However, the

potential applications of metazoans for biofilm control have not been extensively studied. In

this study, we aimed to explore whether metazoans, known for their predatory behavior and

high mobility, could be utilized for biofilm control.

www.msk.or.kr | 167

Symposia [S20] : Microbial Ecology

S20-2

Assessment of Ecological Disturbance through Phenotypic

Dynamics of Groundwater Microbiome

Jin-Kyung Hong1,2, Soo Bin Kim1, and Tae Kwon Lee1*

1Department of Environmental and Energy Engineering, Yonsei University

2now at Department of Environmental and Energy Engineering, Chonnam National University

Interaction between microorganisms and their surrounding environments occur at all

organizational levels. Microorganisms can rapidly switch their phenotypes in response to

environmental fluctuation, leading to community shift governed by distinct assembly

mechanisms. This study aims to develop a comprehensive method for assessing ecological

disturbances in groundwater ecosystems caused by tetrachloroethylene (PCE) contamination,

using flow cytometry (FCM) fingerprinting. We also seek to elucidate the mechanisms of

disturbance through ecological models, including co-occurrence networks and the neutral

community model. FCM revealed significant alterations in the phenotypic structure of

PCE-contaminated groundwater, providing clearer and more direct insights into contamination

impacts compared to taxonomic diversity measures. The disturbed groundwater microbiome

exhibited higher centrality in the co-occurrence network and lower fitness in the neutral

community model, indicating that PCE contamination also drives community-level shifts.

Dechlorinating bacteria and their syntrophic partners were identified as hub-taxa and involved

deterministic assemblage. These taxa also showed positive correlation with disturbance-

indicating phenotypes, suggesting that the metabolic utilization of contaminants is a key factor

driving the disturbance. This study establishes FCM fingerprinting as a simple, robust, and

accurate method for evaluating ecological disturbances, with potential applications in early

warning systems and continuous monitoring of groundwater contamination. The findings

underscore the sensitivity of FCM in detecting phenotypic variations induced by environmental

stressors and highlight its utility in understanding the complex dynamics of microbial

communities in contaminated groundwater ecosystems.

2024 International Meeting of the Microbiological Society of Korea

168 | www.msk.or.kr

S20-3

Isolation of Novel Archaeal Virus Infecting Marine

Ammonia-oxidizing Thaumarchaea

Na-Ri Kim1, Eun-Jung Choi1, So-Jeong Kim2, and Jong-Geol Kim1*

1Department of Life Science, Wonkwang University

2Geologic Environment Research Division, Korea Institute of Geoscience and Mineral Resources

Ammonia-oxidizing archaea (AOA) from the phylum Thaumarchaeota are known to play a

central role in carbon and nitrogen cycling in marine environments, yet research on viruses that

infect these archaea remains largely unexplored. Studies on viruses are crucial for

understanding the abundance, evolution, and carbon recycling of their hosts. Previous

metagenomic studies suggest the presence of lytic viruses among marine archaeal viruses. The

Nitrosopumilus spindle-shaped viruses (NSVs), first reported, has a life cycle that is entirely

different from typical bacteriophages. NSVs suppresses host growth but releases new viruses

through budding rather than lysing the host. Additionally, NSVs is characterized by a very

narrow host range. The recently isolated virus is presumed to be a typical bacteriophage of the

podovirus type, which lyses its host after infection and then releases new viruses. The results

provide new insights into the role of viral predation in shaping microbial communities and their

potential influence on nitrogen and carbon cycling in marine ecosystems.

www.msk.or.kr | 169

Symposia [S20] : Microbial Ecology

S20-4

Application of WGS for

Source Tracking for Antibiotic Resistance (STAR)

Hokyung Song1 and Tatsuya Unno2*

1Department of Environmental Engineering, Chosun University

2Department of Microbiology, Chungbuk National University

The presence of fecal contamination is a strong indicator of antimicrobial resistance (AMR)

proliferation, as fecal bacteria frequently harbor antibiotic resistance genes (ARGs). Microbial

source tracking (MST), an essential method for identifying the origin of fecal contamination, was

conducted using FEAST, a machine-learning model specifically developed for MST. We applied

MST to assess the Miho River, comparing the extent of fecal contamination before and after

significant rainfall. The findings revealed that the river was primarily contaminated by fecal

matter from livestock following the rainfall, implicating the surrounding environment as a

significant contributor to the pollution.

We further employed metagenomic analysis to explore the antibiotic resistome. Using a

custom-developed pipeline, we quantified the abundance of ARGs, identified potential bacterial

hosts, and assessed the mobility of these ARGs. The results indicated that the environments

harbor a diverse array of ARGs, predominantly those associated with plasmids, thereby

positioning these environments as significant sources of ARGs in the wider ecosystem.

Overall, the findings from these studies underscore the significant role of aquatic

environments as reservoirs of antibiotic-resistant bacteria. Although these bacteria may not be

inherently pathogenic, there exists a potential risk for them to transfer ARGs to pathogenic

strains. While current research efforts predominantly focus on AMR surveillance, our results

highlight the urgent need for future research to prioritize strategies aimed at preventing the

spread of AMR across different environmental contexts, given the persistent nature of this

threat.

Symposia [S21]

2024 International Meeting of the Microbiological Society of Korea

172 | www.msk.or.kr

S21-1

The Role of Histone Modifications in Regulating Responses

to External Signals in Candida albicans

Jueun Kim, Yong-Joon Cho, and Jung-Shin Lee*

Department of Molecular Bioscience, Kangwon National University

Precise gene regulation is essential for maintaining cellular homeostasis and enabling

environmental adaptation in living organisms. In eukaryotes, transcriptional regulation is

intricately controlled by chromatin structures and histone modifications. Set1-mediated

methylation of histone H3 at lysine 4 (H3K4) is a well-known marker of active transcription.

However, in the dimorphic pathogenic fungus Candida albicans, deletion of Set1 leads to the

unexpected induction of signal-responsive genes even in the absence of external stimuli. This

study investigates the role of Set1 in the transcriptional regulation of these genes. The results

reveal that during early induction, H3K4 methylation is not required for these genes’ rapid and

robust expression; instead, acetylation of H3K4 plays a key role. In the absence of H3K4

methylation, H3K4 acetylation levels increase significantly, triggering mRNA expression in the

absence of external signals and leading to morphological changes. With prolonged exposure to

induction signals, H3K4 acetylation decreases while H3K4 methylation rises, facilitating

sustained gene expression. These findings suggest that Set1 precisely balances H3K4

methylation and acetylation to regulate the timing and extent of inducible gene transcription.

www.msk.or.kr | 173

Symposia [S21] : Recent Advances in the Molecular Biology of Microbes in Host Environments

S21-2

Dynamic 3D Genome Reorganization in Response to

Environmental Stress

Jae Min Lee†, Seong Ho An

†, and Kyoung-Dong Kim*

Department of Systems Biotechnology, Chung-Ang University

†These authors contributed equally

The three-dimensional (3D) organization of genomes changes dynamically in response to

environmental conditions and can regulate gene expression. Hi-C technology and next-

generation sequencing have revealed hierarchical 3D genome organization in microbes and

higher organisms. By comparing chromatin packing patterns across the genome under normal or

varying conditions, we observed dynamic changes in 3D genome structure in the fungus

Malassezia restricta and the fission yeast Schizosaccharomyces pombe.

Malassezia is one of the most frequently isolated fungal genera from the human skin

microbiome. We observed that the proximal association of the Malassezia 3D genome was

significantly reduced when co-cultured with Staphylococcus epidermidis. In addition, genomic

association was also reduced at proximal distances under low pH conditions due to acetic acid

treatment rather than hydrochloric acid. This finding indicates that bacteria can influence the

3D organization of fungi through acetic acid secretion, affecting chromosomal fidelity and

overall gene expression patterns.

Potential changes in 3D genome organization were also observed in S. pombe. We observed

a genome-wide decrease in the binding affinity of the condensin component Cut14, a key

organizer of the 3D genome, when subjected to iron deficiency in S. pombe, whereas the

cohesin components Rad21 and RNA Pol II were unaffected. As measured by fluorescence

microscopy and mini-chromosome loss assays, we observed a significant increase in the

frequency of chromosome segregation defects upon iron deficiency, which is consistent with

the ChIP-seq results. This study shows that iron deficiency reduces the binding affinity of SMC

complexes genome-wide, which can lead to changes in 3D genomic organization and

chromosome segregation defects.

2024 International Meeting of the Microbiological Society of Korea

174 | www.msk.or.kr

S21-3

The Formylmethionine Ribosome Quality Control Pathway

in Saccharomyces cerevisiae for Cold Adaptation

Cheol-Sang Hwang

Department of Life Sciences, Korea University

In contrast to the long-lasting brief that N-formylmethionine (fMet)-mediated translation is

confined to bacteria or bacteria-descended organelles for initiating protein synthesis, our

recent studies have revealed that eukaryotic cells also synthesize fMet-containing proteins

within the cytosol. Particularly, the expression of Escherichia coli FMT (EcFMT), a

methionyl-tRNA formyltransferase, in budding yeast Saccharomyces cerevisiae cells results in

increased production of N-terminally formylated cytosolic proteins. These proteins can be

degraded via the eukaryotic fMet/N-degron pathway which selectively recognizes the

N-terminal fMet of formylated proteins for degradation. Under conditions of EcFMT expression,

these N-terminally formylated proteins can comprise ~7% of the total detected protein pool.

However, only about 5% of these N-terminal formylated proteins can be eliminated by the

fMet/N-degron pathway. Given that EcFMT expression in yeast cells can convert up to

approximately 70% of yeast Met-tRNAi to fMet-tRNAi, we asked whether eukaryotic cells

retain mechanisms to regulate the production of fMet-bearing polypeptides directly during

translation. Here, I will present the discovery of such a system, termed the eukaryotic

fMet-mediated ribosome quality control (fMet-RQC) pathway and its critical role in cold

adaptation.

www.msk.or.kr | 175

Symposia [S21] : Recent Advances in the Molecular Biology of Microbes in Host Environments

S21-4

MARTX Toxin, the Primary Virulence Factor Produced in

Vibrio vulnificus

Byoung Sik Kim

Department of Food Science and Biotechnology, Ewha Womans University

The MARTX (multifunctional autoprocessing repeats-in-toxin) toxin is a major virulence

factor of Vibrio vulnificus, a pathogen responsible for life-threatening gastrointestinal and

wound infections. This extremely large single polypeptide toxin consists of an amino-terminal

repeats-containing region, multiple cytotoxic/cytopathic effector domains, a cysteine protease

domain, and a carboxyl-terminal repeats-containing region. The excreted MARTX toxin is

believed to form a pore on the host cell plasma membrane through its repeats-containing

regions at the amino- and carboxyl- termini. Subsequently, the pore enables the translocation

of the toxin’s effector domains into the host cell cytosol, causing cytotoxicity/cytopathicity,

and induces cell blebbing, which manifests necrosis ultimately. In this presentation, the

interactions between MARTX toxin and host cells will be elucidated using the Makes Caterpillars

Floppy-like effector domain and ADP-ribosylation factor proteins as an example interaction

pair. Additionally, the evidence supporting the increasing threats posed by MARTX

toxin-mediated infections will be discussed. Finally, the future potential of engineered MARTX

toxin will be explored to provide insights into its possible biomedical applications.

2024 International Meeting of the Microbiological Society of Korea

176 | www.msk.or.kr

S21-5

A Salmonella Type II TA Module Leads to Formation of

Persister Cells inside Host

Soomin Choi and Eun-Jin Lee*

Department of Life Sciences, Korea University

Toxin-antitoxin systems are ubiquitous in most of sequenced bacterial genomes. TA systems

play an important role in bacterial growth and persistence in host or antibiotic-treated

conditions by inhibiting essential cellular processes. TA systems are classified in 8 types

depending on types of antitoxin. Among these, type II TA system is a type of protein-protein TA

module that encodes a toxin protein and a neutralizing antitoxin protein. Interestingly, many of

toxins encode endoribonucleases that cleave RNA in a ribosome- or sequence-dependent

fashion, resulting in translational inhibition. However, it was poorly defined the target of

endoribonuclease toxins. In this study, we identified a HigB-like endoribonuclease toxin that

causes ribosomal RNA cleavage and ribosome disassembly. This would induce Salmonella’s

persistence inside the host and during exposure to antibiotics.

Graduate Students’ Forum

GS4

Co-organized by BET Research Institute, Chung-Ang University &

Institute of Microbiomics, Chung-Ang University

2024 International Meeting of the Microbiological Society of Korea

178 | www.msk.or.kr

GS1-1

Enhancing Gut Health: The Impact of Seed Mucilage

Prebiotics on Infant and Adult Microbiomes

Kanika Mahra1, Vineet Singh1, and Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University

2Department of Integrative Biology, Kyungpook National University

3Next Generation Sequencing (NGS) Core Facility, Kyungpook National University

The gut microbiome, a complex community of microorganisms residing in the gastrointestinal

tract, plays a crucial role in maintaining human health by regulating metabolism, immune

function, and protecting against pathogens. The development of the gut microbiome begins at

birth and continues to evolve, influenced by various factors such as mode of delivery, diet, and

environment, with diet being the most significant factor in shaping this microbial community.

Prebiotics, non-digestible carbohydrate components that promote the growth of beneficial gut

bacteria, are essential in supporting a healthy microbiome. Our study investigates the effects of

new breed of prebiotics derived from seed mucilage on the gut microbiome of both infants and

adults.

In present work, we extracted the seed-mucilage from seeds and examined their prebiotic

properties, focusing on its impact on gut bacterial populations and the production of major

short-chain fatty acids (SCFAs), including butyrate, propionate, and acetate. We performed NGS

for the microbiome data analysis to predict the changes in the microbial community

composition. The findings demonstrated that the prebiotic activity of seed mucilage

significantly increased the abundance of beneficial gut bacteria. Additionally, gas

chromatography (GC) analysis revealed enhanced production of SCFAs, which are critical for gut

health. This study highlights the potential of seed mucilage as an effective prebiotic to improve

gut microbiome composition.

www.msk.or.kr | 179

Graduate Students’ Forum

GS1-2

Gut Microbiome Modulation by Cellulose-degrading

Bacteria for Enhancing Gut Health and Growth Performance

in Mouse Model

Gi Beom Keum, Sheena Kim, Jinok Kwak, Hyunok Doo, Yejin Choi, Juyoun Kang,

Haram Kim, Yeongjae Chae, and Hyeun Bum Kim*

Department of Animal Biotechnology, Dankook University

Applying bacteria capable of utilizing cellulose, which monogastric animals cannot digest, can

improve the host’s gut health and microbiota structure. In this study, we evaluated the

potential of cellulose-degrading bacteria isolated from rabbits to improve gut health in mice.

Strains were isolated from 11 rabbit feces and selected through in vitro tests for

Carboxymethylcellulose (CMC), acid and bile resistance. Their genetic safety was confirmed

through whole genome sequencing (WGS). The selected bacteria were administered to mice

along with the indigestible fiber fructooligosaccharides, and their performance was evaluated

through the following experiments: H&E staining and tight junction protein expression

assessment of ileum, fecal 16S rRNA gene sequencing. After the in vitro tests and WGS analysis,

2 strains were finally selected: Bacillus halotolerans (B. halotolerans) and Bacillus

amyloliquefaciens (B. amyloliquefaciens). In the mouse in vivo experiment, no histological

abnormalities were observed in the ileum. Additionally, the group that consumed B.

amyloliquefaciens showed a significant increase in the expression of tight junction proteins

compared to the control group, and the gut microbiota had a significant abundance of

fiber-fermenting and short-chain fatty acid-producing bacteria such as Prevotella and Alistipes.

This study suggests that a synbiotic formulation of cellulose-degrading bacteria and prebiotics

could enhance gut health and microbiota structure.

2024 International Meeting of the Microbiological Society of Korea

180 | www.msk.or.kr

GS1-3

Polyhydroxyalkanoate Production in Halophilic Marine

Bacteria and Assessing Bioplastics Biodegradability

Sondor Ganbat1, Dariimaa Ganbat1, Yujin Son2, Ji Yeong Park1, Joo Young Yang1,

Dong-Woo Lee3, Sung Tae Kim4, Seong-Bo Kim5, and Sang-Jae Lee1*

1Department of Bioscience and Research Center for Extremophiles and Marine Microbiology, Silla

University

2Department of Integrative Biotechnology (IBT), Yonsei University

3Department of Biotechnology, Yonsei University

4Department of Nanoscience and Engineering, Inje University

5Bio-Living Engineering Major, Global Leaders College, Yonsei University

Polyhydroxyalkanoates (PHAs) are a group of biopolymers with various applications and

excellent biodegradability, but the high cost of production has prevented their use. To reduce

this cost, there is a prospect for strains with a high PHA production and the ability to grow in

low-cost medium as well as easy cell lysis to PHA recovery. In this context, this work is aimed to

conducting a bioprospection of bacteria isolated from marine environments in South Korea for

the potential production of PHA. A diverse group of bacteria were isolated and screened by

Sudan Black B and Nile Red A staining. A total of 27 potent PHA-producing bacteria belonging

to the genera Halomonas spp., and Cobetia spp. were characterized. PHA granules in the cells

were visualized using a confocal microscope. The presence of a PHA synthase gene (phaC) was

confirmed by PCR. Gas chromatography analysis was performed for confirmation of the PHA

fractions with extracted polymers were identified as polyhydroxybutyrate (PHB). Preliminary

analysis revealed that the selected marine bacteria have a high salinity tolerance and PHA

accumulation activity. The complete genome sequence of Halomonas spp. revealed the

presence of the extracellular PHA depolymerase gene in their chromosomes, and their

PHA-degrading activity was confirmed. The results of this study suggest a high potential for

PHA production, as well as the biodegradability and recycling of PHA by halophilic bacteria

isolated from the marine environment.

www.msk.or.kr | 181

Graduate Students’ Forum

GS1-4

Metagenomic Profiling of Age-related Changes

in Facial Skin Microbiome

Ikwhan Kim1, Da-Ryung Jung2, YeonGyun Jung3, Yoon Soo Cho4, and Jae-Ho Shin1,2,5*

1Department of Integrative Biology, Kyungpook National University

2Department of Applied Biosciences, Kyungpook National University

3Burn Institute, Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital,

Hallym University College of Medicine

4Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital,

Hallym University College of Medicine

5NGS Core Facility, Kyungpook National University

Skin aging is a complex process beyond cosmetic concerns, increasing the risk of skin diseases

by making the skin more susceptible to infection and reducing its healing ability. In maintaining

skin health and homeostasis, the skin microbiome plays a crucial role. Previous studies have

shown that the skin microbiome changes significantly over the course of life, depending on age,

gender, ethnicity and specific areas of the skin. Given the complex interactions between these

factors, detailed profiling of the skin microbiome is essential to understand its role in skin aging.

In this study, we used shotgun metagenomic sequencing to profile the facial skin microbiome of

Koreans, with a focus on comparing differences between different age groups. Our analysis

revealed that bacterial taxonomy and function were significantly affected by age. With

significant decrease in Cutibacterium acnes, bacterial diversity increased in older group. In

addition, younger group had a significantly higher expression of pantothenate and CoA

biosynthesis, which has the potential to maintain a healthy skin microbiome. As the first study

to profile the facial skin microbiome of Koreans using shotgun metagenomic sequencing, we

expect this study to help develop highly specific and effective anti-aging strategies unique to

Koreans of different ages.

2024 International Meeting of the Microbiological Society of Korea

182 | www.msk.or.kr

GS1-5

Gut Microbiome in Patients with Parkinson’s Disease Varies

by Premotor Rapid-eye Movement Sleep Behavior

Disorders Status

Jae-Yun Lee1, Sungyang Jo2, Sun Ju Chung2*, and Jin-Woo Bae1*

1Department of Life and Nanopharmaceutical Sciences, Kyung Hee University

2Department of Neurology, Asan Medical Center, University of Ulsan College of Medicine

Parkinson’s disease (PD) is the second most prevalent neurodegenerative disorder worldwide.

Emerging evidence suggests that the pathogenesis of PD in some patients may originate from

the gastrointestinal tract, thereby highlighting the potential association between the gut

microbiome and PD pathogenesis. Our research aimed to explore the characteristics of the gut

microbiome based on the presence or absence of premotor rapid-eye movement sleep behavior

disorder (premotor RBD), a potential marker of the body-first subtype of PD. We analyzed 16S

rRNA gene amplicon and shotgun metagenomic sequencing data from patients with PD, either

with or without premotor RBD, and healthy controls, and revealed a distinct gut microbiome

composition in the patients with premotor RBD compared to the other groups independent of

disease stage. In contrast, the gut microbiome composition of the patients without premotor

RBD more closely resembled that of healthy controls and varied depending on the disease

stage. Furthermore, we found distinct taxonomic and functional gut microbiome features in the

patients with premotor RBD, potentially associated with enteric alpha-synuclein aggregation.

These findings suggest possible mechanistic associations between the gut microbiome and PD

pathogenesis, offering insights that could drive the development of novel therapeutic targets.

www.msk.or.kr | 183

Graduate Students’ Forum

GS1-6

Epigallocatechin-3-gallate Ameliorates Inflammatory Bowel

Disease through Gut Microbiota-dependent Modulation of

NLRP6 Inflammasome Signaling

Soo-Jeong Lee and Ju-Hoon Lee*

Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research

Institute of Agriculture and Life Sciences, Center for Food and Bioconvergence, Seoul National University

Host-microbiome co-evolution drives homeostasis and influences disease susceptibility,

including inflammatory bowel disease (IBD). However, the regulatory principles governing the

integrated intestinal host microenvironment remain poorly understood. Several studies have

demonstrated that the gut microbiota may contribute to the development of IBD.

Epigallocatechin-3-gallate (EGCG), a major bioactive constituent of green tea, is known to

alleviate IBD symptoms. However, the mechanism by which the gut microbiota influences the

effectiveness of EGCG in attenuating IBD is unclear. While inflammasome signaling is crucial in

host-microbiota interactions, it remains unknown whether EGCG alters the gut microbiota and

modulates inflammasome activity. Here, we demonstrate that EGCG administration and altered

microbiota-associated metabolites shape the host-microbiome interface by co-modulating

NLRP6 inflammasome signaling and downstream colonic IL-18 and IL-1β secretion. This study

provides the first evidence that EGCG ameliorates colonic inflammation in a gut

microbiota-dependent manner through modulation of the NLRP6 inflammasome. Our findings

offer novel insights into EGCG-mediated remission of IBD and suggest that EGCG may serve as

a potential modulator of the gut microbiota, providing new strategies for preventing and

treating IBD by targeting the NLRP6 inflammasome.

2024 International Meeting of the Microbiological Society of Korea

184 | www.msk.or.kr

GS1-7

CRISPR-guided Platforms for Functional Research and

Engineering of Pseudomonas aeruginosa Phages

Shin-Yae Choi, Hee-Won Bae, and You-Hee Cho*

Program of Biopharmaceutical Science, College of Pharmacy and Institute of Pharmaceutical Sciences,

CHA University

Pseudomonas aeruginosa is one of the most notorious bacterial pathogens that is frequently

identified in multidrug-resistant infections. These infections are associated with significant

morbidity and health care expenditures and thus need for alternative and/or supplementary

therapeutic modalities such as phage therapy. The therapeutic potential of phage therapy

could be boosted by gene function research and genome engineering to increase the benefits or

decrease the drawbacks of the natural phages. Despite their importance and versatility in

research and applications, most of the phage genes are poorly characterized mainly due to the

lack of the systematic platforms for gene knock-down and engineering in P. aeruginosa. In this

proof-of-concept study, we exploit the Streptococcus pyogenes Cas9 (SpCas9) system to

generate two platforms in P. aeruginosa strain PAO1: one is for CRISPR interference (CRISPRi)

gene knock-down based on dead SpCas9 (dSpCas9) and the other is for simplified genome

engineering by coupling SpCas9 and the Red recombination system of the coliphage λ (λRed).

The platform cells were created by chromosomal integration of the genes for either dSpCas9 or

SpCas9 and λRed under an inducible promoter. The gene(s) for the single guide RNA and the

editing template have been cloned in a multicopy plasmid. Topics discussed will include the

results from arrayed genome-wide CRISPRi study of the anti-twitching phage, D3112 and

creation of the fluorescence-tagged phage particles from anti-CRISPR phage, MP29.

www.msk.or.kr | 185

Graduate Students’ Forum

GS1-8

Evaluation of Potential Probiotics Candidates with

Anti-allergic Effect to Alleviate Mast Cell Degranulation and

Food Allergy Symptoms in Balb/c Mouse Model

So-Yeon Jin1,3,4, Jihye Yang1,3,4, and Ju-Hoon Lee1,2,3,4*

1Department of Agricultural Biotechnology, Seoul National University

2Department of Food and Animal Biotechnology, Seoul National University

3Research Institute of Agriculture and Life Sciences, Seoul National University

4Center for Food and Bioconvergence, Seoul National University

Allergic diseases, such as food allergy, are influenced by the diversity and distribution of gut

microbiota, and probiotics have been demonstrated to alleviate allergic symptoms by altering

the cytokines produced by T helper cells. This study was designed to evaluate the anti-allergic

effects of probiotics on levels of mast cell degranulation and pro-inflammatory cytokines

associated with allergic symptoms in RBL-2H3 mast cells. To screen anti-allergic activities, 30

probiotics were isolated from fermented foods and human feces, co-cultured with RBL-2H3

mast cells, and β-hexosaminidase release was evaluated. As a result, out of 30 strains, 7 strains

showed remarkable inhibitory effects upon RBL-2H3 mast cell degranulation. Next, the

real-time PCR showed that 4 strains significantly reduced pro-inflammatory cytokine IL-4,

IL-13, and TNF-α levels in DNP-IgE/BSA sensitized RBL-2H3 mast cells. Furthermore, among 4

strains, Lactiplantibacillus plantarum SLpl116 also showed anti-allergic effect in ovalbumin-

induced food allergy Balb/c model. Following 7 weeks of oral administration, SLpl116

significantly decreased allergic symptoms including diarrhea occurrence, change in rectal

temperature, serum immunoglobulin levels in FA mouse. In conclusion, due to their anti-allergic

properties, these strains expected to have the potential to be used as candidate probiotic

strains to prevent food allergy symptoms.

2024 International Meeting of the Microbiological Society of Korea

186 | www.msk.or.kr

GS1-9

Halotolerant Beneficial Bacteria Alleviate Salinity Stress in

Maize by Modulating Soil Microbiome

Tino Bashizi1, Minsoo Jeong1, and Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University

2Department of Integrative Biology, Kyungpook National University

3NGS Core Facility, Kyungpook National University

This study, conducted at the Agricultural Research Center of Kyungpook National University

in Gunwi, South Korea, evaluated the effectiveness of halotolerant beneficial bacteria obtained

from the maize rhizosphere in the Democratic Republic of the Congo. The primary objective was

to enhance maize growth in saline conditions by manipulating the soil microbiome. Maize plants

were inoculated with two distinct bacterial strains individually, and their impact was compared

to conventional chemical fertilizers. Results demonstrated significant enhancements in various

maize growth parameters, including plant height, leaf area, and biomass. Additionally, these

bacterial strains positively influenced both maize phenotype and soil microbiome composition.

This dual functionality showcases their potential as eco-friendly biofertilizers, offering

substantial benefits for plant growth and soil health. The findings suggest practical applications

in agriculture to address soil salinity challenges, providing a promising approach for sustainable

farming practices amidst harsh environmental conditions. This research emphasizes the vital

role of halotolerant beneficial bacteria in fostering sustainable agricultural development, but

also their capacity to improve crop resilience and productivity in saline soils.

www.msk.or.kr | 187

Graduate Students’ Forum

GS1-10

Understanding the Influence of a Foliicolous Lichen on the

Endophytic Mycobiome Using a Metabarcoding Approach

Yunhyeok Jang1, Yoonjoo Noh1, Yehyeon Cha1, Jung-Jae Woo2,

Jae-Seoun Hur2, and Seung-Yoon Oh1*

1Department of Biology and Microbiology, Changwon National University

2Korean Lichen Research Institute, Sunchon National University

Foliicolous lichens, which live on plant leaves, form symbiotic relationships with the leaves of

vascular plants, using them as substrates. On the other hand, endophytic fungi reside within

plant tissues without causing symptoms and are known to enhance plant adaptability to

environmental stresses by producing secondary metabolites. Although research on the

diversity of endophytic fungi and lichens has been actively conducted, research on their

relationships is lacking. This study investigated the diversity of endophytic mycobiome in trees

associated with the foliicolous lichen (Strigula multiformis). For two tree species (Litsea

japonica and Machilus thunbergii), this study compares endophytic mycobiome in tree leaves

with and without a foliicolous lichen. A metabarcoding analysis showed significant differences in

endophytic mycobiome. In L. japonica, all alpha diversity measures showed significant

differences, while in M. thunbergii, no significant differences were observed. Beta diversity

analysis indicated significant differences based on host species and lichen colonization types.

Ascomycota was the dominant phylum in both host species and colonization types. The

presence of S. multiformis influences endophytic mycobiome, providing insights into the

ecological roles of foliicolous lichens and their impact on endophytic fungal communities.

[This work was supported by a grant from the Korea Basic Science Institute (National Research

Facilities and Equipment Center) funded by the Ministry of Education (2023R1A6C101B022).]

2024 International Meeting of the Microbiological Society of Korea

188 | www.msk.or.kr

GS1-11

Gut Microbiome–Creatine Synergistic Effects

against Obesity

Su-Won Jeong, Jeong Eun Han, and Jin-Woo Bae*

Department of Biology, Kyung Hee University

Gut microbiome is influenced by dietary intake and associated with obesity development.

Here, we investigated whether the anti-obesity effects of creatine, a widely-used exercise

supplement, are mediated by gut microbiome. Using a high-fat-diet-induced mouse obesity

model, we demonstrated that creatine supplementation reduced body-weight gain and

improved insulin resistance. Creatine-modified cecal microbiota transplantation reproduced

these effects. Cecal 16S-rRNA sequencing revealed that creatine supplementation increased

the abundance of taxa with anti-obesity effects, such as Lactobacillus species, Akkermansia

muciniphila, Parabacteroides goldsteinii, and Bacteroides uniformis, suggesting that their high

abundance enhances the beneficial effects of creatine. Notably, B. uniformis administration had

anti-obesity effects, protected from high-fat-diet-induced muscle wasting, and promoted

brown-adipose-tissue function, which further enhanced the creatine-mediated anti-obesity

effects. Our study indicates that creatine has anti-obesity effects, which are amplified by

changes in gut microbiome, and suggests that regulation of gut microbiome may provide

therapeutic strategies for treating obesity and other metabolic diseases.

[This research was supported by the Bio & Medical Technology Development Program of the

National Research Foundation (NRF) & funded by the Korean government (MSIT) (NRF-2022

M3A9F3082331).]

www.msk.or.kr | 189

Graduate Students’ Forum

GS2-1

Identification and Validation of Small Molecule Inhibitor

Targeting Biofilm Formation Regulator CsgD

Yunjeong Kim, Jihye Park, and Byoung Sik Kim*

Department of Food Science and Biotechnology, College of Engineering, Ewha Womans University

Enterohemorrhagic Escherichia coli (EHEC) is a foodborne pathogen causing abdominal pain

and bloody diarrhea. Traditional antibiotics, while effectively inhibit the bacterial growth, often

lead to antibiotic resistance and enhanced production of Shiga-toxin. This study aims to find

alternatives to manage EHEC virulence without affecting bacterial growth. Biofilm formation of

EHEC relies on Curli fimbriae produced by csgBA operon, which is activated by the

transcriptional regulator CsgD. AlphaFold-based structural analysis revealed that the CsgD may

have a putative ligand-binding pocket as like to its structural homologue VpsT in Vibrio

cholerae. Accordingly, a CsgD-activated luminescence reporter system was developed to

identify CsgD inhibitors. Screening 8,201 small molecules revealed a chemical A4 as the

potential CsgD inhibitor. Biofilm formation assay demonstrated that A4 suppresses CsgD not

only in EHEC but also in other pathogens, which contain a CsgD homologue, such as Salmonella

Typhimurium, Vibrio vulnificus and Listeria monocytogenes. Through the Congo Red binding

assay, we confirmed that A4 inhibits the Curli production. In addition, A4 substantially reduced

the amount of CsgD in EHEC, suggesting that the chemical inhibits the expression of CsgD gene

or affects the stability of CsgD protein. In conclusion, A4 is the novel inhibitor which probably

binds to the CsgD, offering a sustainable anti-biofilm strategy for EHEC and other pathogens.

2024 International Meeting of the Microbiological Society of Korea

190 | www.msk.or.kr

GS2-2

Reassessing the Diversity of Penicillium in Marine

Environments with the Proposal of Twelve New Species

Ji Seon Kim, Wonjun Lee, and Young Woon Lim*

School of Biological Sciences and Institute of Biodiversity, Seoul National University

Penicillium has gained significant attention following the discovery of penicillin. It has been

intensively researched in various fields, such as industry and medicine. The taxonomic field has

faced challenges as non-taxonomists have claimed new species without following the standard

method. In the last decade, we have isolated Penicillium strains from various marine

environments in South Korea. Through an integrative study of taxonomy and phylogenetics, we

found that the diversity of Penicillium in marine environments has been significantly

underestimated, proposing 12 new species. Furthermore, using precise phylogenetic analysis,

we reevaluated reports of Penicillium in marine environments from previous literature and

discovered a significant number of incorrect information. This re-evaluation updates the

current list of Marine Fungi and offers a more precise picture of the diversity of Penicillium in

marine environments. We emphasize the significance of precise identification and provide a

framework for identifying and exploiting Penicillium from various marine environments as a

significant fungal resource.

www.msk.or.kr | 191

Graduate Students’ Forum

GS2-3

Accumulated Peptidoglycan Turnover Products Lead to

Excessive Outer Membrane Vesicle Production in

Salmonella Typhimurium 14028S

Jihye Yang, Yongjun Son, Yerim Park, and Woojun Park*

Laboratory of Molecular Environmental Microbiology, Department of Environmental Sciences and

Ecological Engineering, Korea University

Gram-negative Salmonella Typhimurium 14028S exhibited different sensitivity against

varying β-lactam antibiotics due to their distinct selectivity for thirteen penicillin-binding

proteins (PBPs). Under non-lethal concentration of cephalexin (LEX), the quantity of outer

membrane vesicles (OMVs) using a lipophilic dye was higher (~2.3 fold) than that under the

other non-lethal β-lactam antibiotics. Our electron microscopy observed that Salmonella

Typhimurium 14028S was morphologically shifted into a filamentous bacterium secreting many

OMVs under LEX conditions due to inhibiting DD-transpeptidase PBP3, an essential protein for

survival based on BOCILLINTM-fluorescent profiling. Our proteomic analyses using outer- (OM)

and inner-membrane (IM) regions revealed that amidase (AmiA) and lytic transglycosylase (RlpA

and YceG) in OM, belonging to peptidoglycan (PG) recycling pathway, were significantly

increased, but the abundance of muropeptide (PG turnover products) transporters (OppDE) in

IM was reduced under LEX stress, indicating that accumulated PG strands might lead to

excessive OMVs production. Our findings suggest that accumulating PG turnover products in

the periplasm by inhibiting PBP3 under LEX could release OMVs to mitigate cell envelop stress.

2024 International Meeting of the Microbiological Society of Korea

192 | www.msk.or.kr

GS2-4

The Potential of C-di-AMP as a Druggable Target for

Reversing β-Lactam Resistance in MRSA

Ji‐Hoon Kim1, Yunmi Lee2, Inseo Kim1, JuOae Chang1, Subin Hong1, and Wonsik Lee1*

1School of Pharmacy, Sungkyunkwan University

2Antibacterial Resistance Laboratory, Institut Pasteur Korea

Cyclic-di-adenosine monophosphate (c-di-AMP) has been implicated in various bacterial

physiologies, including osmoregulation and maintaining cell wall integrity. Particularly, the levels

of c-di-AMP are known to be related to disruptions in bacterial cell wall homeostasis; however,

the mechanisms are not fully understood. We identified a compound that effectively inhibited

the growth of S. aureus USA300 only in the presence of methicillin, whereas neither the

compound nor methicillin alone had observable effects. This synergistic effect was also

confirmed in other MRSA strains, such as MW2 and clinical isolates. Furthermore, we showed

that the synergistic is specific to β-lactam antibiotics, including cephalosporins, suggesting

that the mechanism of action is related to the crosslinking of peptidoglycan (PG) biogenesis in

MRSA. In our effort to elucidate the mechanism of action of the compound, we found mutations

in the gdpP of the resistant mutants. The gdpP was inactivated in all the resistant mutants,

which would lead to an increase in cellular c-di-AMP levels. Moreover, we demonstrated that the

overexpression of gdpP led to a decrease in resistance to methicillin in MRSA. Overall, our

results demonstrate that intracellular c-di-AMP is important for maintaining the crosslink of PG

in S. aureus, highlighting c-di-AMP as a novel therapeutic target to overcome the resistance to

β-lactams.

www.msk.or.kr | 193

Graduate Students’ Forum

GS2-5

The Impact of Gut Microbiome on Type 2 Diabetes

Improvement: Insights from Roux-en-Y Reconstruction

after Gastrectomy

GyuDae Lee1, Min-Ji Kim1, HeeJoo Hwang3, JiYeon Park2, and Jae-Ho Shin1,3,4*

1Department of Applied Biosciences, Kyungpook National University

2Department of Surgery, School of Medicine, Kyungpook National University, Kyungpook National

University Chilgok Hospital

3Department of Integrative Biotechnology, Kyungpook National University

4NGS Core Facility, Kyungpook National University

Roux-en-Y reconstruction not only treats severe obesity but also effectively ameliorates

type-2 diabetes (T2D). We hypothesized that alterations in the gut microbiome might play a

pivotal role in T2D improvement following surgery. To test this, we studied non-obese T2D

patients with early-stage gastric cancer.

Three months post-surgery, all 12 patients showed improvements in Type 2 Diabetes (T2D),

evidenced by decreased HbA1c levels and significantly increased secretion of gut hormones

GLP-1 and PYY. Shotgun metagenomic sequencing revealed substantial alterations in the gut

microbiome, with notable increases in Bacteroides spp., Alistipes spp., Lactobacillus spp.,

Limosilactobacillus spp., and Veillonella spp. Pathways related to short-chain fatty acid (SCFA)

biosynthesis, crucial for stimulating GLP-1 and PYY, were enhanced postoperatively. GC-FID

analysis confirmed elevated SCFA levels post-surgery. Metagenome-assembled genomes

(MAGs) identified functional genes in the increased species, supporting their role in SCFA

production. These findings suggest a potential mechanistic link between gut microbiome

changes and T2D improvement following Roux-en-Y reconstruction.

Overall, our study revealed that changes in the gut microbiota induced by Roux-en-Y

reconstruction can lead to changes in the metabolites including SCFA produced in the gut,

which in turn stimulate the secretion of gut hormones, potentially contributing to the

improvement of T2D.

2024 International Meeting of the Microbiological Society of Korea

194 | www.msk.or.kr

GS2-6

Modulation of Gut Microbiota and Metabolites by Laminarin

during In Vitro Human Fecal Fermentation: Bacteroides as a

Potential Key Decomposer

Juhui Jang, Seongok Kim, Wonjune Lee, and Hakdong Shin*

Department of Food Science & Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong

University

Laminarin, a storage polysaccharide derived from brown algae, is noted for its antitumor,

anticoagulant, and blood pressure-reducing properties. This study investigates the influence of

laminarin on gut microbiota and determines its metabolic profiling to assess its potential health

benefits in humans. Fecal samples from healthy adults (n = 20) were collected and cultured

using in vitro fecal incubation under anaerobic conditions. To deconvolve interindividual

variability, participants were classified into two distinct enterotypes: 14 subjects with a

Bacteroides-dominant type and 6 subjects with a Prevotella-dominant type. Using 16S

rRNA-based sequencing, laminarin-treated group showed the lower microbial diversity, with

community structures significantly varying between the control and laminarin treated group.

The genera Bacteroides and Faecalibacterium were predominant in the laminarin-treated group

compared to the control. Untargeted metabolomic analysis revealed higher levels of

metabolites such as short-chain fatty acid (SCFA), GABA, and arginine in the laminarin-treated

group, along with enriched genes for TCA cycle. These findings suggest that laminarin could

serve as a prebiotic by affecting gut microbial structure and promoting the production of

metabolites beneficial to human health.

www.msk.or.kr | 195

Graduate Students’ Forum

GS2-7

Sweet Food, Bitter Life: Glucose-mediated Acidic Stress

Compromises the Membrane Integrity in Clinical

Acinetobacter baumannii

Jihyeon Min, Yerim Park, Wonjae Kim, Yongjun Son, Bitnara Kim, and Woojun Park*

Department of Environmental Science and Ecological Engineering, Korea University

Multidrug-resistant Acinetobacter baumannii, an opportunistic pathogen in the urinary tract,

metabolizes glucose into gluconic acid, leading to acidification of the growth medium.

Glucose-supplemented environments with elevated acidity cause significant surface changes in

A. baumannii NCCP 16011, including the formation of extracellular polymeric substances (EPS)

and biofilms. The blurring of the outer membrane and the expansion of the periplasmic region

under high nutrient stress were verified using transmission electron microscopy (TEM) analysis.

The upregulations of genes related to outer membrane proteins and penicillin-binding proteins

(PBPs) including transpeptidase and glycosyltransferase were revealed using transcriptomic

analysis and Bocillin fluorescence labeling assay, respectively. However, the excessive presence

of PBPs and the reduction in transpeptidase activity hindered cross-linking between the outer

membrane and peptidoglycan (PG), leading to the excessive production of outer membrane

vesicles (OMVs). Proteomic analysis revealed that the bacterial OMVs contain β-lactamase

enzymes such as BlaOXA-75 and the autotransporter adhesin protein Ata, which could contribute

to cell aggregation. Collectively, our results indicate that acidic stress caused by high nutrient

conditions induces membrane alterations and OMV production, potentially enhancing cell

aggregation and the transport of antibiotic-resistance genes.

2024 International Meeting of the Microbiological Society of Korea

196 | www.msk.or.kr

GS2-8

A Periplasmic Protein Modulates the Proteolysis of

Peptidoglycan Hydrolases to Maintain Cell Wall

Homeostasis in Escherichia coli

Sohee Park1, Wook-Jong Jeon1, Yeseul Lee2, Chae Lim Lim1, Han Byeol Oh3,

Eunyeong Lee1, Oh Hyun Kwon1, Bumhan Ryu4, Sung-il Yoon3,

Jeong Min Chung2, and Hongbaek Cho1*

1Department of Biological Sciences, College of Natural Sciences, Sungkyunkwan University

2Department of Biotechnology, The Catholic University of Korea

3Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University

4Research Solution Center, Institute for Basic Science

Most bacterial cells are encapsulated by a peptidoglycan (PG) molecule that functions as the

cell wall. In Gram-negative bacteria, the activity of DD-endopeptidases that cleave 4-3 peptide

crosslinks of PG is essential for PG assembly, and maintaining an optimal level of

DD-endopeptidase activity is critical for expanding PG without damaging its integrity. In

Escherichia coli, the levels of major DD-endopeptidases, MepS and MepH, and a lytic

transglycosylase MltD, are subject to proteolytic control by the periplasmic protease Prc and its

outer membrane adaptor NlpI, but how the turnover of these enzymes is regulated has

remained unclear. In this study, we discovered a periplasmic protein, BipP (formerly YhjJ), that

negatively controls the NlpI-Prc system. Further analyses indicate that BipP exerts this control

by interacting with NlpI and inhibiting its substrate recognition in response to low

DD-endopeptidase activity, providing insight into the homeostatic control of PG hydrolysis and

cell wall expansion.

www.msk.or.kr | 197

Graduate Students’ Forum

GS2-9

Optimal Selection of Bacteria-mediated Delivery to

Enhance Therapeutic Efficacy in Breast Cancer Models

Jam-Eon Park1,2, Seung-Hyon Choi1, Ju Huck Lee1, Jung-Sook Lee1,

Ji-Sun Kim1*, and Seung-Hwan Park1*

1Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology

2Department of Molecular Medicine, Chonnam National University

Bacteria-mediated cancer therapy is an innovative approach that exploits the tumor-

targeting ability of bacteria to deliver anti-cancer drugs directly to tumors. Cytolysin A (ClyA),

a key anti-cancer agent carried by these bacteria, has been demonstrated to be effective in

treating colorectal cancer; however, it has shown minimal efficacy in breast cancer. To address

the limitations of breast cancer therapy using ClyA, we aimed to develop Clostridium perfringens

enterotoxin (CPE)-expressing bacteria targeting claudin-4, which is overexpressed in breast

cancer. In mouse tumor models, ClyA showed elevated therapeutic efficacy in CT26 colon

tumors, whereas CPE exhibited high efficacy in 4T1 breast tumors. Additionally, tumors treated

with ClyA and CPE showed distinct differences in immune cell infiltration patterns. ClyA

enhanced the overall number of immune-related cells in CT26 tumors, whereas CPE decreased

the number of neutrophils but increased the proportion of other immune cells in 4T1 tumors.

Neutrophil-related G-CSF expression was markedly reduced in 4T1 tumors treated with CPE

compared with that in CT26 tumors. These results demonstrate distinct mechanistic

differences in anti-cancer effects between ClyA and CPE in CT26 and 4T1 tumors. Our study

emphasizes that the optimal selection of bacteria-mediated delivery based on cancer type lead

to more effective therapeutic outcomes.

2024 International Meeting of the Microbiological Society of Korea

198 | www.msk.or.kr

GS2-10

Characterization of a Plasmid-dependent Phage for

Controlling Antibiotic Resistance Gene-carrying Bacteria in

the Gut Microbiota

Geunsoo Jung, Jinwoo Kim, and Hakdong Shin*

Department of Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University

The emergence and spread of antimicrobial resistance in bacteria pose a serious global threat

to public health. One of the major drivers of this spread is the transfer of antimicrobial

resistance genes (ARGs) via plasmid DNA through bacterial conjugation. In this study, we

isolated a novel phage, PDP46, which specifically infects bacteria carrying the conjugative

plasmid IncF that encodes antibiotic resistance genes. This phage requires the IncF plasmid for

infection, enabling it to specifically target and inhibit bacterial strains involved in plasmid

transfer. Genomic analysis revealed that this phage is lytic and lacks any virulence factors,

indicating its potential as a safe biocontrol agent. Additionally, we assessed the phage’s

potential as a gut modulator using the in vitro fecal incubation model. Human stool samples

inoculated with E. coli carrying antibiotic-resistance plasmids were treated with the phage, and

the relative abundance of bacterial taxa was evaluated. Phage treatment selectively inhibited

the growth of target bacteria harboring conjugative plasmids without disrupting overall

microbial diversity in the samples. Our findings suggest that phage PDP46 can effectively

reduce the spread of ARGs by selectively eliminating plasmid-harboring donor bacteria and also

has the potential to serve as a gut modulator by specifically controlling pathogenic strains in

the gut.

www.msk.or.kr | 199

Graduate Students’ Forum

GS2-11

Interplay Between Vaginal Microbiome and High-risk HPV in

Cervical Dysplasia

Da-Ryung Jung1, Ye Seul Choi2, Minsoo Jeong1, Vineet Singh1, Se Young Jeon3,

Hyung Soo Han2, Gun Oh Chong3, and Jae-Ho Shin1,4*

1Department of Applied Biosciences, Kyungpook National University

2Department of Biomedical Science, Graduate School, Kyungpook National University

3Department of Obstetrics and Gynecology, Kyungpook National University Chilgok Hospital

4NGS Core Facility, Kyungpook National University

Cervical cancer (CC) is commonly caused by human papillomavirus (HPV) infections

worldwide, with particularly oncogenic high-risk types such as HPV 16 and 18. The vaginal

microbiome (VM) plays an important role in infection and progression of HPV, but still

underexplored. This study investigated the interplay between specific high-risk HPV (hrHPV)

and the VM’s structure and function, and how these interactions influence the development and

outcomes of HPV-related cervical conditions. Using shotgun metagenomic sequencing, we

analyzed the VM across different hrHPV and the severity of cervical lesions. Our results

demonstrate notable differences in the VM associated with HPV 16 and 18 compared to other

hrHPV. VM in CC patients infected with HPV 16 and 18 showed distinct microbial profiles with

increased inflammatory and immune responses, while other hrHPV were linked to pathway

involved in cellular metabolism and hormonal signaling. Our research indicates that HPV 16/18

infections, associated with specific bacterial enrichments, may worsen lesion severity and

promote oncogenesis, underscoring the need for further study on their complex, bidirectional

interactions with the vaginal microbiome in CC development.

2024 International Meeting of the Microbiological Society of Korea

200 | www.msk.or.kr

GS2-12

Fructose-responsive Regulation by FruR in

Faecalibacterium prausnitzii for Its Intestinal Colonization

HyeLim Choi and Yeong-Jae Seok*

School of Biological Sciences and Institute of Microbiology, Seoul National University

Faecalibacterium prausnitzii is a dominant member of healthy human gut microbiota and its

abundance exhibits strong positive correlation with fecal fructose levels. F. prausnitzii relies on

the phosphoenolpyuruvate (PEP): carbohydrate phosphotransferase system to import

fructose, simultaneously phosphorylating it to fructose 1-phosphate (F1P). Here, we show that

FruR, a DeoR family transcriptional regulator, interacts with F1P and the histidine-containing

phosphocarrier protein HPr2 for the sophisticated transcriptional regulation of the fructose

(fru) operon. FruR can facilitate the RNA polymerase binding to the fru promoter only in the

presence of both HPr2(Ser-P) and F1P. The formation of FruR-HPr2(Ser-P)-F1P complex leads

to stronger binding to specific sequence motifs in the fru promoter compared to Apo-FruR.

These binding sequences, located between the -35 and -10 elements and downstream of the

transcription start site, are crucial for appropriate binding and transcriptional initiation of the

FruR-RNA polymerase complex. F1P induces a structural modification in FruR, resulting in an

increased binding affinity of its C-terminal domain to HPr2 and enhanced dynamics of its

N-terminal DNA-binding domain. This regulatory mechanism of FruR activity by F1P and HPr2

provides insight into how fructose levels impact the predominance of F. prausnitzii in the human

intestine.

www.msk.or.kr | 201

Graduate Students’ Forum

GS3-1

Therapeutic Potential of Engineered Endolysin CD27L_EAD

against Clostridioides difficile Infection

Youngjin Cho1 and Hyunjin Yoon1,2*

1Department of Molecular Science Technology, Ajou University

2Advanced College of Bio-convergence Engineering, Ajou University

Clostridioides difficile infection (CDI) presents a significant health challenge, with current

antibiotic treatments exacerbating the emergence of antibiotic-resistant bacteria and

recurrent CDI. This study investigated the potential of the engineered endolysin CD27L_EAD as

an alternative therapeutic agent for CDI. CD27L_EAD, which was modified from bacteriophage

peptidoglycan hydrolase phiCD27_gp34 (Accession number YP_002290910.1) showed

enhanced bacteriolytic activity across various conditions. Metagenomic analysis demonstrated

that CD27L_EAD effectively reduced C. difficile populations in vitro without disrupting the

overall gut microbiota composition. Additionally, the combination of CD27L_EAD and

vancomycin exhibited synergistic bactericidal effects against C. difficile. The analysis confirmed

that CD27L_EAD treatment maintained gut microbiota diversity and community structure,

unlike traditional antibiotics, which often lead to dysbiosis. The results support CD27L_EAD as

a promising candidate for CDI treatment, providing a targeted approach that minimizes

collateral damage to beneficial gut bacteria. This research underscores the potential of

endolysins in addressing antibiotic resistance and highlights the importance of developing

microbiota-sparing therapeutics.

2024 International Meeting of the Microbiological Society of Korea

202 | www.msk.or.kr

GS3-2

Investigation of Inflammation Alleviation in an IBD Mouse

Model Using the Butyrate-producing Strain and Tributyrin

Hyuna Sung and Sang Sun Yoon*

Department of Microbiology and Immunology, Yonsei University College of Medicine

Inflammatory Bowel Disease (IBD), containing ulcerative colitis (UC) and Crohn’s disease (CD),

is chronic inflammation that occurs throughout the gastrointestinal (GI) tract. Recently

increasing Inflammatory Bowel Disease (IBD) patients, we need to find a new approach. Not only

the use of chemical treatment but also the usage of probiotic treatment can expect better

therapeutic effects.

Finding safe and efficiently decomposing TB bacteria and isolating that best breaks down TB

in human feces. Additionally, we assessed the bacteria’s anti-inflammatory effect in dextran

sodium sulfate (DSS)-induced mice with TB.

The isolated strain demonstrated anti-inflammatory properties and promoted the recovery

of intestinal epithelial cells. Furthermore, when treated with TB, a more rapid recovery was

observed. Compared to the control group, the group treated with both the isolated strain and

TB showed the fastest recovery in terms of weight gain.

The strain isolated from human feces, when administered with TB in a DSS model, exhibited

enhanced anti-inflammatory properties through butyrate, effectively alleviating inflammation.

Therefore, this isolated strain holds promise as a novel probiotic treatment for IBD.

www.msk.or.kr | 203

Graduate Students’ Forum

GS3-3

Metagenomic, Untargeted Metabolomic, and

Physicochemical Insights of Korean Fermented Foods

Amani Sliti1, Eskindir Getachew Fentie1, and Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University

2NGS Core Facility, Kyungpook National University

3Department of Integrative Biotechnology, Kyungpook National University

Fermented foods are highly valued for their nutritional value and health benefits, including

probiotic, antioxidant, and anti-inflammatory properties. Microbes play a central role in the

fermentation process, enhancing food organoleptic, nutritional, and functional profiles. This

study investigated the physicochemical characteristics, bacterial communities, and metabolic

profiles of five types of kimchi, soybean paste, and salted fish collected from Daegu, South

Korea, using Illumina MiSeq sequencing targeting the V4 region of the 16S rRNA gene and

LC-Q-TOF-MS for the analysis of untargeted metabolites. pH levels varied across the samples:

3.98 to 5.29 in kimchi, 4.62 to 5.60 in soybean paste, and 5.95 to 6.46 in salted fish. Salinity

was highest in soybean paste (3.99%), followed by salted squid (1.88%), and kimchi samples

(1.01% to 1.85%). Sequencing showed Firmicutes dominance, with Proteobacteria more

prevalent in bitter lettuce, soybean, and perilla leaf kimchi. Salinity influenced microbial

community diversity more than pH (Mantel test, p < 0.05). Therefore, the annotation of

microbial functional and metabolic profiles indicated significant roles in fermentation, aerobic

chemoheterotrophy, and the synthesis pathways for carbohydrates, amino acids, carotenoids,

and riboflavin. These findings provide valuable insights into the microbial diversity of probiotic

foods and their potential health benefits.

2024 International Meeting of the Microbiological Society of Korea

204 | www.msk.or.kr

GS3-4

Developing Oral Vaccines against Foot-and-Mouth Disease

Virus Using Saccharomyces cerevisiae

Ngoc My Tieu Le1, Kum-Kang So2, Geon-min Kim1, and Dae-Hyuk Kim1,2*

1Department of Bioactive Material Sciences, Jeonbuk National University

2Institute for Molecular Biology and Genetics, Department of Molecular Biology, Jeonbuk National

University

The development of a vaccine to protect against Foot-and-Mouth Disease (FMD) is an urgent

need to ensure the safe and efficient production of cloven-hoofed animals. To overcome the

drawbacks of the traditional vaccines, our studies focused on producing an oral vaccine against

FMDV. The gene that encodes a structural precursor protein P1 region of FMDV, which contains

four structural capsid proteins [VP0 (VP4 and VP2), VP3, and VP1] followed by a translational

“ribosomal skipping” sequence consisting of 2A and protease 3C, was codon-optimized and

chemically synthesized. We engineered S. cerevisiae 2805 strain to express structural proteins

of FMDV and produce virus-like particles. Since VP1 is the major immunogenic protein of FMDV

due to containing both T and B cell epitopes which induce a cellular immune response as well as

sufficient levels of neutralizing antibodies for protection against infection, fused proteins in

yeast was applied to enhance VP1 expression. For eliciting the mucosal immune response in

mice by oral vaccination, we constructed VP1 sequence with M cell targeting Co1 ligand or

displayed VP1 on the yeast cell wall. All constructs were verified by western analysis or electron

microscopy. The recombinant proteins showed a significant immune response to the FMDV

structural epitope protein, which opens the possibility of an oral FMDV vaccine.

www.msk.or.kr | 205

Graduate Students’ Forum

GS3-5

Potential Role of malR-malABC Regulon in Inducing

Virulence of Staphylococcus pseudintermedius during

Canine Otitis Externa

Ji Hyun Lim1, Gi Yong Lee2, Ji Heon Park1, and Soo-Jin Yang1*

1Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for

Veterinary Science, Seoul National University

2Department of Molecular Microbiology and Immunology, Keck School of Medicine of University of

Southern California, Los Angeles CA, USA

Staphylococcus pseudintermedius is an opportunistic pathogen in dogs and is recognized as

a significant threat to human health. Previously, it has been identified that the maltose-specific

phosphotransferase system (PTS) in S. pseudintermedius, encoded by malABC operon, is

involved in enhanced virulence during skin and soft tissue infections (SSTIs) in dogs. The

putative malABC operon was positively regulated by the malR located upstream of malA. In this

study, the role of the malR-malABC regulon in enhanced virulence of S. pseudintermedius during

SSTIs was evaluated by generating two isogenic mutants of malR and malABC (ΔmalR and Δ

malABC, respectively). The two mutant strains exhibited similar profiles of growth to the

wild-type strain. However, the two mutant strains displayed increasing pH of 8.0–8.5 during

stationary growth phase, while the wild-type strain showed approximately pH 5.0, indicating

altered ability to utilize maltose in the two mutants. Moreover, deletion of malABC and malR

resulted in significant decrease in adhesion to canine epidermal keratinocytes (CPEK) cells.

Furthermore, the two mutant strains displayed increased susceptibility to canine-derived

cathelicidin, K9CATH (4.5 µg/ml), both in the presence and absence of maltose. These results

demonstrate that the putative malR-malABC regulon is involved in the enhanced virulence of S.

pseudintermedius in response to maltose during canine SSTIs.

2024 International Meeting of the Microbiological Society of Korea

206 | www.msk.or.kr

GS3-6

Computational Analysis of E4 Gene Evolution in Human

Adenovirus Species D

Chanhee Lee, Anyeseu Park, and Jeong Yoon Lee*

The Laboratory of Viromics and Evolution, Korea Zoonosis Research Institute, Jeonbuk National University

Adenovirus (Ad) is a widespread pathogen that can infect a variety of animals and humans.

Human adenovirus (HAdV) can lead to severe infections, particularly in individuals with

compromised immune systems. To date, more than 110 types of HAdV have been categorized

into seven species, ranging from A to G, with the majority classified under human adenovirus

species D (HAdV-D). In HAdV-D, the primary factor for the emergence of new adenovirus types

is homologous recombination between viral genes that influence virus tropism or help evade

the host immune system. The E4 gene, which comprises seven open reading frames (ORFs), is

involved in both the regulation of host cell metabolism and viral gene replication. Despite

extensive researches, the specific functions of each ORF remain unclear. Our updated data

indicate that ORF2, ORF3 and ORF4 are regions with relatively high mutation rates compared to

other ORFs in the E4 gene, as revealed by in silico comparative analysis. Furthermore, we have

successfully visualized highly mutated segments, previously undetectable at the DNA level,

using a powerful amino acid sequence analysis tool known as proteotyping. Our study has

demonstrated the involvement of the E4 gene in the evolution of HAdV and provided precise

sequence information for the E4 gene, laying the groundwork for future researches.

www.msk.or.kr | 207

Graduate Students’ Forum

GS3-7

Endolysin PHICD111_20024_EAD as a Potent Therapeutic

Agent against Clostridioides difficile Infection

Jeongseok Park1 and Hyunjin Yoon1,2*

1Department of Molecular Science Technology, Ajou University

2Advanced College of Bio-convergence Engineering, Ajou University

Antibiotic-induced disruption of the human gut microbiome promotes the proliferation of

Clostridioides difficile, leading to C. difficile infection (CDI). Recent studies have revealed the

limitations of antibiotics against CDI, prompting interests in alternative treatments such as

endolysin therapy. Two phage endolysins, CD27L and PHICD111_20024 were selected for their

inhibitory properties from a pool of 24 candidates and were tested for their antibacterial

activity against C. difficile under diverse in vitro conditions. Then CD27L_EAD and

PHICD111_20024_EAD lacking the cell wall-binding domain were tested. Both truncated

endolysins showed greater lytic activities than their full-length forms. Intriguingly, despite the

high sequence similarity between them, PHICD111_20024_EAD demonstrated greater stability

than CD27L_EAD under high salt concentrations and in medium broth conditions. Both

truncated endolysins were proposed to have a similar structure, possessing a Zn-binding site.

However, the lytic activity of PHICD111_20024_EAD was easily compromised by adding EDTA,

indicating different Zn-binding affinities between them. When their inhibitory activities were

tested in an in vitro CDI model using fecal microbiota, PHICD111_20024_EAD exhibited a

greater inhibitory effect on C. difficile than CD27L_EAD. These results underscore the potential

of the endolysin variant PHICD111_20024_EAD as a treatment option against CDI.

2024 International Meeting of the Microbiological Society of Korea

208 | www.msk.or.kr

GS3-8

Genome Editing with Small Prime Editors in Bacteria

Seong Hyeok Ma1,2,3, Junho Cho1,2,3, and Sang Sun Yoon1,2,3*

1Department of Microbiology and Immunology, Yonsei University College of Medicine

2Graduate School of Medical Science, Yonsei University College of Medicine

3Brain Korea 21 Project, Yonsei University College of Medicine

Prime editing is a revolutionary genome editing technique that enables precise modifications

to DNA sequences without the requirement for double strand breaks or donor DNA templates.

Traditional genome editing related to CRIPSR Cas system relies on plasmids for gene

manipulation, but this approach has limitations in those various factors such as promoters,

selection markers, and origins need to be optimized for each strain. In addition, the large size of

components for genome editing makes it challenging to apply other methods than plasmids. To

overcome limitations associated with plasmid-based methods in genetic engineering, we have

first adopted CRISPR prime editing utilizing the small or split prime editors. Our study focuses

on establishing prime editing with small and split prime editors in E. coli. we optimized the codon

and expression conditions for the PE and tested its activity using plasmid. Additionally, we

explored the efficiency split sites in PE for high efficiency. As a preliminary proof of concept, we

targeted the lacZ and KanR gene, facilitating easy detection through blue-white selection and

positive selection with correct editing. Our results demonstrate that the small or split PE

functions successful in E. coli. Furthermore, we suggest the potential to deliver the necessary

components in forms other than DNA for prime editing.

www.msk.or.kr | 209

Graduate Students’ Forum

GS3-9

Induced Dysbiosis to Resident Soil Microbial Communities

Improves Plant Health by Enabling Exotic Microbial Agents

to Prevail in the Rhizosphere

Yohannes Ebabuye Andargie1 and Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University

2Department of Integrative Biology, Kyungpook National University

3Next Generation Sequencing (NGS) Core Facility, Kyungpook National University

Resident rhizosphere microbiome could potentially defend plants from pathogenic microbes

as well as from beneficial exotic microbes through a strong microbial network shield. To

penetrate into the system, we induced a dysbiosis to the resident soil microbiome through

controlled moist-heat treatments at 45°C for 45 min and at 105°C for 15 min. The first heat

treatment resulted in partial modifications to cucumber rhizosphere microbiome after the

introduction of exotic microbes but it could not enhance plant health significantly. However,

the heat treatment at 105°C significantly enhanced cucumber growth and health through

significant shifts in the rhizosphere’s bacterial and fungal alpha diversity (P<0.01) and beta

diversity (P<0.01). These shifts led to the differential abundance of beneficial microbes like

Trichoderma and Penicillium in the heat-treated sets. The microbial diversity shift has also been

extended. On the other hand, the microbiome diversity stayed stable in the non-heat-treated

sets after the introduction of exotic microbes across all the treatments. This study highlights

the complex and interconnected network of the resident soil microbiome as a strong defense

mechanism that prevents beneficial exotic microbes from altering the rhizosphere microbiome

to enhance plant health. Thus, other dysbiosis factors are essential for enabling exotic

beneficial microbes to successfully penetrate, integrate and function within the system.

2024 International Meeting of the Microbiological Society of Korea

210 | www.msk.or.kr

GS3-10

The Diversity and Functional Characterization of Indigenous

Bradyrhizobium Isolates in Korea

Hyorim Choi and Jun Heo*

Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development

Administration

The genus Bradyrhizobium is known as legume symbiont that has a biological nitrogen

fixation and root nodulation. Despite numerous studies conducted worldwide on diversity,

genetic characteristics, and phylogenetic analysis, there has been a noticeable lack of research

on these topics in Korea. To analysis diversity of Bradyrhizobium in Korea, we used

approximately 150 strains deposited in the Korean Agricultural Culture Collection (KACC) since

1997. These strains were analyzed for their physiological, biochemical, and genetic diversity.

We performed re-identification using phylogenetic molecular markers to accurately identify

species. Comparing the reported species, 16 topologically distinct groups were recognized.

Whole-genome sequencing was performed on 12 strains distinguished by their isolation source

or phylogenetic distinction. Furthermore, we conducted the comparative genomic analysis with

the closely related type strains and revealed differences in several functional gene ontology

categories. Additionally, the presence of genes associated with root nodulation (nod gene

cluster) and nitrogen fixation (nif gene cluster) was confirmed. This study broadens the

knowledge of the diversity and functionality of Bradyrhizobium strains distributed in Korea. It

enhances our ecological understanding and builds a foundation for future research.

www.msk.or.kr | 211

Graduate Students’ Forum

GS3-11

Structural Investigation of the Docking Domain Assembly

from trans-AT Polyketide Synthases

Se-Young Son1, Da-Woon Bae1, Eunji Kim2, Bo-Gyeong Jeong1,

Myeong-Yeon Kim1, So-Yeon Youn1, Soojung Yi3, Gyeongmin Kim4,

Ji-Sook Hahn4, Nam Ki Lee3, Yeo Joon Yoon2*, and Sun-Shin Cha1*

1Department of Chemistry & Nanoscience, Ewha Womans University

2Natural Products Research Institute, College of Pharmacy, Seoul National University

3Department of Chemistry, Seoul National University

4School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University

Docking domains (DDs) located at the C- and N-termini of polypeptides play a crucial role in

directing the assembly of polyketide synthases (PKSs), which are multienzyme complexes. Here,

we determined the crystal structure of the C-terminal DD (CDDMlnB) and N-terminal DD (NDDMlnC)

from macrolactin trans-AT PKS, which provides the first atomic view of a DD complex fused to

a functional enzyme. Interface analyses of the CDDMlnB/NDDMlnC complex revealed the molecular

intricacies in the core section underpinning precise DD assembly. Additionally, circular dichroism

and steady-state kinetics demonstrated that the formation of the CDDMlnB/NDDMlnC complex had

no influence on the structural and functional fidelity of the fusion partner, AmpC EC2. This

inspired us to apply the CDDMlnB/NDDMlnC assembly for metabolon engineering. Indeed, DD

assembly induced the formation of a complex between 4-coumarate-CoA ligase and chalcone

synthase, which is involved in flavonoid biosynthesis, leading to a remarkable increase in

naringenin production in vitro.

2024 International Meeting of the Microbiological Society of Korea

212 | www.msk.or.kr

GS3-12

Replicating the Human Gut: An In Vitro Co-culture System

for Host and Anaerobic Microbial Interaction

Haneol Yang1, Chan-Hyeok Park1, Hana Jo1, Ye Seul Son2,

Naeun Son2, Mi-Young Son2,3, and Doo-Sang Park1,3*

1Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience

and Biotechnology (KRIBB)

2Stem Cell Research Convergence Center, KRIBB

3KRIBB School of Bioscience, Korea University of Science and Technology (UST)

Anaerobic gut bacteria are crucial for human health, continuously interacting with the host.

Due to their oxygen sensitivity, it is essential to use an appropriate in vitro intestinal epithelium

model that better mimics the human intestine. In this study, a transwell co-culture model, which

enabled the culture of anaerobes with functional human intestinal epithelial cells (hIECs) in an

anaerobic workstation, was developed and validated to study the interaction between bacteria

and intestinal epithelial cells, compared to the Caco-2 cell model. The hIECs remained viable for

up to 12 h, performing slightly better than Caco-2 cells in this system. Lactiplantibacillus

plantarum and Bifidobacterium longum grew 2 and 6.2 times better, respectively, in the hIECs

compared to Caco-2 cells. In contrast, the viability of Clostridium difficile decreased more in

hIECs, whereas Akkermansia muciniphila was better maintained in hIECs, up to 12 h. As

metabolic products, L. plantarum produced lactate and acetate, B. longum produced lactate,

acetate, and butyrate, and C. difficile and A. muciniphila produced acetate and butyrate,

respectively. Unlike the Caco-2 cell model, where glucose was primarily converted to lactate,

the hIECs model was found to better simulate the human intestinal environment. Gene

expression analysis also revealed changes in the expression of intestinal and tight junction

markers, as well as inflammatory factors, when bacteria were treated with the hIECs.

www.msk.or.kr | 213

Graduate Students’ Forum

GS4-1

Control of Lysogeny Formation in Escherichia coli Using

CRISPR-Cas12f1-Armed Synthetic λ Bacteriophages

Ho Joung Lee, Song Hee Jeong, and Sang Jun Lee*

Department of Systems Biotechnology and Institute of Microbiomics, Chung-Ang University

The host cell surface receptors primarily determine the host specificity of bacteriophages.

Integrating cas genes and guide RNAs into phage genomes could intensify their host specificity

and control effects. In this study, we developed a temperate bacteriophage λ model equipped

with the CRISPR-Cas12f1 system, which can detect the presence of target genes in Escherichia

coli host cells. We demonstrated that synthetic λ phages carrying Cas12f1-sgRNA can prevent

lysogen formation. Building on our previous finding that 3’-end-truncated sgRNA eliminates the

mismatch tolerance of the CRISPR-Cas12f1 system, we showed that synthetic phages with

Cas12f1 and truncated sgRNA can discriminate single-nucleotide differences in the host

genome and determine whether the host cell undergoes complete lysis or lysogeny formation.

These results indicate that CRISPR-Cas12f1-armed synthetic bacteriophages can be used to

specifically regulate microbial hosts by recognizing target DNA sequences with high resolution.

2024 International Meeting of the Microbiological Society of Korea

214 | www.msk.or.kr

GS4-2

Anti-prion System in Saccharomyces cerevisiae: Innate

Immunity to Inside-the-Cell Risk

Sia Han1 and Moonil Son1,2,3*

1Department of Integrated Biological Science, Pusan National University

2Department of Microbiology, Pusan National University

3Microbiological Resource Research Institute Pusan National University

Prions are infectious proteins consisting of only of proteins. Prion conversion occurs when a

prion-forming protein, normally functional, misfolded and later converted its structure to a

prion form.

Prions have been found in a variety of organisms. Among them, Yeast and their prions have

been used as a model system to study its pathological effect on the cell.

Yeast [PSI+] prion is filamentous amyloids of Sup35p whose normal function is in translation

termination. The conversion of Sup35 to Prion form results in read-through of stop codon. For

this reason, this [PSI+] and most other prions are obviously detrimental to host cell. Unlike other

infectious agents, these yeast prions can occur at any time in the cell in the presence of

prion-forming genes.

In S. cerevisiae, anti-prion systems deal with pathogenic amyloid yeast prions by (1) blocking

their generation, (2) curing most variants as they arise, and (3) limiting the pathogenicity of

prion variants that do arise and propagate.

In this study, we identified possible anti-prion components (JJJ1, RPL6B, TSR3) through

screening using yeast knockout collection. As a result of prion induction by overproducing the

Sup35 prion domain resulted in more than 10-fold increase in prion generation compared to

WT. However, restoring the genes did not reduce prion production. This showed that the genes

prevented the generation of prions but did not affect their propagation, and based on this

result, we will further investigate the effect of overproduction of each gene on the [PSI+]

generation.

www.msk.or.kr | 215

Graduate Students’ Forum

GS4-3

Comparison of Microbial Diversity Applied to Amplicon

Sequencing Variant and High-throughput Cultivation in

Lakes of Maritime Antarctica

Jiyoung Yoon1,2, Hanbyul Lee1, Suhyun Kim2, Sun-Yong Ha1, Yeongcheol Han1,

Ki-Tae Park1, Soyeon Kim1, Jang-Cheon Cho2, and Ok-Sun Kim1*

1Division of Life Sciences, Korea Polar Research Institute

2Department of Biological Sciences and Bioengineering, Inha University

Antarctica presents an extreme environment for microbes, characterized by low

temperatures, seasonal variation in solar radiation, and limited nutrient availability. Despite

being microbe-driven ecosystems with simple food chains and low biodiversity, there are very

few studies on the microbial ecology of Antarctic lakes. This study aims to understand the

microbial ecosystem of these lakes, focusing on both the water column and sediment. We

conducted microbial community analysis on the 16S rRNA V3-V4 region using samples from the

water and sediment of five lakes on King George Island. Additionally, high-throughput

dilution-to-extinction culturing was performed on water samples. Microbial community analysis

revealed significant differences in bacterial composition both between the lakes and between

sample types. In the water column, Bacteroidota, Actinobacteria, and Proteobacteria were the

most abundant, in that order. In contrast, the sediments were dominated by Actinobacteriota,

Firmicutes, and Proteobacteria, demonstrating distinct microbial community structures across

different habitats. HTC microbial growth was detected in approximately 5% of 960 inoculated

wells. Phylogenetic analyses of 16S rRNA genes indicated that HTC isolates belonged to

CL500-29_marine_group and Polymorphobacter. These findings provide valuable insights into

the microbial community structure that plays a crucial role in Antarctic lakes ecosystems. This

study enhances our understanding of the relationship among various factors shaping these

unique habitats and their specific microbial characteristics.

2024 International Meeting of the Microbiological Society of Korea

216 | www.msk.or.kr

GS4-4

Mannitol Metabolism as a Key Factor in the Pathogenicity of

Vibrio vulnificus Biotype 1C

Joon-Gi Kwon and Ju-Hoon Lee*

Department of Agricultural Biotechnology, Seoul National University

Vibrio vulnificus is a pathogen commonly found in estuarine waters and various seafood. It

has three biotypes, with biotype 1 being responsible for most human infections. Biotype 1 is

further divided into two genotypes: a clinical genotype (1C) and an environmental genotype

(1E). The specific reasons for their distinct characteristics were unclear, prompting a

comparative genomic analysis through pangenome analysis of complete genomes. Our study

identified that only biotype 1C possesses the mannitol operon gene (mtlADR) necessary for

mannitol metabolism. Experimental validation showed that biotype 1C can metabolize mannitol

in an M9 minimal medium supplemented with 0.5% mannitol, while 1E cannot. RNA-Seq analysis

of biotype 1C wild type (WT) and mtlA/D double mutants under LB and LB broth supplemented

with mannitol (LBM) conditions revealed increased expression of lysine decarboxylase (LDC)

and ornithine decarboxylase (ODC) in WT under LBM conditions. These genes are known to

facilitate bacterial adaptation to low pH and resistance to H2O2. Experimental results showed

that mutants lacking LDC and ODC were more sensitive to H2O2, demonstrating reduced growth

in H2O2-containing media. This suggests that LDC and ODC are crucial for survival under

oxidative stress, a key feature of biotype 1C associated with human infections. KEGG analysis

based on RNA-Seq data indicated shifts in the expression of flagellar genes under LBM

conditions, potentially affecting bacterial motility and biofilm formation. Experiments

demonstrated that mtlA/D mutants exhibited higher swimming motility and lower biofilm

formation compared to WT in LBM conditions. This suggests that the presence of mtlADR genes

in WT might reduce swimming motility, promoting biofilm formation, as reduced movement

allows bacteria to settle and form stable biofilms. This adaptability may enhance survival in the

gastrointestinal tract. Overall, these findings indicate a strong correlation between primary

septicemia and biotype 1C. The ability to metabolize mannitol may enhance the survival and

biofilm-forming capabilities of biotype 1C, facilitating its adaptation to gastrointestinal

environments and contributing to its pathogenicity in humans.

www.msk.or.kr | 217

Graduate Students’ Forum

GS4-5

Selenium Nanoparticles Synthesized by Lactobacillus

plantarum as a Gram-positive Selective Bactericidal Agent

Gyeong-min Kim and Kwang-sun Kim*

Department of Chemistry, Pusan National University

Antimicrobial resistance is a significant health challenge, and the use of bioactive

element-derived nanoparticles as a potential antibiotic alternative has increased. The aim of

this study was to synthesize selenium nanoparticles (SeNPs) with selective bactericidal activity

against Gram-positive bacteria using cell-free supernatants from Lactobacillus plantarum (LP).

The synthesized LP-SeNPs were characterized in UV-visible spectrophotometry, dynamic light

scattering (DLS), and X-ray Diffraction (XRD) patterns. Antibacterial activity against both

Gram-negative and Gram-positive bacteria was evaluated by the microbroth dilution method,

followed by time-kill curve analysis and cell viability assays. The synthesized LP-SeNPs were

150 nm in size and had spherical morphology. Unlike other biosynthesized SeNPs reported,

LP-SeNPs showed selective bactericidal activity against Gram-positive bacteria, especially to

methicillin-resistant Staphylococcus aureus (MRSA) (MIC=0.5 μg/ml). Additional synergistic

activity assays of LP-SeNPs against antibiotics revealed that LP-SeNPs were strongly effective

against cell wall antibiotics. Therefore, our newly synthesized LP-SeNPs could serve as

potential non-hazardous bactericidal NPs against Gram-positive bacteria strains.

2024 International Meeting of the Microbiological Society of Korea

218 | www.msk.or.kr

GS4-6

Microbial Precise Genome Editing Using Truncated sgRNA

and Paired Cas9-NG Nickase

Song Hee Jeong, Ho Joung Lee, and Sang Jun Lee*

Department of Systems Biotechnology, Chung-Ang University

The CRISPR-Cas system, which was discovered as an adaptive immune system in prokaryotes,

has become powerful genome editing tool in many organisms. Occasionally, cleavage occurs at

undesired sites in the genome, posing a significant obstacle to precise editing. The paired

nickase system, which utilizes CRISPR-Cas nickase and a pair of guide RNAs, offers a substantial

advantage in reducing off-target effects by doubling the length of the sequence recognizing

the target. In this study, we utilized the Cas9-NG nickase with 5′-NG as the PAM sequence,

which reduced PAM sequence constraints and enabled the generation of paired nicks at desired

genomic loci. We performed a systematic investigation into the double nick formation sites and

the design of donor DNA within a microbial model system. Our results revealed that precise

single-nucleotide-level editing was achievable on target DNA sequences hybridized with

maximally truncated dual single-guide RNAs (sgRNAs). This study provides important insights

into future research directions for the development and optimization of nickase-derived

genome editing technologies.

www.msk.or.kr | 219

Graduate Students’ Forum

GS4-7

Pangenome Analysis-based Real-Time PCR for Rapid

Quantification of 19 Live Probiotics in Real Food

Yunjeong Kim1,2,3, Joon-Gi Kwon1,2,3, Jonathan Lim1,2,3, and Ju-Hoon Lee1,2,3,4*

1Department of Agricultural Biotechnology, Seoul National University

2Research Institute of Agriculture and Life Sciences, Seoul National University

3Center for Food and Bioconvergence, Seoul National University

4Department of Food and Animal Biotechnology, Seoul National University

Recent clinical trials have highlighted potential probiotic of strain mismatch and

contamination. We identified species-specific genes via pan-genome analysis for precise

probiotic detection to develop a real-time PCR method for rapid identification and

quantification. In selecting target genes, pan-genome analysis utilized more than 4,345

complete genome sequences. Validating these genes across various bacterial genera with

BLASTN, we then designed and tested species-specific primer and probe sets using single plex,

crosscheck, and multiplex PCR (triplicates) in both conventional and real-time methods. Both

PCR methods successfully differentiated species using specific primer and probe sets. The

standard curve results displayed primer slopes between -3.00 to -3.50, with R² value minimum

0.991.

For food application accuracy validation, we introduced a model with strains in sterile milk,

comparing real-time PCR to direct counting. Using these curves, real-time PCR and viable cell

counts were compared using the Mann-Whitney U test, revealing no significant differences.

Moreover, we introduced propidium monoazide (PMA) dye in DNA extraction process to detect

only live cell. This new approach demonstrates its capability to replace plate counting method

which has limitation, ensuring the safety of probiotics in food for human health with accuracy

and rapidity.

2024 International Meeting of the Microbiological Society of Korea

220 | www.msk.or.kr

GS4-8

Three Ribosomal Subunit Proteins as Candidates for

Anti-prion Components in Saccharomyces cerevisiae

Min Suk Jang1 and Moonil Son1,2,3*

1Department of Integrated Biological Science

2Department of Microbiology

3Microbiological Resource Research Institute, Pusan National University

Prion is an infectious protein, mostly replicating self-propagatable amyloid. Saccharomyces

cerevisiae is known as an occasional host of at least 10 prion such as [PSI+] (prion form of

translation termination factor Sup35/eRF3), [URE3] (prion form of the nitrogen catabolic

regulator Ure2p), and [PIN+] (functionally unknown prion of Rnq1p). So, yeast has also evolved

an array of anti-prion systems targeting these prions. The components of the system are

working constantly in normal cells, blocking prion formation and propagation. To complement

previous genetics-based screening that studied these systems, additional screening to find

possible anti-[PSI+] prion components was performed using the yeast knockout collection. The

three ribosomal subunit proteins (RPS18A, RPL24B, and RPL16A) were screened and the effect

of the absence of these genes on the frequency of prion formation was further investigated.

When prion was induced in the deletion mutants, the frequency of prion formation was

increased 3- and 6- fold compared to when induced in WT cells. Further work should include

whether the restoration of each gene by mating, cytoduction and plasmid transformation can

eliminate prion arising in absence of each. This study will confirm that the screened factors were

indeed an anti-[PSI+] component working in normal WT cells, such as the human innate immune

system.

www.msk.or.kr | 221

Graduate Students’ Forum

GS4-9

A Method Evaluation for Analyzing Microbial Communities

in Ice Cores of Styx Glacier, Antarctica

Subin Lee1,2, Minkyung Kim1, Yeongcheol Han3, Chung Yeon Hwang2, and Ok-Sun Kim1*

1Division of Life Sciences, Korea Polar Research Institute

2School of Earth and Environmental Sciences, Seoul National University

3Division of Glacial Environment Research, Korea Polar Research Institute

Glaciers, despite holding over 70% of the Earth’s freshwater, have remained largely

unexplored by microbiologists due to the limitation of sample collection, low microbial biomass,

and the susceptibility to contamination. To understand microbial communities in these

challenging environments, which require unconventional methods unlike traditional

environmental microbial community analyses, we evaluated four distinct approaches to identify

the most effective method: 1) Direct 16S rRNA gene-based PCR amplification of raw samples 2)

Nested PCR with raw samples using various combinations of primers 3) PCR after cell

concentration using FACS (Fluorescence-Activated Cell Sorting), and 4) manual DNA extraction

after FACS-based cell concentration, followed by PCR amplification. After thorough evaluation,

we determined that method 4 was the most effective approach for our samples. We employed

this approach, combining FACS-based cell concentration, phenol-chloroform DNA extraction,

and high-fidelity PCR amplification, to analyze the microbial communities of Styx Glacier,

Antarctica. We anticipate this method could be a valuable tool for investigating communities of

glacial microbiomes enduring the harsh conditions of extremely low temperature, oligotrophy,

and desiccation.

2024 International Meeting of the Microbiological Society of Korea

222 | www.msk.or.kr

GS4-10

Gain-of-Function Mutation in the Galactitol-specific GatC

Transporter Facilitates Glucose Uptake in E. coli

Su On Jeong1, Hyun Ju Kim2, and Sang Jun Lee1*

1Department of Systems Biotechnology, and Institute of Microbiomics, Chung-Ang University

2Biological Resources Research Department, Nakdonggang National Institute of Biological Resources

Glucose is the preferred carbon and energy source for many bacteria, efficiently transported

into the cytoplasm via the phosphotransferase system (PTS). In the absence of glucose-specific

transporters encoded by ptsG or manX, other sugar transporters, such as ExuT, can facilitate

glucose uptake in E. coli. In this study, we adapted PTS-deficient ΔptsHI E. coli strains under

anaerobic conditions. Genome sequencing of the adapted cells identified gain-of-function

mutations in gatC, which encodes the galactitol-specific PTS EIIC. We observed that a missense

mutation (F340C) in the gatC gene allowed adapted cells to uptake glucose efficiently.

CRISPR-Cas9-mediated site-specific genome mutagenesis revealed that altering F340 to other

amino acids in GatC affects glucose uptake rates. Furthermore, significant amounts of succinate

were produced when the gatC mutants were grown in the glucose containing fermentation

medium. This study enhances our understanding of specificity in sugar transport systems and

fermentative metabolic flux in microbial cells and highlights the potential for engineering

microorganisms for biotechnological applications.

www.msk.or.kr | 223

Graduate Students’ Forum

GS4-11

Molecular Identification of Cryptococcus Isolated from

Korean Patients and Discovery of Drug Resistance

Mechanism

Eui-Seong Kim1, Chang-Seop Lee2*, and Kyung-Tae Lee1*

1Korea Zoonosis Research Institute, Jeonbuk National University

2Department of Internal Medicine, Jeonbuk National University Medical School

Cryptococcus neoformans is a pathogenic fungus that causes more than 1.9 million cases and

1.47 million deaths worldwide annually. In this study, strains 5E9 and 5F1 were isolated from

the cerebrospinal fluid of two Korean patients and cultured as single strains for molecular

diagnosis. Sequencing of the internal transcribed spacer 1 (ITS1) revealed that the two strains

were 100% identical to the C. neoformans type strain H99. The genomic region of STE20a,

which indicates the mating type, identified strain 5E9 as C. neoformans MATα. Both clinical

strains showed drug resistance to azole drugs and shared the same mutation in the ERG11

gene. However, interestingly, only strain 5E9 exhibited extremely high resistance to azoles.

Strain 5F1 significantly induced genes related to ergosterol biosynthesis in response to

fluconazole treatment and showed a different pattern of ploidy under drug treatment

conditions. Notably, neither strain responded to fluconazole in the in vivo infection model.

Identification and analysis of antifungal resistance in patient-derived isolates can enable more

rapid diagnosis and treatment of drug-resistant infections and elucidate the mechanisms by

which clinical isolates develop drug resistance.

Poster

A. Systematics / 미생물분류

B. Ecology and Environmental Microbiology / 생태･환경미생물

C. Applied Microbiology / 응용미생물

D. Immunology and Microbial Pathogenesis / 면역･병원미생물

E. Physiology and Biochemistry / 생리･생화학

F. Genetics / 미생물유전학

G. Biotechnology / 생물공학

H. Others / 기타

2024 International Meeting of the Microbiological Society of Korea

226 | www.msk.or.kr

A001

Aurantiacibacter flavus sp. nov. and Aurantiacibacter

gilvus sp. nov., Isolated from the Mudflat of Suaeda

japonica Makino Colonies

Sunho Park, Inhyup Kim, Haejin Woo, Hyunji Lee, Subin Yook, and

Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A002

Halomonas pelophila sp. nov., Isolated from Mud and

Halomonas aquatica sp. nov., Isolated from Marine

Water

Subin Yook, Inhyup Kim, Sunho Park, Haejin Woo, Hyunji Lee, and

Taegun Seo*

Department of Life science, Dongguk University-Seoul

A003

Flavobacterium arundinis sp. nov., Flavobacterium

calami sp. nov., Flavobacterium helocola sp. nov., and

Flavobacterium flavipallidum sp. nov., Isolated from

Stagnant Water in a Clump of Phragmites japonica

Steud

Sunho Park, Inhyup Kim, Haejin Lee, Hyunji Lee, Subin Yook, and

Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A004

Draft Genome Sequence Analyses of Two Novel

Marinobacter suadae sp. nov. and Wenyingzhuangia

gilva sp. nov. Isolated from the Root of Suaeda japonica

Makino

Sunho Park, Inhyup Kim, Haejin Woo, Hyunji Lee, Subin Yook, and

Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A005

Sphingomonas rustica sp. nov. and Sphingomonas

agrestis sp. nov., Novel Carotenoid-producing Bacterial

Strains Isolated from Farm Soil

Hyunji Lee, Inhyup Kim, Sunho Park, Haejin Woo, Subin Yook, and

Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A006

Three Novel Bacterial Species, Roseateles gilvus sp

nov., Roseateles paludis sp. nov., and Roseateles

hydrophilus sp. nov., Isolated from Various

Water-related Environments

Haejin Woo, Geeta Chhetri, Inhyup Kim, Sunho Park, Hyunji Lee,

Subin Yook, and Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A007

Two Novel Bacterial Species, Rhodanobacter

lycopersici sp. nov. and Rhodanobacter geophilus sp.

nov., with Indole-3-acetic Acid Production Ability

Isolated from the Rhizosphere of Solanum

lycopersicum

Haejin Woo, Geeta Chhetri, Inhyup Kim, Sunho Park, Hyunji Lee,

Subin Yook, and Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A008

Roseateles subflavus sp. nov. and Roseateles aquae sp.

nov., Isolated from Artificial Pond Water and

Roseateles violae sp. nov., Isolated from a Viola

mandshurica Root

Haejin Woo, Geeta Chhetri, Inhyup Kim, Yoonseop So, Sunho Park,

Yonghee Jung, and Taegun Seo*

Department of Life Science, Dongguk University-Seoul

A009

Genome-based Reclassification of the Genus Niabella

and the Description of Paraniabella aurantiaca gen.

nov., sp. nov., Isolated from Soil

Yong-Seok Kim1,2 and Chang-Jun Cha1*

1Department of Systems Biotechnology and Center for Antibiotic Resistome,

Chung-Ang University, 2DNA Link, Inc.

A010

Segatella intestisanus sp. nov., a Novel Probiotic with

Antioxidant, Anti-inflammatory, and Antidiabetic

Properties, Isolated from Human Faeces

Md Shamsuzzaman1,2, Ram Hari Dahal1,2, Shukho Kim1,2,

Yoon-Jung Choi1,2, and Jungmin Kim1,2*

1Department of Biomedical Science, School of Medicine, Kyungpook National

University, 2Department of Microbiology, School of Medicine, Kyungpook

National University

A011

Undibacterium cyanobacteriorum sp. nov., an

Azuxin-producing Bacterium Isolated from Fresh Water

during Cyanobacterial Bloom Period

So-Ra Ko1, Ve Van Le1, Loan Thi Thanh Nguyen2, Jin-Cheol Kim2,

Yuna Shin3, Kyunghyun Kim3, and Chi-Yong Ahn1*

1Korea Research Institute of Bioscience & Biotechnology, 2Chonnam National

University, 3National Institute of Environmental Research

A012

The Detection and Isolation of Anaplasma capra from

Korean Cattle

Young-Uk Chung and Jun-Gu Kang*

Korea Zoonosis Research Institute, Jeonbuk National University

Poster

www.msk.or.kr | 227

A013

Acidaminococcus faecalis sp. nov., an Indole-producing

Bacterium Isolated from Feces of an Elderly Woman

Byung Chun Jang1, Chan-Hyeok Park1, Haneol Yang1, Seunghyun

Kim1, Do-Yun Kim1, and Doo-Sang Park1,2*

1Korean Collection for Type Cultures (KCTC), Korea Research Institute of

Bioscience and Biotechnology (KRIBB), 2KRIBB School of Bioscience, Korea

University of Science and Technology

A014

Two Novel Marine Bacteria in the Reichenbachiellaceae

Isolated from Sanderia malayensis, and Proposal of

Partial Reclassification of the Cytophagales

Yeong-Jun Park1,2, Sung-Hyun Yang1, Seungshic Yum2,3, Nayoung

Lee3, and Kae Kyoung Kwon1,2*

1Korea Institute of Ocean Science and Technology, 2KIOST school, University

of Science and Technology, 3Ecological Risk Research Division, Korea Institute

of Ocean Science and Technology

A015

Thalassobella sediminis sp. nov. and Thalassobella

citrea sp. nov., Isolated from Tidal Flat Sediment

Sung-Hyun Yang1, Mi-Jeong Park1, Yeong-Jun Park1,2, and Kae

Kyoung Kwon1,2*

1Marine Biotechnology Research Center, Korea Institute of Ocean Science &

Technology, 2KIOST School, University of Science and Technology

A016

Metabolic Regulation via the Nitrogen Phosphotransferase

System in Rhodopseudomonas palustris

SeungHee Choi and Yeong-Jae Seok*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

A017

Comparative Genome Analysis of Sphingobacterium

oryzagri sp. nov., and Closely Related Species

Seunghwan Kim1, Hyorim Choi1,2, Yunhee Choi1,3, Jun Heo1, Daseul

Lee1, Byeong-Hak Han1, Seung-Beom Hong1, and Yiseul Kim1*

1Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration, 2Division of Life sciences, Jeonbuk

National University, 3Division of Biotechnology, Jeonbuk National University

A018

Comparative Genome Analysis of Lacticaseibacillus

pabuli sp. nov. and Closely Related Species

Seunghwan Kim1, Hyorim Choi1,2, Yunhee Choi1,3, Yiseul Kim1,

Daseul Lee1, Byeong-Hak Han1, Seung-Beom Hong1, Sangbum

Kim1, and Jun Heo1*

1Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration, 2Division of Life sciences, Jeonbuk

National University, 3Division of Biotechnology, Jeonbuk National University

A019

Isolation and Characterization of Three Novel

Streptococcus Species from the Human Oral Cavity:

Streptococcus dentalis sp. nov., Streptococcus

gingivalis sp. nov., and Streptococcus lingualis sp. nov.

Beom Jin Goo and Jin-Woo Bae*

Department of Biology, College of Science, Kyung Hee University

A020

Genotypic and Phenotypic Characterization of

Cutibacterium acnes Isolates from Acnes Vulgaris

Patients and Healthy Controls

Joo Hun Shin1,2, Rana Md Shohel1,2, Weon Ju Lee3, and Sukho

Kim1,2*

1Department of Biomedical Science, School of Medicine, Kyungpook National

University, 2Department of Microbiology, School of Medicine, Kyungpook

National University, 3Department of Dermatology, Kyungpook National

University School of Medicine

A022

Description and Whole Genome of Oryzobacter telluris

sp. nov., Isolated from Bank-Side Soil in Osucheon

Stream, Republic of Korea

Sumin Jang, Jaeduk Goh, and Ahyoung Choi*

Nakdonggang National Institute of Biological Resources

A023

A Report of 43 Unrecorded Bacterial Species Belonging

to the Phyla Pseudomonadota Isolated from the

Freshwater Environmental in Republic of Korea

Soo-Yeong Lee*, Jaeduk Goh, Ahyoung Choi, Hyangmi Kim, Ji

Young Jung, Ja Young Cho, and Hyun Ju Kim

Biological Resources Research Department, Nakdonggang National Institute

of Biological Resources (NNIBR)

A024

Isolation and Characterization of a Novel Emticicia

Strain Isolated from a Freshwater Reservoir

Hyangmi Kim, Ahyoung Choi, Ji Young Jung, Soo-Yeong Lee, Hyun

Ju Kim, Hye Kyeong Kang, Sumin Jang, Yujin Hwang, and Jaeduk

Goh*

Nakdonggang National Institute of Biological Resources

A025

Sphingomonas resistens sp. nov. and Sphingomonas

degradans sp. nov., Isolated from the Han River

Seung-Tae Kim, Miryung Kim, and Chang-Jun Cha*

Department of Systems Biotechnology and Center for Antibiotic Resistome,

Chung-Ang University

2024 International Meeting of the Microbiological Society of Korea

228 | www.msk.or.kr

A026

Halomonas halophytica sp. nov. and Halomonas salinisoli

sp. nov., Two Novel species Isolated from Coastal Soil

Hyomin Seo1, Seung Yeol Shin1,2, Yihyun Jeon1, Haneul Kim3,

Jaeho Song1, Eunji Kong4, Yochan Joung4, Kiseong Joh5, and

Heeyoung Kang1*

1Division of Microbiology, Honam National Institute of Biological Resources,

2Department of Microbial Biotechnology of Science & Technology, Mokwon

University, 3Advanced Research Center for Island Wildlife Biomaterials, Honam

National Institute of Biological Resources, 4Department of Biotechnology

and Chemical Engineering, Gyeonggi University of Science and Technology,

5Department of Bioscience and Biotechnology, Hankuk University of Foreign

Studies

A027

Aestuariibius marinus sp. nov., Isolated from Surface

Seawater

Hyomin Seo1, Seung Yeol Shin1,2, Yihyun Jeon1, Heeyoung Kang1,

and Jaeho Song1*

1Division of Microbiology, Honam National Institute of Biological Resources,

2Department of Microbial Biotechnology of Science & Technology, Mokwon

University

A028

Pelagovum maritimum sp. nov., Isolated from Surface

Seawater

Seung Yeol Shin1,2, Hyomin Seo1, Yihyun Jeon1, Heeyoung Kang1,

and Jaeho Song1*

1Division of Microbiology, Honam National Institute of Biological Resources,

2Department of Microbial Biotechnology of Science & Technology, Mokwon

University

A029

Diversity of Cladosporium (Cladosporiales,

Cladosporiaceae) Species in Marine Environments and

Report on Five New Species

Wonjun Lee, Ji Seon Kim, Chang Wan Seo, Jun Won Lee, Sung

Hyun Kim, Yoonhee Cho, and Young Woon Lim*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

A030

Chitinibacter sediminis sp. nov., Isolated from the

Sediment of Freshwater Pond

Ji Young Jung, Hye-Kyeong Kang, and Jae Duk Goh*

Biological Resources Research Department, Nakdonggang National Institute

of Biological Resources

A031

Integrating Bioinformatics Tools to Characterize the

Functional Gut Microbiome Diversity in Healthy

Koreans

Joy Ekpenyong, Fathrinah Binti Kohadie, and Robert J. Mitchell*

Department of Biological Science, Ulsan National Institute of Science and

Technology

A032

Description of Luteibacter aegosomatissinici sp. nov.

Luteibacter aegosomatis sp. nov., and Luteibacter

aegosomaticola sp. nov. Isolated form the Gut of

Aegosoma sinicum sinicum Larvae

Hae In Joe, Jee-Won Choi, and Jin-Woo Bae*

Department of Biology, Department of Biomedical and Pharmaceutical

Sciences, Kyung Hee University

A033

Asticcacaulis carbonis sp. nov., Isolated from a

Sediment in a Coal Mine Drainage Treatment System

Hyekyeong Kang, Jae Duk Goh, and Ji Young Jung*

Nakdonggang National Institute of Biological Resources

A034

Litoriludica sedimotoria gen. nov., sp. nov., a Novel

Bacterium Isolated from Tidal-flat Sediment

Jaincy N Jayan1, Ke Dong2*, Sathiyaraj Srinivasan3*, and

Sang-Seob Lee1*

1Department of Integrative Biotechnology, Sungkyunkwan University, Natural

Science Campus, 2Life Science Major, Kyonggi University, 3Department of

Bio and Environmental Technology, Seoul Women's University

A035

Characterization of Four Novel Yeast Species of

Metschnikowia and Their Potential for Agricultural Uses

Yunhee Choi1,2, Jun Heo1, and Yiseul Kim1*

1Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration, 2Department of Life Sciences, Jeonbuk

National University

A036

First Report of Lelliottia amnigena Causing Soft Rot on

Heracleum moellendorffii in South Korea

A Yeong Heo, Jiwon Kim, Ki Hyeong Park, Sang-Tae Seo, and

Chung Ryul Jung*

Forest Entomology and Pathology Division, National Institute of Forest

Science

A037

Roseobacter algicola sp. nov. and Sulfitobacter

rhodophyticola sp. nov., Isolated from Marine Red Algae

Seo Hui Choi, Jeong Min Kim, Byeong Jun Choi, and Che Ok Jeon*

Department of Life Science, Chung-Ang University

A038

First Report of Agaricus sinoplacomyces (Agaricomycetes,

Agaricaceae) in Korea

Yihyun Jeon, Ji Hye Jeong, Joo Hyun Jin, Jong Won Jo, and Hae Jin

Cho*

Honam National Institute of Biological Resources

Poster

www.msk.or.kr | 229

A039

Nine Newly Recorded Species of Amanita (Agaricomycetes,

Amanitaceae) in Island Regions of Korea

Jihye Jeong1, Yihyun Jeon1, Joo Hyun Jin1, Jae Young Park2, Nam

Kyu Kim3, Hyun-Ju Noh1, Jong Won Jo1, and Hae Jin Cho1*

1Honam National Institute of Biological Resources, 2Q-myco Co, 3Department

of Forest Healing, College of Human service, Catholic Kwandong University

A040

Nocardioides eburnea sp. nov., a Potential

Catechol-degrading Bacterium Isolated from

Commercial Bio-fertilizer Soil

Minseok Kang1, Jae-yon Yu2, Ke Dong3, Sathiyaraj Srinivasan4*,

and Sang-Seob Lee1*

1Department of Integrative Biotechnology, College of Biotechnology and

Bioengineering, Sungkyunkwan University, 2Korean Environmental Microorganism

Resources Center, Institute of Biotechnology and Bioengineering, Sungkyunkwan

University, 3Department of Biological Sciences, Kyonggi University, 4Department

of Bio and Environmental Technology, Seoul Women's University

A041

Demequina capsici sp. nov., a Novel Plant

Growth-promoting Actinomycete Isolated from the

Rhizosphere of Bell Pepper (Capsicum annuum)

Zalfa Humaira1,2, Donghyun Cho1, Yuxin Peng1, Yulim Park1, and

Jiyoung Lee1*

1Korean Collection for Type Cultures (KCTC), Biological Resource Center,

Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Department

of Biosystems and Bioengineering, KRIBB School of Biotechnology, University

of Science and Technology (UST)

A042

Mucilaginibacter jeongeupensis sp. nov., a Novel

Endophytic Bacteria Isolated from Apple Tree Leaves

Yu Lim Park1,2, Yuxin Peng1, Donghyun Cho1, Zalfa Humaira1, and

Jiyoung Lee1*

1Korean Collection for Type Cultures (KCTC), Biological Resource Center,

Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Biosystems

and Bioengineering, KRIBB School of Biotechnology, Korea National University

of Science and Technology (UST)

A043

Isolation of Three Novel Species Candidates in the

Streptococcus Genus from Human Saliva

Young Sik Choi and Jin-Woo Bae*

Department of Biology, College of Science, Kyung Hee University

A044

Development and Application of the Novel Marker for

Identification of Gluconobacter Species

Jun Heo, Hyorim Choi, Seunghwan Kim, and Yiseul Kim*

Agricultural Microbiology Division, National Institute of Agricultural Sciences

A045

Antagonistic Potential of Streptomyces spp. from Soil

against Antibiotic Resistance Bacteria

DaChan Kim1, Jino Son2, and Seung Bum Kim1

1Department of Microbiology and Molecular Biology, College of Bioscience

and Biotechnology, Chungnam National University, 2Biological Resources

Assessment Division, National Institute of Biological Resources

A046

Isolation and Characterization of Antimicrobial

Compounds from Micromonospora spp. Inhabiting

Riverside Soil

Chaerin Jeong and Seung Bum Kim*

Department of Microbiology and Molecular Biology, Chungnam National

University

A047

Taxonomic Analysis of Chryseobacterium daejeonensis

sp. nov. Isolated from Soil

DaChan Kim, ChaeRin Jeong, JinHo Kim, and Seung Bum Kim*

Department of Microbiology and Molecular Biology, College of Bioscience

and Biotechnology, Chungnam National University

A048

Thalassotalea aquiviva sp. nov., and Thalassotalea

maritima sp. nov., Isolated from Seawater of the Coast

in South Korea

Jina Lee1, Seung-Hui Song1, Kira Moon1, Nakyeong Lee1, Sangdon

Ryu1, Hye Seon Song1, Sung Moon Lee1, Yun Ji Kim1, Se won

Chun1, Kyung-Min Choi2, and Aslan Hwanhwi Lee1*

1Division of Environmental Materials, Honam National Institute of Biological

Resources (HNIBR), 2Department of Integrative Bioresources, Honam National

Institute of Biological Resources (HNIBR)

A049

Metabacillus marisymbiosis sp. nov., a Novel Species

Isolated from Seashore Sediment

Youngmi Lee1, Jaincy N Jayan1, Ha Jung Moon2, Ke Dong3,

SathiyaRaj Srinivasan4*, and Sang-Seob Lee2*

1Integrative Biotechnology, Sungkyunkwan University, 2Integrative Biotechnology,

Sungkyunkwan University, 3Life Science Major, Kyonggi University, 4Department

of Bio and Environmental Technology, Seoul Women's University

A050

Proposal of 18 Novel Genera in the Family

Colwelliaceae by a Genome-based Reclassification and

Newly Isolated with Three Genera, One Species

Min Seo Lee1,2, Kae Kyoung Kwon1,3*, and Jin-Sook Park2*

1Marine Biotechnology Research Center, Korea Institute of Ocean Science &

Technology, 2Department of Biological Sciences and Biotechnology,

Hannam University, 3KIOST School, University of Science and Technology

2024 International Meeting of the Microbiological Society of Korea

230 | www.msk.or.kr

A051

Ruegeria discoderminis sp. nov., Ruegeria jejuensis sp. nov.,

and Ruegeria hyattica sp. nov., Isolated from Marine Sponges

Yea Lin Moon, Ji-Yoon Shin, and Jin-Sook Park*

Department of Biological Sciences and Biotechnology, Hannam University

A052

Identification of Two Novel Bacterial Species of the

Genus Lacibacter: Lacibacter aquaticus sp. nov. and

Lacibacter koreense sp. nov., Isolated from Fresh Water

Kyu Hyun Lee, Jung-Hun Joo, and Wan-Taek Im*

Hankyong National University

A053

Aureibaculum koreanum sp. nov., and Aureibaculum

conchae sp. nov., Isolated from the Marine Mollusks

Soo Bin Kim and Jin-Sook Park*

Department of Biological Sciences and Biotechnology, Hannam University

A054

Characterization of Aspergillus oryzae Isolated from

Korean Traditional Soybean Meju

He-Jin Cho1, Ye-Jin Kang2, Kyu-Hyun Kim1, and Hee-Soo Park1,2*

1School of Food Science and Biotechnology, Kyungpook National University,

2Department of Integrative Biology, Kyungpook National University

A055

Microbacterium xanthum sp. nov., Isolated from the

Han River, South Korea

Gracia Pradnya Lolita and Chang-Jun Cha*

Department of Systems Biotechnology, Chung-Ang University

A056

Agromyces jejuensis sp. nov. and Agromyces amylolyticus

sp. nov., Two New Species Isolated from Jeju Soil

Jiwon Seo, Chaerin Jeong, Jin-Il Kim, Jin-Ho Kim, and Seung Bum

Kim*

Department of Microbiology and Molecular Biology, College of Bioscience

and Biotechnology, Chungnam National University

A057

A Novel Thermophilic Polyhydroxyalkaonate (PHA)

Producing Bacterium, Thermohahella caldifontis gen.

nov. sp. nov., Isolated from Hot Spring

Sondor Ganbat1, Dariimaa Ganbat1, Jae-Yoon Sung2, Yujin Son3, Ji

Yeong Park1, Joo Young Yang1, Seong-Bo Kim4, Dong-Woo Lee2,

Mi-Hwa Park5, and Sang-Jae Lee1*

1Department of Food Biotechnology and Research Center for Extremophiles

and Marine Microbiology, Silla University, 2Department of Biotechnology,

Yonsei University, 3Department of Integrative Biotechnology (IBT), Yonsei

University, 4Bio-Living Engineering Major, Global Leaders College, Yonsei

University, 5Department of Food and Nutrition, Silla University

A058

Isolation and Characterization of Bacteriophages

Infecting Nanopelagicales (the acI Lineage), a Bacterial

Group Prevalent in Freshwater Environments

Minjeong Kim1, Suhyun Kim2, Ilnam Kang1, and Jang-Cheon Cho1*

1Department of Biological Sciences and Bioengineering, Inha University,

2Center for Molecular and Cell Biology, Inha University

A059

A Bacterial Novel Genus Croceiramulus Isolated from a

Tidal Flat of Yongyu-do in South Korea

Yeonjae Yoo, Sang Hyun Lee, Dae Young Kwon, Sunlul Kwon,

Minseo Cho, and Jae-jin Kim*

Division of Environmental Science & Ecological Engineering, College of Life

Sciences & Biotechnology, Korea University

A060

Eighteen New Records of Macrofungi in Mudeungsan

National Park

Minseo Cho1, Sun Lul Kwon1,2, Yeonjae Yoo1, Sang Hyun Lee1, Dae

Young Kwon1, Changmu Kim3, and Jae-Jin Kim1*

1Division of Environmental Science and Ecological Engineering, College of

Life Sciences and Biotechnology, Korea University, 2BK21 FOUR R&E Center

for Environmental Science and Ecological Engineering, Korea University,

3Species Diversity Research Division, National Institute of Biological Resources

A061

Taxonomic Characterization and Evaluation of Antimicrobial

Potential of Streptomyces spp. Isolated from Soil

Jin-Il Kim1, Da-Chan Kim1, Jin-Ho Kim1, Ji-Won Seo1, Jino Son2, and

Seung Bum Kim1*

1Department of Microbiology and Molecular Biology, College of Bioscience

and Biotechnology, Chungnam National University, 2Biological Resources

Assessment Division, National Institute of Biological Resources

A062

Mariniflexile halophytorum sp. nov. and Mariniflexile

salinisoli sp. nov., Two Novel Species Isolated from

Coastal Soil

Heeyoung Kang1, Seung Yeol Shin1,2, Yihyun Jeon1, Hyomin Seo1,

Jaeho Song1, Seohyeon Bae3, Yochan Joung3, Haneul Kim4, and

Kiseong Joh5*

1Division of Microbiology, Honam National Institute of Biological Resources,

2Department of Microbial Biotechnology of Science & Technology, Mokwon

University, 3Department of Biotechnology and Chemical Engineering, Gyeonggi

University of Science and Technology, 4Advanced Research Center for

Island Wildlife Biomaterials, Honam National Institute of Biological Resources,

5Department of Bioscience and Biotechnology, Hankuk University of Foreign

Studies

A063

In Silico Chemotaxonomy of the Genus Amycolatopsis

Bilguun Oyuntsetseg and Seung Bum Kim*

Chungnam National University

Poster

www.msk.or.kr | 231

A064

Polaribacter rhizosphericum sp. nov Strain 2HN02-6, A

New Bacterial Species Isolated from Rhizosphere Soil of

Phragmites communis

Yuna Lee, Da Som Kim, Ho-Young Shin, and Song-Ih Han*

Department of Microbial Biotechnology, Mokwon University

A065

Analysis of Microflora in Ngapi, Myanmar Traditional

Sauce

Ji Yun Hwang1, Ah-Ran Lee2, and Soo-Ki Kim1*

1Department of Animal Science, Konkuk University, 2Animal Resources Research

Center, Konkuk University

A066

Probiotic Characterization of Fermented Mugwort and

Water Spinach

Hung Trinh The1, Jae-Hyeong Shin1, Ju-Hyun Song1, Jeong-Hyun

Park1, Ah-Ran Lee2, and Soo-Ki Kim1*

1Department of Animal Science, Konkuk University, 2Animal Resources Research

Center, Konkuk University

A067

Genomic Mining of Novel Lanthipeptide Producer:

Ferruginibacter sp.

Chunzhi Jin1, Ye Zhuo1,2, and Hyung-Gwan Lee1,2*

1Cell Factory Research Centre, Korea Research Institute of Bioscience &

Biotechnology (KRIBB), 2Department of Environmental biotechnology,

KRIBB School of Biotechnology, Korea University of Science and Technology

(UST)

A068

Genomic Analysis of Photosynthesis in Two Novel

Freshwater Origin Sphingomonas sp.

Chunzhi Jin1, Ye Zhuo1,2, Long Jin3, and Hyung-Gwan Lee1,2*

1Cell Factory Research Centre, Korea Research Institute of Bioscience &

Biotechnology (KRIBB), 2Department of Environmental biotechnology, KRIBB

School of Biotechnology, Korea University of Science and Technology

(UST), 3Co-Innovation Centre for Sustainable Forestry in Southern China,

College of Ecology and the Environment, Nanjing Forestry University,

Nanjing 210037, China

A069

Taxonomic Study of a Novel Species of the Family

Lachnospiraceae Isolated from Healthy Korean

Min Kuk Suh1,2, Ji-Sun Kim1, Mi Kyung Eom1, and Jung-Sook Lee1,3*

1Korean Collection for Type Cultures, Korea Research Institute of Bioscience

and Biotechnology, 2Department of Lifestyle Medicine, Jeonbuk National

University, 3University of Science and Technology (UST)

2024 International Meeting of the Microbiological Society of Korea

232 | www.msk.or.kr

B001

Characteristics of Fecal Coliforms in the Sand of

Children's Playground

Hyun-kyung Lee, Ji-Young Kyung, Gyun-Hee Kim, Ji-Soo Kim,

Yun-Ha Jung, Ju-Hee Im, and Dong-Wan Kim*

Gyeonggi-do Institute of Health and Environment

B002

2024 Infectious Disease Monitoring in the

wastewater-Based Infectious Disease Surveillance

System in Chungnam-do

Insook Cho, Yeonji Gim, Hyeonjeung Kim, and Myounghee Shin*

Chungcheongnam-do Institute of Health & Environment Research

B003

Effects of Fungal Spore Size on Recovery from

Membrane Filters for Air Filtration

So-Yeon Jeong, Chi Won Lee, and Tae Gwan Kim*

Pusan National University

B004

Comparison of MALDI-TOF MS and 16S rRNA

Sequencing for the Identification of Legionella Species

Isolated from Artificial Water Systems

Hee-Young Hwang and Mee-Jeoung Kim*

Gyeongsangbuk-do Government Public Institute of Health & Environment

B005

Marine Bacterial Communities and Bacillus Species for

Probiotics in Sea Cucumber Farms

Seyeon Shin1,2 and Hyung-Yeel Kahng1,3*

1Department of Environmental Education, Sunchon National University,

2Haenam-gun Agricultural Technology Center, 3SCNU Research and Education

Center for Climate Change

B006

Can Lichens Truly Reflect Environmental Pollution

Levels?

Jiho Yang1, Sangwoo Ji2, Dong-Wan Cho2, Pyeong-Koo Lee2, and

Jae-Seoun Hur3*

1Gyeongnam Bio and Anti-aging Core Facility, 2Korea Institute of Geoscience

and Mineral Resources, 3Sunchon National University

B007

Host Genus Trumps Substrate in Shaping Lichen

Microbiome in Regional Study

Jiho Yang1 and Jae-Seoun Hur2*

1Gyeongnam Bio and Anti-aging Core Facility, 2Sunchon National University

B008

Characterization of Microbial Communities in Different

Types of Cow Manure

Hokyung Song1, Geon Choi2, and Tatsuya Unno2*

1Department of Environmental Engineering, Chosun University, 2Department

of Microbiology, Chungbuk National University

B009

Specific Spatial Dynamics in Staphylococcus aureus

Interactions with Malassezia Lead to Enhanced

Pathogenicity

Eun Sun Lyou and Tae Kwon Lee*

Department of Environmental & Energy Engineering, Yonsei University

B010

Vaginal Microbiome Composition in Korean Women and

Its Association with Preterm Birth

Minjeong Kim1, Inseon Kim1, Mihee Oh1, Jangheon Lee1, Seeun

Lim1, JeeYoon Park2,3, and Sunmi Yoon1*

1Macrogen Inc., 2Department of Obstetrics and Gynecology, Seoul National

University College of Medicine, 3Department of Obstetrics and Gynecology,

Seoul National University Bundang Hospital

B011

Characterization of a Novel Cold-active Pseudomonas

Strain Causing Potato Soft-rot Disease from Greenland

Tundra Soil

Sungho Woo1,2, Mincheol Kim1, Yung Mi Lee1, and Dockyu Kim1*

1Division of Life Sciences, Korea Polar Research Institute, 2Department of

Plant Biotechnology, Korea University

B012

Comparative Evaluation of the Rumen Microbiota of

Hanwoo, Jeju Black cattle, and Their Crossbreds

Sungju Jung1, Cheolju Park1, Minseok Kim1, Seunghyeon Mun1,

Jaeyong Song2, Joong Kook Park2, Yong Kwan Kim3, and Min Seok

Kim1*

1Chonnam National University, 2Nonghyup Feed Co., LTD., 3Seogwiposi

Chuckhyup

B013

Rhizosphere Microbiome Diversity in a Vegetation Hot

Spot of Northernmost Greenland

YoHan So1,2, Nu Ri Myeong1, Taeyoon Park1, Ji young Jung1,

Soojung Jung1, and Mincheol Kim1*

1Korea Polar Research Institute, Incheon 21990, Republic of Korea, 2Department

of Systems Biology, Yonsei University

Poster

www.msk.or.kr | 233

B014

Isolation and Genomic Characterization of the OPB56

Clade Bacteria from Freshwater Environments

Sumin Kim, Suhyun Kim, Ilnam Kang, and Jang-Cheon Cho*

Department of Biological Sciences and Bioengineering, Inha University

B015

Microbial Community Composition and acI Lineage

Distribution Across Diverse Freshwater Ecosystems in

South Korea

Hyunwook Sa, Suhyun Kim, Ilnam Kang, and Jang-Cheon Cho*

Department of Biological Sciences and Bioengineering, Inha University

B016

NAD Dependency in Freshwater acI Bacteria Identified

through Genomic and Cultivation Approaches

Jisoo Han, Suhyun Kim, Ilnam Kang, and Jang-Cheon Cho*

Department of Biological Sciences and Bioengineering, Inha University

B017

Metagenomic Analysis for the Ecological Monitoring of

Bacterial Diversity from Four Rivers

Bora Jang and In-Tae Cha*

National Institute of Biological Resources

B018

Microbial Diversity Comparison by Metagenomic

Analysis from Dokdo and East Sea Coast

Bora Jang and In-Tae Cha*

National Institute of Biological Resources

B019

Microbial Connectivity and Adaptive Strategies Driving

Biogeochemical Cycling in Ice-covered Lakes of the

McMurdo Dry Valleys, Antarctica

Hanbyul Lee1, John Priscu2, and Ok-Sun Kim1*

1Division of Life Sciences, Korea Polar Research Institute, 2Department of

Land Resources and Environmental Sciences, Montana State University,

USA

B020

Biodegradation of Synthetic Plastic High-Density

Polyethylene (HDPE) by Microorganisms Isolated from

the Activated Sludge

Yedam Kim, Gyulim Park, Young Seok Lee, Youngsuk Kim, and

Hong-Joo Son*

Department of Life Science and Environmental Biochemistry, Pusan National

University

B021

Characteristic of Scalp Microbiome According to the

Severity of Alopecia

Jin-Jae Lee1, Dong-Woo Lee2, Gaewon Nam3, and Bong-Soo Kim1*

1Department of Life Science and Multidisciplinary Genome Institute, Hallym

University, 2Department of Biotechnology, Yonsei University, 3Department

of Cosmetic Science and Technology, Seowon University

B022

The Infecting Viral Genes Captured from Bulk-metagenomic

Data in Ice-covered Lake Bonney, Antarctica

Yeo-Eun Yun1, Hanbyul Lee1, John Priscu2, and Ok-Sun Kim1*

1Division of Life Sciences, Korea Polar Research Institute, 2Department of

Land Resources and Environmental Sciences, Montana State University, USA

B023

Identification of Indicator Gene for Tracking Antibiotic

Resistance Gene Dissemination in Greenhouses, South

Korea

Raan Shin, Jaeyoung Ro, Sujin Lee, and Hor-Gil Hur*

Gwangju Institute of Science and Technology

B024

Depth-specific Bacterial Diversity in the Styx Glacier

with Potential Pathogens

Minkyung Kim1, Hanbyul Lee1, Subin Lee1, Yeongcheol Han2, and

Ok-Sun Kim1*

1Division of Life Sciences, Korea Polar Research Institute, 2Division of Glacial

Environment Research, Korea Polar Research Institute

B025

Muropeptide Recycling Permease, AmpG, Functions as

a Morphological Determinant in Acinetobacter

baumannii

Taeyeong Kim and Woojun Park*

Korea University

B026

Exploring Plankton Communities with eDNA

Metabarcoding in the Amundsen Sea, Southern Ocean

Dukki Han

Gangneung-Wonju National University

B027

Elevational Variation of Soil Fungal Communities in

Mountain Ecosystems of the Korean Peninsula

Lei Chen1, Guangyun Zhen2, Xueqin Lu2, Ke Dong3, and Sang-Seob

Lee1*

1Department of Integrative Biotechnology, Sungkyunkwan University,

2School of Ecological and Environmental Sciences, East China Normal University,

Shanghai, P.R. China, 3Life Science Major, Kyonggi University

2024 International Meeting of the Microbiological Society of Korea

234 | www.msk.or.kr

B028

Bamboo Biochar Amendment Enhances Microbial

Community Succession and Boosts Metabolite Yield

During Swine Manure Composting

Mengchen Zhao1, Xueqin Lu2, Dorsaf Kerfahi3, Ke Dong3, and

Sang-Seob Lee1*

1Department of Integrative Biotechnology, Sungkyunkwan University,

2School of Ecological and Environmental Sciences, East China Normal University,

Shanghai, P.R. China, 3Department of Biological Sciences, Keimyung University

B029

The Influence of Ocular Microbiome Interactions during

the Treatment of Dry Eye

Joon-Young Park1, Jin-Jae Lee1, Chang Ki Yoon2, Young Joo Shin3,

and Bong-Soo Kim1,4*

1Department of Life Science, Multidisciplinary Genome Institute, Hallym

University, 2Department of Ophthalmology, Seoul National University College

of Medicine, 3Department of Ohthalmology, Hallym University College of

Medicine, 4The Korean Institute of Nutrition, Hallym University

B030

Korean Gut Microbiome Bank (KGMB)

Jung-Sook Lee

Korean Collection for Type Cultures, Korea Research Institute of Bioscience

and Biotechnology, University of Science and Technology

B031

The Fungal and Bacterial Diversity on the Surfaces of

Building Elevators in a University Campus

Ye-Eun Lee, Su-A In, Ji-Woo Park, and Min-Soo Kim*

Department of Microbiology and Molecular Biology, Chungnam National

University

B032

Fresh Vegetable Virome is Diverse, Abundant, and

Transmitted to the Human Gut Virome

Ji-Woo Park1, Yeo-Eun Yun1, Jin Ah Cho2, Su-In Yoon2, Su-A In1,

Eun-Jin Park3, and Min-Soo Kim1*

1Department of Microbiology and Molecular Biology, Chungnam National

University, 2Department of Food and Nutrition, Chungnam National University,

3Department of Food Bioengineering, Jeju National University

B033

The Seasonal Fungal and Bacterial Microbiota of

Lettuce are Differently Associated with the Human Gut

Microbiome

Su-A In and Min-Soo Kim*

Department of Microbiology and Molecular Biology, Chungnam National

University

B034

Aerobic DNRA-performing Neobacillus iksanensis sp.

nov. Isolated from Paddy Soil

Seohyun Ahn and Jeonghwan Jang*

Division of Biotechnology, Jeonbuk National University

B035

Nitric Oxide Reduction to Ammonium in the

DNRA-catalyzing Bacterium Citrobacter sp. DNRA3

Sojung Yoon1, Paloma Garridor-Amador2, Boran Kartal2, and

Sukhwan Yoon1*

1Department of Civil and Environmental Engineering, Korea Advanced

Institute of Science and Technology (KAIST), 2Max Planck Institute for

Marine Microbiology, Bremen, Germany

B036

Depth-profiled Prokaryotic Community Structure in

Waters Surrounding Seamounts of the Magellan

Seamount Cluster, West Pacific Ocean

Mirae Kim1, Yeonjung Lim2, Ilnam Kang1, and Jang-Cheon Cho1*

1Department of Biological Sciences and Bioengineering, Inha University,

2Center for Molecular and Cell Biology, Inha University

B037

The Impact of Different Feeding Systems on Rumen

Microbiota of Hanwoo Steers

Minseok Kim, Cheolju Park, Seung hyun Mun, Sungju Jung, and

MinSeok Kim*

Division of Animal Science, Chonnam National University

B038

Isolation of Novel Archaeal Virus Infecting Marine

Ammonia-oxidizing Thaumarchaea

Na-Ri Kim, Eun-Jeong Choi, and Jong-Geol Kim*

Department of Biology, Wonkwang University

B039

Antibiotic Resistance Mediated by Non-enzymatic

Inactivation of Ciprofloxacin in Microbacterium sp.

CJ48

Miryung Kim, Le Tran Tien Chau, and Chang-Jun Cha*

Department of Systems Biotechnology and Center for Antibiotic Resistome,

Chung-Ang University

B040

Inactivation of Tylosin by Novel Esterases Conferring

Resistance in Bacillus cereus CJ54

Yu Jin Kim and Chang-Jun Cha*

Department of Systems Biotechnology and Center for Antibiotic Resistome,

Chung-Ang University

Poster

www.msk.or.kr | 235

B041

Longitudinal Dynamics of River Resistome Driven by

Mobile Resistance Genes

Do-Hoon Lee1, Yong-Seok Kim1, Kihyun Lee1,2, Dae-Wi Kim1,3, and

Chang-Jun Cha1*

1Department of Systems Biotechnology and Center for Antibiotic Resistome,

Chung-Ang University, 2CJ Bioscience, 3Division of Life Sciences, Jeonbuk

National University

B042

Viral Metagenome of the Inland Aquaculture Reveals

Auxiliary Metabolic Genes Augmenting Commensal

Microbiome Growth

Kira Moon, Nakyeong Lee, Sang-don Ryu, Jina Lee, Hyesun Song,

Seung Hui Song, Yun Ji Kim, Sung Moon Lee, Se Won Chun, and

Aslan Hwanwhi Lee*

Honam National Institute of Biological Resources

B043

Evaluation of Bacillus JC-18 as Potential Biocontrol

Agent against Tomato Pathogens

Mi Hee Kim1, Ui-Lim Choi1, Youngkun Shim2, Jaejun Oh2, Ji hye

Jung2, and Gun Woong Lee1*

1Jeonju AgroBio-Materials Institute, Future Agriculture Team, 2Microzyme

Co., Ltd. Research and Development Department

B044

Investigation of Bacterial Community Structure of Ten

Tree Species

Seung Yeol Shin1,2, Hyomin Seo1, Jaeho Song1, and Heeyoung

Kang1*

1Division of Microbiology, Honam National Institute of Biological Resources,

2Department of Microbial Biotechnology of Science & Technology, Mokwon

University

B045

Fungal Diversity of the Plastisphere in U-do, Jeju Island

Sumin Jo, Ji Seon Kim, Wonjun Lee, and Young Woon Lim*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

B046

Clostridium iksanensis sp. nov., the Free-living Nitrogen

Fixing Bacterium, Accumulates Ammonium

Extracellularly

Soyeon Park and Jeonghwan Jang*

Division of Biotechnology, Jeonbuk National University

B047

Gut Microbiome Analysis in Abalone (Haliotisdiscus

hannai) and Horned Tuban (Turbo cornutus) Collected

from the Korea Coast with Sea Urchin Barrens

Jae-won Jo, Joon-Young Park, Jin-Jae Lee, and Bong-Soo Kim*

Department of Life Science, Multidisciplinary Genome Institute, Hallym

University

B048

Research on Brown Algae Adhesion through Symbiotic

Microbial Analysis

Yurim Bae1, Dongjin Kim2, Tae Oh Cho2, and Soon-Kyeong Kwon1*

1Division of Applied Life Science (BK21), Gyeongsang National University,

2Department of Life Science, Chosun University

B049

Multi-transcriptome Analysis Revealing

Flavobacterium-mediated Suppression Mechanisms

against Bacterial Wilt Pathogen

Sujin Lee1, Hyein Park2, Boyoung Lee2, Jaehyo Park2, Ju Yeon

Song2, Jihyun F. Kim2, and Soon-Kyeong Kwon1,2*

1Division of Applied Life Science (Brain Korea 21), Gyeongsang National

University, 2Department of Systems Biology, Division of Life Sciences, and

Institute for Life Science and Biotechnology, Yonsei University

B050

Gut Microbiota Synergize Acupuncture Treatment

Responses in Atopic Dermatitis

Yerin Seo1, Sujin Lee1, Kyuseok Kim2, Hi-Joon Park3, and

Soon-Kyeong Kwon1*

1Division of Applied Life Science (BK21), Gyeongsang National University,

2Department of Ophthalmology, College of Korean Medicine, Kyung Hee

University, 3Department of Meridian Medical Science, College of Korean Medicine,

Kyung Hee University

B051

Conversion of Nitrate Contaminated Water to

Ammonium Fertilizer: By Utilizing Hydrogenotrophic

DNRA Catalyzing 2 Campylobacterales Isolates via

MBBR Operation

Minho Lee, Hokwan Heo, Taeho Yoon, and Sukhwan Yoon*

Department of Civil and Environmental Engineering, Korea Advanced Institute

of Science and Technology (KAIST)

B052

Monitoring of Fungal Tree Pathogens in Urban Green

Spaces

Ki Hyeong Park, A Yeong Heo, Jiwon Kim, and Sang-Tae Seo*

Forest Entomology and Pathology Division, National Institute of Forest

Science

2024 International Meeting of the Microbiological Society of Korea

236 | www.msk.or.kr

B053

Isolation and Genomic Characterization of Methanotrophs

Isolated from Rice Paddies

Si Cheol Kim, Ye Jin Gwak, Hong Gi Wook, and Hyo Jung Lee*

Department of Biological Science, Kunsan National University

B054

Analysis of Changes in the Rhizosphere Soil

Environment of Pine Forests in the Uljin after a Wildfire,

Republic of Korea

Seok Hui Lee1, Su Ho Lee2, Yeong Dae Park2, and Jun Won Kang1*

1Department of Forestry, Kyungpook National University, 2School of Forest

Sciences and Landscape Architecture, Kyungpook National University

B055

The Effects of Bacillus subtilis-Based Probiotics

Product on Finishing Pig Growth Performance and Odor

Mitigation in Farms

Sunjoon Lee, Eun Ryul Oh, Seon-Bong Choe, Jun-Hyeong Seo,

Hyun-Jun Jang, and Yangseon Kim*

Center for Industrialization of Agricultural and Livestock Microorganisms

B056

Screening for Microcystin-degrading Bacteria in

Toxin-producing Terrestrial Cyanobacterial Cultures

Nakyeong Lee, Yun Ji Kim, Sangdon Ryu, Kira Moon, Hye Seon

Song, Jina Lee, Se won Chun, Sung Moon Lee, Seunghui Song, and

Aslan Hwanhwi Lee*

Honam National Institute of Biological Resources

B057

Spatio-temporal Variation in the Bacterial Community

Structure of Two Coastal Wetlands in The Korean Bays

Sangdon Ryu, Se won Chun, Sung Moon Lee, Seunghui Song, Yun

Ji Kim, Nakyeong Lee, Kira Moon, Jina Lee, Hye Seon Song, and

Aslan Hwanhwi Lee*

Honam National Institute of Biological Resources

B058

Genomic and Safety Characteristics of Enterococcus

lactis, Isolated from Meju, a Korean Traditional

Fermented Soybean Brick

Dae Gyu Choi, Dong Min Han, and Che Ok Jeon*

Department of Life Science, Chung-Ang University

B059

Roseateles algicola sp. nov. and Roseateles arenisoli sp.

nov., Isolated from a Freshwater Green Alga and Soil

Si Hyeon Lee, Ju Hye Baek, and Che Ok Jeon*

Department of Life Science, Chung-Ang University

B060

Investigation of Key Microbes Responsible for

Metabolizing Aromatic Amino Acids in the Human Gut

and Their Correlations with Diseases

Jae Kyeong Lee, Ju Hye Baek, Yoonsoo Hahn, and Che Ok Jeon*

Department of Life Science, Chung-Ang University

B061

Exploring Geographic and Topological Diversity in the

Skin Microbiome of Asian Women

Hye Lim Keum1, Taeyune Kim1,2, and Woo Jun Sul1*

1Department of Systems Biotechnology, Chung-Ang University, 2META

R&D, Macrogen, Inc.

B062

Microbial Community Impact of Organic Contamination

in Gomso Bay Tidal Flats

Akila Kathiresan1, Hyung-Seop Kim2, Ke Dong3, Lei Chen1,

Hee-Yeon Park2, and Sang-Seob Lee1*

1Department of Integrative Biotechnology, Sungkyunkwan University, Natural

Science Campus, 2Department of Marine Biology, College of Ocean Science

and Technology, Kunsan National University, 3Department of Biological

Sciences, Kyonggi University

B063

Bio-porous Technology for Ammonia and Hydrogen

Sulfide Remediation in Clam-cultivated Tidal Flats

Akila Kathiresan1, Hyung-Seop Kim2, Hee-Yeon Park2, and

Sang-Seob Lee1*

1Department of Integrative Biotechnology, Sungkyunkwan University, Natural

Science Campus, 2Department of Marine Biology, College of Ocean Science

and Technology, Kunsan National University

B064

Polysaccharide Degradation Profiles of Bacteria

Isolated from Phycosphere of Red Marine Macroalgae

Byeong Jun Choi, Jeong Min Kim, Hülya Bayburt, and Che Ok Jeon*

Chung-Ang university

B065

Comprehensive Approach to Identifying Key Factors in

Potato Common Scab-infected Fields

Gyeryeong Bak, Yang-Min Kim, and Jeong-Tae Lee*

Highland Agriculture Research Institute, National Institute of Crop Science

B066

A Study on Distribution of Mushroom Species in Jejudo

Island through Morphological and Sequence Analysis

Seung Hak Lee, Weon-Jong Yoon, and Ji-Gweon Park*

Biodiversity Research Institute, Clean Bio Business Division, Jeju Technopark

Poster

www.msk.or.kr | 237

B067

Leveraging AI to Unify Fragmented Microbiome Research:

Expanding EzBioCloud with Large Language Models

Jeongsup Moon1, Mauricio Chalita1, Yeong Ouk Kim1,2, Nuga

Baek1, Jongsik Chun1*, and Hyun-Seok Oh1*

1CJ Bioscience Inc, 2Interdisciplinary Program in Bioinformatics, Seoul National

University

B068

Understanding Antibiotic Resistance in a Local Community:

Insights from a 'One Health' Perspective in South Korea

Bo-Yun Choi, Jin Ju Kim, Hyeon Jin Kim, and Woo Jun Sul*

Department of Systems Biotechnology, Chung-Ang University

B069

A Case Study on the Dynamics and Resilience of the

Skin Microbiome in Two Healthy Korean Males During

Antarctic Expeditions

Kyu-Chan Lee1, Hanbyul Lee2, Ok-Sun Kim2, and Woo Jun Sul1*

1Department of Systems Biotechnology, Chung-Ang University, 2Division of

Life Sciences, Korea Polar Research Institute

B070

Metabolic Insights and Novel Species Report

Roseovarius and Aliiroseovarius Strains Isolated from

the Marine Conch, Reishia bronni

Seung Hee Ha and Jin-Sook Park*

Department of Biological Sciences and Biotechnology, Hannam University

B071

The Dynamics of Microbial Resuscitation and Functional

Stability in Response to Nutrient Fluctuation

Soo Bin Kim and Tae Kwon Lee*

Department of Environmental and Energy Engineering, Yonsei University

B072

Risk Assessment of Antibiotic Resistance Gene

Dissemination in Cheongju Wastewater Treatment

Plant

Young Jin Cheon1, Geon Choi2, Somi Lee1, and Tatsuya Unno3*

1Department of Microbiology, Chungbuk National University, 2Department

of Environmental and Biological Chemistry, Chungbuk National University,

3Department of Biological Sciences and Biotechnology, Chungbuk National

University

B073

Agrobacterium sp. that Enhance the Activity of a

Woodchip Bioreactor

JinWoo Ahn, Changwoo Lee, and Jeonghwan Jang*

Division of Biotechnology, Jeonbuk National University

B074

Phylogenetic Analysis and Environmental Distribution

of Methane Monooxygenases Highlight Its Functional

and Ecological Significance

Ju Yong Lee and Sukhwan Yoon*

Department of Civil and Environmental Engineering, KAIST

B075

Degradation of the Dicarboximide Fungicide

Procymidone by Rhodococcus YW-5

Jehyeong Yeon, Han Suk Choi, Joon-hui Chung, Young-Joon Ko,

Da-Yeon Kim, Si-Hyun An, Jae-Hyung Ahn, and Hang-Yeon Weon*

Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration

B076

Correlation between Gut Microbiota and Nutritional

Status of Farmed Atlantic Salmon in Korea:

Identification of Potential Probiotics

Mi-Jeong Park1, Min Joo Kang1, Gyeong Hak Han1,2, Jihyun Yu1,2,

Choong Hwan Noh1, and Kae Kyoung Kwon1,2*

1Marine Biotechnology & Bioresource Research Department, Korea Institute

of Ocean Science & Technology, 2Department of Marine Biotechnology,

University of Science and Technology

B077

Degradation of 3,4- and 3,5-Dichloroaniline

Metabolites from Dicarboximide Fungicides by

Pseudomonas putida KACC 10270T

Jehyeong Yeon, Joon-hui Chung, Han Suk Choi, Young-Joon Ko,

Da-Yeon Kim, Si-Hyun An, Jae-Hyung Ahn, and Hang-Yeon Weon*

Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration

B078

Screening of Skin Microbiota for Potential

Anti-Staphylococcus aureus Strains

Chaeryeon Seo and Woo Jun Sul*

Department of Systems Biotechnology, Chung-Ang University

B079

Changes in Microbiota Composition due to Crop

Rotation of Tomatoes and Rice

Hyun Gi Kong1, Ismaila Yakubu1,2, Ji Ho Song1, Min A Son1, Yun Ju

Lee1, Su Hyeon Han1, and Hyun Gi Kong1*

1Department of Plant Medicine, College of Agriculture, Life and Environment

Science, Chungbuk National University, 2Department of Crop Protection,

Faculty of Agriculture/ Institute for Agricultural Research, Ahmadu Bello

University, Zaria 810107 Nigeria

2024 International Meeting of the Microbiological Society of Korea

238 | www.msk.or.kr

B080

Impact of JAK Inhibitors on Skin Mycobiome

Composition in Autoimmune Skin Diseases

Hyerim Do and Woo Jun Sul*

Department of Systems Biotechnology, Chung-Ang University

B081

Epidemiological Surveillance of Carbapenemase-producing

Pseudomonas aeruginosa in Hospital

Su Min Kwak1, Jae Young Oh1, Woo Jun Sul2, Seok Hoon Jeong3,

Dokyun Kim3, and Jong-Chan Chae1*

1Division of Biotechnology, Jeonbuk National University, 2Department of

Systems Biotechnology, Chung-Ang University, 3Department of Laboratory

Medicine, Yonsei University College of Medicine

B082

The Effects of Elevated Atmospheric CO2 and Warming

on Soil Microbial Communities along a Chronosequence

in a Tidal Marsh Ecosystem

Yerang Yang1,2, Genevieve Noyce2, Patrick Megonigal2, Hojeong

Kang3, and Jaehyun Lee1,2*

1Korea Institute of Science and Technology, 2Smithsonian Environmental

Research Center, 3Yonsei University

B083

Exploring Patescibacteria in Wastewater Treatment

Plants: Visualization and Biomass Quantification of

Patescibacteria Using FISH and qPCR

Soyeon Kim and Woo Jun Sul*

Department of Systems Biotechnology, Chung-Ang University

B084

Comparative Analysis of Plastisphere Microbiome of

Biodegradable Plastic, Polyhydroxyalkanoate (PHA)

Kyeongmo Lim1 and Jae-Ho Shin1,2*

1Department of Applied Biosciences, Kyungpook National University,

2NGSCoreFacility, Kyungpook National University

B085

Utilization of the Bio-fenton Reaction for the

Depolymerization of Guaiacylglycerol-β-guaiacyl Ether

and Application of Lignin Extracts Enhanced by

Desemzia sp. Strain C1

Sang hyeok Lee, Yong seok Ko, Kudsia Faisal, and Hor-gil Hur*

Gwangju Institute of Science and Technology

B086

Seasonal Dynamics in the Gut Microbiome of Wild Fish

from the Southern Coast of the Korean Peninsula

Gyeong Hak Han1,2, Jihyun Yu1,2, Mi-Jeong Park1, Min Joo Kang1,

Choong Hwan Noh1, and Kae Kyoung Kwon1,2*

1Marine Biotechnology & Bioresource Research Department, Korea Institute

of Ocean Science and Technology, 2Marine Technology and Convergence

Engineering, KIOST School, University of Science and Technology

B087

Emergence of High-risk Resistant Escherichia coli

Strains from Companion Animals in Korea

Su Min Kwak, Joo Yeol Kim, Jae Young Oh, and Jong-Chan Chae*

Division of Biotechnology, Jeonbuk National University

B088

Function of Bacillus amyloliquefaciens DYC2-1 Strain as

Plant Growth Promoter and Biocontrol Agent

Ji-Hwan Lim, On-Yu Kim, Pyoung Il Kim, and Sun Il Seo*

Center for Industrialization of Agricultural and Livestock Microorganisms

B089

Elucidation of Core Bacterial Communities and Their Metabolic

Characteristics in the Phycosphere of Marine Algae

Jeong Min Kim, Byeong Jun Choi, Hülya Bayburt, and Che Ok Jeon*

Department of Life Science, Chung-Ang University

B090

Higher Biomass and ATP Levels were Observed under

Constant Light Conditions in Nutrient-replete Cultures

of Candidatus Puniceispirillum marinum

Hyun-Myung Oh1 and Ji Hyen Lee2*

1Institute of Liberal Arts Education, Pukyong National University, 2Department

of Pediatrics, Ewha Womans University School of Medicine

B091

Antimicrobial Resistance and Genotypic Characteristics

of Enterococcus faecalis Isolated from Food Products

in Korea

Joo Yeol Kim, Su Min Kwak, Jae Young Oh, and Jong-Chan Chae*

Division of Biotechnology, Jeonbuk National University

B092

Rhizosphere Microbial Shifts Induced by Bacillus

amyloliquefaciens and Salicylic Acid Co-inoculation:

Effects on Plant Nutrient Availability and Growth

Sandamali Harshani Kumari Hathurusinghe1, Kyeongmo Lim1, and

Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University, 2Department

of Integrative Biology, Kyungpook National University, 3NGS Core facility,

Kyungpook National University

Poster

www.msk.or.kr | 239

B094

Isolation and Genomic Characterization of Reductive

Acetogens Isolated from Cow Feces

Ye Jin Gwak and HyoJung Lee*

Department of Biological Science, Kunsan National University

B095

Anaerobic Dilution-to-Extinction Culturing of Human

Gut Bacteria

Minseok Kim1, Hyunyoung Jo2, Ilnam Kang2, and Jang-Cheon Cho2*

1Department of Biological Sciences and Bioengineering, Inha University,

2Department of Biological Sciences and Bioengineering, Inha University

B096

Complete Genome Sequence of a Carotenoid-producing

Bacterium, Pontibacter sp. MBLB2868, Isolated from

Korean Marine Mud Flat

Hyeryeon Gyeong1,2 and Myung-Ji Seo1,2,3*

1Division of Bioengineering, Incheon National University, 2Research Center

for Bio Materials & Process Development, Incheon National University,

3Department of Bioengineering and Nano-Bioengineering, Incheon National

University

B097

Effects of Early Fattening Initiation on the Rumen

Microbial Community in Hanwoo Cattle

Hyeran Kim1, Chaehwa Ryu1, Seol Hwa Park1, Hyun Jung Lee1,

Seon-Ho Kim2, Sang-Suk Lee2, and Sung Dae Lee1*

1Animal Nutrition & Physiology division, National institute of Animal Science,

2Department of Animal Science and Technology, Suncheon National University

B098

Estimation of Antimicrobial Resistance Contamination

from Pig to Human in Swine Farms

Gihan Lee1,2, Jin-Kyung Hong3, Tae Kwon Lee4, and Keunje Yoo1,2*

1Department of Environmental Engineering, Korea Maritime and Ocean University,

2Interdisciplinary Major of Ocean Renewable Energy Engineering, Korea

Maritime and Ocean University, 3Department of Environment and Energy

Engineering, Chonnam National University, 4Department of Environmental

and Energy Engineering, Yonsei University

B099

Isolation and Genome Analysis of Acidithiobacillus sp.

Isolated from South Korea

Giwook Hong1, Jong-Seok Lee2, Moonsuk Hur1, Kyung Jin Lee2,

Junmo Ahn3, Gukhwa Hwang3, Yoonyong Yang3, and Hyo Jung

Lee1*

1Department of Biological Science, Kunsan National University, 2Biological

Resources Assessment Division, National Institute of Biological Resources,

3Department of Resources and Energy Engineering Chonbuk National

University

B100

Microbial Changes Across Frog Development Parallel A

Habitat Shift

YunSeok Jeong and Jin-Woo Bae*

Department of Biology, Kyung Hee University

B101

Genetic and Functional Characterization of a Novel

Class A Extended-spectrum β-Lactamase from an

Environmental Isolate, Scandinavium sp.

Seong-Jun Jo1, Eun-Su Seo1, Ju-Yeon Song2, and Dae-Wi Kim1*

1Department of Life Sciences, Jeonbuk National University, 2Department of

Systems Biology, Division of Life Sciences, and Institute for Life Science and

Biotechnology, Yonsei University

B102

Investigation of Microbial Biomarkers for Early

Detection of Squid Spoilage

Myeongjae Kim1, Woojin Jang2, Woo Jun Sul1*, and Jihyun Lee3*

1Department of Systems Biotechnology, Chung-Ang University, 2Department

of Food Science and Biotechnology, Chung-Ang University, 3Department of

Food and Nutrition, Seoul National University

B103

Methane-utilizing Bacteria: Impacts on Rumen

Fermentation, Methane Reduction, and Microbial

Diversity in Hanwoo Steers

Keun Sik Baik1, Janine I. Berdos1,2, Sang Hoon Na1, Michelle A.

Miguel1, Seon Ho Kim1, A Rang Son1, Mozart Nuzul Aprilliza AM1,

Khanza Syahira Dhia1, and Sang Suk Lee1*

1Department of Animal Science and Technology, Sunchon National University,

2Department of Animal Science, College of Agriculture and Forestry, Tarlac

Agricultural University, Philippines

B104

Effects of Phytogenic Complex Supplementation on

Rumen Microbial Composition in Fattening Hanwoo

Steers

Michelle Miguel1, A Rang Son1, Janine I. Berdos1,2, Seon Ho Kim1,

Ye Pyae Naing1, Jeong Uk Jang1, and Sang Suk Lee1*

1Department of Animal Science and Technology, Sunchon National University,

2Department of Animal Science, College of Agriculture and Forestry, Tarlac

Agricultural University, Philippines

B105

Aerobic Denitrifier Neobacillus sp. OS1-2 Isolated from

the Apple Orchard Soil

ChangWoo Lee, Jinwoo Ahn, and Jeonghwan Jang*

Division of Biotechnology, Jeonbuk National University

2024 International Meeting of the Microbiological Society of Korea

240 | www.msk.or.kr

B106

Comparison of Microbial Composition and Potential

Function of Various Hydrothermal Chimneys in the

Indian Ocean

Jiwon Jeong1,2, Yun Jae Kim1,2, Jihyun Yu1,2, and Kae Kyoung

Kwon1,2*

1Marine Biotechnology & Bioresource Research Department, Korea Institute

of Ocean Science and Technology, 2Department of Marine Biotechnology,

KIOST School, University of Science and Technology

B107

Analysis of Mycobiome in Healthy Individuals and

Ulcerative Colitis Patients

Juhee Kim, Jiwon Bang, Minjeong Kwon, and Yong-Joon Cho*

Department of Integrative Molecular and Biomedical Sciences, College of

Biomedical Sciences, Kangwon National University

B108

Metagenomic Surveillance of Mobile Antibiotic

Resistance through a One Health Approach in South

Korea

HyeonJin Kim, Bo-Yun Choi, Jin Ju Kim, and Woo Jun Sul*

Department of Systems Biotechnology, Chung-Ang University

B109

Isolation and Characterization of Cellulolytic Bacteria

from Peach Orchard Soil

Han Suk Choi, Hang Yeon Weon, Jae-Hyung Ahn, Jehyeong Yeon,

Joon-hui Chung, Da-Yeon Kim, Sihyun An, and Young-joon Ko*

Agricultural Microbiology Division, National Institute of Agricultural Sciences

B110

Spatial and Temporal Changes in Phytoplankton and

Prokaryotic Community Structures during the Austral

Summer in Marian Cove, West Antarctica

Ami Rho1,2, Su Yeon Lee1, Jinyoung Jung2, Jisoo Park2, and Chung

Yeon Hwang1*

1Microbial Oceanography Laboratory, School of Earth and Environmental

Sciences, Research Institute of Oceanography, Seoul National University,

2Division of Ocean and Atmosphere Sciences, Korea Polar Research Institute

B111

Effects of Biodegradable Mulch Film on Soil

Physicochemical Properties and Microbial Communities

under a Laboratory Conditions

Joon-hui Chung, Han Suk Choi, Jehyung Yeon, Yuna Jeon,

Young-Joon Ko, Hang Yeon Weon, Sang-Bum Kim, and Jae-Hyung

Ahn*

Agricultural Microbiology Division, National Institute of Agricultural Sciences

B112

Tyromyces kmetii Extract Inhibits Biofilm Formation by

Pseudomonas aeruginosa PA14

Hyojin Kim, Hee Ho Lee, and Han-Shin Kim*

Division of Biotechnology, College of Environmental and Bioresource Sciences,

Jeonbuk National University

B113

Characteristics of Vancomycin-resistant Enterococcus

faecium - vanA Isolates in South Korea During 2021-2022

Se Kye Kim, Thi Mai Tho Nguyen, Hong Qui Le, Douk Ju Han,

Chinchuluun Boldbataar, and Jang Won Yoon*

College of Veterinary Medicine and Institute of Veterinary Science, Kangwon

National University

B114

Diversity Patterns of Foliicolous Alga Cephaleuros in

Lichenized and Free-living Conditions in South Korea

Seung-Yoon Oh, Yunhyeok Jang, Yehyeon Cha, and Yoonjoo Noh*

Department of Biology and Chemistry, Changwon National University

B115

Microbial Degradation of Polycaprolactone (PCL) by an

Indigenous Marine Bacterium Isolated from a Tidal Flat

Dae Young Kwon, Yeonjae Yoo, Sang Hyun Lee, Minseo Cho, and

Jae-jin Kim*

Division of Environmental Science & Ecological Engineering, College of Life

Science & Biotechnology, Korea University

B116

Monitoring Bacterial Communities in Getbol Soil

Sediments of Gangwha Island

Sang Hyun Lee, Yeonjae Yoo, Dae-Young Kwon, Minseo Cho, and

Jae-Jin Kim*

Division of Environmental Science & Ecological Engineering, College of Life

Science & Biotechnology, Korea University

B117

Environmetal DNA Analysis of Algal and Fungal

Diversity in a Forest Wetland of South Korea

Seung-Yoon Oh1, Yunhyeok Jang1, Yehyeon Cha1, Yoonjoo Noh1,

and Jongbin An2*

1Department of Biology and Chemistry, Changwon National University,

2DMZ Botanic Garden, Korea National Arboretum

B118

Effects of Ulva pertusa Kjellman Extract on Methane

Reduction by Bovine Gut Microbiota

Min-Ju Kang1, Israr Aziz1, and Man-Young Jung1,2*

1Interdisciplinary Graduate Program in Advance Convergence Technology

and Science Jeju National University, 2Department of Biology Education,

Jeju National University

Poster

www.msk.or.kr | 241

B119

Bacterial Community Dynamics during the Red Cyst

Stage of Haematococcus lacustris

Mehwish Taj1, Ve Van Le2, Ji Eun Jeong2, Man-Young Jung1,2,3,4,

and Sang-Ah Lee1,2,3*

1Interdisciplinary Graduate Program in Advanced Convergence Technology

and Science, Jeju National University, 2Faculty of Biotechnology, College of

Applied Life Sciences, Jeju National University, 3Bio-Health Materials

Core-Facility Center, Jeju National University, 4Department of Biology

Education, Jeju National University

B120

Correlation Analysis Using Big Data to Identify

Fusarium Head Blight Disease Causing Factors

Eunji Jeong

School of Systems Biomedical Science, Soongsil University

B121

Elucidation of the Role of the Genus Aeromonas in

Antibiotic Resistance Gene Dissemination Based on

Comparative Genomic Analyses

Sang-Gyu Kim, Dong-Won Lee, and Dae-Wi Kim*

Division of Life Sciences, Jeonbuk National University

B122

Unraveling the Ecosystem Adaptive Strategies of the

Nitrosocosmicus Clade of Ammonia-oxidizing Archaea

Seongwook Kim1, Jong Hee Im2, and Man-Young Jung1,2*

1Interdisciplinary Graduate Program in Advance Convergence Technology

and Science, Jeju National University, 2Department of Biology Education,

Jeju National University

B123

High-throughput Culturomics Approach for Optimizing

Anaerobic Gut Bacteria Cultivation Using a FACS

Seung Min Han1, Kyoung Su Kim1, Jihye Noh2, Hong Koh2, Do Yup

Lee3,4*, and Dong-Woo Lee1*

1Department of Biotechnology, Yonsei University, 2Department of Pediatrics,

Yonsei University College of Medicine, Severance Fecal Microbiota Transplantation

Center, Severance Hospital, 3Department of Agricultural Biotechnology,

Seoul National University, 4Center for Food and Bioconvergence, Research

Institute for Agricultural and Life Sciences, Interdisciplinary Programs in

Agricultural Genomics, Seoul National University

B124

Plant Growth-promoting Properties of Antarctic Plant

Associated Pseudomonas spp.

Yelim Lee1,2, Huiwon Choi1, Hyoungseok Lee1, Youbong Hyun2,

and Jungeun Lee1*

1Division of Life Science, Korea Polar Research Institute, 2Department of

Biological Sciences, Seoul National University

B125

The Cold Adaptation and Diversity of Bacteria Isolated

from the Antarctica

Ji Hyeon Lee1, Ki Hyun Son1, Rye Gyeong Park1, Soo Hyun Han1,

Hyun Cheol Oh2, and Jae Hak Sohn1,3*

1Department of Food Science and Culinary Arts, College of Health and

Welfare, Silla University, 2Institute of Pharmaceutical Research and Development,

College of Pharmacy, Wonkwang University, 3Seafood Research Center,

IACF, Silla University

B126

The Cold Adaptation and Diversity of Fungi Isolated

from the Antarctica

Ji Hyeon Lee1, Rye Gyeong Park1, Ki Hyun Son1, Soo Hyun Han1,

Hyun Cheol Oh2, and Jae Hak Sohn1,3*

1Department of Food Science and Culinary Arts, College of Health and

Welfare, Silla University, 2Institute of Pharmaceutical Research and Development,

College of Pharmacy, Wonkwang University, 3Seafood Research Center,

IACF, Silla University

B127

Analysis of the Gut Microbiome in the Elderly Gangwon

Cohort According to Obesity Status

Si-hyeon Hwang1, Jin-Jae Lee1, Sa Rang Jeong2, So Hyeon Park2,

and Bong-soo Kim1,2*

1Department of Life Science, Multidisciplinary Genome Institute, Hallym

University, 2Korea Nutrition Research Institute, Hallym University

B128

Plant Growth-promoting Activity of Newly Recorded

Species in Korea Isolated from Coastal Halophytes

yongwook Kim, Da som Kim, Ho-Young Shin, and Song-Ih Han*

Department of Microbial Biotechnology, Mokwon University

B129

Assessing the Influence of Anthocyanins on Gut

Microbiota in Diabetic Mice: A Focus on Microbial

Diversity and Function

Se Joung Kim1, Jihyun Lee2, and Woo Jun Sul1*

1Chung-Ang University, 2Seoul National University

B130

Prevalence of Tick-borne Infectous Pathogens in Ticks

Collected from Gyeongju and Yeongcheon, Republic of

Korea

Sung-Kyung Cha, Young-Hee Son, Yun-Ji Park, Da-Young Song,

Seon-Hee Kang, Sa-Ra Kim, and Hye-Jin Jung*

Gyeongsangbuk-do Government Public Institute of Health & Environment

2024 International Meeting of the Microbiological Society of Korea

242 | www.msk.or.kr

B131

Isolation and Characterization of

Phosphate-solubilizing Bacteria from Gotjawal Soil

Hoyoung Shin, dasom Kim, and Song-Ih Han*

Department of Microbial Biotechnology, Mokwon University

B132

Isolation and Characterization of Plant

Growth-promoting Bacteria and Their Effects on the

Halophyte Suaeda japonica

Su Yeon Lee, Ami Rho, Jy Young Choi, Jun Lee, and Chung Yeon

Hwang*

School of Earth and Environmental Sciences and Research Institute of

Oceanography, Seoul National University

B133

Freshwater Algal Blooms and Their Impact on Nitrogen

Cycling and Microbiome Dynamics

Monica Paola Albuja Delgado and Sukhwan Yoon*

Department of Civil and Environmental Engineering, Korea Advanced Institute

of Science and Technology

B134

Geospatial Analysis of Microbial Contamination in

Groundwater for Identifying Risk Zones

Afrin Jahan Acme, Jae Uk Youn, Tae Kwon Lee*

Yonsei University

B135

The Role of Spatial Proximity in Modulating Microbial

Competitive and Cooperative Dynamics

JinGyeong Jeong, Eun Sun Lyou, and Tae Kwon Lee*

Yonsei University

B136

Biodegradation of Terephthalic Acid by

Stenotrophomonas sp. WED208 and Its Potential

Degradation Mechanism

Chunzhi Jin1, Ye Zhuo1,2, and Hyung-Gwan Lee1,2*

1Cell Factory Research Center, Korea Research Institute of Bioscience and

Biotechnology (KRIBB), 2Department of Biotechnology, KRIBB School of

Bioscience, Korea University of Science and Technology (UST)

B137

Comparative Metagenomic Insights into Symbiotic

Microorganisms in Tropical and Subtropical Marine

Sponges

Jeong-A Kim1, Ye JI Lee1,2, Hyun Sung Yang1, Heung Sik Park1,2,

and Jae Kyu Lim1,2*

1Korea Institute of Ocean Science and Technology, 2KIOST School, University

of Science and Technology

B138

Arbuscular Mycorrhizal Fungi and Bacterial Interactions

Shaping Soil Carbon Storage and Emission

So Hee Park, Bo Ram Kang, Min Sung Kim, and Tae Kwon Lee*

Department of Environmental and Energy Engineering, Yonsei University

B139

Genetic and Metabolomic Characterization of Novel

Species Isolated from Marine Sponges: Aquimarina

callyspongiae sp. nov., Aquimarina mycalensis sp. nov.,

Aquimarina discodermiae sp. nov., and Aquimarina

penarica sp. nov.

Yea Lin Moon, Seung-Min Han, and Jin-Sook Park*

Department of Biological Sciences and Biotechnology, Hannam University

B140

Improved Taxonomic Resolution and Insights into

Microbial Communities using Multiple Sequencing

Platforms

Taeho Yun, Min Joon Song, and Sukhwan Yoon*

Korea Advanced Institute of Science and Technology

B141

Spatial Variability of Soil Trapped Methane and

Controlling Factors in Permafrost across Arctic and

Boreal Ecosystems

Jinhyun Kim1, Yongwon Kim2, Sungjin Nam1, Ji Young Jung1, You

Jin Kim1,3, Jeong Ho Hwang4, and Mincheol Kim1*

1Division of Life Sciences, Korea Polar Research Institute, 2International

Arctic Research Center, University of Alaska Fairbanks, Fairbanks, AK, USA,

3Department of Environmental Science and Engineering, Kyung Hee

University, 4Animal Model Research Group, Korea Institute of Toxicology

B142

Sweet Food, Bitter Life: Glucose-mediated Acidic Stress

Compromises the Membrane Integrity in Clinical

Acinetobacter baumannii

Jihyeon Min and Woojun Park*

Korea University

B143

Halotolerant Beneficial Bacteria Alleviate Salinity

Stress in Maize by Modulating Soil Microbiome

Tino Bashizi1, Kyeongmo Lim1, Minsoo Jeong1, and Jae-Ho

Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University, 2Department

of Integrative Biology, Kyungpook National University, 3NGS Core Facility,

Kyungpook National University

Poster

www.msk.or.kr | 243

C001

Role of Gut Microbiota in Mediating PCOS Treatments

with Myo-inositol and Metformin

In-Chul Jeong1, Jee-Won Choi1, and Jin-Woo Bae1,2*

1Department of Biology, Kyung Hee University, 2Department of Biomedical

and Pharmaceutical Sciences, Kyung Hee University

C002

Exploring the Role of Roseburia in Enhancing Muscle

Health and Exercise Capacity in Aging

Vaibhavi Dhond1,2, Hee Jung1, Bao Ngoc Nguyen3, Tigist Bekele2,3,

Junsun Yu1, Myungsuk Kim2,3,4*, and Jiyeon Si1,2,4*

1Natural Product Informatics Research Center, Korea Institute of Science

and Technology (KIST), 2Division of Bio-Medical Science and Technology,

KIST School, University of Science and Technology (UST), 3Natural Product

Research Center, Korea Institute of Science and Technology (KIST),

4Department of Convergence Medicine, Wonju College of Medicine, Yonsei

University

C003

Characterization of Two Novel Straboviridae Phages,

Showing Synergy with Antibiotics and Biofilm Inhibition

against Multidrug-resistant Escherichia coli

Md Shamsuzzaman1,2, Shukho Kim1,2, Yoon-Jung Choi1,2, and

Jungmin Kim1,2*

1Department of Biomedical Science, School of Medicine, Kyungpook National

University, 2Department of Microbiology, School of Medicine, Kyungpook

National University

C004

Effect of Cell-free Supernatant Derived from

Latilactobacillus curvatus M21F011 for Development

of Functional Cosmetics

Yong Min Kwon, Hyein Jin, and Hyun-Ju Hwang*

National Marine Biodiversity Institute of Korea

C005

Preventive Effects of Kimchi-probiotics on Pancreatic

Cancer and Gut Microbiota Modulation in a Mouse

Orthotopic Model

Hee Eun Jo1,2, Subin Kim1, and Misun Yun1*

1Technology Innovation Research Division, World Institute of Kimchi, 2Department

of Biomedical Sciences, Chonnam National University Medical School

C006

Probiotics Reduce Body Fat and Lipid Accumulation by

Enhancing Lipolysis in High-fat Diet Induced Obese

Mice

Hee Eun Jo1,2, Subin Kim1, Namhee Kim1, Se Hee Lee1, and Misun

Yun1*

1Technology Innovation Research Division, World Institute of Kimchi,

2Department of Biomedical Sciences, Chonnam National University Medical

School

C007

Degradation and Assimilation of Polylactic Acid (PLA)

Waste, a Recalcitrant Bioplastic, Using Microorganisms

Gyulim Park, Young Seok Lee, Yedam Kim, Youngsuk Kim, and

Hong-Joo Son*

Department of Life Science and Environmental Biochemistry, Pusan National

University

C008

Supplementation with Heat-killed Akkermansia

muciniphila EB-AMDK19 Counteracts Diet-induced

Overweight in Beagles

Moon-Gi Hong, Dohak Kim, Won-Seok Chung, Jae-Gu Seo, and

Sang-Nam Lee*

R&D Center, Enterobiome Inc.

C009

Polymicrobial Co-cultivation of Core Microbes in

Patients with Ulcerative Colitis Using In Silico Modeling

Young Woo Kim1, Joon-Young Park1, and Bong-Soo Kim1,2*

1Department of Life Science, Multidisciplinary Genome Institute, Hallym

University, 2The Korean Institute of Nutrition, Hallym University

C010

Optimizing Culture Conditions for the Isolation of

Beneficial Bacteria for Carbapenem-resistant

Enterobacteriaceae Colonized Patients

Yu-Jeong An1, Imchang Lee1, Seung Soon Lee2*, and Bong-Soo

Kim1,3*

1Department of Life Science, Multidisciplinary Genome Institute, Hallym

University, 2Division of Infectious Diseases, Department of Internal Medicine,

Hallym University Chuncheon Sacred Heart Hospital, Hallym University

College of Medicine, 3The Korean Institute of Nutirtion, Hallym University

C011

Malate Dehydrogenase-mediated Activation of

Nε-Lysine Acetyltransferase Modulates Metabolic Flux

Geon Kim, Gwanwoo Lee, and Yeong-Jae Seok*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

C012

Purification, Characterization, and Classification of

L-Arabinose-induced α-L-Arabinofuranosidase from a

New Subspecies of Paenibacillus glucanolyticus

Infected in a Plant-pathogen Pythium ultimum

Hyena Lee, Thi Thu Ha Pham, Yoonho Kim, Hanul Kim, Eunkyung

Shin, and Yong-Hak Kim*

Department of Microbiology, Daegu Catholic University School of Medicine

2024 International Meeting of the Microbiological Society of Korea

244 | www.msk.or.kr

C013

Sleep-enhancing Activity of GABA-rich Fermented Pea

Protein Hydrolysate by Utilizing Lactobacillus brevis

SYLB 0016

Yesol Yoo1, Hyowon Lee2, and Jongwon Byun1*

1Healthcare BU, Samyang Foods Co., Ltd, 2Department of Integrated Biomedical

and Life Science, Graduate School, Korea University

C014

Oral Microbiota Analysis Unveils the True Identity of

Seronegative Rheumatoid Arthritis: Bridging the

Diagnostic Gap

Jee-Won Choi1 and Jin-Woo Bae1,2*

1Department of Biology, Kyung Hee University, 2Department of Life and

Nanopharmaceutical Sciences, Kyung Hee University

C015

Plasmid-encoded Antimicrobial Peptides from Baby

Skin-derived LAB for the Inhibition of Staphylococcus

aureus: A Genomic and Biotechnological Study

Ah Rah Jeong1,2, Somi Park1, and Jin Woo Min1*

1Green & Biome Customizing Laboratory, GFC Co., Ltd., 2School of Environmental

Engineering, University of Seoul

C016

Anti-inflammatory Effects of Heat-killed Probiotic in

LPS-stimulated RAW 264.7 Macrophages

Yu Jin Choi, Min Gyeol Kim, and Baul Yang*

Imsil Cheese & Food Research Institute

C017

Intestinal Microbiome Differences between Ulcerative

Colitis Patients and Healthy Individuals in Korea

Jiwon Park1, Do-Hun Gook1, Gyeongjun Yang1, Kwangwon Seo2,

and Wan-Kyu Lee1,2*

1GutBiomeTech Co., Ltd., 2College of Veterinary Medicine, Chungbuk National

University

C018

Urease-producing Bacteria: Isolation, Characterization,

and Carbonatogenesis

Chaemin Sim1 and Youri Yang1,2*

1School of Natural Resources and Environmental Science, Department of

Biological Environment, Kangwon National University, 2Department of Food

Biotechnology and Environmental Science, Kangwon National University

C019

The Biotic-elicitor on Damnacanthus major Calli

Promotes Wound Healing by Accelerating the Migration

of the HaCaT Cells

Jin Hwang, Eun Bi Jang, Mi-ye Kwon, So-yeon Oh, Ho Bong Hyun,

Boram Go, Jong-Du Lee, Weon-Jong Yoon, Young-min Ham, and Ji

gwon Park*

Biodiversity Research Institute, Clean Bio Business Division, Jeju Technopark

C020

Safety Assessment of Lactobacillus casei WK3 Strain

Shown to Reduce Cholesterol and Visceral Fat for Use

as Health Functional Food

Do-Hun Gook1, Jiwon Park1, Miyeon Park1, Kwangwon Seo2, and

Wan-Kyu Lee1,2*

1GutBiomeTech Co., Ltd., 2College of Veterinary Medicine, Chungbuk National

University

C021

Evaluation of Safety and Processing Stability of

Probiotics with Atopic Dermatitis Alleviation and

Anti-aging Function

Li-Ha Kim1,2,3, Jihye Yang1,2,3, So-Yeon Jin1,2,3, Yelin Choi1,2,3, Jae

Yeon Joung3, and Ju-Hoon Lee1,2,3,4*

1Department of Agricultural Biotechnology, Seoul National University, 2Research

Institute of Agriculture and Life Sciences, Seoul National University, 3Center

for Food and Bioconvergence, Seoul National University, 4Department of

Food and Animal Biotechnology, Seoul National University

C022

Fecal Microbiota Transplantation Effects on CRE

Pathogens In Vivo

Hyeon Seop Lee1,2, Hyochan Jang1, Sinseong Park1, Minkyung

Kim1, Fathrinah Binti Kohadie1, and Robert James Mitchell1*

1School of Life Sciences, Department of Biological Sciences, Ulsan National

Institute of Science and Technology, 2Korea Institute for Ceramic Engineering

and Technology (KICET)

C023

Exploring the Relationship between Bitter Taste

Sensitivity and Oral Microbiota Composition

Seoyeon Kim, Jaeyoung Lee, Do-Eun Ko, Hye-Seong Lee*, and

Byoung-Sik Kim*

Ewha Womans University

C024

Novel Bacterial Isolates Inhibiting Carbapenem

Resistance Bacteria from Healthy Human Feces

Sinseong Park, Joy Edet Ekpenyong, Hyeon Seop Lee, Hyochan

Jang, Minkyung Kim, Fathrinah Binti Kohadie, and Robert James

Mitchell*

Department of Biological Science, Ulsan National Institute of Science and

Technology

Poster

www.msk.or.kr | 245

C025

Synergistic Antifungal Activities of Cell-free

Supernatant and Natural Products against Botrytis

cinerea

Gwon Mo Yang, Na Yun Kim, Hea Joon Kim, So Young Park, Yo Han

Song, Dong Hwa Chung*, and Hyo Jin Kim*

Graduate School of International Agricultural Technology, Seoul National

University

C026

Methionine-(R)-Sulfoxide Reductase MsrB Regulates

Lactate Oxidase of Desemzia sp. Strain C1

Thi Thu Ha Pham1, Yongseok Ko2, Vu Hanh Dung Hoang1,

Yong-Hak Kim1, and Ho-Gil Hur2*

1Department of Microbiology, Daegu Catholic University School of Medicine,

2School of Earth Sciences and Environmental Engineering, Gwangju Institute

of Science and Technology (GIST)

C027

Integrated Transcriptomic and Lipidomic Analysis to

Investigate Lipidotoxic Stress Response in the SUR2

Deletion Strain of the Oleaginous Yeast Yarrowia

lipolytica

Seo Hyeon Shin, Hye Yun Moon, and Hyun Ah Kang*

Department of Life Science, Chung-Ang University

C028

Optimization of IAA Production and Gene Expression

Analysis of Bacillus toyonensis WS2-2 Isolated from

Abelia sphatulata

Seung Hee Ham, Min Ju Kim, Ha Yeon Byun, Gi Heum Nam, and

Youn-Bong Ku*

National Institute of Biological Resources

C029

Probiotic Characteristics of Bacillus subtilis Isolated

from Soybean Fermented Paste (Cheonggukjang): A

Preliminary In Vitro Study

Jegadeesh Raman

Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration

C030

Characterization of the Probiotic Properties of Lactic

Acid Bacteria Isolated from Korean Fermented Starter,

Nuruk

Jegadeesh Raman

Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration

C031

Gut Microbiome Therapy: Fecal Microbiota

Transplantation vs Live Biotherapeutic Products

Do Yeon Kim1, So-Yeon Lee1, Jae-Yun Lee1, Tae Woong Whon3,

June-Young Lee1,2, Che Ok Jeon4, Jin-Woo Bae1,2*

1Department of Life and Nanopharmaceutical Sciences and Department of

Biology, Kyung Hee University, 2Department of Biomedical and Pharmaceutical

Sciences, Kyung Hee University, 3Microbiology and Functionality Research

Group, World Institute of Kimchi, 4Department of Life Science, Chung-Ang

University

C032

Screening of Plant Growth-promoting Rhizobacteria

(PGPR) against Potato Common Scab Pathogen

Streptomyces scabiei

Gyeryeong Bak, Yang-Min Kim, and Jeong-Tae Lee*

Highland Agriculture Research Institute, Natioinal Institute of Crop Science

C033

Isolation of Diverse Laccase-producing Bacteria from

Neutral and Alkaline pH Environments

Miso Kim1, Haeun Kim2, and Youri Yang1,2*

1Department of Food Biotechnology and Environmental Science, Kangwon

National University, 2School of Natural Resources and Environmental Science,

Department of Biological Environment, Kangwon National University

C034

Inducing Dysbiosis in Resident Soil Microbiota Improves

Plant Health by Enhancing the Activities of Exotic

Microbes in Cucumber Rhizosphere

Andargie Yohannes Ebabuye1 and Jae-Ho Shin2,3,4*

1Department of Applied Biosciences, Kyungpook National University, 2Department

of Applied Biosciences, Kyungpook National University, 3Department of

Integrative Biology, Kyungpook National University, 4Next Generation Sequencing

(NGS) Core Facility, Kyungpook National University

C035

Probiotic Potential of Saccharomyces cerevisiae

CACC699 and Its Effect on Growth and Microbiome in

Finishing Pigs

Sun-Bong Choe1,2, Seoyun Son1,2, Soyeon Park1, Dae-Hyuk

Kim1,2,3, and Yangseon Kim1*

1Department of Research and Development, Center for Industrialization of

Agricultural and Livestock Microorganisms, 2Department of Bio-Convergence

Science, Jeonbuk National University, 3Department of Molecular Material

Sciences, Jeonbuk National University

C036

Development of a Method for Detecting Both

Microbiome and Parasites in Pets

Jaehoon Lee, Gyeochang Hong, Hyeri Kim, and Sun Jae Kwon*

AccuGene, Inc.

2024 International Meeting of the Microbiological Society of Korea

246 | www.msk.or.kr

C037

Novel Next-generation Probiotics Exerts

Anti-colorectal Cancer Activity via Butyrate

Eunseo Heo1,2, Byeong Seob Oh1, Seoung Woo Ryu1, Seung Yeob

Yu1,3, Jeong Eun Bak1, and Ju Huck Lee1,2*

1Korean Collection for Type Cultures, Korea Research Institute of Bioscience

and Biotechnology, 2University of Science and Technology (UST), 3BioMedical

Sciences Graduate Program (BMSGP), Chonnam National University

C038

Probiotic Characterization of Enterococcus faecium

Strains Isolated from Feline Feces

EunRyul Oh, Sunjoon Lee, Sun-Bong Choe, Jun-Hyeong Seo, and

Hyun-Jun Jang*

Department of Research and Development, Center for Industrialization of

Agricultural and Livestock Microorganisms

C039

Exploration of the Gut Health Advantages of

Fermented Soybean Meal Products Using the CJ BIO

Animal Nutrition & Health (ANH) Application Platform

Ju Kyoung Oh, Ha-Jung Lee, Min Ah Park, and Jun-Ok Moon*

Application Center, Animal Nutrition, BIO, CJ Blossom Park

C040

Probiotic Characteristics and Application in Panax

ginseng C.A. Meyer of Lactic Acid Bacteria (LAB)

Isolated from Jinan-gun

Sanghyeob Sim, Soo-Chang Na, Dae-Woon Kim, and Tae-Young

Kim*

Institute of Jinan Red Ginseng

C041

Complete Genome Sequencing and Comparative

Analysis of Lacticaseibacillus paracasei FMT2: A

Promising Probiotic Strain

Amani Sliti1, Ryeong Hui Kim2, Dokyung Lee2, and Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University, 2NGS

Core Facility, Kyungpook National University, 3Department of Integrative

Biotechnology, Kyungpook National University

C042

Treatment with Saccharomyces cerevisiae Strain

Suppresses Antibiotics-induced Clostridioides difficile

Infection in a Murine Model

Hoyeon Kim1, Eunjung Kim2, DongHyeon Lee1, DongYeol Kim1, and

Hongseop Yoon1*

1Department of Biology, Handong Global University, 2Handong Global

University

C043

Genomic Features, Aroma Profiles, and Potential as

Starter Cultures of Vinegar Yeast Zygosaccharomyces

bailii

YongUk Cho1, Su Jin Yoo1, Che Ok Jeon1, Seong-Il Eyun1, Jung Ah

Seo2, Min Ju Kim2, and Hyun Ah Kang1*

1Department of Life Science, Chung-Ang University, 2Department of Life

Science, Soong-Sil University

C045

Microbial Diversity: A Key Factor in Powdery Mildew

Resistance in Cucumber

Minsoo Jeong1, Sook-Min Kwon1, and Jae-Ho Shin1,2*

1Department of Applied Biosciences, Kyungpook National University, 2NGS

Core Facility, Kyungpook National University

C046

Enhancing Effect of Enterococcus faecium and

Lactobacillus gasseri on Gut Barrier Integrity in

Colitis Mice

Hoyeon Kim1, Haeun Kim1, Yeoun Kim1, Eunjung Kim2*, and

Hongsuop Yoon1*

1Department of Life Science, Handong University, 2Handong University

C047

A Machine Learning-based Study Disease and Oral

Microbiome

Jaewon Lee, Sunjae Kwon, Banseok Lee, Jaehoon Lee, Hyeri Kim,

and Kwangmoon Cho*

Accugene

C048

Study on the Differences in Soil Bacterial Communities

Related to Ginseng Rusty Root (GRR) in the Republic of

Korea

Sook-Min Kwon1, Muhyeok Kwon1, Yujin Hyun1, Heejoo Hwang1,

Yeong-Jun Park2, Min-Kyu Park2,3, and Jae-Ho Shin1,2*

1Department of Integrative Biology, Kyungpook National University, 2NGS

Core Facility, Kyungpook National University, 3Microbalance Inc.

Poster

www.msk.or.kr | 247

C049

Functional Characterization of Fusarium graminearum

DES1 to Engineer Saccharomyces cerevisiae for the

Production of Human-type Ceramides and Long-chain

Bases

Gijeong Nam, Hye Yun Moon, and Hyun Ah Kang*

Department of Life Science, Chung-Ang University

C050

Effects of Natural Green Tea Powder Compound

Supplementation on Jejunum Mucosa-associated

Microbiota in Weaning Piglet Diets with 16S rRNA

Metagenomic Analysis

Jungwoong Kim, Jeonghwa Jang, Jong Pyo Chae, and Jun-Ok

Moon*

Application Center, Animal Nutrition BIO, CJ Blossom Park

C051

Enhancing Gut Microbiome Diversity and Achieving

Symptom Remission in CDI through FMT

Heejoo Hwang1, HyunWoo Son2, EunSoo Kim3*, and Jae-Ho

Shin1,2,4*

1Department of Integrative Biology, Kyungpook National University, 2Department

of Applied Biosciences, Kyungpook National University, 3Department of

Internal Medicine, School of Medicine, Kyungpook National University, 4Next

Generation Sequencing (NGS) Core Facility, Kyungpook National University

C052

Antioxidant and Anti-inflammatory Properties of

Postbiotics Containing L-Tryptophan-producing

Corynebacterium glutamicum

Hanwool Kim, Min Jeong Gu, Jieun Kim, Yuji Shin, Eunseon Oh,

Jong Pyo Chae, and Jun-Ok Moon*

Application Center, Animal Nutrition BIO, CJ Blossom Park

C053

Screening of Beneficial Bacteria with Antifungal

Activities from Soil and Roots for Pinus Species

Jiwon Kim, A Yeong Heo, Yeonghwan Jeong, Ki Hyeong Park, and

Sang-Tae Seo*

Forest Entomology and Pathology Division, National Institute of Forest

Science

C054

Evaluation of Extracellular Vesicles Derived from

Saccharomyces cerevisiae on Factors Related to Skin

Barrier

Iljee Cho, Eunji Kim, Seunghee Lee, and Eunmi Choi*

R&D Center, RADIANT Inc.

C055

The High-throughput Solid-phase Extraction of

cis-cyclo(L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro)

from Lactobacillus plantarum Exhibits Effectiveness

against Multidrug-resistant Bacteria and the Influenza

A Virus

Min-Kyu Kwak, DaGyeong Jung, Hyeri Seong, Jaeyoung Son,

Yeonju Hong, and Min-Kyu Kwak*

Laboratory of Microbial Physiology and Biotechnology, Department of Food

and Nutrition, Institute of Food and Nutrition Science, College of

Bio-Convergence, Eulji University

C056

Classification and Functional Characterization of

Chitinases from Debaryomyces hansenii as Potential

Novel Biological Control Agents

Daeun Kim, Su Jin Yoo, Seong-Il Eyun, and Hyun Ah Kang*

Department of Life Science, Chung Ang University

C057

Evaluation of Antibacterial and Antioxidant Activities

of Bifidobacterium Cell-free Supernatant

Naeun Kim and Hakdong Shin*

Department of Food Science & Biotechnology, and Carbohydrate Bioproduct

Research Center, Sejong University

C058

Differential Impact of Biofumigation with Brassica

juncea on Soil Microbial Communities: Effects of

Glucosinolate Presence in Topsoil and Subsoil

Muhyeok Kwon1, Sihyun Park1, Tino Bashizi2, and Jae-Ho Shin1,2,3*

1Department of Integrative Biology, Kyungpook National University, 2Department

of Applied Biosciences, Kyungpook National University, 3NGS Core Facility,

Kyungpook National University

C059

Impact of Monosaccharide Supplementation on Gut

Microbiota: Enhancing Beneficial Bacteria Growth

Taehun Roh and Hakdong Shin*

Department of Food Science & Biotechnology, and Carbohydrate Bioproduct

Research Center, Sejong University

C060

Enhancing Alcohol Metabolism through Leuconostoc

mesenteroides VITA-PB2: A Potential Solution for

Alcohol-induced Toxicity

Yun Ji Choi1 and Man-Young Jung1,2*

1Interdisciplinary Graduate Program in Advance Convergence Technology

and Science, 2Department of Biology Education, Jeju National University

2024 International Meeting of the Microbiological Society of Korea

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C061

Distinct Gut Microbial Signatures and Predictive Metabolic

Pathway Changes in Irritable Bowel Syndrome

Donghyun Noh1, Gyungcheon Kim1, Cheol Min Shin2, Hakdong

Shin1*

1Department of Food Science & Biotechnology, and Carbohydrate Bioproduct

Research Center, Sejong University, 2Department of Internal Medicine, Seoul

National University Bundang Hospital

C062

Evaluation of Antibacterial Potential of Postbiotics: A

Study on Heat-treated Metabolite of Lactobacillus

Strains

Jaehyuk Jang and Hakdong Shin*

Department of Food Science & Biotechnology, and Carbohydrate Bioproduct

Research Center, Sejong University

C063

Comparative Genomic Characterization of the Food Spoilage

Pseudomonas putida Specific Phages YH06 and YH07

Yonghyun Lee and Minsik Kim*

Department of Food and Nutrition, College of Human Ecology, Yonsei

University

C064

In-depth Investigation of the Infection Mechanism of

Acinetobacter-specific Tectivirus Phage

Jae Hyung Kim and Minsik Kim*

Department of Food and Nutrition, College of Human Ecology, Yonsei University

C065

Bacteriophage-derived Putative Depolymerases Active

against Capsular Polysaccharides of Klebsiella

pneumoniae

Miri Kwon and Minsik Kim*

Department of Food and Nutrition, College of Human Ecology, Yonsei University

C066

Evaluation of Extracellular Vesicles Derived from

Lactobacillus plantarum on Factors Related to Skin

Barrier

Seung Hee Lee, Iljee Cho, Eunji Kim, and Eunmi Choi*

R&D Center, RADIANT Inc.

C067

Effective Control of Soft Rot Disease Caused by Gram-

negative Pectobacterium spp. with AMP-fused PlyPw

Seohyun Nam and Minsik Kim*

Department of Food and Nutrition, College of Human Ecology, Yonsei

University

C068

Multifunctional Properties of Bacillus subtilis NM4 and

Its Control Efficacy on Grape Anthracnose

Seo Hyun Hwang, Chaw Ei Htwe Maung, Jun Su Noh, Seong Eun

Han, and Kil Yong Kim*

Department of Agricultural Chemistry, Environmentally-Friendly Agricultural

Research Center, College of Agriculture and Life Sciences, Chonnam

National University

C069

Characterization of Enterococcus faecium Phage

vB_EfaS_KS1 and Enteroccocus faecalis Phage

vB_EfaS_KS2

Kang Seok Choi and Minsik Kim*

Department of Food and Nutrition, College of Human Ecology, Yonsei

University

C070

Genomic Analysis and Characterization of Bacteriophage

UP01 Targeting Pseudomonas aeruginosa

Hyeiweon Choi and Minsik Kim*

Department of Food and Nutrition, College of Human Ecology, Yonsei

University

C071

Inhibitory Effects of Maillard Reaction Products from

Soy Protein by Lacticaseibacillus rhamnosus

IM18-mediated Fermentation in Gardnerella

vaginalis-induced Bacterial Vaginosis Mouse Model

Hyunjin Bae and Namsu Oh*

Department of Food and Biotechnology, Korea University

C072

Evaluation of Potential Preventive Effects of

Para-probiotics on Immune System and Skin Aging

HaeRyn Jeong, JiangYu Li, SoJeong Lim, and NamSu Oh*

Department of Food and Biotechnology, Korea University

C073

Therapeutic Potential of Lacticaseibacillus rhamnosus

BELR47 in a Bacterial Vaginosis Mouse Model

Donghwan Lee and Namsu Oh*

Department of Food and Biotechnology, Korea University

C074

Exploring Candidate Microbial Strains for Enhanced

Calcite Precipitation Technology

Ha-Yeon Song, Seobeen Jo, Jihun Kim, and Jung-Mi Kim*

Department of Life and Environmental Sciences, Institute of Life Science

and Natural Resources, Wonkwang University

Poster

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C075

Optimized Growth Medium for Enhanced Mycelial

Inoculum Production in Sawdust-based Cultivation of

Lentinula edodes

Ha-Yeon Song1, Seobeen Jo1, Dae-Hyuk Kim2, and Jung-Mi Kim1*

1Department of Life and Environmental Sciences, Institute of Life Science

and Natural Resources, Wonkwang University, 2Department of Molecular

Biology, Department of Bioactive Material Sciences, Institute for Molecular

Biology and Genetics, Chonbuk National University

C076

Evaluation of Spoilage-related Bacterial Profile of

Vacuum-packaged Chilled Pork by Next-Generation

DNA Sequencing Approach

Bu-Min Kim, Eun-Seon Lee, and Mi-Hwa Oh*

National Institute of Animal Science, Rural development Administration

C077

Alleviating Effects of Korean Red Ginseng on Dextran

Sodium Sulfate-induced Colitis through Modulation of

Intestinal Microbiota in Mice

Suyeon Yoo, Do Young Jin, Dongsu Kang, Gyungnam Go,

Chaehyun Kim, Taewan Kim, and Jun-Seob Kim*

Department of Nano-Bioengineering, Incheon National University

C078

The Population of Enterococcus in the Intestine of

Galleria mellonealla Influences Its Growth

Do young Jin, Suyeon Yoo, and Jun-Seob Kim*

Department of Nano-Bioengineering, Incheon National University

C079

Optimal Selection of Bacteria-mediated Delivery to

Enhance Therapeutic Efficacy in Breast Cancer Models

Jam-Eon Park1,2, Seung-Hyon Choi1, Ju Huck Lee1, Jung-Sook

Lee1, Ji-Sun Kim1*, and Seung-Hwan Park1*

1Biological Resource Center, Korea Research Institute of Bioscience and

Biotechnology, 2Department of Molecular Medicine, Chonnam National University

C080

Gut Microbiome–Creatine Synergistic Effects against

Obesity

Su-Won Jeong1, Jeong-Eun Han1, and Jin-Woo Bae1,2*

1Department of Biology, College of Science, Kyung Hee University, 2Department

of Biomedical and Pharmaceutical Sciences, Kyung Hee University

C081

Metagenomic Profiling of Age-related Changes in Facial

Skin Microbiome

Ikwhan Kim1, Da-Ryung Jung2, YeonGyun Jung3, Yoon Soo Cho4,

and Jae-Ho Shin1,2,5*

1Department of Integrative Biology, Kyungpook National University, 2Department

of Applied Biosciences, Kyungpook National University, 3Burn Institute,

Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital,

Hallym University College of Medicine, 4Department of Rehabilitation

Medicine, Hangang Sacred Heart Hospital, Hallym University College of

Medicine, 5NGS Core Facility, Kyungpook National University

C082

Enhancing Gut Health: The Impact of Seed Mucilage

Prebiotics on Infant and Adult Microbiome

Kanika Mahra1, Vineet Singh1, and Jae-Ho Shin1,2,3*

1Department of Applied Biosciences, Kyungpook National University, 2Department

of Integrative Biology, Kyungpook National University, 3Next Generation

Sequencing (NGS) Core Facility, Kyungpook National University

C083

Interplay between Vaginal Microbiome and High-risk

HPV in Cervical Dysplasia

Da-Ryung Jung1, Ye Seul Choi2, Minsoo Jeong1, Vineet Singh1, Se

Young Jeon3, Hyung Soo Han2, Gun Oh Chong3, and Jae-Ho

Shin1,4*

1Department of Applied Biosciences, Kyungpook National University,

2Department of Biomedical Science, Graduate School, Kyungpook National

University, 3Department of Obstetrics and Gynecology, Kyungpook National

University Chilgok Hospital, 4NGS Core Facility, Kyungpook National University

C084

The Impact of Gut Microbiome on Type 2 Diabetes

Improvement: Insights from Roux-en-Y Reconstruction

after Gastrectomy

GyuDae Lee1, Min-Ji Kim1, HeeJoo Hwang2, JiYeon Park3, and

Jae-Ho Shin1,2,4*

1Kyungpook National University, Department of Applied Biosciences, 2Department

of Integrative Biotechnology, Kyungpook National University, 3Department

of Surgery, School of Medicine, Kyungpook National University, Kyungpook

National University Chilgok Hospital, 4NGS Core Facility, Kyungpook National

University

2024 International Meeting of the Microbiological Society of Korea

250 | www.msk.or.kr

D001

Identification and Development of Novel Synthetic

Molecules Targeting Bacterial Proton Motive Force

Jinbeom Seo, Ji-Hoon Kim, Subin Hong, Jihyeon Kim, Seonmin Lee,

and Wonsik Lee*

School of Pharmacy, Sungkyunkwan University

D002

Identification of Metabolic Changes in Staphylococcus

aureus upon Exposure to Aminoglycosides and

Quinolones

Inseo Kim, JuOae Chang, YuJin Shin, Taegwan Yun, Hyesoo Kwon,

and Wonsik Lee*

School of Pharmacy, Sungkyunkwan University

D003

Serotype, Virulence Gene and Antimicrobial Resistance

of Salmonella Isolated from Duck Carcass of

Slaughterhouses

Hye-Rin Lee, Jiyeon Jeong, Hye-Ri Jung, Jin-San Moon, Min-Su

Kang, Moon Her, and Myeongju Chae*

Avian Disease Research Division, Animal and Plant Quarantine Agency

D004

Occurrence and Characteristic Analysis of Major

Diseases in Apples in the Gyeongsangnam-do

Inyoung Han1, Eunsol Kang1, Dong-Wan Kang2, Young-Do Chin2,

and Jae-Hyeok Choi2*

1Gyeongsangnam-do Agricultural Research & Extension Services, 2Agricultural

Environment Division, Gyeongsangnam-do Agricultural Research and Extention

Services

D005

oipA Phase Variation Changes Host Morphology by

Indirectly Regulating CagA Phosphorylation in

Helicobacter pylori

Myeong-A Kim1, Jing Lai1,2, Sacheera Angulmaduwa1, Aeryun

Kim1,3, Kavinda Tissera1,4, Yong-Joon Cho5, and Jeong-Heon Cha1*

1Department of Oral Biology, Oral Science Research Center, Department of

Applied Life Science, The Graduate School, BK21 FOUR Project, Yonsei

University College of Dentistry, 2Department of Periodontics, Changsha

Stomatological Hospital, Changsha, 410008, Hunan, China, 3Oral Health

Research Institute, Apple Tree Dental Hospital, 4Department of Medical

Laboratory Science, Faculty of Allied Health Sciences, University of Peradeniya,

Peradeniya 20400, Sri Lanka, 5Department of Molecular Biosciences,

College of Biomedical Sciences, Kangwon National University

D006

Compound V46 Promotes Host Immune Pathways to

Protect against Mycobacteroides abscessus Infection

through the Activation of Autophagy and Transcription

Factor EB

Eun-Jin Park1,2, Asmita Sapkota1, Young Jae Kim1,2,3, Gyu Yong

Song4, Jichan Jang5, and Eun-Kyeong Jo1,6*

1Department of Microbiology, College of Medicine, Chungnam National

university, 2Brain Korea 21 FOUR Project for Medical Science, College of

Medicine, Chungnam National University, 3Department of Medical Science,

Chungnam National University College of Medicine, 4College of Pharmacy,

Chungnam National University, 5Division of Life Science, Department of Bio

& Medical Big Data (BK21 Four Program), Research Institute of Life Science,

Gyeongsang National University, 6Infection Control Convergence Research

Center, Chungnam National University College of Medicine

D007

Discovery of a Novel Transcription Factor Regulating

the Virulence of Vibrio vulnificus

Dayoung Sung and Sang Ho Choi*

National Research Laboratory of Molecular Microbiology and Toxicology,

Department of Agricultural Biotechnology, Center for Food and Bioconvergence,

Seoul National University

D008

Discovery of a Small Molecule X Inhibiting TF, the

Transcriptional Regulator for Biofilm Formation of

Vibrio vulnificus

Hojun Lee, Gaeun Lee, and Sang Ho Choi*

National Research Laboratory of Molecular Microbiology and Toxicology,

Department of Agricultural Biotechnology, Seoul National University

D009

α-Hemolysin Plays a Crucial Role in Nullifying

Acidification of Bacteria Harboring Lysosomes during

Uropathogenic E. coli Infection in Bladder

Manisha Naskar1, Viraj Parekh2, Soman Abraham2, and HaeWoong

Choi1*

1Molecular Biology, Korea University, 2Biochemistry, Duke University Medical

Center, Durham 27710, USA

D010

Investigation on the Presence of Carbapenem-resistant

Enterobacterales (CRE) and Carbapenemase-producing

Enterobacterales (CPE) from Sewage in Ulsan

DaeKyo Kim1, doHun Gong2, HyeKyeong Moon2, JeongYun

Hwang2, JongSeok Oh2, JiHye Shin2, SeonHwa kim2, KyeongSim

Park2, and YeongSeon Choi2*

1Infection Disease Division, Ulsan Research Institute of Public Health and

Environment, 2Ulsan Research Institute of Public Health and Environment

Poster

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D011

Evolutionary Unique N-Glycan-dependent Glycoprotein

Quality Control System Plays Critical Roles for Extracellular

Vesicle Trafficking in Cryptococcus neoformans

Catia Mota, Ye Ji Son, Kiseung Kim, Eun Jung Thak, Su-Bin Lee,

and Hyun Ah Kang*

Department of Life Science, Chung-Ang University

D012

Quorum Sensing and Its Influence on the Pathogenicity

and Survival of Burkholderia plantarii

Minhee Kang, Duyoung Lee, Seungchul Lee, Taeho Jeong, Haeun

Choi, Seokhun Jang, and Young-Su Seo*

Department of Integrated Biological Science, Pusan National University

D013

Evaluation of the Pathogenic Potential of Newly Isolated

Klebsiella oxytoca Strain Capable of Inducing Inflammation

Tae-Hwan Kim

College of Veterinary Medicine, Chungnam National University

D014

Replicating the Human Gut: An In Vitro Co-culture

System for Host and Anaerobic Microbial Interaction

Chan-hyeok Park1, Haneol Yang1, Hana Jo1, Ye Seul Son2, Naeun

Son2, Mi-Young Son2,3, and Doo-Sang Park1,3*

1Korean Collection for Type Cultures, Biological Resource Center, Korea

Research Institute of Bioscience and Biotechnology (KRIBB), 2Stem Cell

Research Convergence Center, KRIBB, 3KRIBB School of Bioscience, Korea

University of Science and Technology (UST)

D015

Phylogenetic Evolutionary Analysis and Distribution of

Culex flavivirus from Culex pipiens Complex in

Chungcheongnam-do, 2023

Dong Uk Kim, Hye lin Kim, Lee Song, and Hyeon Jeung Kim*

Chungcheongnam-do Health & Environmental Research Institute

D016

The Immunogenicity of an Inactivated SFTSV Vaccine in

Dogs

Da-eun Jeong1 and Jun-gu Kang1,2*

1Jeonbuk National University, 2Korea Zoonosis Research Institute

D017

Characteristics of ESBL-Producing Salmonella spp.

Isolated from Diarrheal Patients

Bu-Geon Lim*, Kyung-A Kim, Min-Seong Kang, Chan-Mi Lee,

Jeong-Hwa Park, and Su-Hyun You

Microbiological Inspection Team, Northern Branch Division, Gyeonggi Province

Institute of Health and Environment

D018

Comparative Proteomic Analyses for a Putative

Carbamoyl Phosphate Synthetase Required for

Virulence and Biosynthesis of Arginine and Pyrimidine

in Erwinia amylovora

Yoobin Han1, Seung Yeup Lee2, Junhyeok Choi1, Yongmin Cho1,

Jeongwook Lee1, Eunjung Roh2, and Sang-Wook Han1*

1Department of Plant Science and Technology, Chung-Ang University, 2Crop

Protection Division, National Institute of Agriculture Sciences, Rural Development

Administration

D019

Gut Microbiome as a Biomarker for CAR-T Cell Therapy

Outcomes in Relapsed/Refractory Diffuse Large B-Cell

Lymphoma

Woorim Kang1, Sang Eun Yoon2, Junhun Cho3, Jae-Yun Lee4,

Hojun Sung4, Seok Jin Kim2, and Won Seog Kim2*

1CJ Bioscience, 2Division of Hematology-Oncology, Department of Medicine,

Samsung Medical Center, Sungkyunkwan University School of Medicine,

3Department of Pathology, Samsung Medical Center, Sungkyunkwan University

School of Medicine, 4Department of Biology and Department of Life and

Nanopharmaceutical Sciences, Kyung Hee University

D020

Environmental Surveillance of Legionella Species in

Public Facilities Busan, 2023

Mijung Park, HyeSook Heo, JiHye Kang, and YonKoung Park*

Microbiology Team, Busan Metropolitan City Institute of Health & Environment

D021

A Survey on the Contamination of Pathogenic Bacteria

in 119 Ambulances in Busan

Sohyun Park, Mi Jung Park, Ji Yoon Lee, and Yon Koung Park*

Microbiology Team, Busan Metropolitan City Institute of Health & Environment

D022

Case Report of Necrotic Hepatitis Caused by

Clostridium perfringens Type A in Broilers

Yesong Kim, Jae-Kyeom Kim, Chi Sun Yun, Moon Her, and Jin-San

Moon*

Avian Disease Division, Animal and Plant Quarantine Agency

D023

Differential Expression of Virulence-associated Genes

in Hybrid Escherichia coli during HeLa Cell Invasion

Gaeun Lee, Hojun Lee, and Sang Ho Choi*

National Research Laboratory of Molecular Microbiology and Toxicology,

Department of Agricultural Biotechnology, Seoul National University

2024 International Meeting of the Microbiological Society of Korea

252 | www.msk.or.kr

D024

Potential Probiotic Lactiplantibacillus plantarum

DS1800 Extends Lifespan and Enhances Stress

Resistance in Caenorhabditis elegans Model

Seunghyun Kim1, Yu-Ri Lee1, Chan-Hyeok Park1, Byung-Chun

Jang1, Haneol Yang1, Chan-Seok Yun1, and Doo-Sang Park1,2*

1Korea Research institute of Bioscience and Biotechnology, 2Korea University

of Science and Technology

D025

Lycosin-II Exhibits Antifungal Activity and Inhibits

Dual-species Bioﬁlm by Candida albicans and

Staphylococcus aureus

Yoonkyung Park1,2,3

1Department of Integrative Biological Science & BK 21 FOUR Educational

Research Group for Age-Associated Disorder Control Technology, Chosun

University, 2Department of Biomedical Science, College of Natural Sciences,

Chosun University, 3Research Center for Proteineous Materials (RCPM),

Chosun University

D026

Evaluating the Efficacy of Streptococcus bovis/equinus

Complex (SBSEC) Lytic and Lysogenic Bacteriophages

in the Ruminants Derived Sources

Seon Young Park1, Ye Bin Kim2, Seung Hui Lee2, Seongwon Seo1*,

and Ji Hyung Kim2*

1Chungnam National University, 2Gachon University

D027

Changes in the Gut Microbiota Caused by Radioactive

Iodine Treatment in Mice

Keun-Woo Lee, Se Jong Oh, Jin-Hee Seo, Chanwoo Park, Jisu Sun,

Sujin Lee, Jang Woo Park, Yong Jin Lee, and Hye Kyung Chung*

Korea Radioisotope Center for Pharmaceuticals, Korea Institute of Radiological

& Medical Science

D028

Antifungal Activities of Cell Free Supernatant Produced

by Lactic Acid Bacteria Isolated from Korean

Traditional Food

Na Yun Kim, Gwon Mo Yang, and Hyo Jin Kim*

Graduate School of International Agricultural Technology, Seoul National

University

D029

Effect of Oral Administration of Lactobacillus

plantarum KF511 on Gut Microbiome in COPD Mice

Yongsoo Park and Mi Young Lim*

Personalized Diet Research Group, Korea Food Research Institute

D030

In Vitro Simulation of Shiga Toxin-producing E. coli

Infection Response Using a Co-culture Model of Porcine

Intestinal Epithelial Cells and Immune Cells

Hyungjun Choi, Min Jeong Gu, Eunseon Oh, Jong Pyo Chae, and

Jun-Ok Moon*

Application Center, Animal Nutrition BIO, CJ Blossom Park

D031

Isolation and Characterization of Acute

Hepato-pancreatic Necrosis Disease

(AHPND)-associated Mutant Vibrio parahaemolyticus

from Penaeus vannamei Cultured in Korea

Ye Bin Kim1, Seon Young Park2, Jee Eun Han3, Seung Hui Lee1, and

Ji Hyung Kim1*

1Department of Food Science and Biotechnology, College of BioNano

Technology, Gachon University, 2Division of Animal and Dairy Sciences,

College of Agriculture and Life Science, Chungnam National University,

3Institute for Veterinary Biomedical Science, College of Veterinary

Medicine, Kyungpook National University

D032

Effect of Combined Use of Anthranilate and Antibiotics

on Pseudomonas aeruginosa Infection

Wenxin Niu, Min-Hee Kang, Huiyan Li, Hyeon-Ji Hwang, and

Joon-Hee Lee*

Department of Pharmacy, College of Pharmacy, Pusan National University

D033

The Significance of KMRC’s Clinical Isolates in

Collaborative Studies on Bacterial Factors in

Nontuberculous Mycobacterial Pulmonary Disease

(NTM-PD)

Minji Kang1, Eun Ju Lee1, Jeong Seong Yang1, Yumi Park1, Da-Eun

Kim1, Boram Choi1, Jeong Mi Seo1, Yeong-jin Lee1, June-Kyu

Park2, So-Jeong Cha2, Jinsoo Ko1, Gyeong In Lee1,2, Nakwon

Kwak3, Jae-Joon Yim3, and Jake Whang1*

1Research and Development Center, The Korean Institute of Tuberculosis,

Korean National Tuberculosis Association, 2Laboratory Medicine Center,

The Korean Institute of Tuberculosis, Korean National Tuberculosis

Association, 3Division of Pulmonary and Critical Care Medicine, Department

of Internal Medicine, Seoul National University Hospital

D034

Analysis of Antibiotic Resistance Patterns in

Gram-negative Multidrug-resistant Bacteria Using

Antibiotic Susceptibility Analysis

Joon Ki Kim, Hong Ki Kim, Byung Hak Kang, and Su Yeon Kim*

Division of Pathogen Resource Management, Center for Vaccine Research,

National Institute of Health, Korea Disease Control and Prevention Agency

(KDCA)

Poster

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D035

Biochemical and Molecular Characteristics of

Cryptococcus neoformans

Yewon An, SuYeon Kim, Byung Hak Kang, and Yun Ha Lee*

Division of Pathogen Resource Management, Center for Vaccine Research,

National Institute of Health, Korea Disease Control and Prevention Agency

(KDCA)

D036

Characterization of Carbapenem-resistant Klebsiella

pneumoniae Clinically Isolated in Korea

Hong Ki Kim, Joon Ki Kim, Byung Hak Kang, and Su Yeon Kim*

Division of Pathogen Resource Management, Center for Vaccine Research,

National Institute of Infectious Diseases, National Institute of Health, Korea

Disease Control and Prevention Agency (KDCA)

D037

Characteristic Analysis of Staphylococcus aureus

Registered in the National Culture Collection for

Pathogens (NCCP)

Ji Hee Lee, Yewon An, Byung Hak Kang, and Su Yeon Kim*

Division of Pathogen Resource Management, Center for Vaccine Research,

National Institute of Health, Korea Disease Control and Prevention Agency

(KDCA)

D038

Analysis of Antifungal Susceptibility in Aspergillus Species

of National Culture Collection for Pathogens (NCCP)

Yun Ha Lee, Yewon An, Su Yeon Kim, and Byung Hak Kang*

Division of Pathogen Resource Management, Center for Vaccine Research,

National Institute of Health, Korea Disease Control and Prevention Agency

(KDCA)

D039

Development and Validation of a Focus Reduction

Neutralization Test (FRNT) for Respiratory Syncytial

Viruses

Kirim Yoo, Sunkyung Yoon, Chae-yul Kim, June-Woo Lee, Hye

Sook Jeong, and WonSeok Gwak*

Division of Clinical Vaccine Research, Center for Vaccine Research National

Institute of Infectious Diseases, Korea National Institute of Health

D040

Clinical Presentation, Viral Shedding, and Neutralizing

Antibody Responses of Mpox Cases in South Korea:

Single Center Experience

Hyang su Kim1, Jihye Um1, Hye su Kim1, Hong soon Yim1, BumSik

Chin2, Min-Kyung Kim2, So Yun Lim3, Yeonjae Kim2, and Jun-Sun

Park1*

1Public Health Research Institute, National Medical Center, 2Division of

Infectious Diseases, Department of Internal Medicine, National Medical

Center, 3Division of Infectious Diseases, Department of Internal Medicine,

National Medical Center

D041

Characterization of LasB Inhibitor in Pseudomonas

aeruginosa

Cheol Seung Lee, Chae Ran Jeon, Xi-Hui Li, and Joon-Hee Lee*

Department of Pharmacy, College of Pharmacy, Pusan National University

D042

Development of Rapid Detection Methods with Single

Medium and Loop-mediated Isothermal Amplification

(LAMP) for Food-borne Pathogens in Ready-to-eat

(RTE) Foods

Ah Jeong Park, Jae hyun Ahn, Keu hoi Kim, and Jin young Beom*

Lotte R&D Center

D043

Optimization of the Cell-penetrating Peptides for ASFV

Multi-epitope Vaccines

Seonju Lee1 and Eun Bae Kim2*

1Department of Animal Industry Convergence, College of Animal Life

Science, Kangwon National University, 2Department of Applied Animal

Science, College of Animal Life Science, Kangwon National University

D044

Exploring the Microbial Community and Network

Structure of Cabbage Soft-rot Disease Suppressive Soil

Do-Hyun Kim

National Institute of Agricultural Sciences

D045

Fluoroquinolone-resistant Campylobacter Species

Isolated from Poultry Farms and Retail Chicken Meat in

Korea

Ji Hyun Lim, Jun Bong Lee, Ji Heon Park, and Soo-Jin Yang*

Department of Veterinary Microbiology, College of Veterinary Medicine and

Research Institute for Veterinary Science, Seoul National University

D046

Mechanistic Insights into the Enterohemorrhagic E. coli

Shiga Toxin 2a-Induced Renal Injury under Hypertonic

Stress

So-Hyeon park1,2, Kyung-Soo Lee2, Jun-Young Park2, Mirim Kim2,3,

Hae Ryeon Lee2,3, Subin Park2, Chang-Ung Kim2*, and Moo-Seung

Lee2,3*

1Department of Biochemistry, College of Natural Science, Chungnam National

University, 2Environmental Disease Research Center, Korea Research

Institute of Bioscience and Biotechnology, 3Biomolecular Science, Korea

University of Science and Technology (UST)

2024 International Meeting of the Microbiological Society of Korea

254 | www.msk.or.kr

D047

Transcriptomic Analysis on Escherichia coli in Response

to Endolysin LNT113 Treatment

Hyemin Song1, Heejoon Myung2,3, and Hyunjin Yoon1,4*

1Department of Molecular Science Technology, Ajou University, 2LyseNTech

Co., Ltd., 3Department of Bioscience and Biotechnology, Hankuk University

of Foreign Studies, 4Advanced College of Bio-convergence Engineering,

Ajou University

D048

Prevalence and Characteristics of Monophasic

Salmonella Typhimurium Associated with Different

Sources in Jeollanam-do

Eunbyeul Go, Bo Ra Kang, Hye Lin Yang, Eun Sun Kim, Hyo Jin Yu,

Mi Yeong Shin, and Sook Park*

Institute of Health and Environment of Jeollanam-do

D049

First Report of Sydowia polyspora Causing Current

Season Needle Necrosis (CSNN) on Abies koreana in

Korea

Jun-gi Lee

National institute of Forest Science

D050

Phage-antibiotic Treatment Strategies to Overcome

Phage Resistance in Phage Therapy for

Carbapenem-resistant Acinetobacter baumannii

Yoon-Jung Choi1, Shukho Kim1,2, Shamsuzzamn Md2, Md Rana

Shohel2, Minsang Shin1,2, and Jungmin Kim1,2*

1Department of Microbiology, School of Medicine, Kyungpook National

University, 2Department of Biomedical Sciences, The Graduate School,

Kyungpook National University

D051

Surveillance of the Distribution of Ticks Collected in

Gyeongsangnam-do, Korea

Jiyeon Um, Sein Bang, Suwan Choi, Sunhee Kim, Jangho Kim,

Hyeonjung Kim, Youngrok Kim, and Jihee Ahn*

Gyeongsangnam-do Provincial Government Health & Environment Institute

D052

Establishment of Sequence Analysis Methods for

Testing the Genetic Stability of Newcastle Disease

Vaccines

Geum Ji Seung and II Jang*

Animal and Plant Quarantine Agency

D053

Metabolic Reprogramming during Persistence Confers

Resuscitation from Dormancy in Bacteria during Infections

Jeong Moo Han1,2, Dongjoo Lee3, Jueun Lee4, Duhyun Ko1, Yeji

Bang1, Kwangsoo Kim5, Geum-Sook Hwang4, and Jinki Yeom1,2,5*

1Department of Microbiology and Immunology, College of Medicine, Seoul

National University, 2Institute for Data Innovation in Science, Seoul National

University, 3Interdisciplinary Program in Bioengineering, Seoul National

University, 4Integrated Metabolomics Research Group, Western Seoul

Center, Korea Basic Science Institute, 5Transdisciplinary Department of

Medicine and Advanced Technology, Seoul National University Hospital

D054

Subdomain of LasB Propeptide has Intracellular

Functions in Pseudomonas aeruginosa

Jae Yeon Gwak, Chae-Ran Jeon, Youngsun Shin, Fatma Ali Allam,

Xi-hui Li, and Joon-Hee Lee*

Department of Pharmacy, College of Pharmacy, Pusan National University

D055

Genetic Diversity and Relationship Analysis of V.

vulnificus Strains in Jeollanam-do, South Korea Using

MLST (Multilocus Sequence Typing)

Hae Young Na, Hyung Woo Lim, Sun Hee Lee, Go Eun Na, Eun Ji

Hwang, Yang Joon An, and Sook Park*

Institute of Health and Environment of Jeollanam-do

D056

Heterologous COVID-19 Vaccination Combining mRNA

and Adenoviral Vector Vaccines Elicits Robust Innate

and Adaptive Immune Responses

Ji Hyang Jeon1, Seongryong Kim2, Seo-Yeon Kim1, Yun-Ho

Hwang1, Tae-Young Lee1, Dokeun Kim1, Jong-Eun Park2, You-Jin

Kim1, and Jinah Yeo1*

1Division of Infectious Disease Vaccine Research, National Institute of

Health, Korea Disease Control and Prevention Agency, 2Graduate school of

Medical Science and Engineering, Korea Advanced Institute of Science and

Technology (KAIST)

D057

Molecular Epidemiology Study of Human-derived

Severe Fever with Thrombocytopenia Syndrome Virus

Cases in Gyeongsangnam-do

Minjae Kim, Gahee Bae, Yonkyoung Ha, Suwan Choi, Gyeonghwa

kim, Jangho Kim, Boram Kang, Sein Bang, Youngrok Kim, and

Jedong Kim*

Gyeongsangnam-do Provincial Government Health & Environment Institute

D058

Immunogenicity Evaluation of a Bivalent mRNA

Tuberculosis Vaccine

So Hee Park, Seojeong An, Mi-ran Yun, Soo-Min Kim, Jin-seung

Yun, You-Jin Kim, Dokeun Kim, and Eunkyung Shin*

Division of Infectious Disease Vaccine Research, National Institute of

Health, Korea Disease Control and Prevention Agency

Poster

www.msk.or.kr | 255

D059

Trends in Norovirus Distribution among Food Poisoning

Patients in Gyeongsangnam-do, Korea

Areum Jo, Daeun Lee, Yeon-ju Choi, So-young Kim, Jiyeon Um,

Min-young Kim, Jae-dong Kim, and Jihee Ahn*

Gyeongsangnam-do Provincial Government Health & Environment Institute

D060

IscR-Dependent Morphological Changes Protect

Acinetobacter baumannii from Oxidative Stress

Hoan Ngo1,2,3 and Jinki Yeom1,2,3,4*

1Department of Biomedical Science, College of Medicine, Seoul National

University, 2Department of Microbiology and Immunology, College of

Medicine, Seoul National University, 3Institute of Endemic Diseases, College

of Medicine, Seoul National University, 4Cancer Research Institute, Seoul

National University

D061

Selection of Candidate Antigens for Multi-epitope

Vaccines against Dirofilaria immitis

SungHyun Park and Eun Bae Kim*

Department of Applied Animal Science, College of Animal Life Science,

Kangwon National University

D062

Chlorella Virus as DNA Virus Surrogate for the Mass

Screening of Antiviral Substances

SuJin Jang, Su Yeon Kim, and Tae-Jin Choi*

Department of Microbiology and School of Marine and Fisheries Life Sciences,

Pukyong National University

D063

Reduction of Serine Protease-degrading Commensals

Exacerbates Colitis

Dongyeol Kim1, Nitsan Maharshak2, Yosep Ji3, Wilhelm

Holzapfel1,4, Dirk Haller4, Changkyun Lee5, and Hongsup Yoon1*

1Department of Life Science, Handong University, 2Department of Gasroenterology

and Liver Diseases, Tel Aviv Medical Center HEM pharma, 3Department of

Advanced Conversions, Handong University, 4Department of Nutrition and

Immunology, Technical University of Munich, 5Department of of Gastroenterology,

Kyung Hee University College of Medicine

D064

Structural Features and Roles of O-Glycans Attached to

the Cryptococcus neoformans Mannoproteins, CDA1

and MP88, in the Interaction with Host Immune Cells

Seonwoo Song1, Eun Jung Thak1,2, and Hyun Ah Kang1*

1Department of Life Science, Chung-Ang University, 2College of Pharmacy,

Dong-Duk Women’s University

D065

The Effect of pH on the Pseudomonas aeruginosa PA14

Biofilm

Taeung Moon, Yu-Bin Kim, Seo-Young Hong, Hae-Un Kim, and

Han-Shin Kim*

Division of Biotechnology, College of Environmental and Bioresource

Sciences, Jeonbuk National University

D066

Evaluation of the Inhibitory Effect of Multiwavelength

VCSEL Laser on Growth of Antifungal Drug-resistant

Dermatophytes

Seon Kang1, Eui-Seong Kim1, Soon-Yong So2, Jin Park3, and

Kyung-Tae Lee1*

1Korea Zoonosis Research Institute, Jeonbuk National University, 2Optowell

Co.,Ltd., 3Department of Dermatology, Jeonbuk National University Medical

School

D067

Comparative Genetic Analysis of Shiga Toxin-producing

Escherichia coli Isolates Collected in South Korea

Se Kye Kim and Jang Won Yoon*

College of Veterinary Medicine and Institute of Veterinary Science, Kangwon

National University

D068

Potential of PM-14 as an Adjuvant to Colistin for

Combating Antibiotic-resistant Gram-negative Pathogens

Jin-Gyeong Yu and Wooseong Kim*

College of Pharmacy, Ewha Womans University

D069

Antimicrobial Properties of PM16: Targeting MRSA and

Acinetobacter baumannii with Membrane Disruption

and Synergistic Effects

Jiwoo Choi and Wooseong Kim*

College of Pharmacy, Ewha Womans University

D070

Dual Function of BCB: Targeting Persister Cells in

Gram-positive Bacteria and Enhancing Colistin Efficacy

in Gram-negative Strains

Jin Kim and Wooseong Kim*

College of Pharmacy, Ewha Womans University

D071

RS-01 Exhibits Antimicrobial and Adjuvant Propensity

against MRSA Persisters

Seongeun Baek, Nakyung Lee, and Wooseong Kim*

College of Pharmacy, Ewha Womans University

2024 International Meeting of the Microbiological Society of Korea

256 | www.msk.or.kr

D072

Dysregulated Hematopoiesis Drives Hematological

Abnormalities in Severe Fever with Thrombocytopenia

Syndrome

Kyeongseok Jeon1,2, Hyo-Jin Ro1,2, Joowan Kim1,2, Yebeen Lee1,2,

Uni Park1,2,3, Yuri Kim1,2,3, Yoon Hwan Chang4, Yoon Kyung Jeon5,

Dong-Min Kim6, Yeon-Sook Kim7, and Nam-Hyuk Cho1,2,3,8*

1Department of Microbiology and Immunology, Seoul National University

College of Medicine, 2Department of Biomedical Sciences, Seoul National

University College of Medicine, 3Institute of Endemic Disease, Seoul

National University Medical Research Center, 4Department of Laboratory

Medicine, Seoul National University College of Medicine, 5Department of

Pathology, Seoul National University College of Medicine, 6Department of

Internal Medicine, Chosun University College of Medicine, 7Department of

Internal Medicine, Chungnam National University School of Medicine, 8Seoul

National University Bundang Hospital

D073

Tamoxifen Mitigates Primary Sclerosing Cholangitis

through Modulation of the Gut Microbiota

Ji Ho Yoo1, June-Young Lee2, In-Chul Jeong2, Su-Won Jeong2,

Jeong Eun Han2, Do-Yeon Kim2, and Jin-Woo Bae1,3*

1Department of Biomedical and Pharmaceutical Sciences, Kyung Hee

University, 2Department of Biology, Kyung Hee University, 3Department of

Biology, Kyung Hee University

D074

Comparative Metagenomic Analysis of Anti-PD1

Responders vs. Non-responders Identifies a Fecal

Microbial Consortium with Anti-tumor Activity in

Melanoma

Uk Jin Jeong1,2, Mohammed Ali1,3, Jin Sun You1,4, and Sang Sun

Yoon1,2,3,4,5*

1Department of Microbiology and Immunology, Yonsei University College of

Medicine, 2Brain Korea 21 Project for Medical Sciences, Yonsei University

College of Medicine, 3BioMe Inc., 4Institute for Immunology and Immunological

Diseases, Yonsei University College of Medicine, 5Severance Biomedical

Science Institute, Yonsei University College of Medicine

D075

Exploring the Potential of Spore-forming Bacteria for

Alleviating Inflammatory Bowel Disease (IBD)

Do Hun Gim1, So Yeon Lee2, and Jin-Woo Bae3*

1Department of Biology, Kyung Hee University, 2Department of Biology,

Kyung Hee University, 3Department of Biology and Department of Biomedical

and Pharmaceutical Sciences, Kyung Hee University

D076

Different Effects of Fiber Amount and Type on the

Amelioration of DSS-induced Colitis and Gut Microbiota

So-Yeon Lee and Jin-Woo Bae*

Department of Biology, Kyung Hee University

D077

Engineering Hybrid Endolysins to Treat Clostridioides

difficile Infection

Jieun An1, Siyoung Yoo1, Gowon Kim2, Kyuwon Shin2, Yein Jung3,

and Hyunjin Yoon1,4*

1Department of Molecular Science and Technology, Ajou University, 2Department

of Applied Chemistry and Biological Engineering, Ajou University, 3Department

of Biological Sciences, Ajou University, 4Advanced College of Bio-

convergence Engineering, Ajou University

D078

Skin Microbiome Analysis for Diagnosing Scabies and

Predicting the Onset of Post-scabies Eczema

Da Sol Kim1,2, Jungsoo Lee1,2,3, Kihyuk Shin1,2,3, and Hyun-Chang

Ko1,2,3*

1Department of Dermatology, Pusan National University Yangsan Hospital,

2Research Institute for Convergence of Biomedical Science and Technology,

Pusan National University Yangsan Hospital, 3Department of Dermatology,

College of Medicine, Pusan National University

D079

Characterization of Antimicrobial Actinomycetes

against Antibiotic-resistant Isolated from Livestock

Manure Compost

Dasom Kim and Song-Ih Han*

Department of Microbial Biotechnology, Mokwon University

D080

Nutrient Gradients Shape Colorectal Cancer

Pathogenesis by Regulating Toxin Expression in the Gut

Microbe

Eunmi Chu1,2, June-Yong Lee3, and Jinki Yeom1,2,4*

1Department of Biomedical Science, College of Medicine, Seoul National

University, 2Cancer Research Institute, Seoul National University, 3Department

of Microbiology and Immunology, Yonsei University College of Medicine,

4Department of Microbiology and Immunology, College of Medicine, Seoul

National University

D081

Prevalence and Characterization of Foodborne

Pathogens Isolated from Duck Carcass of

Slaughterhouses from 2019 to 2024 in Korea

Hye-Rin Lee, Jiyeon Jeong, Hye-Ri Jung, Jin-San Moon, Min-Su

Kang, Moon Her, and Myeongju Chae*

Avian Disease Research Division, Animal and Plant Quarantine Agency

D082

Penicillin-binding Protein Imbalance Drives OMV

Production in Salmonella Exposed to Non-lethal

Cephalexin

Jihye Yang, Yongjun Son, Yerim Park, and Woojun Park*

Laboratory of Molecular Environmental Microbiology, Department of

Environmental Sciences and Ecological Engineering, Korea University

Poster

www.msk.or.kr | 257

D083

Identification and Validation of Small Molecule Inhibitor

Targeting Biofilm Formation Regulator CsgD

Yunjeong Kim, Jihye Park, and Byoung Sik Kim*

Department of Food Science and Biotechnology, College of Engineering,

Ewha Womans University

D084

Selenium Nanoparticles Synthesized by Lactobacillus

plantarum as a Gram-positive Selective Bactericidal

Agent

Gyeongmin Kim, Sohyun Park, and Kwang-sun Kim*

Department of Chemistry, Pusan National University

2024 International Meeting of the Microbiological Society of Korea

258 | www.msk.or.kr

E001

Functional Distinctions of Escherichia coli K-12

Potassium Ion Transporters TrkG and TrkH

Ellen Tanudjaja1, Naomi Hoshi1, Kaneyoshi Yamamoto2, Kunio

Ihara3, Tadaomi Furuta4, Masaru Tsujii1, Yasuhiro Ishimaru1, and

Nobuyuki Uozumi1*

1Department of Biomolecular Engineering, Graduate School of Engineering,

Tohoku University, Sendai 980-8579, Japan, 2Department of Frontier

Bioscience, Hosei University, Tokyo 184-8582, Japan, 3Center for Gene

Research, Nagoya University, Nagoya 464-8602, Japan, 4School of Life

Science and Technology, Tokyo Institute of Technology, Yokohama

226-8501, Japan

E002

Bacterial Cytoplasmic Copper Stress Causes Amino Acid

Auxotrophy but is Dispensable in the Murine Infection

Model of Salmonella Typhimurium

Mina Kim and Iel Soo Bang*

Department of Microbiology and Immunology, Chosun University School of

Dentistry

E003

A Novel Flux Regulation of Lysine Acetyltransferase

(PatZ) in Escherichia coli

Gwanwoo Lee and Yeong-Jae Seok*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

E004

Identification of the Role of the Inducer-binding Motif

in the LysR-type Regulator NhaR in Escherichia coli

Yeasol Cho1, Eunsil Choi2, and Jihwan Hwang1,2*

1Department of Integrated Biological Sciences, Pusan National University,

2Microbiological Resource Research Institute, Pusan National University

E005

Hepatoprotective Effects of Lactic Acid Bacteria in

Ethanol-induced Liver Injury

Cho Hee Kim, Do Yi Kim, Chan Woo Jeon, Hyeon Yeong Lee, Hong

Sik Kim, and Jung Hoon Yoon*

Sungkyunkwan University

E006

Identification of Lytic Transglycosylases Necessary for

Intrinsic Antibiotic Resistance and Daughter Cell

Separation under Acidic pH Conditions in Escherichia

coli

Ji eun Son, Umji Choi, and Chang-Ro Lee*

Department of Biological Sciences, Myongji University

E007

Characterization of a Newly Discovered Lung

Bacterium Selenomonas sputigena and Its Implications

for Respiratory Health

Yukyung Kim1, Nguyen Thi Trinh1, Hyunki Shin1, Ye-Eun Ra1, and

Ye-Ji Bang1,2*

1Department of Biomedical Sciences, College of Medicine, Seoul National

University, 2Department of Microbiology and Immunology, College of

Medicine, Seoul National University

E008

The Effect of EIIANAG on FAK System Involved in

Exogenous Fatty Acid Incorporation in

Faecalibacterium prausnitzii A2-165

Dahyeon Son and Yeong Jae Seok*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

E009

Regulation of Salmonella Pathogenicity Island-1 (SPI-1)

Genes by RNase III in Salmonella Typhimurium

Donghwee Kim1, Seungmok Han1, Ji-Won Byun1, Kahyun Kim1, and

Minho Lee1,2*

1Department of Microbiology, College of Medicine, Hallym University,

2Institute of Medical Science, College of Medicine, Hallym University

E010

Utilizing the SplitFAST System to Discover Novel

Antibiotics Targeting Key Protein-protein Interactions

in Gram-negative Bacteria

Yurim Yoon, Minhee Oh, and Saemee Song*

Korea Research Institute of Chemical Technology

E011

Genetic Engineering of Auxotrophic Clostridium sp. AWRP

Ji-in Yang1,2, Soo Jae Kwon1,2, Hae Jun Kwon1, Joungmin Lee1,2,

and Hyun Sook Lee1,2*

1Marine Biotechnology & Bioresource Research Department, Korea Institute

of Ocean Science and Technology, 2KIOST School, University of Science and

Technology

E012

Adaptive Functions of Surrogate Membrane Lipids in

Pseudomonas aeruginosa during Phosphate Starvation

Huiyan Li, Alexandra winkler, and Joon-Hee Lee*

Department of Pharmacy, College of Pharmacy, Pusan National University

E013

Mapping of Cold Environment-specialized Protein

Homeostasis System in Pseudomonas antarctica

Seong-Hyeon Kim, HeeChan Koo, and Changhan Lee*

Department of Biological Sciences, Ajou University

Poster

www.msk.or.kr | 259

E014

Effects of Blue Light on Fruiting Body Production and

Ergothioneine Content during Sawdust Cultivation of

Shiitake (Lentinula edodes)

Min-Jun Kim, Ye-Yeong Kim, Aruna Jo, Mi-Jeong Park,

Kang-Hyeon Ka, and Yeongseon Jang*

Forest Microbiology and Application Division, Department of Forest Bio-

resources, National Institute of Forest Science

E015

Elucidating the Effects of HPr on ManR-regulated

Cell-cell Aggregation in Vibrio cholerae

Minchung Kang and Yeong-Jae Seok*

School of Biological Sciences and Institute of Microbiology, Seoul National

University

E016

Self-regulating and Aggregate Binding Mechanism of

AAA+ Disaggregating Chaperone ClpG in Pseudomonas

aeruginosa

Seongjoon Moon, Hyunhee Kim, and Changhan Lee*

Department of Biological Sciences, Ajou University

E017

Functional Studies of Ribosomal Protein S21 in B.

subtilis

Jiwoon Jung1, Yong Joon Kim1, Seo Hyun Park1, Joon Suk Lee1,

Chang Jun Ji2, and Yoon Mo Yang1*

1Graduate School of Biomedical Science and Engineering and Hanyang

Institute of Bioscience and Biotechnology, Hanyang University, 2Department

of Life Science and Research Institute for Natural Sciences, Hanyang

University

E018

The First Acid Chaperone in Pseudomonas aeruginosa

Activated through pH-Dependent Structural Changes

Soojeong Ham1, Yeon-Ju Jung2, Hyunhee Kim1, Sun-Shin Cha2*,

and Changhan Lee1*

1Department of Biological Sciences, Ajou University, 2Department of

Chemistry & Nanoscience, Ewha Womans University

E019

Characterization of Hsp100 Disaggregating

Chaperones in Pseudomonas putida

Sangha Kim, Seongjoon Moon, and Changhan Lee*

Department of Biological Science, Ajou University

E020

The Anti-aggregating Activity of HspB1 in the Naked

Mole Rat on Neurodegenerative Disease-associated

Proteins

Jeongyun Jeong, Juwon Jeong, Yoonchae Jeong, Hyunhee Kim*,

and Changhan Lee*

Department of Biological Sciences, Ajou University

E021

Identification Genes Involved in Glyoxal Resistance in

Escherichia coli

Jeonghwa Yu and Changhan Lee*

Department of Biological Sciences, Ajou university

E022

Genetic Change in Enterobacter cloacae Modifying

Pathogenicity under Microgravity

Hyochan Jang1, Alfredo Alcantara Jr.2, Atsushi Higashitani3, Jin I.

Lee2*, and Robert James Mitchell1*

1School of Life Sciences, Ulsan National Institute of Science and Technology

(UNIST), 2Division of Biological Science and Technology, College of Science

and Technology, Yonsei University, 3Graduate School of Life Sciences,

Tohoku University, Sendai 980-8577, Japan

E023

Study on Detoxification of Benzo[a]pyrene by Lactic

Acid Bacteria

Doyi Kim, Cho Hee Kim, Chan Woo Jeon, Hyeon Yeong Lee, Hong

Sik Kim, and Jung Hoon Yoon*

Department of Food Science and Biotechnology, Sungkyunkwan University

E024

Characterization of the Calcium-activated Potassium

Channel PotA in Cell Migration

Taeck Joong Jeon1,2 and Dahyeon Kim1,2*

1Department of Integrative Biological Sciences & BK21 FOUR Educational

Research Group for Age-associated Disorder Control Technology, Chosun

University, 2Research Institute of Chosun Natural Science & CSU G-LAMP

Project Group, Chosun University

E025

KCNMA1 Is Involved in Electrotaxis in Dictyostelium

Taeck Joong Jeon1,2 and Hyunjoong Kim1,2*

1Department of Integrative Biological Sciences & BK21 FOUR Educational

Research Group for Age-associated Disorder Control Technology, Chosun

University, 2Research Institute of Chosun Natural Science & CSU G-LAMP

Project Group, Chosun University

2024 International Meeting of the Microbiological Society of Korea

260 | www.msk.or.kr

E026

Cytoplasmic Calcium Responses upon External Calcium

Stimuli in Dictyostelium

Taeck Joong Jeon1,2 and Dahyeon Kim1,2*

1Department of Integrative Biological Sciences & BK21 FOUR Educational

Research Group for Age-associated Disorder Control Technology, Chosun

University, 2Research Institute of Chosun Natural Science & CSU G-LAMP

Project Group, Chosun University

E027

The Role of the Cytochrome bcc1-aa3 Supercomplex of

the Respiratory Electron Transport Chain in Modulating

SigF Functionality in Mycobacterium smegmatis

Yuna Oh1, Yunkyung Kim1, and Jeong-Il Oh1,2*

1Department of Integrated Biological Science, Pusan National University,

2Microbiological Resource Research Institute, Pusan National University

E028

The Regulatory Systems in Mycobacterium smegmatis

Activated by Depletion of Inorganic Phosphate:

SenX3-RegX3, SigE-SigB and SigF

Youjin Seung1 and Jeong-Il Oh1,2*

1Department of Integrated Biological Science, Pusan National University,

2Microbiological Resource Research Institute, Pusan National University

E029

Enhancement of Enzymatic Degradation and

Antioxidant Activity of Sericin Protein Using Marine

Microorganisms

Joo Young Yang1, Ji Yeong Park1, Sondor Ganbat1, Dariimaa

Ganbat1, Yun Jae Kim2, Bo Gyoung Choi2, GaeWon Nam3, Yo Han

Jang4, Mi-Hwa Park5*, and Sang-Jae Lee1*

1Department of Food Biotechnology, Research Center for Extremophiles &

Marine Microbiology, Silla University, 2Korea Institute of Ocean Science and

Technology, 3Department of Bio-cosmetics, Seowon University, 4Department

of Biological Sciences and Biotechnology Major in Bio-Vaccine Engineering,

Andong National University, 5Department of Food and Nutrition, College of

Medical and Life Science, Silla University

E030

The Mechanism Underlying the Decreased Resistance

of Mycobacterium smegmatis to Isoniazid by the

Inactivation of cAMP Receptor Protein

Narin Kim1 and Jeong-Il Oh1,2*

1Department of Integrated Biological Science, Pusan National University,

2Microbiological Resource Research Institute, Pusan National University

E031

Effect of Microbial Fermentation on Antioxidant,

Antidiabetic, and Anti-osteoporotic Activities of

Schizophyllum commune and Irpex lacteus

Ji Yeong Park1, Joo Young Yang1, Sondor Ganbat1, Min Kyeong

Kim2, Won-Jae Chi2, Yong-Jik Lee3, Seok-Cheol Cho4, Myong Ki

Kim4, Sang-Jae Lee1*, and Mi-Hwa Park5*

1Department of Food Biotechnology, Research Center for Extremophiles &

Marine Microbiology, Silla University, 2Species Diversity Research Division,

National Institute of Biological Resources, 3Department of Bio-cosmetics,

Seowon University, 4Department of Food Science & Engineering, Seowon

University, 5Department of Food and Nutrition, College of Medical and Life

Science, Silla University

E032

Development of Ex Vivo Screening Tool to Discover

Gut-microbiome Factors Associated with the Protein

Stability of ɑ-Synuclein

Juwon Jeong and Changhan Lee*

Ajou University

E033

Fermentation of Horseradish Extract with Bacillus sp.

and Lactic Acid Bacteria (LAB) Results in Enhanced

Antioxidant Activities

Ji Yeong Park1, Joo Young Yang1, Sondor Ganbat1, Sang Geun

Park2, Young Im Choi2, GaeWon Nam3, Seok-Cheol Cho4, Yo Han

Jang5, Mi-Hwa Park6*, and Sang-Jae Lee1*

1Department of Food Biotechnology, Research Center for Extremophiles &

Marine Microbiology, Silla University, 2DAERYEOK INCORPORATED, 3Department

of Cosmetic Science & Technology Seowon University, Seowon Global Skin

Research Center, 4Department of Food Science & Engineering, Seowon

University, 5Department of Biological Sciences and Biotechnology Major in

Bio-Vaccine Engineering, Andong National University, 6Department of Food

and Nutrition, College of Medical and Life Science, Silla University

E034

Catalytic Relevance of Substrate Configurations:

Structural Perspectives on ACC-1

Hyun-Ju Kim1, Da-Woon Bae1, Ye-Eun Jung1, Young Jun An2,

Jung-Hyun Na1, and Sun-Shin Cha1*

1Ewha Womans University, 2Korea Institute of Ocean Science and Technology

(KIOST)

E035

Role of a GlcNAc-sensing MCP in Chemotactic Response

of Vibrio vulnificus to the Intestinal Mucus

So-Min Cho, Bo-Ram Jang, Young-Jin Song, Sebin Kang, and

Kyu-Ho Lee*

Sogang University

Poster

www.msk.or.kr | 261

E036

New Insights into Carbapenem Inhibition of ACC-1 Class

C β-Lactamase

Se-Been Kim, Da-Woon Bae, Ye-Eun Jung, Bo-Gyeong Jeong, and

Sun-Shin Cha*

Ewha Womans University

E037

Physiology of Desemzia sp. Strain C1 Leading to High

H2O2 Production

Yongseok Ko1, Ha Pham2, Sunil Ghatge1, Yong-Hak Kim2, and

Hor-Gil Hur1*

1School of Earth Sciences and Environmental Engineering, Gwangju Institute

of Science and Technology (GIST), 2Department of Microbiology, Daegu

Catholic University School of Medicine

2024 International Meeting of the Microbiological Society of Korea

262 | www.msk.or.kr

F001

Machine-learning Analysis of Streptomyces coelicolor

Transcriptomes Reveals a Transcription Regulatory

Network Encompassing Biosynthetic Gene Clusters

Yongjae Lee1,2, Donghui Choe3, Bernhard O. Palsson3,4, and

Byung-Kwan Cho1,2,5*

1Department of Biological Sciences, Korea Advanced Institute of Science

and Technology, 2KI for the BioCentury, Korea Advanced Institute of

Science and Technology, 3Department of Bioengineering, University of

California San Diego, La Jolla, CA 92093, USA, 4Novo Nordisk Foundation

Center for Biosustainability, Technical University of Denmark, Kemitorvet,

Kongens, Lyngby, Denmark, 5Graduate School of Engineering Biology, Korea

Advanced Institute of Science and Technology

F002

Co-carriage of blaNDM-1 and blaVIM-2 in Different Plasmids

of Acinetobacter junii Isolate and Transfer of

blaNDM-1-Carrying Plasmids to Gram-negative Bacteria

Rana Md Shohel1, Shukho Kim1,2, Seo Yeon Ko1, Nayeong Kim1,

Seong Yeob Kim1, Da Eun Lee3, Ki Tae Kwon2,3,4, Yu Kyung Kim2,3,5,

and Je Chul Lee1,2,3*

1Department of Microbiology, School of Medicine, Kyungpook National

University, 2Untreatable Infectious Disease Institute, Kyungpook National

University, 3Kyungpook National University Hospital National Culture

Collection for Pathogens (KNUH-NCCP), Kyungpook National University

Hospital, 4Department of Internal Medicine, School of Medicine, Kyungpook

National University, 5Department of Laboratory Medicine, School of

Medicine, Kyungpook National University

F003

AmiC Modulates RNase E-Mediated Decay of SgrS RNA

in Response to Glucose Phosphate Stress in Escherichia

coli

Yubin Yang1, Jungyeop Kim1, Hana Hyeon1, Yuhyun Jang1, Minju

Joo1, Eunkyoung Shin2, and Kangseok Lee1*

1Department of Life Science, Chung-Ang University, 2Department of Microbiology,

Catholic University of Daegu School of Medicine

F004

Inhibition of a Detrimental Gene by the Cas9-sgRNA

Ribonucleoprotein Complex in Levilactobacillus brevis

Min Young Kwon, So Young Park, Hea Joon Kim, and Hyo Jin Kim*

Graduate School of International Agricultural Technology, Seoul National

University

F005

In Silico Genome Analysis of Various Adeno-associated

Virus Serotypes

Jihong Min1,2, Chanhee Lee1,2, Anyeseu Park1,2, and Jeong Yoon

Lee1,2*

1The Laboratory of Viromics and Evolution, Korea Zoonosis Research

Institute, Jeonbuk National University, 2Department of Veterinary and

Animal Science, Jeonbuk National University

F006

Analysis of Genetic Diversity of Tricholoma matsutake

in Hongcheon, Republic of Korea Using SSR Markers

Min-Jun Kim, Ye-Yeong Kim, Aruna Jo, Yeongseon Jang,

Kang-Hyeon Ka, and Mi-Jeong Park*

Forest Microbiology and Application Division, Department of Forest Bio-

resources, National Institute of Forest Science

F007

Complete Genome Sequence of Formate-utilizing

Bacteria, Cupriavidus sp. WKF15(NIBRBAC000509970),

and Ancylobacter sp. WKF20 (NIBRBAC000510447)

Hae Soo Shin1, Hye Yoon Park1, Jong Seok Lee2, Yoonyong Yang2,

Soonok Kim1*, and Jong-Geol Kim3*

1Biological Resources Utilization Division, National Institute of Biological

Resources, 2Biological Resources Assessment Division, National Institute of

Biological Resources, 3Department of Biological Science, Wonkwang University

F008

Application of DNA Metabarcoding for the Detection of

Biological Ingredients in Lactobacillus Products

Min Jeong Kim, Han Chul Lee, and Sung Min Kim*

Forensic Genetics & Chemistry Division, Supreme Prosecutors’ Office

F009

Simple Sequence Analysis of the S1 Gene in IBV Vaccine

Strains Using Next-generation Sequencing

Tae Gyun Kim and Il Jang*

Veterinary Drugs and Biologics Division, Animal and Plant Quarantine

Agency

F010

Complete Genome Sequence of a

Rhizosphere-associated Bacterium with Potential for

Plant Growth Promotion

Yujin Hyun1, Min-Ji Kim2, and Jae-Ho Shin1,2,3*

1Department of Integrative Biology, Kyungpook National University, 2Department

of Applied Biosciences, Kyungpook National University, 3NGS Core Facility,

Kyungpook National University

F011

Unraveling the Transcriptional and Kinase Network

Mechanisms Underlying Radiation Resistance in

Cryptococcus neoformans

Kwang-Woo Jung1, Sunhak Kwon1,2, Jong-Hyun Jung1, Sangyong

Lim1,3, Yong-Sun Bahn2, and Kwang-Woo Jung1*

1Advance Radiation Technology Institute, Korea Atomic Energy Research

Institute, 2Department of Biotechnology, College of Life Science and

Biotechnology, Yonsei University, 3Department of Radiation Science,

University of Science and Technology

Poster

www.msk.or.kr | 263

F012

Next Generation Sequencing (NGS) Analysis of Lesions

from Mpox-infected Patients

Hongsoon Yim1, Jihye Um1, Hyang su Kim1, BumSik Chin2,

Min-Kyung Kim2, So Yun Lim2, Yeonjae Kim2, and Jun-sun Park1*

1Public Health Research Institute, National Medical Center, 2Division of

Infectious Diseases, Department of Internal Medicine, National Medical Center

F013

Challenges in Assessing Genetic Stability of IBD Vaccines

with Phylogenetic Insights from the Hypervariable

Region of the VP2 Gene

Seona Jo and Il Jang*

Veterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency

F014

Genus Deinococcus Species as a Model Organisms of

Anti-oxidation Responses and Their Transposition of

Insertion Sequence Elements

Yujin Park1,2, Seongik Kim1, Minje Sung1, Hyeseong Hwang1, Minji

Kim1, and Sung-Jae Lee1,2*

1Department of Biology, 2Korea Institute of Ornithology, Kyung Hee University

F015

Probiotics Genetic Engineering for Expression of CBD

and CVD-associated Potential Therapeutic Agents

So Jin Lee1, Jun Hyeok Yang1, Hye-Ji Kim1, Da Young Lee1, Ki Na

Yun1, Heui Kyoung Cho1, Hyun Bong Park2, Harry Jung3, Jin

Pyeong Jeon4, Jun Yup Kim5, Si Un Lee6, Hyo-Jung Lee7, Chang

Wan Oh6, and Jong Kook Moon1*

1BTSynergy Co., Ltd., 2Department of Biology, College of Natural Sciences,

Gangneung-Wonju National University, 3Institute of New Frontier Research,

Hallym University College of Medicine, 4Department of Neurosurgery,

Hallym University College of Medicine, 5Department of Neurology, Seoul

National University Bundang Hospital, Seoul National University College of

Medicine, 6Department of Neurosurgery, Seoul National University Bundang

Hospital, Seoul National University College of Medicine, 7Department of

Periodontology, Section of Dentistry, Seoul National University Bundang

Hospital

F016

Genetic Diversity and Structure Analysis of Tricholoma

matsutake Mushrooms in Korea Using SSR Markers

Aruna Jo, Min-Jun Kim, Ye-Yeong Kim, Mi-Jeong Park, Yeongseon

Jang, and Kang-Hyeon Ka*

Forest Microbiology and Application Division, Department of Forest Bio-

Resources, National Institute of Forest Science

F017

The Condensin-mediated Chromosomal Organization in

Response to Iron Deficiency

Seong Ho An and Kyoung-Dong Kim*

Department of Systems Biotechnology, Chung Ang university

F018

Regulatory Interplay between Fep1 and Pho7: Insights

into Iron Acquisition and Phosphate Signaling Through

ChIP-seq

Seon-Ju Park, Seong Ho An, and Kyoung-Dong Kim*

Department of Systems Biotechnology, Chung-Ang university

F019

Distinct Role of Med8 in Regulating Ace2 Target Genes

in Schizosaccharomyces pombe

Ji Hyun Kim and Kyoung-Dong Kim*

Department of Systems Biotechnology, Chung-Ang University

F020

Skin Bacteria Affect Chromosome Architecture beyond

the Kingdom

Jae Min Lee and Kyoung-Dong Kim*

Department of Systems biotechnology, Chung-Ang University

F021

Whole-genome Sequencing Reveals Co-occurrence of

Tetracycline, Metal, and Biocide Resistance Genes in

Listeria monocytogenes Isolated from Raw Meat

Yoojung Hwang1,2, Jeong-Ih Shin1,3, Mi-Ran Seo4, Yeun-Jun

Chung1,3,5, and Seung-Hyun Jung1,2,3*

1Department of Medical Sciences, College of Medicine, The Catholic University

of Korea, 2Department of Biochemistry, The Catholic University of Korea,

3Integrated Research Center for Genome Polymorphism, Precision Medicine

Research Center, The Catholic University of Korea, 4ConnectaGen, 5Department

of Microbiology, The Catholic University of Korea

F022

Engineering of the Type IV Secretion System (T4SS) to

Inhibit the Growth of Multi Drug-resistant Bacteria

Juyoung Oh, Juhyun Kang, and Juhyun Kim*

School of Life Science and Biotechnology, Kyungpook National University

F023

Genomic Diversity in Bacterial Nanocellulose

Biosynthesis among Komagataeibacter and Closely

Related Genera

Hee-Jin Han1, Eun Ha Lee2, Young Hoon Jung3, Yue Jai Kang4,

Ju-Hyeong Jung5, Jaeyoung Choi6*, and Sung-Chul Hong1*

1Department of Food Science and Biotechnology, Kunsan National University,

2Smart Farm Research Center, Korea Institute of Science and Technology

(KIST), 3School of Food Science and Biotechnology, Kyungpook National

University, 4Department of Aquatic Life Medicine, Kunsan National University,

5Department of Environmental Engineering, Kunsan National University,

6Deparment of Oriental Medicine Biotechnology, Kyung Hee University

2024 International Meeting of the Microbiological Society of Korea

264 | www.msk.or.kr

F024

The Myb-like Protein MylA Regulates Fungal

Development, Aflatoxin Production and Pathogenicity

in Aspergillus flavus

He-Jin Cho1 and Hee-Soo Park1,2*

1School of Food Science and Biotechnology, Kyungpook National University,

2Department of Integrative Biology, Kyungpook National University

F025

Ortholog Analysis and Transformation of Glycoside

Hydrolase Genes in Hyperthermophilic Archaeon

Thermococcus Species

Jun Won Lee1, Jae Kyu Lim2, Hyun Sook Lee1, Sung Gyun Kang1,

Jung-Hyun Lee1, Kae Kyoung Kwon1, and Yun Jae Kim1*

1Marine Biotechnology Research Center, Korea Institute of Ocean Science

and Technology, 2Jeju Bio Research Center, Korea Institute of Ocean

Science and Technology

F026

Genomic Analysis of Antifungal Drug-associated

Candida auris

Jiwon Bang, Juhee Kim, Minjeong Kwon, and Yong-joon Cho*

Department of Integrative Molecular and Biomedical Sciences, College of

Biomedical Sciences, Kangwon National University

F027

Genomic Analysis of Cave-derived Streptomyces

Minjeong Kwon, Juhee Kim, Jiwon Bang, and Yong-Joon Cho*

Department of Integrative Molecular and Biomedical Sciences, College of

Biomedical Sciences, Kangwon National University

F028

Hfq Expression Level Modulation-coordinated

sRNA-based Gene Knockdown in Escherichia coli

Sangmyeong Bae, Yu Jung Jung, Keun Ha Park, Taeyoung Jang,

and Seung Min Yoo*

School of Integrative Engineering, Chung-Ang University

F029

Genomic and Virulence Profiling of Clinical and

Environmental Vibrio vulnificus Isolates

Jinkyeong Lee1,2, Jiyon Chu1,2, Mi-Ran Seo3, Yeun-Jun Chung1,4,5,

and Seung-Hyun Jung1,2,5*

1Department of Medical Sciences, College of Medicine, The Catholic

University of Korea, 2Department of Biochemistry, The Catholic University

of Korea, 3ConnectaGen, 4Department of Microbiology, The Catholic

University of Korea, 5Integrated Research Center for Genome Polymorphism,

Precision Medicine Research Center, The Catholic University of Korea

F030

Type IV Pilin Variable Regions That Determine the Host

Range of the RNA Phages in Pseudomonas aeruginosa

Hyeong-Jun Ki, Hee-Won Bae, Shin-Yae Choi, and You-Hee Cho*

Program of Biopharmaceutical Science and Department of Pharmacy, College

of Pharmacy and Institute of Pharmaceutical Sciences, CHA University

F031

CRISPR-guided Identification of Phage Elements Required

for Phage-host Interactions in Pseudomonas aeruginosa

Min-Ju Kim, Shin-Yae Choi, Su-Yeon Kim, Hee-Won Bae, and

You-Hee Cho*

Program of Biopharmaceutical Science and Department of Pharmacy,

College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University

F032

Dissemination of Antibiotic Resistance Genes in

Pseudomonas aeruginosa : Prioritizing Critical Factors

Nam-Hoon Kim1, Jung Hun Lee2, Dae-Wi Kim1, and Sang Hee Lee2*

1Department of Life Sciences, Jeonbuk National University, 2National Leading

Research Laboratory of Drug Resistance Proteomics, Department of Biological

Sciences, Myongji University

F033

Investigation of Pivotal Chemical Moieties of

Metallo-β-lactamase Inhibitors and Prioritization of

Target Metallo-β-lactamases for Inhibitor Design

Sang-Gyu Kim1, Jung Hun Lee2, Sang Hee Lee2, and Dae-Wi Kim1*

1Division of Life Sciences, Jeonbuk National University, 2A National Leading

Research Laboratory of Drug Resistance Proteomics, Department of

Biological Sciences, Myongji University

F034

Genome Analysis of Bacillus licheniformis SK1230

Isolated from Pickled Pepper

JuHyun Song1, Sung-Ho Yoon2, Hyeon-Ji Kim2, Ah-Ran Lee3, and

Soo-Ki Kim1*

1Department of Animal Science, Konkuk University, 2Department of Bioscience

and Biotechnology, Konkuk University, 3Animal Resources Research Center,

Konkuk University

F035

Antibiotic Resistance Prediction of Mycobacterium

tuberculosis by Whole-genome Sequencing of Clinical

Isolates in South Korea

Jeong-Ih Shin1,2, In Young Yoo3, Jinkyung Lee1,4, Yoojung

Hwang1,4, Kyu-Hwa Hur5, Yeon-Joon Park3, and Seung-Hyun

Jung1,2,4*

1Department of Medical Sciences, College of Medicine, The Catholic

University of Korea, 2Integrated Research Center for Genomic Polymorphism,

Precision Medicine Research Center, College of Medicine, The Catholic

University of Korea, 3Department of Laboratory Medicine, Seoul St. Mary's

Hospital, College of Medicine, The Catholic University of Korea, 4Department

of Biochemistry, The Catholic University of Korea, 5Department of Laboratory

Medicine, Green Cross Laboratories

Poster

www.msk.or.kr | 265

F036

Whole Genome Sequencing and Metabolism Profiling

Reveal Genetic Structure and Growth-associated Genes

in Campylobacter jejuni

Jeong-Ih Shin1,2, Jiyon Chu1,3, Mi-Ran Seo4, Yeun-Jun Chung1,2,5,

and Seung-Hyun Jung1,2,3*

1Department of Medical Sciences, College of Medicine, The Catholic

University of Korea, 2Integrated Research Center for Genome Polymorphism,

Precision Medicine Research Center, The Catholic University of Korea,

3Department of Biochemistry, The Catholic University of Korea, 4ConnectaGen,

5Department of Microbiology, The Catholic University of Korea

F037

Genotypic Variations Required for Helicobacter pylori

to Infect and Adapt to a New Host

Jae Yun Nam1, DoWon Seo1, Sacheera Angulmaduwa1, Myeong-A

Kim2, Ashansa Ramanayake1, Min-Seo Kim1, Roshan Pradeep1,

SungKyung Lee1, Eui-Jeong Noh2, and Jeong-Heon Cha1,2*

1Yonsei University, The Graduate School, Department of Applied Life

Sciences, 2Yonsei University, College of Dentistry, Department of Oral

Biology

F038

Investigation of Heterogeneity of Helicobacter pylori

PMSS1 for Mouse Infectivity

Dowon Seo1, Jaeyun Nam1, Minseo Kim1, Sacheera

Angulmaduwa1, Ashansa Ramanayake1, Roshan Pradeep1,

Seongkyeong Lee1, Eui-jeong Noh2, Myeong-A Kim2, and

Jeong-Heon Cha1,2*

1Yonsei University, The Graduate School, Department of Applied Life Sciences,

2Yonsei University, College of Dentistry, Department of Oral Biology

F039

Roles of Helicobacter pylori fur Genotype Variants In

Vivo Infection

Min-Seo Kim1,2, Jae Yun Nam1,2, Do Won Seo1,2, Sacheera

Angulmaduwa1,2, Ashansa Ramanayake1,2, Roshan Pradeep1,2,

Sung-Kyong Lee1,2, Eui-Jeong Noh1,2, Myeong-A Kim1,2, and

Jeong-Heon Cha1,2*

1Department of Applied Life Sciences, Yonsei University, The Graduate

School, 2Department of Oral Biology, the Graduate School, BK21 FOUR

Project, Yonsei University College of Dentistry

F040

Whole Genome Sequencing of Vancomycin-resistant

Enterococcus faecium Strain Isolated from Dogs in

South Korea

Ali Md Sekendar1, Ji-Hyun Choi1, Dong-Chan Moon2, Jae-Myung

Kim1, Suk-Kyung Lim1*, and Bo-Youn Moon1*

1Animal and Plant Quarantine Agency, 2Korea Centers for Disease Control

and Prevention

F041

Aspergillus fumigatus HLH Transcription Factor is

Required for Conidiation and Early Asexual

Development

Kap-Hoon Han, Sang-Cheol Jun, Kun Wang, Zeshi Ma, and

Mohammed Abdo Elgabbar*

Department of Pharmaceutical Engineering, Woosuk University

2024 International Meeting of the Microbiological Society of Korea

266 | www.msk.or.kr

G001

In Silico Analysis of the Central Carbon Metabolism of

Selenomonas sputigena ATCC 35185 and

Understanding of Its Physiological Functions in

Promoting Lung Health

Trinh Nguyen Thi1, Yukyung Kim1, and Ye-Ji Bang1,2*

1Seoul National University College of Medicine Department of Microbiology

and Immunology, 2Seoul National University College of Medicine Department

of Biomedical Sciences

G002

In Vitro and In Silico Approaches for Predicting the

Hazards of Airborne Fungi in Indoor Environments

Hyewon Jeong1, Sung Yoon Ahn2, Sang Woong Lee2, and Iel Soo

Bang1*

1Department of Microbiology and Immunology, Chosun University School of

Dentistry, 2Pattern Recognition and Machine Learning Lab, Department of

AI Software, Gachon University

G003

Enhancement of Collectral Activity Using Engineered

CRISPR-Cas12j and Tailored Reporters

Younghoon Lee1,2, Seong Bo Hwang3, Kwang Hyun Park1, and Eu

Jeon Woo1*

1Critical Diseases Diagnostics Convergence Research Center, Korea Research

Institute of Bioscience and Biotechnology (KRIBB), 2Department of Proteome

Structural Biology, University of Science and Technology (UST), 3Bionanotechnology

Research Center, KRIBB

G004

Engineering an Omega Rhodopsin for Improved Lithium

Ion Selectivity through Directed Evolution

Kitae Kim1,2, Soon-Kyeong Kwon1*, and Jihyun Kim2*

1Research Institute of Molecular Alchemy, Gyeongsang National University,

2Department of Systems Biology, Division of Life Sciences, and Institute for

Life Science and Biotechnology, Yonsei University

G005

Enhancing Hydrogen (H2) Production Through

Integrated Evolutionary and Process Engineering of the

Hyperthermophilic Archaeon Thermococcus

onnurineus NA1

Sung-Mok Lee1, Hae-Chang Jung1,2, Bo Gyoung Choi1, Hyejin

Park1, Seong Hyuk Lee1, Hyun Sook Lee1,2, and Sung Gyun Kang1,2*

1Marine Biotechnology & Bioresource Research Department, Korea Institute

of Ocean Science and Technology, 2KIOST School, University of Science and

Technology

G006

Optimization of mRNA Structure for the Enhancement

of Stability and Translational Activity

Eunbin Kim, Yuna Hwang, and Hyukjin Lee*

Ewha Womans University

G007

Development of Immune Modulating Ionizable Lipid

Nanoparticle with Enhanced Neutralizing Ability

Yeji Lee1, Seongryong Kim2, Jong-Eun Park2, Jinah Yeo3, and

Hyukjin Lee1*

1Ewha Womans University, 2Korea Advanced Institute of Science and

Technology, 3Korean National Institute of Health, Korea Disease Control and

Prevention Agency

G008

Genomic Analysis of Weissella cibaria Isolated from

Nakji-jeotgal: Probiotic Potential and Stress Resistance

Haram Kim1, Gi Beom Keum1, Hyunok Doo1, Jinok Kwak1, Sumin

Ryu1, Yejin Choi1, Juyoun Kang1, Yeongjae Chae1, Sheena Kim1,

Hyeun Bum Kim1, Jinho Lee2, Jeongyun Kim3, and Hyeun Bum

Kim1*

1Department of Animal Biotechnology, Dankook University, 2Research

Institute of Natural Science and Department of Physics Education, Gyeongsang

Natiotnal University, 3Department of Physics, Dankook University

G009

Enhancing mRNA Stability through Chemical

Modification of Poly A Tail

Yuna Hwang, Eunbin Kim, and Hyukjin Lee*

Ewha Womans University

G010

The Introduction of Ligand for Enhanced Antisense

Oligonucleotide Delivery

Dahye Lee, Hyomin Kim, Yelim Choi, and Hyukjin Lee*

College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha

Womans University

G011

Robogut: An Advanced In Vitro Model for Evaluating

the Efficacy of LBP against Carbapenem-resistant

Enterobacteriaceae

Sumin Choi1, Sinseong Park1, Wonsik Mun1, Hyochan Jang1, Hyeon

seop Lee1,2, and Robert J. Mitchell1*

1School of Life Sciences, Department of Biological Sciences, Ulsan National

Institute of Science and Technology, 2Korea Institute for Ceramic

Engineering and Technology (KICET)

G012

Potential Soluble Expression of Human Interleukin-10

in Corynebacterium glutamicum

Kyoung-Kuk Been1, Ji-Yeon Oh1, Hae-Eun Yoon2, and Geun-Joong

Kim1*

1Department of Biological Sciences, Chonnam National University, 2Department

of Wood Science & Engineering, Chonnam National University

Poster

www.msk.or.kr | 267

G013

mRNA Based Protein Replacement Therapy for Genetic

and Chronic Diseases

Yujeong Jeong, Miyoung Oh, Hyein Jung, and Hyukjin Lee*

College of Pharmacy, Ewha Womans University

G014

Development of Lipid Nanoparticle Formulation for

Plasmid Delivery to Immune Cells

Yelim Choi, Hyo-Min Kim, and Hyukjin Lee*

College of Pharmacy, Ewha Womans University

G015

Genome-scale Metabolic Modeling to Develop Synbiotics

to Mitigate Methane Emission from Ruminants with

Potential Probiotics

Tae Young Kim1,2, Byeong Cheol Ban1, Jong Sik Jeong3, Ryuk Seok

Kang3, Tan Sol Park3, and Myung-Hoo Kim1,2*

1Department of Animal Science, College of Natural Resources & Life

Science, Pusan National University, 2Future Earth Research Institute, PNU

JYS Science Academy, Pusan National University, 3Department of Animal

Science and Technology, Chung-Ang University

G016

Construction of a Novel Baculovirus Overexpression

Vectors for Mass Production of RSV F Protein

Kirim Yoo, Sunkyung Yoon, Chae-yul Kim, June-Woo Lee, Hye

Sook Jeong, and WonSeok Gwak*

Division of Clinical Vaccine Research, Center for Vaccine Research National

Institute of Infectious Diseases, Korea National Institute of Health

G017

Metabolic Network Reconstruction and Phenome

Exploration of Solvent-tolerant Pseudomonas putida S12

Dohyeon Kim, Sol Han, Youngshin Kim, and Sung Ho Yoon*

Department of Bioscience and Biotechnology, Konkuk University

G018

Optimization of Zeaxanthin Production by Lutimonas

zeaxanthinifaciens YSD2104T Using Response Surface

Methodology

Yoon-Hee Jung1,2, Hyun-Ju Hwang1, Tae Wan Kim2, and Seung

Seob Bae1*

1National Marine Biodiversity Institute of Korea, 2Department of Biotechnology

and Bioengineering, Chonnam National University

G019

Development of mRNA-based Genome Editing Using

Adenine Base Editor

Youngyoon Yoon and Hyukjin Lee*

Ewha Womans University

G020

Quality Characteristics of Jinan-gun Highland Blueberry

Vinegar Using Acetobacter pasteurianus IJRG 10007

Jong Hee Yoo, Da Eun Lee, Da Eun Jeong, Tae Young Kim, and Hyo

Bin Oh*

Institute of Jinan Red Ginseng

G021

Bacteriophage-based Vectors for Targeted Gene Delivery

to Cancer Cells

Takashi Sugano, Srivani Veeranarayanan, Yoshifumi Aiba,

Kazuhiko Miyanaga, XinEe Tan, Kanate Thitiananpakorn, Shinya

Watanabe, and Longzhu Cui*

Division of Bacteriology, Department of Infection and Immunity, School of

Medicine, Jichi Medical University, Tochigi, Japan

G022

Discovery, Elucidation and Characterization of Novel

CRISPR-Cas Systems from Diverse Bacterial Genomes

Jaincy N Jayanand Sang-Seob Lee *

Department of Integrative Biotechnology, Sungkyunkwan University, Natural

Science Campus

G023

Fermentation Quality Characteristics of Beneficial

Bacteria Isolated from Meju (Fermented Bean Block)

Containing White and Red Ginseng of the Jinan Plateau

Da Eun Jeong, Da Eun Lee, Jong Hee Yoo, Tae Young Kim, and Hyo

Bin Oh*

Institute of Jinan Red Ginseng

G024

Evolution of YqhD Aldehyde Reductase with Superior

Enzymatic Activity Towards Various Aldehyde Compounds

Yoon Chae Jeong1 and Changhan Lee2*

1Ajou Energy Science Research Center, Ajou University, 2Department of

Biological Sciences, Ajou University

G025

Designing and Structure Validation of Recombined

Lytic Enzymes against Methanogens

Hyeonjun Lee and Eun Bae Kim*

Department of Applied Animal Science, College of Animal Life Science, Kangwon

National University

2024 International Meeting of the Microbiological Society of Korea

268 | www.msk.or.kr

G026

Isolation of Yeasts from Wetland in Chuncheon

ChanHaeng Lee1, Myeong-Gi Cha2, Seo-Hyeon Nam2, Seung-Jun

Kim2, Jung-Ah Yoon3, and Myoung-Dong Kim2,4*

1Department of Food Biotechnology, Kangwon National University, 2Department

of Food Science and Biotechnology, Kangwon National University, 3Department

of Food Biotechnology and Environmental Science, Kangwon National

University, 4Institute of Fermentation and Brewing, Kangwon National

University

G027

Novel CRISPR Interference Screening Method for

Enhanced Microbial Violacein Production

Song Hee Jeong1, Hyun Ju Kim2, and Sang Jun Lee1*

1Department of Systems Biotechnology, Chung-Ang University, 2Biological

Resources Research Department, Nakdonggang National Institute of

Biological Resources

G028

Identification of Putative Novel CRISPR Type I-C

Cas3-cascade Orthologue in Novel Marine Bacterium,

Nioella lutimaris sp. nov

Minseok Kang1, Jae-yon Yu2, Jaincy N Jayan1, and Sang-Seob

Lee1*

1Department of Integrative Biotechnology, College of Biotechnology and

Bioengineering, Sungkyunkwan University, 2Korean Environmental Microorganism

Resources Center, Institute of Biotechnology and Bioengineering, Sungkyunkwan

University

G029

Serially Linked Small RNAs-Mediated Multi-target Gene

Regulation for Efficient Metabolite Production

Su Min Oh, Jinho Yeom, Jong Seong Park, Yong-Min Jeon, and

Seung Min Yoo*

School of Integrative Engineering, Chung-Ang University

G030

Innovative RNA Aptamer-based Extracellular Secretion

Platform for Chemical Production in Microbial Cell

Factories

Su Min Oh, Seoyoung Heo, Seung-Woon Jung, Tae Jeong Koo, and

Seung Min Yoo*

School of Integrative Engineering, Chung-Ang University

G031

Melanin Production and Characterization Using

Aspergillus niger CX-BI via Solid-state Fermentation

Hyunji Lee, Jeoungjin Ryu, and Seunghyun Kang*

Department of Research & Innovation Center, COSMAX BTI

G032

Platforms for High-throughput and High-content

Screening Campaigns in GBSA

Jungeun Yang, Jung Mi Hyun, Gyeong Been Lee, Eu Gene Jeong,

and Kwi Wan Jeong*

Gyeonggido Business & Science Accelerator

G033

Anti-inflammatory and Skin Health Effects of

Fermented Extracts of Houttuynia cordata

Gyeongbin Lee, Eugene Jeong, Jung Mi Hyun, Jungeun Yang, and

Kwiwan Jeong*

Biocenter, Gyeonggido Business and Science Accelerator

G034

Artificial Intelligence Approach for Identifying Key

Metabolic Reactions in Bacterial Cell Growth

Youngshin Kim, Hyunjae Woo, Dohyeon Kim, and Sung Ho Yoon*

Department of Bioscience and Biotechnology, Konkuk University

G035

Development of Genome-scale Metabolic Model of

Lactobacillus Species

Shakra Ahmad, Dohyeon Kim, Youngshin Kim, and Sung Ho Yoon*

Department of Bioscience and Biotechnology, Konkuk University

G036

Flavor Production Patterns of Kluyveromyces

marxianus Isolates in Beer Fermentation

ChanHaeng Lee1, So-Yeong Hwang2, Jeong-Ah Yoon3, and

Myoung-Dong Kim2,4*

1Department of Food Biotechnology, Kangwon National University, 2Department

of Food Science and Biotechnology, Kangwon National University, 3Department

of Food Biotechnology and Environmental Science, Kangwon National

University, 4Institute of Fermentation and Brewing, Kangwon National

University

G037

Development of Microbial Consortium for Plant Growth

Promotion and Phytopathogen Suppression in Soybean

Hyeongju Choi1, Jihyeon Ma1, Gahee Lee1, Woo Man An1,

Sung-won Park1, Gyuri Hong1, Hyun Gi Kong2, Pyung Il Kim3,

Young Kun Shim4, and Hyunkyu Sang1*

1Department of Integrative Food, Bioscience and Biotechnology, Chonnam

National University, 2Department of Plant Medicine, College of Agriculture,

Life and Environment Science, Chungbuk National University, 3Center for

Industrialization of Agricultural and Livestock Microorganisms, 4Microzyme

Poster

www.msk.or.kr | 269

G038

CRISPR-Cas Mediated Microbial Precise Genome Editing

by Modified Guide RNAs

Ho Joung Lee1, Hyun Ju Kim2, and Sang Jun Lee1*

1Department of Systems Biotechnology and Institute of Microbiomics,

Chung-Ang University, 2Biological Resources Research Department, Nakdonggang

National Institute of Biological Resources

G039

Heterologous Expression of Inner Membrane-bound

Protein, CbcL, Increases the Extracellular Electron

Transfer Rate of Cyanobacteria

Jonghwan Yoon and Ah-Ram Kim*

Handong Global University

G040

Optimization of pColdI Vector System for High Level

Expression of Flounder Growth Hormone in Escherichia

coli

Heejin Ahn, Su-Hyun Kim, Min-Kwan Sung, Sohyeon Jo, DoYoun

Jung, and Tae-Jin Choi*

Department of Microbiology and School of Marine and Fisheries Life

Sciences, Pukyong National University

G041

Expression of Virus-like Particles (VLPs) of Dengue

Virus Using Saccharomyces cerevisiae

Geonmin Kim1, Kum-Kang So2, Ngoc My Tieu Le1, Beom-Tae Kim3,

and Dae-Hyuk Kim1,2*

1Department of Bioactive Material Sciences, Jeonbuk National University,

2Institute for Molecular Biology and Genetics, Department of Molecular

Biology, Jeonbuk National University, 3Department of Bioenvironmental

Chemistry, Jeonbuk National University

G042

Whey Protein Fermented by Lacticaseibacillus

rhamnosus IM36 Enhances Prevention of

Dexamethasone-induced Myotube Atrophy

Jihun Jang, Hyeon Ho Jung, and Namsu Oh*

Korea University, Department of Food & Biotechnology

G043

Heterologous Gene Expression Using the Aspergillus

oryzae Strain Isolated from Korean Traditional

Fermented Food, Meju

Kap-Hoon Han, Hyun-Jun Park, Su-An Jeong, Kun Wang,

Seong-Hwan Lee, Hye-Rim Jeon, and Sang-Cheol Jun*

Department of Pharmaceutical Engineering, Woosuk University

G044

Genetic Analysis of Plasmid Isolated from Lactiplantibacillus

plantarum for Expression Vector Systems

Seon Yeop Kim and Eun Bae Kim*

Department of Applied Animal Science, College of Animal Life Science,

Kangwon National University

G045

Low-alcohol Beer Fermentation by Pichia fermentans

Hye Jin Hong1, Chan Haeng Lee2, Jung Ah Yoon3, Jang Woo Chun4,

and Myoung-Dong Kim1,5*

1Department of Food Science and Biotechnology, Kangwon National University,

2Department of Food Biotechnology, Kangwon National University,

3Department of Food Biotechnology and Environmental Science, Kangwon

National University, 4Hitejinro R&D Center, 5Institute of Fermentation and

Brewing, Kangwon National University

G046

Antimicrobial Activity of Plant Extracts from Mongolia

Hye-Jin Kim1, Ji-Yeon Lee1, Hye-Jeong Choi1, Ye-Jin Kang2, and

Hee-Soo Park1,2*

1School of Food Science and Biotechnology, Kyungpook National University,

2Department of Integrative Biology, Kyungpook National University

G047

Stress Tolerances of Saccharomyces cerevisiae Isolate

as Probiotic Candidate

Hye Jin Hong1, Min Kyung Ju1, Jung Ah Yoon2, Sang Min Shim3, and

Myoung Dong Kim1,4*

1Department of Food Science and Biotechnology, Kangwon National University,

2Department of Food Biotechnology and Environmental Science, Kangwon

National University, 3SPC Research Institute of Food and Biotechnology,

Seoul National University, 4Institute of Fermentation and Brewing, Kangwon

National University

G048

Proof-of-Concept Study: Upgrading GRAS to Probiotics

by Combining Beneficial Features

Ho-Seok Yoo1, Seol-Hwa Choi1, and Geun-Joong Kim2*

1Department of Biological Sciences, Chonnam National University, 2Department

of Biological Sciences, Chonnam National University

G049

Novel Production Method for Human Cytochrome

P450s: Reconstitution of Separate Proteins by Split

Intein

Su-Kyoung Yoo1 and Geun-Joong Kim2*

1Nurd GRAS Inc., 2Department of Biological Sciences, Chonnam National

University

2024 International Meeting of the Microbiological Society of Korea

270 | www.msk.or.kr

G050

Optimization of Promoter for GFPmut3b Expression in

Escherichia coli and Bacillus subtilis

Hyungbin Kim, Jae-Yoon Sung, and Dong-Woo Lee*

Department of Biotechnology, Yonsei University

G051

Characterization of Thermotoga neapolitana CTN0232

as a Non-phospho Sugar 4-Epimerase

MoonHo Choi and Dong-Woo Lee*

Department of Biotechnology, Yonsei University

G052

Gain-of-Function Mutation in the Galactitol-specific

GatC Transporter Facilitates Glucose Uptake in E. coli

Su On Jeong1, Hyun Ju Kim2, and Sang Jun Lee1*

1Department of Systems Biotechnology, and Institute of Microbiomics,

Chung-Ang University, 2Biological Resources Research Department, Nakdonggang

National Institute of Biological Resources

G053

Microbial Precise Genome Editing Using Truncated

sgRNA and Paired Cas9-NG Nickase

Song Hee Jeong, Ho Joung Lee, and Sang Jun Lee*

Department of Systems Biotechnology, Chung-Ang University

G054

Developing Oral Vaccines against Foot-and-Mouth

Disease Virus Using Saccharomyces cerevisiae

My Tieu Ngoc Le1, Kum-Kang So2, Geon-Min Kim1, Seung-Moon

Park3, and Dae-Hyuk Kim1,2*

1Department of Bioactive Material Sciences, Jeonbuk National University,

2Institute for Molecular Biology and Genetics, Department of Molecular

Biology, Jeonbuk National University, 3Department of Bioenvironmental

Chemistry, Jeonbuk National University

G055

Control of Lysogeny Formation in Escherichia coli Using

CRISPR-Cas12f1-Armed Synthetic λ Bacteriophages

Ho Joung Lee, Song Hee Jeong, and Sang Jun Lee*

Department of Systems Biotechnology and Institute of Microbiomics,

Chung-Ang University

G056

Polyhydroxyalkanoate Production in Halophilic Marine

Bacteria and Assessing Bioplastics Biodegradability

Sondor Ganbat1, Dariimaa Ganbat1, Yujin Son2, Ji Yeong Park1, Joo

Young Yang1, Dong-Woo Lee3, Sung Tae Kim4, Seong-Bo Kim5, and

Sang-Jae Lee1*

1Department of Food Biotechnology and Research Center for Extremophiles

and Marine Microbiology, Silla University, 2Department of Integrative

Biotechnology (IBT), Yonsei University, 3Department of Biotechnology,

Yonsei University, 4Department of Nanoscience and Engineering, Inje

University, 5Bio-Living Engineering Major, Global Leaders College, Yonsei

University

Poster

www.msk.or.kr | 271

H001

Utilization of Korean-type Reference Strain

Designation for Korean Industrial Standards

Microorganisms

Hohyun Jin, Jihye Kwak, Eunok Ko, Yongjae Yoo, Jeonmin Lee, and

Jinsik Cho*

Biological Testing Center, Korea Testing & Research Institute

H002

Combating Oral Pathogens: Food-derived Probiotic

Consortia Inhibit Cross-kingdom Biofilm Formation In

Vitro

Rajitha Kuthirakkal1, Byung Hee Ryu2, and Dongyeop Kim1*

1Department of Preventive Dentistry, School of Dentistry, Jeonbuk National

University, 2JONGGA R&D Product Division, Daesang Corporation

H003

Seroprevalence of Swine Acute Diarrhea Syndrome

Coronavirus in South Korea

Jung Eun Park, Yu-Jeong Jang, and Je Won Ko*

Chungnam National University

H004

Evaluation of Swine Non-invasive Field Sampling

Methods for Infectious Disease Surveillance

Hansong Chae and Jae Young Heo*

R&D Center of Animal Technology, Animal Industry Data Korea

H005

Interspecies Interaction Shapes and Alters Cariogenic

Community Assembly

Hee Young Jung, Jae Gyu Jeon, and Dongyeop Kim*

Department of Preventive Dentistry, School of Dentistry, Jeonbuk National

University

H006

Analysis of Food Poisoning Outbreaks Occurred in

Busan, 2023

Kyeonga Kim, Yejee Byun, Jiyoung Park, Younghee Kwon, Jaehoon

Jeong, Hyesun Hwang, and Hocheol Yun*

Busan Metropolitan City Institute of Health and Environment

H007

The Role of Commensal Streptococci Pyruvate Oxidase

in Inhibiting Streptococcus mutans-Candida albicans

Biofilm

Hongfei Shao, Jian-Na Cai, Hee-Young Jung, Jae-Gyu Jeon, and

Dongyeop Kim*

Jeonbuk National University

H008

Discovery of Tepoviruses Featuring a Nucleic

Acid-binding Protein

Dongjin Choi, Hyerin Park, Seungwoo Baek, Myeung Seok Choi,

and Yoonsoo Hahn*

Department of Life Science, Chung-Ang University

H009

Evaluating Ecological Dynamics in Oral Biofilm Models:

From Inter-Species Interactions to a Multi-species Community

Uyen Tran Thi To and Dongyeop Kim*

Biofilm Research Laboratory, Department of Preventive Dentistry, School

of Dentistry, Jeonbuk National University

H010

Erythrosine B Effectively Inhibits Streptococcus

mutans Biofilm Formation without Cytotoxic Impact on

Human Oral Epithelial Cells

Xuan Bach Ho, Hongfei Shao, and Dongyeop Kim*

Jeonbuk National University

H011

Iris pseudacorus L. Extracts Promote Glucose Uptake

via AMPK Pathway Activation in L6 Myotubes

Buyng Su Hwang, Ji Su Choi, Dae Won Jeong, Jeong Ho Kim, Min

Jin Kim, Young Taek Oh, Chang Hee Kang, and Yong Tae Jeong*

Nakdonggang National Institute of Biological Resources

H012

Analysis of Bias in Training Datasets for Antibiotic

Resistance Gene Prediction Models

Chan-Seok Jeong

Korea Institute of Science and Technology Information

H013

Comparative Analysis of Microbiome-based Food

Ingredient Safety Assessment Systems between the

Korea Ministry of Food and Drug Safety (MFDS) and

Food Standards Australia New Zealand (FSANZ)

Yeongjae Chae, Gi Beom Keum, Hyunok Doo, Jinok Kwak, Juyoun

Kang, Yejin Choi, Haram Kim, Sheena Kim, and Hyeun Bum Kim*

Department of Animal Biotechnology, Dankook University

H014

Safety Evaluation of M. multacida AGMB01124 as NGP

Candidate Strain Isolated from Healthy Porcine Feces

Seo-Joon Yeom1,2, JiHye Yang3, Min-ji Cho1,2, Beom-Seok Song1,

Jong-Heum Park1, Young-Min Kim2, Ju-Hoon Lee3, and Jae-Kyung

Kim1*

1Advanced Radiation Technology Institute, Korea Atomic Energy Research

Institute, 2Department of Integrative Food, Bioscience and Biotechnology,

Chonnam National University, 3Departments of Food and Animal Biotechnology

and Agricultural Biotechnology, Research Institute of Agriculture and Life

Sciences, Center for Food and Bioconvergence, Seoul National University

2024 International Meeting of the Microbiological Society of Korea

272 | www.msk.or.kr

H015

Bacterial Community Variation in Ruminant Feces

Across Host Age and Forage

Miye Kwon1, Yong Ho Jo2, Eungseok Kim2, and Weon-Jong Yoon1*

1Biodiversity Research Institute, Jeju Technopark, 2IANS Co., LTD.

H016

Growth Characteristics and Migration Analysis, HPLC

Analysis to Determine the Effect of Biotic Elicitor on

Damnacanthus major Calli

Eun Bi Jang, Jin Hwang, Young-Min Ham, Miye Kwon, So-yeon Oh,

Ho Bong Hyun, Boram Go, Jong-Du Lee, Weon-Jong Yoon, and

Ji-Gweon Park*

Biodiversity Research Institute, Clean Bio Business Division, Jeju Technopark

H017

Differences in Fecal Microbiome and Microbial

Networks by Obesity of Dogs

Ki Hyun Kim1, Kangmin Seo2, Julan Chun2, Min Young Lee2,

Sang-Yeob Lee2, Kyoung Min So2, and Hyun Woo Cho2*

1Academic-Industrial Cooperation Organization, Sunchon National University,

2Animal Welfare Research Team, National Institute of Animal Science

H018

Construction and In Vitro Characterization of

Recombinant SFTS Viruses Using a Reverse Genetics

Approach

Jihee Kim, Young-Eui Kim, Hae Ji Kang, Jungsang Ryou, and

Seok-Min Yun*

Division of Acute Viral Diseases, Center for Emerging Virus Research,

National Institute of Infectious Diseases, National Institute of Health, Korea

Disease Control and Prevention Agency

H019

Identification of Colletotrichum Species Causing

Anthracnose of Peaches and Evaluation of Fungicide

Resistance against the Pathogens

Sieun Kim, Ha-Kyoung Lee, Seong-Chan Lee, and You-Kyoung

Han*

Horticultural and Herbal Crop Environment Division, National Institute of

Horticultural & Herbal Science, Rural Development Administration

H020

Quantitative Analysis of Microbial Consortia for

Enhancing Tomato Growth and Improving Yield

through Effective Application and Persistence

Yong Ju Jin, Min Ju Lee, Jaekyeong Song, Hang-Yeon Weon, and

Mun-Jung Lee*

Agricultural Microbiology Division, National Institute of Agricultural Sciences,

Rural Development Administration

H021

Development of a Novel Next-generation Sequencing

(NGS) Panel Method for the Serogrouping of

Escherichia coli O-Serotypes and H-Serotypes Using

Nanopore

Jiseon Hwang1,2,3, Joon-Gi Kwon1,2,3, and Ju-Hoon Lee1,2,3,4*

1Department of Agricultural Biotechnology, Seoul National University,

2Research Institute of Agriculture and Life Sciences, Seoul National

University, 3Center for Food and Bioconvergence, Seoul National University,

4Department of Food and Animal Biotechnology, Seoul National University

H022

Evaluation of Changes in Gut Microbiota and

Digestibility in Cats Based on Protein Sources in Feed

Hyun-Jun Jang1, Jun-Hyeong Seo1, Young Bin Park2, and Sung Ho

Lee2*

1Department of Research and Development, Center for Industrialization of

Agricultural and Livestock Microorganisms, 2WOOGENE B&G Co., Ltd.

H023

Diagnosis of Similar Aerial Diseases in Watermelons

Mi-Kyung Kwon, Sung Won Yoon, Dasom Han, and Hyun Dong

Jang*

Fruit Vegetable Research Institute, Chungcheongnam-do Agricultural Research

and Extension Services

H024

Changes in Cognitive Improvement Compound Content

of Gastrodia elata Blume through Red Ginseng Vinegar

and Fermented Ginger Wine

Hwayoung Song, Ha Kyoung Choi, Sun Min Ju, Tae Young Kim, and

Dae II Hwang*

Institute of Jinan Red Ginseng

H025

Blood Microbiome Algorithm for Predicting Breast

Cancer Metastasis

Jieun Hong1, Yeoneun Kim1, Yunsoo Kang1, Donghyeok Choi2,3,4,

Sohyeon An5,6*, and Jeongshin An7,8*

1Ewha Womans University College of Medicine, 2Department of Medicine,

Yonsei University College of Medicine, 3Medical Physics and Biomedical

Engineering Lab (MPBEL), Yonsei University College of Medicine, 4Department

of Radiation Oncology, Yonsei Cancer Center, Heavy Ion Therapy Research

Institute, Yonsei University College of Medicine, 5Ewha Medical Research

Institute, School of Medicine, Ewha Womans University, 6Ewha Medical

Artificial Intelligence Research Institute, Ewha Womans University College

of Medicine, 7Department of Surgery, Ewha Womans University Mokdong

Hospital, School of Medicine, Ewha Womans University, 8Institute of

Convergence Medicine Research, Ewha Womans University Mokdong

Hospital, School of Medicine, Ewha Womans University

Poster

www.msk.or.kr | 273

H026

Longitudinal Study of Gut Microbiota Changes in Laying

Hens from Birth to Egg-laying

Seojin Choi and Eun Bae Kim*

Department of Applied Animal Science, College of Animal Life Science,

Kangwon National University

H027

Fluorophore-quencher-based Aptasensor: Real-time

Monitoring and Detection of Engineered Escherichia

coli in One-pot Screening System

Ki Beom Kim, Tae Yeong Jang, Sang Ho Kim, and Seung Min Yoo*

School of Integrative Engineering, Chung-Ang University

H028

Useful Components of Fermented Chaenomeles sinensis

by Lentinula edodes Mycelia Used as Herbal Medicine

Kyoung Sun Seo1, Kyung-Je Kim1, Seong-Woo Jin1, Young-Woo

Koh1, Seung-bin Im1, Neul I Ha1, Hee-Gyeong Jeong1, Sang-Wook

Jeong1, Kyeong-Won Yun2, Dong-Hyeon Lee1, Dong-Uk Kim1, and

Yu Jin Choi3*

1Jangheung Research Institute for Mushroom Industry, 2Department of

Oriental Medicine Resources, Sunchon National University, 3Imsil Cheese &

Food Institute

H029

Nanoconfined 3D Redox Capacitor-based Electrochemical

Sensor for Monitoring Bacterial Signaling Metabolite

and Detection of Bacterial Infection

Ki Beom Kim1, Mijeong Kang2, Chae Won Mun2, Ho Sang Jung2,

Dong-Ho Kim2, Sung-Gyu Park2, and Seung Min Yoo1*

1School of Integrative Engineering, Chung-Ang University, 2Advanced

Nano-Surface Department, Korea Institute of Materials Science (KIMS)

H030

Comparison of Functional Ingredient Contents

according to Lentinula edodes Cultivation Methods

Kyoung Sun Seo1, Kyung-Je Kim1, Seong-Woo Jin1, Young-Woo Koh1,

Neul I Ha1, Hee-Gyeong Jeong1, Sang-Wook Jeong1, Kyeong-Won

Yun2, Dong-Hyeon Lee1, Dong-Uk Kim1, and Yu Jin Choi3*

1Jangheung Research Institute for Mushroom Industry, 2Department of

Oriental Medicine Resources, Sunchon National University, 3Imsil Cheese &

Food Institute

H031

Chemical Components of the Korean Domestic

Lentinula edodes Strains and Imported Strains

Kyoung Sun Seo1, Kyung-Je Kim1, Seong-Woo Jin1, Young-Woo

Koh1, Seung-bin Im1, Neul I Ha1, Hee-Gyeong Jeong1, Sang-Wook

Jeong1, Dong-Hyeon Lee1, Dong-Uk Kim1, Kyeng-Won Yun2, and

Yu Jin Choi3*

1Jangheung Research Institute for Mushroom Industry, 2Department of

Oriental Medicine Resources, Sunchon National University, 3Imsil Cheese &

Food Institute

H032

Antimicrobial Resistance and Distribution of

Pathogenic E. coli Isolated from Raw Meat Sold in

Chungcheongnam-do

Namwoo Kim, Hyunah Lee, Dayeon Lee, and Junhyuk Park*

Chungcheongnam-do Institute of Health and Environment Research

H033

Strain Safety Test and Mass Cultivation Optimization

Study for Commercialization of Limosilactobacillus

fermentum 2L Strain Effective for Stress-resilience

Jun Hyeok Yang1, So Jin Lee1, Hye Ji Kim1, Da Young Lee1, Jong

Kook Moon1, Heui Kyoung Cho1, Seokyoung An2, Sae Hun Kim2,

and Ki Na Yun1*

1BTSynergy Co., Ltd., 2Department of Food Bioscience and Technology,

College of Life Science and Biotechnology, Korea University

H034

Optimization of Culture Condition, Characterization

and Safety Assessment of Clostridium butyricum

Isolated from Feces of Korea

Hyeji Kim1, Sojin Lee1, Junhyeok Yang1, Dayoung Lee1, Kina Yun1,

Jongkook Moon1, Dong-Woo Lee2, Hong Koh3, And Heuikyoung

Cho1*

1BTSynergy Co., Ltd., 2Department of Biotechnology, Yonsei University,

3Division of Gastroenterology, Hepatology and Nutrition, Department of

Pediatrics, Severance Hospital, Yonsei University College of Medicine

H035

Insights into the Composition of Bacteria, Fungi, and

Archaea in the Rumen Microbiome of Hanwoo

Soyoung Choi1, MinjiS Song1, Seul-A Jeong1, Woncheoul Park1,

Jin-A Lim1, Haesu Ko1, Na-Yeon Kim1, Sun-Sik Chang2, Youl-Chang

Baek2, Seong Shin Lee3, and Nam-Young Kim1*

1Animal Genomics and Bioinformatics Division, National Institute of Animal

Science, Rural Development Administration, 2Hanwoo Research Institute,

National Institute of Animal Science, Rural Development Administration,

3Animal Nutrition & Physiology Division, National Institute of Animal

Science, Rural Development Administration

H036

Modeling Genomic Features to Identify Bacterial

Strains for Sustainable Food Waste Processing

Daool Park and Jaeyoung Choi*

Department of Oriental Medicine Biotechnology, College of Life Sciences,

Kyung Hee University

H037

The 5’ Proximal Sequence of Non-structural Protein 1 in

SARS-CoV-2 Plays a Significant Role in Regulating

Translational Efficiency Mediated by 5’ Untranslated Region

Ho-yeong Yoon and Jinjong Myoung*

Korea Zoonosis Research Institute, Jeonbuk National University

2024 International Meeting of the Microbiological Society of Korea

274 | www.msk.or.kr

H038

Country-specific Peptides of 207 African Swine Fever

Virus (ASFV) Genomes

Sieun Kim and Eun Bae Kim*

Department of Applied Animal Science, College of Animal Life Science,

Kangwon National University

H039

Strategy for Shortening Time to Drug Susceptibility

Test for Mycobacterium tuberculosis by Combining

PCR- and Culture-based Diagnostic Methods

Hyeon-jeong Lee, Sungkyoung Lee, and Tae-hyoun Kim*

Division of Bacterial Disease Research, Center for Infectious Disease Research,

Korea National Institute of Health

H040

Plastics and Prions in Yeast: The Impact of Microplastics

on Prion Generation in Saccharomyces cerevisiae

Seongje Yuk1 and Moonil Son1,2,3*

1Department of Integrated Biological Science, Pusan National University,

2Department of Microbiology, Pusan National University, 3Microbiological

Resource Research Institute, Pusan National University

H041

Identification of Novel Yeast Prion Protein Binding

Partners with Anti-prion Activity

Suyeon Cho1 and Moonil Son1,2,3*

1Department of Integrated Biological Science, Pusan National University,

2Department of Microbiology, Pusan National University, 3Microbiological

Resource Research Institute, Pusan National University

H042

Structural Interplay of Transcription Regulatory

Sequences and Proximal Flanking Regions Modulates

SARS-CoV-2 Gene Expression

Sojung Bae and Jinjong Myoung*

Molecular Virology Laboratory, Korea Zoonosis Research Institute, Jeonbuk

National University

H043

In Silico Analysis, Microbial Expression, and Enzymatic

Activity Assay of a Novel Chitinase of Eurytemora

affinis Copepods

Lintang Satyawan Ignatius, Catia Mota, Su Jin Yoo, Seong-il Eyun,

and Hyun-ah Kang *

Department of Life Science, Chung-Ang University

H044

2023 Operation of Laboratory Network for Response

to Bioterrorism in Chungcheongnam-do

Jihee Kim, Myeonghee Shin, and Hyeon-jung Kim*

Chungcheongnam-do Institute of Health and Environment Research

H045

Longitudinal, Integrative Metagenomic and

Metabolomic Analyses Reveal Gut Microbe-metabolism

Alteration in Ulcerative Colitis Patients Corresponding

to Clinical Outcomes of FMT

So-Yeon Yang1, Kyoung Su Kim1, Hyun Ryul Bak1, Hong Koh2, and

Dong-Woo Lee1*

1Department of Biotechnology, Yonsei University, 2Department of Pediatrics,

Yonsei University College of Medicine, Severance Fecal Microbiota Transplantation

Center, Severance Hospital

H046

The Generation and Analysis of the XBB.1.5

Recombinant Virus through an Advanced Reverse

Genetics Platform

Taesoo Kim and Jinjong Myoung*

Molecular Virology, Korea Zoonosis Research Institute, Jeonbuk National

University

H047

Development of a Highly Effective Rationally Designed

Live Attenuated Vaccine against SARS-CoV-2

Jisu Min and Jinjong Myoung*

Korea Zoonosis Research Institute, Jeonbuk National University

H048

Antiviral Control Effect Photofunctional Polymer Films

Rye Gyeong Park1, Ji Hyeon Lee1, Soo Hyun Han1, Il Kwon Bae2,

and Jae Hak Sohn1,3*

1Department of Food Science and Culinary Arts, Silla University, 2Department

of Companion Animals, Silla University, 3Seafood Research Center, IACF, Silla

University

H049

Metabolic Analysis of Extracellular Vesicle Isolation

with Different Filtration Methods

Myeongwon Lim1, Seong-Hun Bong1, Yu Mi Kim1, and Do Yup

Lee1,2,3*

1Department of Agricultural Biotechnology, Seoul National University,

2Department of Food and Animal Biotechnology, Seoul National University,

3Center for Food and Bioconvergence, Research Institute for Agricultural

and Life Sciences, Interdisciplinary Programs in Agricultural Genomics, Seoul

National University

H050

Dysbiosis of Oral and Gut Microbiomes in a Mouse

Model of Oral Squamous Cell Carcinoma

Euon Jung Tak, Beom-Jin Goo, Jae-Yun Lee, JeongEun Han,

Yun-Seok Jeong, Hae-In Joe, Hojun Sung, Hyun Sik Kim, and

Jin-Woo Bae*

Kyung Hee University

Poster

www.msk.or.kr | 275

H051

Integrating Fecal Incubation Systems with Machine

Learning to Investigate the Influence of Diet on Gut

Microbiota

Gyungcheon Kim, WonJune Lee, Juhui Jang, and Hakdong Shin*

Department of Food Science & Biotechnology, and Carbohydrate Bioproduct

Research Center, Sejong University

Exhibition

2024 International Meeting of the Microbiological Society of Korea

278 | www.msk.or.kr

Exhibition

∙Date: 10.30(Wed)-11.01(Fri)

∙Place: ICCJEJU 3F

Exhibition Layout

Exhibition

www.msk.or.kr | 279

Exhibitors

01. ㈜

파이젠

/ PHYZEN

02.

국립해양생물자원관 해양수산 바이오데이터 센터 사업단

/ MOBiDIC (Marine Organismal Big Data and

Information Center)

03.

해양미생물거점 기탁등록보존기관

/ MMRB (Marine Microbial Resource Bank)

04,05.

자연과학

㈜ / CHAYON Laboratories, Inc.

06. ㈜

마크로젠

/ Macrogen, Inc.

07. ㈜

어큐진

/ AccuGene, Inc.

08.

베르티스

/ Bertis

09. ㈜

이바이오젠

/ EBIOGEN, Inc.

10.

뉴잉글랜드바이오랩스

/ New England Biolabs

11. ㈜

서포터랩

/ SUPPORTERLAB, Inc.

12.

몰레큘러디바이시스코리아

(

유

) / Molecular Devices Korea

13.

케이비티

㈜ / Korea Bio-Tech Co., Ltd.

14.

아토플렉스

㈜ / ATTOPLEX

15. ㈜

유바이오젠

/ Eubiogene

16.

환경미생물은행

/ KEMB (Korea Environmental Microorganisms Bank)

17. ㈜

인실리코젠

/ Insilicogen, Inc.

18. ㈜

진시스템

/ Genesystem Co., Ltd.

19. ㈜

디엔에이링크

/ DNA LINK, Inc.

20.

시노바이오로지컬코리아

/ Sinobiological Korea

21. ㈜

바질바이오텍

/ Basil Biotech Co., Ltd.

22.

천지인바이오텍

/ CHUNJIIN Biotech Co., Ltd.

23. ㈜

에이스엠자임

/ AceEMzyme Co., Ltd.

24.

한국화학연구원 감염병기술전략센터

/ SID (Strategy and Policy Center for Infectious Diseases)

25.

주식회사 크로니텍

/ CRONYTEK

26,27. ㈜

바이오니아

/ Bioneer Corporation

28. ㈜

마이크로자임

/ Microzyme

29. ㈜

비엠에스

/ BMS (Bio-Medical Science Co., Ltd.)

30.

고마바이오텍

㈜ / KOMA BIOTECH

31. ㈜

셀투바이오

/ CelltoBio

32.

국립해양생물자원관

/ MABIK (National Marine Biodiversity Institute of Korea)

33.

일루미나 코리아

/ Illumina Korea, Inc.

2024 International Meeting of the Microbiological Society of Korea

280 | www.msk.or.kr

34.

씨제이바이오사이언스

㈜ / CJ Bioscience, Inc.

35. CJ

제일제당

/ CJ CheilJedang

36.

클리니컬 마이크로바이오믹스

/ Clinical-Microbiomics

37. ㈜

쎌바이오텍

/ CELL BIOTECH

38,39. ㈜

세니젠

/ Sanigen Co., Ltd.

40.

질병관리청 국립보건연구원 국가병원체자원은행

/ NCCP (National Culture Collection for Pathogens,

Korea National Institute of Health)

41,42.

기산바이오

㈜ / KisanBio Co., Ltd.

43. ㈜

제노믹베이스

/ GenomicBase Co., Ltd.

44. ㈜

엘에이에스

/ LAS

45.

한국해양과학기술원

/ Korea Institute of Ocean Science and Technology

Exhibition

www.msk.or.kr | 281

PHYZEN Booth No. 1

㈜파이젠

CEO: Taeseok Park

Homepage: www.phyzen.com

Address: 605, Baekgoong Plaza1, 13 Seongnam-daero 331beon-gil, Bundang-gu Seongnam-si, Gyeonggi-do 13558,

Korea

Main Technology and Products

PHYZEN is a company specializing in biological information analysis based on omics data. We are analyzing DNA/RNA base

sequences by introducing the latest genome analysis system, and developing our own program for bioinformation

analysis, and building a bioinformation DB and artificial intelligence-based analysis system.

MOBiDIC (Marine Organismal Big Data and Information Center) Booth No. 02

국립해양생물자원관 해양수산 바이오데이터 센터 사업단

CEO: Jeong Hyeon Choi

Homepage: www.mabik.re.kr

Address: 75 Jangsan-ro 101beon-gil, Janghang-eup, Seochun-gun, Chungchungnam-do 33662, Korea

Main Technology and Products

The Marine Organismal Big Data and Information Center (MOBiDIC) stands at the forefront of marine bio-research,

functioning as a pivotal hub for the collection, management, analysis, and dissemination of marine biological data.

Established under the auspices of the Ministry of Oceans and Fisheries, the MOBiDIC aggregates extensive research and

development data pertaining to marine organisms. This centralized repository serves as an invaluable resource for

scientists, researchers, and policymakers engaged in marine and environmental sciences.

MMRB (Marine Microbial Resource Bank) Booth No. 03

해양미생물거점 기탁등록보존기관

CEO: Young Woon Lim

Homepage: www.mbris.kr

Address: 502-403, School of Biological Sciences, College of Natural Sciences, Seoul National University. 1 Gwanak-ro,

Gwanak-gu, Seoul 08826, Korea

Main Technology and Products

Marine Microbial Resource Bank (MMRB) is discovering marine microorganisms (Bacteria and Fungi) from various marine

substrates. Isolated microorganisms are stored in our institutes and given to researchers for further research. Look it up

on MBRIS (www.mbris.kr).

CHAYON Laboratories, Inc. Booth No. 04,05

자연과학㈜

CEO: Sunki Kim, Minah Lee

Homepage: www.chayon.co.kr

Address: 22, Yeoksam-ro 7-gil, Gangnam-gu, Seoul 06244, Korea

Main Technology and Products

Since 1997, CHAYON Laboratories Inc. has been introducing the latest technologies and products for life sciences and

other related research areas. Based on the decades of systematic support by our in-house Technical Sales&Application

Scientists, CHAYON is focused on providing unrivaled drug development/discovery services in the Drug Discovery area. In

addition, CHAYON is leading the market in various research fields by supplying most up-to-date molecular and cell biology

related products, as well as pre-clinical imaging and other automation equipment that incorporate the latest technologies.

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Macrogen, Inc. Booth No. 06

㈜마크로젠

CEO: Chang-Hoon Kim

Homepage: www.macrogen.com

Address: 238 Teheran-ro, Gangnam-gu, Seoul 06221, Korea

Main Technology and Products

Macrogen is a global leader in genomic analysis and personalized healthcare, with a strong presence in Europe, Asia, and

North America. The company offers advanced services such as NGS-based genomic analysis, microbiome solutions, and

single-cell analysis, aiming to drive precision medicine. Macrogen is expanding its global network to enhance accessibility

and service quality. It collaborates with over 18,000 research clients across 153 countries, contributing to the

advancement of bioindustries through innovative R&D and CSR activities. The company is also focusing on new business

models, including AI platforms and data-driven healthcare solutions.

AccuGene, Inc. Booth No. 07

㈜어큐진

CEO: Soon Jae Kwon

Homepage: www.accugenelab.com

Address: 73, Gaetbeol-ro, Yeonsu-gu, Incheon 21999, Korea

Main Technology and Products

Our microbiome analysis is conducted using Next-Generation Sequencing(NGS) to analyze the details of microbiota that

are found in the collected sample such as the microbiome type and quantity. This data is used to generate personalized

health and gut health improvement solutions that are specifically geared towards the individual’s microbiome profile.

Bertis Booth No. 08

베르티스

CEO: Seungman Han, Dongyoung Noh

Homepage: www.bertis.com

Address: 6F, ARTISAN Bldg, 614, Nonhyeon-ro, Gangnam-gu, Seoul 06108, Korea

Main Technology and Products

Bertis is an innovator in the proteomics industry, leveraging unique technology to broaden the scope of precision

medicine from early diagnosis to drug target discovery.The company has succeeded in commercializing proteomics-based

breast cancer blood testing solution and multi-omics analysis service.

EBIOGEN, Inc. Booth No. 09

㈜이바이오젠

CEO: Seoung Duck Ryu

Homepage: www.e-biogen.com

Address: #405, 48, Ttukseom-ro 17ga-gil, Seongdong-gu, Seoul 04785, Korea

Main Technology and Products

Ebiogen was founded in 2007 and our main technologies are NGS sequencing(RNA-seq, DNA-seq), Proteomics

analysis(LC-MS/MS, Antibody array) and ExDEGA data analysis. With the vision of “BioResearch & Healthcare Creator”, we

conduct research and development of healthcare related products and provide experiment-analysis services for biological

researchers to achieve good research results.

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New England Biolabs Booth No. 10

뉴잉글랜드바이오랩스

CEO: Sue Yap Soh Mooi

Homepage: www.neb.com

Address: 3 A 709, Goseong-ro, Seongnam-si, Gyeonggi-do 13105, Korea

Main Technology and Products

NGS-based Molecular Diagnostics, Amplification-based Molecular Diagnostics, mRNA/RNA Synthesis Established in 1974,

New England Biolabs is the industry leader in the discovery and production of enzymes for molecular biology applications

and now offers the largest selection of recombinant and native enzymes for genomic research.

SUPPORTERLAB, Inc. Booth No. 11

㈜서포터랩

CEO: Junyoung Joo

Address: 26, Geumo 3-gil, Dong-myeon, Yangsan-si, Gyeongsangnam-do 50639, Korea

Main Technology and Products

SupporterLab is a dedicated partner, always supporting our clients' growth and success.

We prioritize being a reliable company, offering personalized support, and consistently striving to meet our clients' needs.

As a trustworthy partner, SupporterLab is committed to standing by our clients and leading the way toward sustainable

growth.

Molecular Devices Korea Booth No. 12

몰레큘러디바이시스코리아(유)

CEO: Kyungtae Seo

Homepage: ko.moleculardevices.com

Address: 15F Samsung Bldg. 623 Teheran-ro, Gangnam-gu, Seoul 06173, Korea

Main Technology and Products

Welcome to Molecular Devices, a Danaher Life Sciences Group company.

Molecular Devices is headquartered in San Jose, California, USA.

We are a leading High-Content Imaging company focused on drug screening and biomimetic tissue development based on

Microplate Reader, 3D imaging and artificial intelligence(AI).

and development of biomimetic tissues based on 3D imaging and AI.

Korea Bio-Tech Co., Ltd. Booth No. 13

케이비티㈜

CEO: Hyeong Kil Kim

Homepage: www.kbt.co.kr

Address: 5, Dongwon-ro 21-gil, Bundang-Gu, Sungnam-Si, Gyeonggi-Do 13547, Korea

Main Technology and Products

Cell/Gel Imaging System, Mixer/Shaker, Incubator, Sonicator, Electrophoresis system, Spectrometer, Microscope, Speed

Vac, Freeze Dryer, etc.

ATTOPLEX Booth No. 14

아토플렉스㈜

CEO: Hyun Kyu Yoon, In Hyuk Chung

Homepage: www.attoplex.co.kr

Address: F913, 45, Jojeong-daero, Hanam-si, Gyeonggi-do 12918, Korea

Main Technology and Products

Attoplex has completed the development of a total of 136 molecular diagnostics items, including animal/plant Pathogens,

foodborne pathogens, and bioterrorism agents. Based on it, Attoplex contributes to researching and developing

non-contact diagnostics system that can be used by anyone easily, quickly, accurately, and at a low cost.

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Eubiogene Booth No. 15

㈜유바이오젠

CEO: Whan Chul Shin

Homepage: www.eubiogene.com

Address: B-Bldg #1104, Samho Company LTD., 87, Nonhyeon-ro, Seocho-gu, Seoul 06775, Korea

Main Technology and Products

Eubiogene is a specialized company handling experimental equipment. We provides the service in the field of university

lab, biotechnology labs, government offices, and food research institutes. And expanding its sales forces towards the

business of diagnosis market such as pharmaceuticals and examination center. We will always research and fulfill our

promises in the step of the fast growing trend of the biotechnology. Please watch us growing into a leading company in

the country.

KEMB (Korea Environmental Microorganisms Bank) Booth No. 16

환경미생물은행

CEO: Sang-Seob Lee

Homepage: kemb.or.kr

Address: Natural Sciences 2 (32), 2066 Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do 16419, Korea

Main Technology and Products

The Korea Environmental Microorganisms Bank (KEMB) was established as a microbial and genetic resource center for the

development and conservation of microbial resources. The goals of KEMB are; 1) Collection and conservation of native

environmental microorganisms and genetic resources 2) Construction of systematic management system for effective

conservation and application of microbiological resources for environmental industries 3) Providing data for ecosystem

research and microbial classification 4) Providing data for bioremediation of environmental pollutant and ecosystem

restoration

Insilicogen, Inc. Booth No. 17

㈜인실리코젠

CEO: Namwoo Choi

Homepage: insilicogen.com

Address: #2901~2904, Tower-dong A, HEUNGDEOK IT VALLEY, 13, Heungdeok 1-ro, Giheung-gu, Yongin-si,

Gyeonggi-do 16954, Korea

Main Technology and Products

Insilicogen has continuously constructed the bio big data for the past 20 years.

We will go one step deeper into this trend. Insilicogen will be the headstone of big data and will provide the most

productive and effective process with the know-how that has been acquired through the development of data

production, archiving, modeling and analysis.

Genesystem Co., Ltd. Booth No. 18

㈜진시스템

CEO: Yujin Seo

Homepage: www.genesystem.co.kr

Address: 200-9, Techno 2-ro, Yuseong-gu, Daejeon 34028, Korea

Main Technology and Products

Genesystem is a biotech engineering company, specializing in rapid on-site molecular diagnostic platforms, creating a

sustainable future with state-of-the-art diagnostic technology.

Exhibition

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DNA LINK, Inc. Booth No. 19

㈜디엔에이링크

CEO: Jongeun Lee

Homepage: www.dnalink.com

Address: ASSA Building, 31, Magokjuang 8-Ro 3-Gil Gangseo-Gu, Seoul 07793, Korea

Main Technology and Products

We are one of the earliest NGS service providers who obtained extensive experience since we founded our company in

2000. Over the last two decades, we sequenced and analyzed more than 1,000 genomes from diverse sources. We

received CSP accreditation including Illumina, Pacific Biosciences, 10X Genomics, and Life Technologies & Affymetrix.

Sinobiological Korea Booth No. 20

시노바이오로지컬코리아

CEO: Wenyi Che

Homepage: www.sinobiological.com

Address: West 609, 295, Gwangpyeong-ro, Gangnam-gu, Seoul 06349, Korea

Main Technology and Products

Sinobiological is an international reagent supplier and service provider. The company specializes in recombinant protein

production and antibody development.

Basil Biotech Co., Ltd. Booth No. 21

㈜바질바이오텍

CEO: Jong Moon Park

Homepage: www.basilbiotech.com

Address: #3302, Techno Park IT Center, 32 Songdogwahak-ro, Yeonsu-gu, Incheon 21984, Korea

Main Technology and Products

It provides services such as proteomics, metabolomics, lipidomics, glycomics analysis, as well as automated sample

purification and separation systems. In addition, it provides high-quality research services with liquid chromatography,

mass spectrometry, and sample automation systems capable of processing hundreds of samples at a time.

CHUNJIIN Biotech Co., Ltd. Booth No. 22

천지인바이오텍

CEO: Byung-Oh Kwon

Homepage: www.chunjiinbt.com

Address: 16-25, Cheoramgongdan-gil, Taebaek-si, Gangwon-do 26046, Korea

Main Technology and Products

Founded in 2015 based on pesticide manufacturing, it has eight pesticide sales branches and one bio business branch

nationwide, achieving stable annual sales of 15 billion won. In addition to chemical pesticides, it is a company that

manufactures microbial pesticides, fertilizers, and eco-friendly products.

AceEMzyme Co., Ltd. Booth No. 23

㈜에이스엠자임

CEO: Wan-Taek Im

Homepage: www.aceemzyme.kr

Address: #403, Industry-Academic Cooperation Building, Hankyong National University, 327 Jungang-ro, Anseong-si,

Gyeonggi-do 17579, Korea

Main Technology and Products

Identification of microorganism, Bulk culture [EM], Special enzyme production technology, Bioconversion technology,

Mass production technology in the unit of kg or higher, Separation technology of natural substances, Standard

manufacturing technology, Substance analysis technology

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SID (Strategy and Policy Center for Infectious Diseases) Booth No. 24

한국화학연구원 감염병기술전략센터

CEO: Young Kuk Lee

Homepage: www.krict.re.kr

Address: 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, Korea

Main Technology and Products

Strategy & Policy Center for Infectious Diseases(SID) was established in July 2022 at the Korea Research Institute of

Chemical Technology, with the support of the Ministry of Science and ICT. SID is committed to attaining technological

independence in foundational core technologies across the six major areas of infectious diseases (basic research,

prevention, treatment, diagnosis, surveillance and prediction, infrastructure) and to building scientific and technological

capacities for rapid response to future infectious diseases.

CRONYTEK Booth No. 25

주식회사 크로니텍

CEO: Jae Wan Lee

Homepage: www.cronytek.com

Address: HIFIELD A-#811, 66, Beolmal-ro, Dongan-gu, Anyang-si, Gyeonggi-do 14058, Korea

Main Technology and Products

As a company that specializes in basic research and bio products necessary for life science research, it has been steadily

growing in the lifescience tools distribution industry with the corporate philosophy of 'trust' since it's establishment in

2013

Bioneer Corporation Booth No. 26, 27

㈜바이오니아

CEO: Han Oh Park

Homepage: www.bioneer.com

Address: 71, Techno 2-ro, Yuseong-gu, Daejeon 34013, Korea

Main Technology and Products

Since its establishment in 1992, Bioneer has been dedicated to localizing biotechnology research products. Over the

years, we have successfully developed and commercialized PCR enzymes and primers (synthetic DNA), PCR, and Real-time

PCR instruments, marking significant milestones as the first in Korea. Additionally, we provide various services such as

sequencing, NGS, gene and protein synthesis, and gene expression analysis.

Microzyme Booth No. 28

㈜마이크로자임

CEO: Yong Keun Shim

Homepage: www.microzyme.co.kr

Address: 37-1, Wondeung 1-gil, Goseo-myeon, Damyang-gun, Jeollanam-do 57385, Korea

Main Technology and Products

Microzyme is an abbreviation for microorganism + enzyme and is a company that develops cultivation technology for

agriculture, fisheries and livestock based on various biological resources. Our main products include organic farming

materials for domestic plant disease control, root-knot nematode and flying pest control, GABA, and fertilizers that can

produce functional crops.

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BMS (Bio-Medical Science Co., Ltd.) Booth No. 29

㈜비엠에스

CEO: Sunki Kim

Homepage: www.bms.kr

Address: 22 Yeoksam-ro, Gangnam-gu, Seoul 06244, Korea

Main Technology and Products

Since 1988, Bio-Medical Science Co., Ltd. has been conducting research in life sciences and basic sciences. By introducing

the latest technology and products into Korea, We have been constantly striving to contribute to the development of life

science research, Expanding its scope to basic research equipment manufacturing and development, as a partner in life

science research Over and over again, new roles are being played.

KOMA BIOTECH Booth No. 30

고마바이오텍㈜

CEO: Sang Hoon Moon

Homepage: www.komabiotech.co.kr

Address: #1907, IS BIZ Tower, Yangpyeong-ro 21 gil 26, Yeongdeungpo-gu, Seoul 07207, Korea

Main Technology and Products

KOMABIOTECH manufactures and provides reagents and tools for life science research.

The comprehensive product lines support applications in the study of all major life science research areas, including

molecular biology,immunology, cell biology, microbiology research.

CelltoBio Booth No. 31

㈜셀투바이오

CEO: Jun Won Mo

Homepage: www.cellto.co.kr

Address: 474, Dunchon-daero, Jungwon-gu, Seongnam-si, Gyeonggi-do 13229, Korea

Main Technology and Products

We provide domestic and foreign research reagents and consumables, physical and chemical equipment, medical devices,

and medical products to many bio industries.

MABIK (National Marine Biodiversity Institute of Korea) Booth No. 32

국립해양생물자원관

CEO: Wan-hyun Choi

Homepage: www.mbris.kr

Address: Marine BioBank 75, jangsan-ro, 101beon-gil, Janghang-eup, Seocheon-gun, Chungcheongnam-do 33662, Korea

Main Technology and Products

Marine BioBank aims to investigate potential marine bio-resources for industrialization and support the stakeholders from

industries, academics and research fields by giving information and distributing marine biomaterials. Ministry of Oceans

and Fisheries and National Marine Biodiversity Institute of Korea(MABIK) play vital roles in leading the 4th industrial

revolution by creating the value of marine bio-resoruces.

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Illumina Korea, Inc. Booth No. 33

일루미나 코리아

CEO: Rob Kenneth McBride

Homepage: www.illumina.com

Address: 66 Yeoidae-ro, Yeoungdeungpo-gu, Seoul 07325, Korea

Main Technology and Products

At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies

possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and

scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative

interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. Illumina

innovative sequencing and array technologies are fueling groundbreaking advancements in life science research,

translational and consumer genomics, and molecular diagnostics.

CJ Bioscience, Inc. Booth No. 34

씨제이바이오사이언스㈜

CEO: Jongsik Chun

Homepage: www.cjbioscience.com

Address: 14 Sejong-daero, Jung-gu, Seoul 04527, Korea

Main Technology and Products

CJ Bioscience provides solutions to all areas of microbiological research, including biomedicien, institutional R&D, and

genetic sequencing with our unique combination of biology and information technology. We serve a reference database,

EzBioCloud, for bacterial taxonomy, 16S marker genes, and bacterial genomes.

CJ CheilJedang Booth No. 35

CJ제일제당

CEO: Sin Ho Kang

Homepage: cj.co.kr

Address: 55, Gwanggyo-ro 42beon-gil, Yeongtong-gu, Suwon-si, Gyeonggi-do 16495, Korea

Main Technology and Products

CJ BIO oversees the Animal Nutrition&Health and Taste&Nutrition business using exceptional fermentation and

refinement tech. Furthermore, CJ BIO is achieving results not only in the development of Amino Acid but also in Enzyme

and PHA, by integrating new AI-DT tech. with our own expertise and knowhow of Synthetic biology & Bio-process

technology fields.

Clinical-Microbiomics Booth No. 36

클리니컬 마이크로바이오믹스

CEO: Anders Gron

Homepage: clinical-microbiomics.com

Address: Fruebjergvej 3, 2100 København, Denmark

Main Technology and Products

CMC provides microbiome multi-omics platform, we can accurately analyze microbiome (taxonomical and functional) and

metabolome differences between samples – from different treatment groups to differences between healthy individuals

and those with disease. We support clinical and pre-clinical studies with end-to-end metagenomics and metabolomics

services, leading bioinformatics solutions and advanced biostatistics linking the microbiome and metabolome to clinical

data and outcomes.

Exhibition

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CELL BIOTECH Booth No. 37

㈜쎌바이오텍

CEO: Myung-Jun Chung

Homepage: www.cellbiotech.com

Address: 50, Aegibong-ro 409 beon-gil, Gaegok-ri, Wolgot-myeon, Gimpo-si, Gyeonggi-do 10003, Korea

Main Technology and Products

Since its foundation by microbiologists in 1995, we have over 3 decades of experience in the pharmaceutical industry and

are ready to provide a full service to our partners with personalized, innovative, scientifically documented probiotic

solutions and marketing services.

Sanigen Co., Ltd. Booth No. 38,39

㈜세니젠

CEO: Jeong Woong Park

Homepage: www.sanigen.kr

Address: #411 Pyeongchon Digital Empire, 16, Heungan-daero 427beon-gil, Dongan-gu, Anyang-si, Gyeonggi-do 14059,

Korea

Main Technology and Products

* Geneka™(NGS Analysis Service)

* GeNext™(NGS Analysis System)

* Genelix™Real-time PCR Detection kit

* ForLabs(simple bag,simple diluent,sani-macroswab)

NCCP (National Culture Collection for Pathogens, Korea National Institute of Health) Booth No. 40

질병관리청 국립보건연구원 국가병원체자원은행

CEO: Gi-Eun Rhie

Homepage: nccp.kdca.go.kr

Address: 220 Osongsaengmyeong2-ro, Osong-eup, heunheung-gu, Cheongju-si, Chungcheongbuk-do 28160, Korea

Main Technology and Products

- Collection, receipt of deposits, analysis, and evaluation of pathogen resources

- Management, utilization, and distribution of pathogen resources

- Assistance for operation of specialized pathogen resource bank

- Construction and operation of a cooperation network of domestic institutions and foreign institutions related to

pathogen resource

- Establishment and operation of the pathogen resource information system

KisanBio Co., Ltd. Booth No. 41,42

기산바이오㈜

CEO: Ji Woon Sun

Homepage: www.kisanbio.com

Address: 11, Yangjaecheon-ro 31-gil, Seocho-Gu, Seoul 06746, Korea

Main Technology and Products

Kisanbio is microbial culture medium specialist that supplies high quality microbial medium “MBcell”. We have been

supplying customized medium, and standard medium that is suitable for domestic and foreign Test method; such as Food

Chemical Codex and Pharmacopoeia. We also provides Microbial Identification Test Kit and Bacteriological Test Reagent,

as well as professional academic consultations on the whole product. We will do our best to repay you with the best

quality and best service.

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GenomicBase Co., Ltd. Booth No. 43

㈜제노믹베이스

CEO: Dae Yoen Son

Homepage: www.genomicbase.com

Address: #F315, 25-23, Dasanjungang-ro 19 beon-gil, Namyangju-si, Gyeonggi-do 12248, Korea

Main Technology and Products

It is a company that sells related kits, reagents, and scientific equipment in the biotechnology/biopharmaceutical field.

LAS Booth No. 44

㈜엘에이에스

CEO: Dongho Kim

Homepage: www.las.kr

Address: 6F, 16, Arayuk-ro, Gochon-eup, Gimpo-si, Gyeonggi-do 10136, Korea

Main Technology and Products

LAS specializes in genetic information analysis to help predict, diagnose and prevent diseases using genetic information

differences.LAS currently has over 300 researchers and institutions as partners.

We do our best to provide reliable results.

Korea Institute of Ocean Science and Technology Booth No. 45

한국해양과학기술원

CEO: Hyi Seung Lee

Homepage: www.kiost.ac.kr

Address: 385, Haeyang-ro, Yeongdo-gu, Busan 49111, Korea

Main Technology and Products

KIOST aims to guide Korea toward fulfilling its Dream of becoming a maritime power by pursing innovative and creative

developments in basic and applied marine science and technology!

Author Index

2024 International Meeting of the Microbiological Society of Korea

292 | www.msk.or.kr

Abiola, Christiana S7-1

Abraham, Soman D009

Acme, Afrin Jahan B134

Ahmad, Shakra G035

Ahn, Chi-Yong A011

Ahn, Heejin G040

Ahn, Jae hyun D042

Ahn, Jae-Hyung B075, B077,

B109, B111

Ahn, Jihee D051, D059

Ahn, JinWoo B073

Ahn, Jinwoo B105

Ahn, Junmo B099

Ahn, Seohyun B034

Ahn, Sung Yoon G002

Aiba, Yoshifumi G021

Alcantara Jr., Alfredo E022

Ali, Mohammed D074

Allam, Fatma Ali D054

Alspaugh, J. Andrew S3-3

An, Jeongshin H025

An, Jieun D077

An, Jongbin B117

An, Seojeong D058

An, Seokyoung H033

An, Seong Ho S21-2, F017, F018

An, Si-Hyun B075, B077

An, Sihyun B109

An, Sohyeon H025

An, Woo Man G037

An, Yang Joon D055

An, Yewon D035, D037, D038

An, Young Jun E034

An, Yu-Jeong C010

Andargie, Yohannes Ebabuye

GS3-9

Angulmaduwa, Sacheera D005,

F037, F038, F039

Aprilliza AM, Mozart Nuzul B103

Awala, Samuel Imisi S7-1

Aziz, Israr B118

Bae, Da-Woon S16-4, GS3-11,

E034, E036

Bae, Gahee D057

Bae, Hee-Won S4-2, GS1-7,

F030, F031

Bae, Hyunjin C071

Bae, Il Kwon H048

Bae, Jin-Woo A019, A032, A043,

B100, C001, C014, C031,

C080, D073, D075, D076, H050,

S1-4, GS1-5, GS1-11

Bae, Sangmyeong F028

Bae, Seohyeon A062

Bae, Seung Seob G018

Bae, Sojung H042

Bae, Yurim B048

Baek, Ju Hye B059, B060

Baek, Nuga B067

Baek, Seong Min LS3

Baek, Seongeun D071

Baek, Seungwoo H008

Baek, Youl-Chang H035

Bahn, Yong-Sun S3-3, F011

Bai, Jaewoo S4-5

Baik, Keun Sik B103

Bak, Gyeryeong B065, C032

Bak, Hyun Ryul H045

Bak, Jeong Eun C037

Ban, Byeong Cheol G015

Bang, Iel Soo E002, G002

Bang, Jiwon B107, F026, F027

Bang, Sein D051, D057

Bang, Yeji D053

Bang, Ye-Ji S17-3, E007, G001

Bashizi, Tino B143, C058

Bayburt, Hülya S3-5, B064, B089

Been, Kyoung-Kuk G012

Beis, Konstantinos S16-1

Bekele, Tigist C002

Beom, Jin young D042

Berdos, Janine I. B103, B104

Boldbataar, Chinchuluun B113

Bong, Seong-Hun H049

Byeon, Heewon S20-1

Byun, Ha Yeon C028

Byun, Ji-Won E009

Byun, Jongwon C013

Byun, Yejee H006

Cai, Jian-Na H007

Cha, Chang-Jun A009, A025,

A055, B039, B040, B041

Cha, In-Tae B017, B018

Cha, Jeong-Heon D005, F037,

F038, F039

Cha, Jimin S1-3

Cha, Myeong-Gi G026

Cha, So-Jeong D033

Cha, Sung-Kyung B130

Cha, Sun-Shin S16-3, S16-4,

GS3-11, E018, E034, E036

Cha, Yehyeon GS1-10, B114, B117

Chae, Hansong H004

Chae, Jong Pyo C050, C052, D030

Chae, Jong-Chan B081,

B087, B091

Chae, Myeongju D003, D081

Chae, Yeongjae S14-2, GS1-2,

G008, H013

Chalita, Mauricio B067

Chang, JuOae S2-5, GS2-4, D002

Chang, Shu-Jung S2-2

Chang, Soojeong S8-5

Chang, Sun-Sik H035

Chang, Yoon Hwan D072

Chang, Yun C. S13-3

Chau, Le Tran Tien B039

Chen, Lei B027, B062

Cheon, Young Jin B072

Chhetri, Geeta A006, A007, A008

Chi, Won-Jae E031

Chin, BumSik D040, F012

Chin, Young-Do D004

Cho, Byung-Kwan S6-4, S6-5, F001

Cho, Donghyun A041, A042

Cho, Dong-Wan B006

Cho, Hae Jin A038, A039

Cho, He-Jin A054, F024

Cho, Heui Kyoung F015, H033

Cho, Heuikyoung H034

Cho, Hongbaek GS2-8

Cho, Hyun Woo H017

Cho, Hyun-Soo S12-5

Cho, Iljee C054, C066

Cho, Insook B002

Cho, Ja Young A023

Cho, Jang-Cheon AL, GS4-3,

A058, B014, B015,

B016, B036, B095

Author Index

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Cho, Jin Ah B032

Cho, Jinsik H001

Cho, Junho GS3-8

Cho, Junhun D019

Cho, Kwangmoon C047

Cho, Min-ji H014

Cho, Minseo A059, A060,

B115, B116

Cho, Nam-Hyuk S12-5, D072

Cho, Seok-Cheol E031, E033

Cho, So-Min E035

Cho, Suyeon H041

Cho, Tae Oh B048

Cho, Yeasol E004

Cho, Yong-Bin S3-6

Cho, Yong-Joon S21-1, B107,

D005, F027

Cho, Yong-joon F026

Cho, Yongmin D018

Cho, YongUk C043

Cho, Yoon Soo GS1-4, C081

Cho, Yoonhee A029

Cho, You-Hee S4-2, GS1-7,

F030, F031

Cho, Youngjin GS3-1

Choe, Donghui F001

Choe, Seon-Bong B055

Choe, Sun-Bong C035, C038

Choi, Ahyoung A022, A023, A024

Choi, Bo Gyoung E029, G005

Choi, Boram D033

Choi, Bo-Yun B068, B108

Choi, Byeong Jun S3-5, A037,

B064, B089

Choi, Dae Gyu B058

Choi, Donghyeok H025

Choi, Dongjin H008

Choi, Eui-Hwan S3-4

Choi, Eun-Jeong B038

Choi, Eun-Jung S20-3

Choi, Eunmi C054, C066

Choi, Eunsil E004

Choi, Geon B008, B072

Choi, Gi-Sub S18-4

Choi, Ha Kyoung H024

Choi, Haeun D012

Choi, HaeWoong D009

Choi, Han Suk B075, B077,

B109, B111

Choi, Heeyun S9-2

Choi, Huiwon B124

Choi, Hyeiweon C070

Choi, Hye-Jeong G046

Choi, HyeLim GS2-12, GS3-10

Choi, Hyeongju G037

Choi, Hyorim A017, A018, A044

Choi, Hyungjun D030

Choi, Jae-Hyeok D004

Choi, Jaeyoung S13-1,

F023, H036

Choi, Jee-Won A032, C001, C014

Choi, Ji Su H011

Choi, Ji-Hyun F040

Choi, Jinho S17-4

Choi, Jiwoo D069

Choi, Junhyeok D018

Choi, Jy Young B132

Choi, Kang Seok C069

Choi, Kihyuck S9-2

Choi, Kyung-Min A048

Choi, MoonHo G051

Choi, Myeung Seok H008

Choi, Sang Ho D007, D008, D023

Choi, Seo Hui A037

Choi, Seojin H026

Choi, Seol-Hwa G048

Choi, SeungHee A016

Choi, Seung-Hyon GS2-9, C079

Choi, Shin-Yae S4-2, GS1-7,

F030, F031

Choi, Si-Sun S6-1

Choi, Soomin S21-5

Choi, Soyoung H035

Choi, Sumin G011

Choi, Suwan D051, D057

Choi, Tae-Jin D062, G040

Choi, Ui-Lim B043

Choi, Umji S16-5, E006

Choi, Ye Seul GS2-11, C083

Choi, Yejin S14-2, GS1-2,

G008, H013

Choi, Yelim G010, G014

Choi, Yelin C021

Choi, YeongSeon D010

Choi, Yeon-ju D059

Choi, Yo Han S9-3

Choi, Yoon-Jung A010, C003, D050

Choi, Young Im E033

Choi, Young Sik A043

Choi, Yu Jin C016, H028,

H030, H031

Choi, Yun Ji C060

Choi, Yunchan S16-3

Choi, Yunhee A017, A018, A035

Chong, Gun Oh GS2-11, C083

Chu, Eunmi D080

Chu, Jiyon F029, F036

Chun, Jang Woo G045

Chun, Jongsik B067

Chun, Julan H017

Chun, Se won A048, B056, B057

Chun, Se Won B042

Chung, Young-Uk A012

Chung, Dong Hwa C025

Chung, Han Young S17-2

Chung, Hye Kyung D027

Chung, Jeong Min GS2-8

Chung, Joon-hui B075, B077,

B109, B111

Chung, Sun Ju GS1-5

Chung, Won-Seok C008

Chung, Yeun-Jun F021, F029, F036

Cui, Longzhu S4-3, G021

Dahal, Ram Hari A010

David, Sophia S16-1

Delgado, Monica Paola Albuja B133

Dhia, Khanza Syahira B103

Dhond, Vaibhavi C002

Do, Hyerim B080

Dong, Ke A034, A040, A049,

B027, B028, B062

Doo, Hyunok S14-2, GS1-2,

G008, H013

Ebabuye, Andargie Yohannes C034

Ekpenyong, Joy A031

Ekpenyong, Joy Edet C024

Elgabbar, Mohammed Abdo F041

Eom, Mi Kyung A069

Eyun, Seong-Il C043, C056, H043

2024 International Meeting of the Microbiological Society of Korea

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Faisal, Kudsia B085

Fentie, Eskindir Getachew GS3-3

Franke, Gad S16-1

Fujita, Takashi S12-1

Furuta, Tadaomi E001

Ganbat, Dariimaa GS1-3, A057,

E029, G056

Ganbat, Sondor GS1-3, A057,

E029, E031, E033, G056

Garridor-Amador, Paloma B035

Ghatge, Sunil E037

Gho, YuJeong S19-2

Gim, Do Hun D075

Gim, Yeonji B002

Glass, John PL3

Go, Boram C019, H016

Go, Eunbyeul D048

Go, Gyungnam C077

Go, Seong-Ryung S11-2

Goh, Jae Duk A030, A033

Goh, Jaeduk A022, A023, A024

Gong, doHun D010

Goo, Beom Jin A019

Goo, Beom-Jin H050

Gook, Do-Hun C017, C020

Gu, Min Jeong C052, D030

Gwak, Jae Yeon D054

Gwak, Joo-Han S7-1

Gwak, WonSeok D039, G016

Gwak, Ye Jin B053, B094

Gyeong, Hyeryeon B096

Ha, Neul I H028, H030, H031

Ha, Seung Hee B070

Ha, Sun-Yong GS4-3

Ha, Yonkyoung D057

Hahn, Ji-Sook GS3-11

Hahn, Yoonsoo B060, H008

Haller, Dirk D063

Ham, Seung Hee C028

Ham, Soojeong E018

Ham, Young-min C019

Ham, Young-Min H016

Han, Byeong-Hak A017, A018

Han, Dasom H023

Han, Dong Min B058

Han, Douk Ju B113

Han, Dukki B026

Han, Gyeong Hak B076, B086

Han, Hee-Jin F023

Han, Hyung Soo GS2-11, C083

Han, Inyoung D004

Han, Jee Eun D031

Han, Jeong Eun GS1-11, D073

Han, Jeong Moo D053

Han, Jeong-Eun C080

Han, JeongEun H050

Han, Jisoo B016

Han, Kap-Hoon F041, G043

Han, Sang-Uk S10-1

Han, Sang-Wook D018

Han, Seong Eun C068

Han, Seung Min B123

Han, Seung-Min B139

Han, Seungmok E009

Han, Sia GS4-2

Han, Sol G017

Han, Song-Ih A064, B128,

B131, D079

Han, Soo Hyun B125, B126, H048

Han, Su Hyeon S9-1, B079

Han, Yeongcheol GS4-3, GS4-9,

B024

Han, Yoobin D018

Han, You-Kyoung H019

Hathurusinghe, Sandamali

Harshani Kumari B092

Heo, A Yeong A036, B052, C053

Heo, Eunseo C037

Heo, Hokwan B051

Heo, HyeSook D020

Heo, Jae Young H004

Heo, Jun GS3-10, A017,

A018, A035, A044

Heo, Seoyoung G030

Her, Moon D003, D022, D081

Higashide, Mika T S3-4

Higashitani, Atsushi E022

Ho, Xuan Bach H010

Hoang, Vu Hanh Dung C026

Holzapfel, Wilhelm D063

Hong, Giwook B099

Hong, Gyeochang C036

Hong, Gyuri G037

Hong, Hye Jin G045, G047

Hong, Jieun H025

Hong, Jin-Kyung S20-2, B098

Hong, Moon-Gi C008

Hong, Seo-Young D065

Hong, Seung-Beom A017, A018

Hong, Soogil S3-4

Hong, Subin GS2-4, D001

Hong, Sung Wook S5-3

Hong, Sung-Chul F023

Hong, Yeonju C055

Hoshi, Naomi E001

Humaira, Zalfa A041, A042

Hur, Ho-Gil C026

Hur, Hoon S10-1

Hur, Hor-Gil B023, E037

Hur, Hor-gil B085

Hur, Jae-Seoun GS1-10,

B006, B007

Hur, Kyu-Hwa F035

Hur, Moonsuk B099

Hwang, Buyng Su H011

Hwang, Cheol-Sang S21-3

Hwang, Chung Yeon GS4-9,

B110, B132

Hwang, Dae II H024

Hwang, Eun Ji D055

Hwang, Geum-Sook D053

Hwang, Gukhwa B099

Hwang, Heejoo C048, C051

Hwang, HeeJoo GS2-5, C084

Hwang, Heeyoun S3-3

Hwang, Hee-Young B004

Hwang, Hyeon-Ji D032

Hwang, Hyeseong F014

Hwang, Hyesun H006

Hwang, Hyun-Ju C004, G018

Hwang, Jeong Ho B141

Hwang, JeongYun D010

Hwang, Ji Yun A065

Hwang, Jihwan E004

Hwang, Jin C019, H016

Hwang, Jiseon H021

Hwang, Seo Hyun C068

Hwang, Seong Bo G003

Author Index

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Hwang, Si-hyeon B127

Hwang, So-Yeong G036

Hwang, Yoojung F021, F035

Hwang, Yujin A024

Hwang, Yuna G006, G009

Hwang, Yun-Ho D056

Hyeon, Hana S3-1, F003

Hyun, Ho Bong C019, H016

Hyun, Jaekyung S2-3

Hyun, Jung Mi G032, G033

Hyun, Youbong B124

Hyun, Yujin C048, F010

Ignatius, Lintang Satyawan H043

Ihara, Kunio E001

Im, Jong Hee B122

Im, Ju-Hee B001

Im, Seung-bin H028, H031

Im, Wan-Taek A052

In, Su-A B031, B032, B033

Ishimaru, Yasuhiro E001

Itoh, Takashi S7-3

Jang, Bora B017, B018

Jang, Bo-Ram S3-2, S16-4, E035

Jang, Byung Chun A013, D024

Jang, Eun Bi C019, H016

Jang, Hyochan C022, C024,

E022, G011

Jang, Hyun Dong H023

Jang, Hyun-Jun B055, C038, H022

Jang, II D052, F009, F013

Jang, Jaehyuk C062

Jang, Jeong Uk B104

Jang, Jeonghwa C050

Jang, Jeonghwan B034, B046,

B073, B105

Jang, Jichan D006

Jang, Jihun G042, H051

Jang, Juhui GS2-6

Jang, Kyoungwoo LS2

Jang, Min Suk GS4-8

Jang, Seokhun D012

Jang, SuJin D062

Jang, Sumin A022, A024

Jang, Tae Yeong H027

Jang, Taeyoung F028

Jang, Woojin B102

Jang, Yeongseon E014, F006, F016

Jang, Yeontae S2-3

Jang, Yo Han E029, E033

Jang, Yuhyun S3-1, F003

Jang, Yu-Jeong H003

Jang, Yunhyeok GS1-10,

B114, B117

Jayan, Jaincy N A034, A049,

G022, G028

Jeon, Chae Ran D041

Jeon, Chae-Ran D054

Jeon, Chan Woo E005, E023

Jeon, Che Ok S3-5, S10-2,

A037, B058, B059, B060,

B064, B089, C031, C043

Jeon, Hosung S13-4

Jeon, Hye-Rim G043

Jeon, Jae Gyu H005

Jeon, Jae-Gyu H007

Jeon, Ji Hyang D056

Jeon, Jin Pyeong F015

Jeon, Kyeongseok S12-5, D072

Jeon, Se Young GS2-11, C083

Jeon, Taeck Joong E024,

E025, E026

Jeon, Wook-Jong GS2-8

Jeon, Yihyun A026, A027, A028,

A038, A039, A062

Jeon, Yong-Min G029

Jeon, Yoon Kyung D072

Jeon, Yuna B111

Jeong, Ah Rah C015

Jeong, Bo-Gyeong GS3-11, E036

Jeong, Chaerin A046, A056

Jeong, ChaeRin A047

Jeong, Chan-Seok H012

Jeong, Da Eun G020, G023

Jeong, Dae Won H011

Jeong, Da-eun D016

Jeong, Eu Gene G032

Jeong, Eugene G033

Jeong, Eunji B120

Jeong, HaeRyn C072

Jeong, Hee-Gyeong H028,

H030, H031

Jeong, Hye Sook D039, G016

Jeong, Hyewon G002

Jeong, In-Chul C001, D073

Jeong, Jaehoon H006

Jeong, Jaewoong S20-1

Jeong, Jeongyun E020

Jeong, Ji Eun B119

Jeong, Ji Hye A038

Jeong, Jihye A039

Jeong, JinGyeong B135

Jeong, Jiwon B106

Jeong, Jiyeon D003, D081

Jeong, Jong Sik G015

Jeong, Juwon E020, E032

Jeong, Kwi Wan G032

Jeong, Kwiwan G033

Jeong, Minsoo GS2-11,

B143, C045, C083

Jeong, Sa Rang B127

Jeong, Sang-Wook H028,

H030, H031

Jeong, Seok Hoon B081

Jeong, Seul-A H035

Jeong, Song Hee S4-4, GS4-1,

GS4-6, G027, G053, G055

Jeong, So-Yeon B003

Jeong, Su On GS4-10, G052

Jeong, Su-An G043

Jeong, Su-Won GS1-11,

C080, D073

Jeong, Taeho D012

Jeong, Uk Jin D074

Jeong, Yeonghwan C053

Jeong, Yong Tae H011

Jeong, Yoon Chae G024

Jeong, Yoonchae E020

Jeong, Yujeong G013

Jeong, YunSeok B100

Jeong, Yun-Seok H050

Ji, Chang Jun E017

Ji, Joon-Hwan S18-4

Ji, Sangwoo B006

Ji, Yosep D063

Jia, Baolei S10-2

Jin, Chunzhi A067, A068, B136

Jin, Do Young C077

Jin, Do young C078

Jin, Hohyun H001

Jin, Hyein C004

Jin, Joo Hyun A038, A039

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Jin, Long A068

Jin, Sangrak S6-4

Jin, Seong-Woo H028, H030, H031

Jin, So-Yeon GS1-8, C021

Jin, Yong Ju H020

Jo, Areum D059

Jo, Aruna E014, F006, F016

Jo, Eun-Kyeong D006

Jo, Hana GS3-12, D014

Jo, Hee Eun C005, C006

Jo, Hyunyoung B095

Jo, Jae-won B047

Jo, Jong Won A038, A039

Jo, Seobeen C074, C075

Jo, Seona F013

Jo, Seong-Jun B101

Jo, Sohyeon G040

Jo, Sumin B045

Jo, Sungyang GS1-5

Jo, Yong Ho H015

Joe, Hae In A032

Joe, Hae-In H050

Joh, Kiseong A026, A062

Joo, Jeong Hwan S3-4

Joo, Jung-Hun A052

Joo, Minju S3-1, F003

Joung, Jae Yeon C021

Joung, Yochan A026, A062

Ju, Min Kyung G047

Ju, Sun Min H024

Jun, Sang-Cheol F041, G043

Jung, Chung Ryul A036

Jung, DaGyeong C055

Jung, Da-Ryung GS1-4, GS2-11,

C081, C083

Jung, DoYoun G040

Jung, Geunsoo GS2-10

Jung, Hae-Chang G005

Jung, Harry F015

Jung, Hee C002

Jung, Hee Young H005

Jung, Hee-Young H007

Jung, Ho Sang H029

Jung, Hyein G013

Jung, Hye-Jin B130

Jung, Hyeon Ho G042

Jung, Hye-Ri D003, D081

Jung, In Kyung S8-5

Jung, Ji hye B043

Jung, Ji Young A023, A024,

A030, A033, B141

Jung, Ji young B013

Jung, Jinyoung B110

Jung, Jiwoon E017

Jung, Jong-Hyun F011

Jung, Ju-Hyeong F023

Jung, Kwang-Woo F011

Jung, Man-Young B118, B119,

B122, C060

Jung, Seung-Hyun F021, F029,

F035, F036

Jung, Seung-Woon G030

Jung, Soojung B013

Jung, Sungju B012, B037

Jung, Ye-Eun E034, E036

Jung, Yein D077

Jung, YeonGyun GS1-4, C081

Jung, Yeon-Ju E018

Jung, Yonghee A008

Jung, Yoon-Hee G018

Jung, Young Hoon F023

Jung, Yu Jung F028

Jung, Yun-Ha B001

Ka, Kang-Hyeon E014, F006, F016

Kahng, Hyung-Yeel B005

Kamada, Nobuhiko PL2

Kang, Bo Ra D048

Kang, Bo Ram B138

Kang, Boram D057

Kang, Byung Hak D034, D035,

D036, D037, D038

Kang, Chang Hee H011

Kang, Chang-Yuil S8-5

Kang, Dongsu C077

Kang, Dong-Wan D004

Kang, Dukjin S1-1

Kang, Eun-Jung S1-1

Kang, Eunsol D004

Kang, Hae Ji H018

Kang, Heeyoung A026, A027,

A028, A062, B044

Kang, Hojeong B082

Kang, Hye Kyeong A024

Kang, Hye-Kyeong A030

Kang, Hyekyeong A033

Kang, Hyun Ah S3-3, S3-4,

C027, C043, C049, C056, D011

Kang, Hyun-ah H043

Kang, Ilnam A058, B014, B015,

B016, B036, B095

Kang, JiHye D020

Kang, Jin-Han S18-4

Kang, Juhyun F022

Kang, Jun Won B054

Kang, Jun-Gu A012

Kang, Jun-gu D016

Kang, Juyoun S14-2, GS1-2,

G008, H013

Kang, Mijeong H029

Kang, Min Joo B076, B086

Kang, Minchung E015

Kang, Minhee D012

Kang, Min-Hee D032

Kang, Min-Ho S3-3

Kang, Minji D033

Kang, Min-Ju B118

Kang, Minseok A040, G028

Kang, Min-Seong D017

Kang, Min-Su D003, D081

Kang, Ryuk Seok G015

Kang, Se Won S9-3

Kang, Sebin E035

Kang, Seon D066

Kang, Seon-Hee B130

Kang, Seunghyun G031

Kang, Sung Gyun S16-4,

F025, G005

Kang, Woorim D019

Kang, Ye-Jin A054, G046

Kang, Yue Jai F023

Kang, Yunsoo H025

Kartal, Boran B035

Kathiresan, Akila B062, B063

Kato, Shigo S7-3

Kerfahi, Dorsaf B028

Keum, Gi Beom S14-2, GS1-2,

G008, H013

Keum, Hye Lim B061

Ki, Hyeong-Jun S4-2, F030

Kim, Minseok B095

Kim, Aeryun D005

Kim, Ah-Ram G039

Kim, Beom-Tae G041

Kim, Bitnara S15-4, GS2-7

Author Index

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Kim, Bong-Soo B021, B029,

B047, C009, C010

Kim, Bong-soo B127

Kim, Bu-Min C076

Kim, Byoung Sik S16-4, S21-4,

GS2-1, D083

Kim, Byoung-Sik C023

Kim, Byung-Yong S19-4

Kim, Chaehyun C077

Kim, Chae-yul D039, G016

Kim, Changmu A060

Kim, Chang-Ung D046

Kim, Cho Hee E005, E023

Kim, Da Sol D078

Kim, Da Som A064

Kim, Da som B128

Kim, DaChan A045, A047

Kim, Da-Chan A061

Kim, Dae-Hyuk S9-4, GS3-4,

C035, C075, G041, G054

Kim, DaeKyo D010

Kim, Daeun C056

Kim, Da-Eun D033

Kim, Dae-Wi B041, B101,

B121, F032, F033

Kim, Dae-Woon C040

Kim, Dahyeon E024, E026

Kim, dasom B131

Kim, Dasom D079

Kim, Da-Yeon B075, B077, B109

Kim, Deok Ryun S18-1

Kim, Do Yeon C031

Kim, Do Yi E005

Kim, Dockyu B011

Kim, Dohak C008

Kim, Dohyeon G017, G034, G035

Kim, Do-Hyun D044

Kim, Dokeun D056, D058

Kim, Dokyun B081

Kim, Dong Uk D015

Kim, Dong-Ho H029

Kim, Donghwee E009

Kim, Dongjin B048

Kim, Dong-Min D072

Kim, Dong-Uk H028, H030, H031

Kim, Dong-Wan B001

Kim, DongYeol C042

Kim, Dongyeol D063

Kim, Dongyeop H002, H005,

H007, H009, H010

Kim, Do-Yeon D073

Kim, Doyi E023

Kim, Do-Yun A013

Kim, Eui-Seong GS4-11, D066

Kim, Eun Bae D043, D061,

G025, G044, H026, H038

Kim, Eun Sun D048

Kim, Eunbin G006, G009

Kim, Eungseok H015

Kim, Eung-Soo S6-1

Kim, Eunji GS3-11, C054, C066

Kim, Eunjung C042, C044, C046

Kim, EunSoo C051

Kim, Geon C011

Kim, Geonmin G041

Kim, Geon-Min G054

Kim, Geon-min GS3-4

Kim, Geun-Joong G012,

G048, G049

Kim, Gowon D077

kim, Gyeonghwa D057

Kim, Gyeongmin GS3-11, D084

Kim, Gyeong-min GS4-5

Kim, Gyungcheon C061, H051

Kim, Gyun-Hee B001

Kim, Haeun C033, C044, C046

Kim, Hae-Un D065

Kim, Haneul A026, A062

Kim, Han-Shin B112, D065

Kim, Hanul C012

Kim, Hanwool C052

Kim, Haram S14-2, GS1-2,

G008, H013

Kim, Hea Joon C025, F004

Kim, Hong Ki D034, D036

Kim, Hong Sik E005, E023

Kim, Hoyeon C042, C046

Kim, Hun S18-1

Kim, Hyang su D040, F012

Kim, Hyangmi A023, A024

Kim, Hye Ji H033

Kim, Hye lin D015

Kim, Hye su D040

Kim, Hye-Ji F015

Kim, Hyeji H034

Kim, Hye-Jin G046

Kim, Hyeon Jeung D015

Kim, Hyeon Jin B068

Kim, Hyeonjeung B002

Kim, Hyeon-Ji F034

Kim, HyeonJin B108

Kim, Hyeonjung D051

Kim, Hyeon-jung H044

Kim, Hyeran B097

Kim, Hyeri C036, C047

Kim, Hyeun Bum S9-3, S14-2,

GS1-2, G008, G008, H013

Kim, Hyo Jin C025, D028, F004

Kim, Hyojin B112

Kim, Hyomin G010

Kim, Hyo-Min G014

Kim, Hyun Ju A023, A024,

G027, G038, G052

Kim, Hyun Ju GS4-10

Kim, Hyun Sik S1-4, H050

Kim, Hyungbin G050

Kim, Hyung-Seop B062, B063

Kim, Hyunhee E016, E018, E020

Kim, Hyunjoong E025

Kim, Hyun-Ju E034

Kim, Ikwhan GS1-4, C081

Kim, Inhyup A001, A002, A003,

A004, A005, A006, A007, A008

Kim, Inseo GS2-4, D002

Kim, Inseon B010

Kim, Jae Hyung C064

Kim, Jae-dong D059

Kim, Jae-jin A059, B115

Kim, Jae-Jin A060, B116

Kim, Jae-Kyeom D022

Kim, Jae-Kyung S9-3, H014

Kim, Jae-Myung F040

Kim, Jangho D051, D057

Kim, Jedong D057

Kim, Jeong Ho H011

Kim, Jeong Min S3-5, A037,

B064, B089

Kim, Jeong-A B137

Kim, Jeongyun G008

Kim, Ji Hyun F019

Kim, Ji Hyung D026, D031

Kim, Ji Seon GS2-2, A029, B045

Kim, Jieun C052

Kim, Jihee H018, H044

Kim, Ji-Hoon GS2-4, D001

Kim, Jihun C074

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Kim, Jihyeon D001

Kim, Jihyun F. B049

Kim, Jihyun G004

Kim, Jin D070

Kim, Jin Hwan S14-3

Kim, Jin Ju B068, B108

Kim, Jin-Cheol A011

Kim, JinHo A047

Kim, Jin-Ho A056, A061

Kim, Jinhyun B141

Kim, Jin-Il A056, A061

Kim, Jinwoo GS2-10

Kim, Ji-Soo B001

Kim, Ji-Sun GS2-9, A069, C079

Kim, Jiwon A036, B052, C053

Kim, Jong Heon S8-5

Kim, Jong-Geol S20-3, B038, F007

Kim, Joo Yeol B087, B091

Kim, Joon Ki D034, D036

Kim, Joowan D072

Kim, Jooyoung S18-3

Kim, Jueun S21-1

Kim, Juhee B107, F026, F027

Kim, Juhyun F022

Kim, Jun Yup F015

Kim, Jung-Eun S13-4

Kim, Jung-Mi C074, C075

Kim, Jungmin A010, C003, D050

Kim, Jungwoong C050

Kim, Jungyeon S2-4

Kim, Jungyeop S3-1, F003

Kim, Jun-Seob C077, C078

Kim, Kahyun E009

Kim, Kangsan S6-5

Kim, Keu hoi D042

Kim, Keun Pil S3-4

Kim, Ki Beom H027, H029

Kim, Ki Hyun H017

Kim, Kil Yong C068

Kim, Kiseung S3-3, D011

Kim, Kitae G004

Kim, Kwangsoo D053

Kim, Kwang-sun GS4-5, D084

Kim, Kyeonga H006

Kim, Kyoung Su B123, H045

Kim, Kyoung-Dong S21-2, F017,

F018, F019, F020

Kim, Kyu-Hyun A054

Kim, Kyung-A D017

Kim, Kyunghyun A011

Kim, Kyung-Je H028, H030, H031

Kim, Kyung-yun S19-3

Kim, Kyuseok B050

Kim, Li-Ha C021

Kim, Mee-Jeoung B004

Kim, Mi Hee B043

Kim, Min Gyeol C016

Kim, Min Jeong F008

Kim, Min Jin H011

Kim, Min Ju C028, C043

Kim, Min Kyeong E031

Kim, Min Seok B012

Kim, Min Sung B138

Kim, Mina E002

Kim, Mincheol B011, B013, B141

Kim, Minjae D057

Kim, Minjeong A058, B010

Kim, Min-Ji GS2-5, C084, F010

Kim, Minji F014

Kim, Min-Ju F031

Kim, Min-Jun E014, F006, F016

Kim, Minkyung GS4-9, B024,

C022, C024

Kim, Min-Kyung D040, F012

Kim, Min-Seo F037, F039

Kim, Minseo F038

Kim, Minseok B012, B037

Kim, MinSeok B037

Kim, Minsik C063, C064, C065,

C067, C069, C070

Kim, Min-Soo B031, B032, B033

Kim, Min-young D059

Kim, Mirae B036

Kim, Mirim D046

Kim, Miryung A025, B039

Kim, Miso C033

Kim, Myeong-A D005,

F037, F038, F039

Kim, Myeongjae B102

Kim, Myeong-Yeon GS3-11

Kim, Myong Ki E031

Kim, Myoung Dong G047, G026,

G036, G045

Kim, Myung-Hoo G015

Kim, Myungsuk C002

Kim, Na Yun C025, D028

Kim, Naeun C057

Kim, Nam Kyu A039

Kim, Namhee C006

Kim, Nam-Hoon F032

Kim, Namwoo H032

Kim, Nam-Young H035

Kim, Na-Ri S20-3, B038

Kim, Narin E030

Kim, Na-Yeon H035

Kim, Nayeong F002

Kim, Ok-Sun GS4-3, GS4-9,

B019, B022, B024, B069

Kim, On-Yu B088

Kim, Pureun S15-4

Kim, Pyoung Il B088

Kim, Pyung Il G037

Kim, Ryeong Hui C041

Kim, Sae Hun H033

Kim, Sang Ho H027

Kim, Sangbum A018

Kim, Sang-Bum B111

Kim, Sang-Gyu B121, F033

Kim, Sangha E019

Kim, Sa-Ra B130

Kim, Se Joung B129

Kim, Se Kye B113, D067

Kim, Se-Been E036

Kim, Seok Jin D019

Kim, Seon Ho B103, B104

Kim, Seon Yeop G044

Kim, Seong Yeob F002

Kim, Seong-Bo S6-6, GS1-3,

A057, G056

Kim, Seong-Hyeon E013

Kim, Seongik F014

Kim, Seongok GS2-6

Kim, Seongryong D056, G007

Kim, Seongwook B122

Kim, Seon-Ho B097

kim, SeonHwa D010

Kim, Seoyeon C023

Kim, Seo-Yeon D056

Kim, Seung Bum A045, A046,

A047, A056, A061, A063

Kim, Seunghwan A017, A018, A044

Kim, Seunghyun A013, D024

Kim, Seung-Jun G026

Kim, Seungmi S2-3

Kim, Seung-Tae A025

Kim, Sheena S14-2, GS1-2,

G008, H013

Author Index

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Kim, Shukho A010, C003,

D050, F002

Kim, Si Cheol B053

Kim, Sieun S13-4, H019, H038

Kim, So-Jeong S20-3

Kim, Soo Bin S20-2, A053, B071

Kim, Soo-Ki A065, A066, F034

Kim, Soo-Min D058

Kim, Soonok F007

Kim, Soyeon GS4-3, B083

Kim, So-young D059

Kim, Su Yeon D034, D036,

D037, D038, D062

Kim, Subin C005, C006

Kim, Suhyun GS4-3, A058,

B014, B015, B016

Kim, Su-Hyun G040

Kim, Sukho A020

Kim, Sumin B014

Kim, Sung Hyun A029

Kim, Sung Min F008

Kim, Sung Tae GS1-3, G056

Kim, Sunhee D051

Kim, SuYeon D035

Kim, Su-Yeon F031

Kim, Tae Gwan B003

Kim, Tae Gyun F009

Kim, Tae Wan S8-5, G018

Kim, Tae Young G015, G020,

G023, H024

Kim, Tae-Gyun S1-3

Kim, Tae-Hwan D013

Kim, Tae-hyoun H039

Kim, Taesoo H046

Kim, Taewan C077

Kim, Taewook LS1

Kim, Taeyeong B025

Kim, Tae-Young C040

Kim, Taeyune B061

Kim, Uijin S12-5

Kim, Won Seog D019

Kim, Wonjae GS2-7

Kim, Wooseong D068, D069,

D070, D071

Kim, Yang-Min B065, C032

Kim, Yangseon S9-4, B055, C035

Kim, Ye Bin D026, D031

Kim, Yedam B020, C007

Kim, Yeoeun C044

Kim, Yeoneun H025

Kim, Yeong Ouk B067

Kim, Yeong-Chan S6-1

Kim, Yeonjae D040, F012

Kim, Yeon-Sook D072

Kim, Yeoun C046

Kim, Yesong D022

Kim, Ye-Yeong E014, F006, F016

Kim, Yiseul A017, A018,

A035, A044

Kim, Yong Joon E017

Kim, Yong Kwan B012

Kim, Yong-Hak C012, C026, E037

Kim, Yong-Hoon S1-1

Kim, Yongman S7-1

Kim, Yong-Seok A009, B041

Kim, Yongwon B141

Kim, yongwook B128

Kim, Yoonho C012

Kim, You Jin B141

Kim, You-Jin D056, D058

Kim, Young Jae D006

Kim, Young Woo C009

Kim, Young-Eui H018

Kim, Young-Eun S1-1

Kim, Younghoon S14-1

Kim, Young-Min H014

Kim, Youngrok D051, D057

Kim, Youngshin G017, G034, G035

Kim, Youngsuk B020, C007

Kim, Yu Jin B040

Kim, Yu Kyung F002

Kim, Yu Mi H049

Kim, Yu-Bin D065

Kim, Yukyung E007, G001

Kim, Yun Jae S7-4,

B106, E029, F025

Kim, Yun Ji A048,

B042, B056, B057

Kim, Yunjeong GS2-1, GS4-7, D083

Kim, Yunkyung E027

Kim, Yuri D072

Kleckner, Nancy S3-4

Ko, Do-Eun C023

Ko, Duhyun D053

Ko, Eunok H001

Ko, Haesu H035

Ko, Heejin S5-2

Ko, Hyun-Chang D078

Ko, Je Won H003

Ko, Jinsoo D033

Ko, Seo Yeon F002

Ko, So-Ra A011

Ko, Yong seok B085

Ko, Yongseok C026, E037

Ko, Young-Joon B075, B077, B111

Ko, Young-joon B109

Koh, Hong B123, H034, H045

Koh, Junseock S16-3

Koh, Young-Woo H028,

H030, H031

Kohadie, Fathrinah Binti A031,

C022 ,C024

Kong, Eunji A026

Kong, Hyun Gi S9-1, B079,

B079, G037

Koo, HeeChan E013

Koo, Tae Jeong G030

Ku, Youn-Bong C028

Kuthirakkal, Rajitha H002

Kwak, Jihye H001

Kwak, Jinok S14-2, GS1-2,

G008, H013

Kwak, Min-Kyu C055, C055

Kwak, Nakwon D033

Kwak, Su Min B081, B087, B091

Kwon, Dae Young A059,

A060, B115

Kwon, Dae-Young B116

Kwon, Hae Jun E011

Kwon, Hyesoo D002

Kwon, Joon-Gi GS4-4, GS4-7, H021

Kwon, Kae Kyoung S7-4, A014,

A015, A050, B076,

B086, B106, F025

Kwon, Ki Tae F002

Kwon, Mi-Kyung H023

Kwon, Min Young F004

Kwon, Minjeong B107, F026, F027

Kwon, Miri C065

Kwon, Mi-ye C019

Kwon, Miye H015, H016

Kwon, Muhyeok C048, C058

Kwon, Oh Hyun GS2-8

Kwon, Soo Jae E011

Kwon, Sook-Min C045, C048

Kwon, Soon-Kyeong B048,

B049, B050, G004

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Kwon, Sun Jae C036

Kwon, Sun Lul A060

Kwon, Sunhak F011

Kwon, Sunjae C047

Kwon, Sunlul A059

Kwon, Yong Min C004

Kwon, Younghee H006

Kwon-Chung, Kyung J. S13-3

Kyung, Ji-Young B001

Lai, Jing D005

Lamichhane, Ami Khanal S13-3

Le, Hong Qui B113

Le, My Tieu Ngoc G054

Le, Ngoc My Tieu GS3-4, G041

Le, Ve Van A011, B119

Lee, Gwanwoo E003

Lee, Ah-Ran A065, A066, F034

Lee, Aslan Hwanhwi A048,

B056, B057, B042

Lee, Banseok C047

Lee, Bok Luel S18-4

Lee, Boyoung B049

Lee, Chan Haeng G045

Lee, Changhan E013, E016, E018,

E019, E020, E021, E032, G024

Lee, Changkyun D063

Lee, Chang-Ro S16-5, E006

Lee, Chang-Seop GS4-11

Lee, Changwoo B073

Lee, ChangWoo B105

Lee, ChanHaeng G026, G036

Lee, Chanhee GS3-6, F005

Lee, Chan-Mi D017

Lee, Cheol Seung D041

Lee, Chi Won B003

Lee, Chul-Ho S1-1

Lee, Da Eun F002, G020, G023

Lee, Da Young F015, H033

Lee, Daeun D059

Lee, Dahye G010

Lee, Daseul A017, A018

Lee, Dayeon H032

Lee, Dayoung H034

Lee, Do Yup B123, H049

Lee, Do-Hoon B041

Lee, Dokyung C041

Lee, Donghwan C073

Lee, DongHyeon C042

Lee, Dong-Hyeon H028,

H030, H031

Lee, Donghyun C044

Lee, Dongjoo D053

Lee, Dong-Won B121

Lee, Dong-Woo S6-6, GS1-3,

A057, B021, B123, G050,

G051, G056, H034, H045

Lee, Duyoung D012

Lee, Eun Ha F023

Lee, Eun Ju D033

Lee, Eun Yeol S11-3

Lee, Eun-Jin S21-5

Lee, Eun-Seon C076

Lee, Eunyeong GS2-8

Lee, Gaeun D008, D023

Lee, Gahee G037

Lee, Gi Yong GS3-5

Lee, Gihan B098

Lee, Gun Woong B043

Lee, Gwanwoo C011

Lee, Gyeong Been G032

Lee, Gyeong In D033

Lee, Gyeongbin G033

Lee, GyuDae GS2-5, C084

Lee, Hae Ryeon D046

Lee, Haejin A003

Lee, Ha-Jung C039

Lee, Ha-Kyoung H019

Lee, Han Chul F008

Lee, Hanbyul GS4-3, B019,

B022, B024, B069

Lee, Han-Woong S12-5

Lee, Hee Ho B112

Lee, Ho Joung S4-4, GS4-1, GS4-6,

G038, G053, G055

Lee, Hojun D008, D023

Lee, Hyena C012

Lee, Hyeon Seop C022, C024

Lee, Hyeon seop G011

Lee, Hyeon Yeong E005, E023

Lee, Hyeon-jeong H039

Lee, Hyeonjun G025

Lee, Hye-Rin D003, D081

Lee, Hye-Seong C023

Lee, Hyo Jung B053, B099

Lee, HyoJung B094

Lee, Hyo-Jung F015

Lee, Hyoungseok B124

Lee, Hyowon C013

Lee, Hyukjin S18-2, G006, G007,

G009, G010, G013, G014, G019

Lee, Hyun Jung B097

Lee, Hyun Sook S16-4, E011,

F025, G005

Lee, Hyunah H032

Lee, Hyung-Gwan A067, A068,

B136

Lee, Hyunji A001, A002, A003,

A004, A005, A006, A007, G031

Lee, Hyun-kyung B001

Lee, Imchang C010

Lee, Jae Hoon S12-5

Lee, Jae Kyeong B060

Lee, Jae Min S21-2, F020

Lee, Jaehoon C036, C047

Lee, Jaehyun B082

Lee, Jaewon C047

Lee, Jaeyoung C023

Lee, Jae-Yun S1-4, GS1-5,

C031, D019, H050

Lee, Jangheon B010

Lee, Je Chul F002

Lee, Jeong Yoon GS3-6, F005

Lee, Jeong-Tae B065, C032

Lee, Jeongwook D018

Lee, Jeonmin

Lee, Ji Hee D037

Lee, Ji Hyen B090

Lee, Ji Hyeon B125, B126, H048

Lee, Ji Yoon D021

Lee, Jihyun B102, B129

Lee, Jin I. E022

Lee, Jina A048, B042, B056, B057

Lee, Jinho G008

Lee, Jin-Jae B021, B029,

B047, B127

Lee, Jinkyeong F029

Lee, Jinkyung F035

Lee, Ji-Yeon G046

Lee, Jiyoung A041, A042

Lee, Jong Seok F007

Lee, Jong-Du C019, H016

Lee, Jong-Seok B099

Lee, Joon Suk E017

Author Index

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Lee, Joon-Hee D032, D041,

D054, E012

Lee, Joungmin E011

Lee, Ju Huck S9-3, GS2-9,

C037, C079

Lee, Ju Yong B074

Lee, Jueun D053

Lee, Ju-Hoon S5-2, S9-3, S14-2,

GS1-6, GS1-8, GS4-4, GS4-7,

C021, H014, H021

Lee, Jun B132

Lee, Jun Bong D045

Lee, Jun Won A029, F025

Lee, June-Woo D039, G016

Lee, June-Yong D080

Lee, June-Young C031, D073

Lee, Jung Hun F032, F033

Lee, Jung Hyen S16-4

Lee, Jungeun B124

Lee, Jung-Hwan S5-1

Lee, Jung-Hyun S7-4, F025

Lee, Jun-gi D049

Lee, Jung-Shin S21-1

Lee, Jungsoo D078

Lee, Jung-Sook GS2-9, A069,

B030, C079

Lee, Kang Soo S15-2

Lee, Kangseok S3-1, F003

Lee, Keun-Woo D027

Lee, Kihyun B041

Lee, Kyu Hyun A052

Lee, Kyu-Chan B069

Lee, Kyu-Ho S3-2, S16-4, E035

Lee, Kyung Jin B099

Lee, Kyung-Jo S3-2

Lee, Kyung-Soo D046

Lee, Kyung-Tae S13-2, GS4-11,

D066

Lee, Min Ju H020

Lee, Min Seo A050

Lee, Min Young H017

Lee, Minho B051, E009

Lee, Min-Su S3-4

Lee, Moo-Seung D046

Lee, Munhoe S6-6

Lee, Mun-Jung H020

Lee, Nahyun S13-4

Lee, Nakyeong A048,

B042, B056, B057

Lee, Nakyung D071

Lee, Nam Ki GS3-11

Lee, Nayoung A014

Lee, Pyeong-Koo B006

Lee, Rowoon S13-4

Lee, Sang Hee F032, F033

Lee, Sang hyeok B085

Lee, Sang Hyun A059, A060,

B115, B116

Lee, Sang Jun GS4-1, S4-4,

GS4-6, GS4-10, G027,

G038, G052, G053, G055

Lee, Sang Suk B103, B104

Lee, Sang Woong G002

Lee, Sang-Ah B119

Lee, Sang-Jae S6-6, GS1-3, A057,

E029, E031, E033, G056

Lee, Sang-Nam C008

Lee, Sang-Seob A034, A040,

A049, B027, B028,

B062, B063, G022, G028

Lee, Sang-Suk B097

Lee, Sang-Yeob H017

Lee, Se Hee C006

Lee, Seok Hui B054

Lee, Seong Hyuk S16-4, G005

Lee, Seong Shin H035

Lee, Seong-Chan H019

Lee, Seong-Hwan G043

Lee, Seongkyeong F038

Lee, Seonju D043

Lee, Seonki S20-1

Lee, Seonmin D001

Lee, Seung Hak B066

Lee, Seung Hee C066

Lee, Seung Hui D026, D031

Lee, Seung Soon C010

Lee, Seung Yeup D018

Lee, Seungchul D012

Lee, Seunghee C054

Lee, Si Hyeon B059

Lee, Si Un F015

Lee, So Jin F015, H033

Lee, So Yeon D075

Lee, Sojin H034

Lee, Somi B072

Lee, Soo-Jeong GS1-6

Lee, Soo-Yeong A023, A024

Lee, So-Yeon C031, D076

Lee, Su Ho B054

Lee, Su Yeon B110, B132

Lee, Subin B024

Lee, Su-Bin S3-3, D011

Lee, Subin GS4-9

Lee, Sujin B023, B049, B050, D027

Lee, Sun Hee D055

Lee, Sung Dae B097

Lee, Sung Ho H022

Lee, Sung Moon A048, B042,

B056, B057

Lee, Sung-Jae F014

Lee, Sung-Kyong F039

Lee, Sungkyoung H039

Lee, SungKyung F037

Lee, Sung-Mok G005

Lee, Sunjoon B055, C038

Lee, Suyeon S20-1

Lee, Tae Kwon S20-2, B009,

B071, B098, B134, B135, B138

Lee, Tae-Young D056

Lee, Ui-Ju S7-1

Lee, Wan-Kyu C017, C020

Lee, Weon Ju A020

Lee, Wonjun GS2-2, A029, B045

Lee, Wonjune GS2-6

Lee, WonJune H051

Lee, Wonsik S2-5, GS2-4,

D001, D002

Lee, Wonsuk S5-4

Lee, Woo Ghil S8-3

Lee, Ye JI B137

Lee, Yebeen D072

Lee, Ye-Eun B031

Lee, Yeji G007

Lee, Yelim B124

Lee, Yeong-jin D033

Lee, Yeseul GS2-8

Lee, Yong Jin D027

Lee, Yonghyun C063

Lee, Yongjae S6-5, F001

Lee, Yong-Jik E031

Lee, Young Seok B020, C007

Lee, Young-Ho S16-4

Lee, Younghoon G003

Lee, Youngmi A049

Lee, Yun Ha D035, D038

Lee, Yun Ju S9-1, B079

Lee, Yuna A064

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Lee, Yung Mi B011

Lee, Yunmi GS2-4

Lee, Yu-Ri D024

Li, Huiyan D032, E012

Li, JiangYu C072

Li, Lanxin S2-1

Li, Wen-Jun S7-2

Li, Xi-Hui D041

Li, Xi-hui D054

Lim, Bu-Geon D017

Lim, Chae Lim GS2-8

Lim, Da Jung S9-4

Lim, Gippeum Joy S18-1

Lim, Hyun Gyu S6-3

Lim, Hyung Woo D055

Lim, Jae Kyu S7-4, B137, F025

Lim, Jae-Hwan S8-2

Lim, Ji Hyun GS3-5, D045

Lim, Ji-Hwan B088

Lim, Jin-A H035

Lim, Jonathan GS4-7

Lim, Kyeongmo B084, B092, B143

Lim, Mi Young D029

Lim, Myeongwon H049

Lim, Sangyong F011

Lim, Seeun B010

Lim, So Yun D040, F012

Lim, SoJeong C072

Lim, Suk-Kyung F040

Lim, Sun Gyo S10-1

Lim, Tae-Gyu S5-2

Lim, Yeonjung B036

Lim, Young Woon GS2-2,

A029, B045

Lim, Younghyun S3-6

Lin, Yuxi S16-4

Liu, Shuang-Jiang (SJ) S10-4

Lolita, Gracia Pradnya A055

Low, Wen Wen S16-1

Lu, Xueqin B027, B028

Lyou, Eun Sun B009, B135

Ma, Jihyeon G037

Ma, Seong Hyeok GS3-8

Ma, Zeshi F041

Maharshak, Nitsan D063

Mahra, Kanika GS1-1, C082

Maung, Chaw Ei Htwe C068

Md, Shamsuzzamn D050

Megonigal, Patrick B082

Mehrshad, Maliheh S10-3

Miguel, Michelle A. B103

Miguel, Michelle B104

Min, Jihong F005

Min, Jihyeon S15-4, GS2-7, B142

Min, Jin Woo C015

Min, Jisu H047

Min, Kyunghun S13-4

Mitchell, Robert J. A031, G011

Mitchell, Robert James C022,

C024, E022

Miyanaga, Kazuhiko G021

Moon, Bo-Youn F040

Moon, Dong-Chan F040

Moon, Ha Jung A049

Moon, Heeji S13-4

Moon, Hye Yun C027, C049

Moon, HyeKyeong D010

Moon, Jeongsup B067

Moon, Jin-San D003, D022, D081

Moon, Jong Kook F015, H033

Moon, Jongkook H034

Moon, Jun-Ok C039, C050,

C052, D030

Moon, Kira A048, B042,

B056, B057

Moon, Seongjoon E016, E019

Moon, Taeung D065

Moon, Yea Lin A051, B139

Mota, Catia S3-3, D011, H043

Mulholland, Adrian S11-1

Mun, Chae Won H029

Mun, Seung hyun B037

Mun, Seunghyeon B012

Mun, Wonsik G011

Myeong, Nu Ri B013

Myoung, Jinjong S12-2, H037,

H042, H046, H047

Myung, Heejoon D047

Na, Go Eun D055

Na, Hae Young D055

Na, Jung-Hyun E034

Na, Sang Hoon B103

Na, Soo-Chang C040

Naing, Ye Pyae B104

Nam, Seohyun C067

Nam, Gaewon B021

Nam, GaeWon E029, E033

Nam, Gi Heum C028

Nam, Gijeong C049

Nam, Jae Yun F037, F039

Nam, Jaeyun F038

Nam, Seo-Hyeon G026

Nam, Sungjin B141

Nam, Young-Do S1-2

Naskar, Manisha D009

Ngo, Hoan D060

Nguyen, Bao Ngoc C002

Nguyen, Loan Thi Thanh A011

Nguyen, Thi Mai Tho B113

Niu, Wenxin D032

Noh, Choong Hwan B076, B086

Noh, Donghyun C061

Noh, Eui-Jeong F037, F039

Noh, Eui-jeong F038

Noh, Hyun-Ju A039

Noh, Jihye B123

Noh, Jun Su C068

Noh, Yoonjoo GS1-10, B114, B117

Noyce, Genevieve B082

Oh, Byeong Seob C037

Oh, Chang Wan F015

Oh, Eun Ryul B055

Oh, EunRyul C038

Oh, Eunseon C052, D030

Oh, Han Byeol GS2-8

Oh, Han Na S10-1

Oh, Hyo Bin G020, G023

Oh, Hyun Cheol B125, B126

Oh, Hyun-Myung B090

Oh, Hyun-Seok B067

Oh, Jae Young B081, B087, B091

Oh, Jaejun B043

Oh, Jeong-Il E027, E028, E030

Oh, Ji-Yeon G012

Oh, JongSeok D010

Oh, Ju Kyoung C039

Oh, Juyoung F022

Oh, Mihee B010

Author Index

www.msk.or.kr | 303

Oh, Mi-Hwa C076

Oh, Minhee E010

Oh, Miyoung G013

Oh, Nam Su S14-3

Oh, Namsu C071, C073, G042

Oh, NamSu C072

Oh, Se Jong D027

Oh, Seung-Yoon GS1-10,

B114, B117

Oh, So-yeon C019, H016

Oh, Su Min G029, G030

Oh, Young Taek H011

Oh, Yuna E027

Ohkuma, Moriya S7-3

Oyuntsetseg, Bilguun A063

Palsson, Bernhard O. F001

Parekh, Viraj D009

Park, Ah Jeong D042

Park, Anyeseu F005

Park, Anyeseu GS3-6

Park, Bongju S8-5

Park, Chan-Hyeok GS3-12,

A013, D024

Park, Chan-hyeok D014

Park, Chanwoo D027

Park, Cheolju B012, B037

Park, Daool H036

Park, Doo-Sang GS3-12, A013,

D014, D024

Park, Eun-Jin B032, D006

Park, Gwang-Hyun S11-2

Park, Gyulim B020, C007

Park, Hee-Soo A054, F024, G046

Park, Hee-Yeon B062, B063

Park, Heung Sik B137

Park, Hi-Joon B050

Park, Hye Yoon F007

Park, Hyein B049

Park, Hyejin G005

Park, Hyemin S8-5

Park, Hyerin H008

Park, Hyun Bong F015

Park, Hyun-Jun G043

Park, Jae Young A039

Park, Jaehyo B049

Park, Jam-Eon GS2-9, C079

Park, Jang Woo D027

Park, JeeYoon B010

Park, Jeong-Hwa D017

Park, Jeong-Hyun A066

Park, Jeongseok GS3-7

Park, Ji gwon C019

Park, Ji Heon GS3-5, D045

Park, Ji Hye S16-4

Park, Ji Yeong GS1-3, A057, E029,

E031, E033, G056

Park, Ji-Gweon B066, H016

Park, Jihye GS2-1, D083

Park, Jin D066

Park, Jin-Sook A050, A051,

A053, B070, B139

Park, Jisoo B110

Park, Jiwon C017, C020

Park, Ji-Woo B031, B032

Park, JiYeon GS2-5, C084

Park, Jiyeun S13-4

Park, Jiyoung H006

Park, Jong Seong G029

Park, Jong-Eun D056, G007

Park, Jong-Heum H014

Park, Joong Kook B012

Park, Joon-Young B029, B047,

C009

Park, Ju Yeon S18-1

Park, June-Kyu D033

Park, Jung Eun H003

Park, Junhyuk H032

Park, Jun-Sun D040

Park, Jun-sun F012

Park, Jun-Young D046

Park, Keun Ha F028

Park, Ki Hyeong A036, B052, C053

Park, Ki-Tae GS4-3

Park, Kwang Hyun G003

Park, KyeongSim D010

Park, Mi Jung D021

Park, Mi-Hwa A057, E029,

E031, E033

Park, Mi-Jeong S7-4, A015,

B076, B086, E014, F006, F016

Park, Mijung D020

Park, Min Ah C039

Park, Min-Kyu C048

Park, Mirieom S9-4

Park, Miyeon C020

Park, Rye Gyeong B125, B126,

H048

Park, Sang Geun E033

Park, Seo Hyun E017

Park, Seol Hwa B097

Park, Seon Young D026, D031

Park, Seon-Ju F018

Park, Seowoo S8-5

Park, Seung-Hwan S9-3, GS2-9,

C079

Park, Seung-Moon G054

Park, Sihyun C058

Park, Sinseong C022, C024, G011

Park, So Hee B138, D058

Park, So Hyeon B127

Park, So Young C025, F004

Park, Sohee GS2-8

park, So-Hyeon D046

Park, Sohyun D021, D084

Park, Somi C015

Park, Sook D048, D055

Park, Soyeon S9-4, B046, C035

Park, Subin D046

Park, Sung-Gyu H029

Park, SungHyun D061

Park, Sung-won G037

Park, Sunho A001, A002, A003,

A004, A005, A006, A007, A008

Park, Taeyoon B013

Park, Tan Sol G015

Park, Uni S12-5, D072

Park, Woncheoul H035

Park, Woojun S15-4, GS2-3, GS2-7,

B025, B142, D082

Park, Yeong Dae B054

Park, Yeong-Jun A014, C048, A015

Park, Yeon-Joon F035

Park, Yeonng-Jun S7-4

Park, Yerim S15-4, GS2-3,

GS2-7, D082

Park, Yon Koung D021

Park, Yongsoo D029

Park, YonKoung D020

Park, Yoonkyung D025

Park, Young Bin H022

Park, Yu Lim A042

Park, Yujin F014

Park, Yulim A041

Park, Yumi D033

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Park, Yun-Ji B130

Peng, Yuxin A041, A042

Pham, Ha E037

Pham, Thi Thu Ha C012, C026

Pradeep, Roshan F037, F038, F039

Priscu, John B019, B022

Qiao, Yuan S2-1

Ra, Ye-Eun E007

Raman, Jegadeesh C029, C030

Ramanayake, Ashansa F037,

F038, F039

Rhee, Sung-Keun S7-1

Rho, Ami B110, B132

Ro, Hyo-Jin D072

Ro, Jaeyoung B023

Roe, Jung-Hye S16-3

Roh, Eunjung D018

Roh, Taehun C059

Ryou, Jungsang H018

Ryu, Bumhan GS2-8

Ryu, Byung Hee H002

Ryu, Chaehwa B097

Ryu, Heeju S12-4

Ryu, Jeoungjin G031

Ryu, Ji-Hwan S1-3

Ryu, Sangdon A048, B056, B057

Ryu, Sang-don B042

Ryu, Sangryeol S4-1

Ryu, Seoung Woo C037

Ryu, Sumin G008

Sa, Hyunwook B015

Sadowsky, Michael J. PL1

Sahastrabuddhe, Sushant S18-1

Sang, Hyunkyu G037

Sapkota, Asmita D006

Seddon, Chloe S16-1

Sekendar, Ali Md F040

Semrau, Jeremy D. S15-1

Seo, Chaeryeon B078

Seo, Chang Wan A029

Seo, Do Won F039

Seo, DoWon F037

Seo, Dowon F038

Seo, Eun-Su B101

Seo, Hyomin A026, A027,

A028, A062, B044

Seo, Jae-Gu C008

Seo, Jeong Mi D033

Seo, Jinbeom D001

Seo, Jin-Hee D027

Seo, Jiwon A056

Seo, Ji-Won A061

Seo, Jung Ah C043

Seo, Jun-Hyeong B055,

C038, H022

Seo, Kangmin H017

Seo, Kwangwon C017, C020

Seo, Kyoung Sun H028,

H030, H031

Seo, Mi-Ran F021, F029, F036

Seo, Myung-Ji B096

Seo, Sang-Tae A036, C053, B052

Seo, Seongwon D026

Seo, Sun Il B088

Seo, Taegun A001, A002, A003,

A004, A005, A006, A007, A008

Seo, Yerin B050

Seo, Young-Jin S3-6

Seo, Young-Su D012

Seok, Yeong Jae E008

Seok, Yeong-Jae GS2-12, A016,

C011, E003, E015

Seong, Hyeri C055

Seung, Geum Ji D052

Seung, Youjin E028

Shamsuzzaman, Md A010, C003

Shao, Hongfei H007, H010

Shim, Sang Min G047

Shim, Young Kun G037

Shim, Youngkun B043

Shin, Cheol Min C061

Shin, Eun Kyoung S17-4

Shin, Eunkyoung S3-1, F003

Shin, Eunkyung C012, D058

Shin, Hae Soo F007

Shin, Hakdong S5-2, S14-4,

GS2-6, GS2-10, C057,

C059, C061, C062, H051

Shin, Ho-Young A064, B128

Shin, Hoyoung B131

Shin, Hyunki E007

Shin, Jae-Ho GS1-1, GS1-4,

GS2-5, GS2-11, GS3-3, GS3-9,

B084, B092, B143, C034, C041,

C045, C048, C051, C058, C081,

C082, C083, C084, F010

Shin, Jae-Hyeong A066

Shin, Jeong-Ih F021, F035, F036

Shin, Jieun S8-5

Shin, Ji-Hee S1-2

Shin, JiHye D010

Shin, Ji-Yoon A051

Shin, Jiyoung S13-4

Shin, Joo Hun A020

Shin, Kihyuk D078

Shin, Kwang-Soo S8-5

Shin, Kyuwon D077

Shin, Mi Yeong D048

Shin, Minsang D050

Shin, Myeonghee H044, B002

Shin, Raan B023

Shin, Seo Hyeon C027

Shin, Seung Yeol A026, A027,

A028, A062, B044

Shin, Seyeon B005

Shin, Young Joo B029

Shin, Youngsun D054

Shin, Yuji C052

Shin, YuJin D002

Shin, Yuna A011

Shinohara, Akira S3-4

Shohel, Md Rana D050

Shohel, Rana Md A020, F002

Si, Jiyeon C002

Sim, Chaemin C018

Sim, Sanghyeob C040

Singh, Vineet GS1-1, GS2-11,

C082, C083

Sliti, Amani GS3-3, C041

So, Kum-Kang GS3-4, G041, G054

So, Kyoung Min H017

So, Soon-Yong D066

So, YoHan B013

So, Yoonseop A008

Sohn, Jae Hak B125, B126, H048

Son, A Rang B103, B104

Son, Dahyeon E008

Son, Hokyoung S13-4

Author Index

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Son, Hong-Joo B020, C007

Son, HyunWoo C051

Son, Jaeyoung C055

Son, Ji eun E006

Son, Jino A045, A061

Son, Ki Hyun B125, B126

Son, Min A S9-1, B079

Son, Mi-Young GS3-12, D014

Son, Moonil H040, H041,

GS4-2, GS4-8

Son, Naeun GS3-12, D014

Son, Sang-Yong S10-1

Son, Seoyun C035

Son, Se-Young S16-4, GS3-11

Son, Ye Ji S3-3, D011

Son, Ye Seul GS3-12, D014

Son, Yongjun S15-4, GS2-3,

GS2-7, D082

Son, Young-Hee B130

Son, Yujin GS1-3, A057, G056

Song, Beom-Seok H014

Song, Boyeong S8-5

Song, Da-Young B130

Song, Eun-Ji S1-2

Song, Gyu Yong D006

Song, Ha-Yeon C074, C075

Song, Hokyung S20-4, B008

Song, Hwayoung H024

Song, Hye Seon A048, B056, B057

Song, Hyemin D047

Song, Hyesun B042

Song, Jaeho A026, A027,

A028, A062, B044

Song, Jaekyeong H020

Song, Jaeyong B012

Song, Ji Ho S9-1, B079

Song, Ju Yeon B049

Song, Ju-Hyun A066

Song, JuHyun F034

Song, Ju-Yeon B101

Song, Lee D015

Song, Manki S18-1

Song, Min Joon B140

Song, MinjiS H035

Song, Saemee E010

Song, Seonwoo D064

Song, Seung Hui B042

Song, Seung-Hui A048

Song, Seunghui B056, B057

Song, Yo Han C025

Song, Young-Jin E035

Srinivasan, Sathiyaraj A034,

A040, A049

Strynadka, Natalie C.J. PL4

Sugano, Takashi G021

Suh, Min Kuk A069

Sul, Woo Jun S10-1, B061, B068,

B069, B078, B080, B081,

B083, B102, B108, B129

Sun, Jisu D027

Sung, Dayoung D007

Sung, Hojun D019, H050

Sung, Hyuna GS3-2

Sung, Jae-Yoon S6-6, A057, G050

Sung, Minje F014

Sung, Min-Kwan G040

Taj, Mehwish B119

Tak, Euon Jung H050

Tan, XinEe G021

Tanudjaja, Ellen E001

Thak, Eun Jung S3-3, D011, D064

The, Hung Trinh A066

Thi, Trinh Nguyen G001

Thitiananpakorn, Kanate G021

Tissera, Kavinda D005

To, Uyen Tran Thi H009

Toyofuku, Masanori S15-3, S15-4

Trinh, Nguyen Thi E007

Tsujii, Masaru E001

Um, Jihye D040, F012

Um, Jiyeon D051, D059

Unno, Tatsuya S20-4, B008, B072

Uozumi, Nobuyuki E001

Veeranarayanan, Srivani G021

Wang, Kun F041, G043

Watanabe, Shinya G021

Weon, Hang Yeon B109, B111

Weon, Hang-Yeon B075,

B077, H020

Whang, Jake D033

Whon, Tae Woong S1-4, C031

winkler, Alexandra E012

Wong, Joshua S16-1

Woo, Eu Jeon G003

Woo, Eui-Jeon S11-2

Woo, Haejin A001, A002, A004,

A005, A006, A007, A008

Woo, Hyunjae G034

Woo, Jung-Jae GS1-10

Woo, Sungho B011

Wook, Hong Gi B053

Yakubu, Ismaila S9-1, B079

Yamamoto, Kaneyoshi E001

Yang, Baul C016

Yang, Dong-Hoon S13-3

Yang, Gwon Mo C025, D028

Yang, Gyeongjun C017

Yang, Haneol GS3-12, A013,

D014, D024

Yang, Hui-wan S3-2

Yang, Hye Lin D048

Yang, Hyun Sung B137

Yang, Jae Seung S18-1

Yang, Jeong Seong D033

Yang, Jhung-Ahn S7-4

Yang, Jiho B006, B007

Yang, JiHye H014

Yang, Jihye S15-4, GS1-8, GS2-3,

C021, D082

Yang, Ji-in E011

Yang, Joo Young GS1-3, A057,

E029, E031, E033, G056

Yang, Joo-Sung S8-4

Yang, Jun Hyeok F015, H033

Yang, Jungeun G032, G033

Yang, Jung-Wook S13-4

Yang, Junhyeok H034

Yang, Seongeun S9-2

Yang, Soo-Jin GS3-5, D045

Yang, So-Yeon H045

Yang, Su-Been S6-1

Yang, Sung Hyun S7-4

Yang, Sung-Hyun A014, A015

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Yang, Yerang B082

Yang, Yoon Mo E017

Yang, Yoon-Mo S16-2

Yang, Yoonyong B099, F007

Yang, Youri C018, C033

Yang, Yubin S3-1, F003

Yeo, Jinah D056, G007

Yeom, Jinho G029

Yeom, Jinki D053, D060, D080

Yeom, Seo-Joon H014

Yeom, Soo-Jin S11-4

Yeon, Jehyeong B075, B077, B109

Yeon, Jehyung B111

Yi, Soojung GS3-11

Yim, Hong soon D040

Yim, Hongsoon F012

Yim, Jae-Joon D033

Yim, Sung Sun S6-2

Yoo, Ho-Seok G048

Yoo, In Young F035

Yoo, Ji Ho D073

Yoo, Ji-Seung S12-3

Yoo, Jong Hee G020, G023

Yoo, Keunje B098

Yoo, Kirim D039, G016

Yoo, Seung Min F028, G029,

G030, H027, H029

Yoo, Siyoung D077

Yoo, Su Jin C043, C056, H043

Yoo, Su-Kyoung G049

Yoo, Suyeon C077, C078

Yoo, Woongjae S17-1

Yoo, Yeonjae A059, A060,

B115, B116

Yoo, Yesol C013

Yoo, Yongjae H001

Yook, Subin A001, A002, A003,

A004, A005, A006, A007

Yoon, Chang Ki B029

Yoon, Ga-Yeon S12-5

Yoon, Hae-Eun G012

Yoon, Hongseop C042

Yoon, Hongsuop C046

Yoon, Hongsup C044, D063

Yoon, Ho-yeong H037

Yoon, Hyunjin GS3-1, GS3-7,

D047, D077

Yoon, Jang Won B113, D067

Yoon, Jeong-Ah G036

Yoon, Jeong-Kee S3-3

Yoon, Jiyoung GS4-3

Yoon, Jonghwan G039

Yoon, Jung Ah G045, G047

Yoon, Jung Hoon E005, E023

Yoon, Jung-Ah G026

Yoon, Sang Eun D019

Yoon, Sang Sun S19-1,

GS3-2, GS3-8, D074

Yoon, Seobin S3-4

Yoon, Sojung B035

Yoon, Su-In B032

Yoon, Sukhwan B035, B051,

B074, B133, B140

Yoon, Sung Ho G017, G034, G035

Yoon, Sung Won H023

Yoon, Sung-Ho F034

Yoon, Sung-il GS2-8

Yoon, Sunkyung D039, G016

Yoon, Sunmi B010

Yoon, Taeho B051

Yoon, Weon-Jong B066, C019,

H015, H016

Yoon, Yeo Joon GS3-11

Yoon, Youngyoon G019

Yoon, Yurim E010

You, Jin Sun D074

You, Su-Hyun D017

Youn, Jae Uk B134

Youn, So-Yeon GS3-11

Yu, Hyo Jin D048

Yu, Jae-yon A040, G028

Yu, Jeonghwa E021

Yu, Jihyun B076, B086, B106

Yu, Jin-Gyeong D068

Yu, Junsun C002

Yu, Seung Yeob C037

Yuk, Seongje H040

Yum, Seungshic A014

Yun, Chan-Seok D024

Yun, Chi Sun D022

Yun, Hocheol H006

Yun, Jin-seung D058

Yun, Ki Na F015, H033

Yun, Kina H034

Yun, Kyeng-Won H031

Yun, Kyeong-Won H028, H030

Yun, Mi-ran D058

Yun, Misun C005, C006

Yun, Seok-Min H018

Yun, Taegwan D002

Yun, Taeho B140

Yun, Yeo-Eun B022, B032

Zhao, Mengchen B028

Zhen, Guangyun B027

Zhuo, Ye A067, A068, B136

2024

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